Methylcellulose Stock Solution from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMethylcellulose Stock Solution
CompanyR&D Systems, a Bio-Techne Brand
Price $183.00Supplier Page View Company Product Page
Catalog NumberHSC001
Quantity100 mL
StorageStore the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Promotion of breast cancer by the DNPH1 enzymeiScienceApril 17, 2026Sangphil Oh, Yuan Sui, Sook Shin, Ralf Janknechtfactor,Peprotech,Cat# 100-18B-100 Heparin,Sigma-Aldrich,Cat# H3149 Methylcellulose,R&D Systems,Cat# HSC001 DMEM/F-12 media,ThermoFisher-Gibco,Cat# 11320033 "Media supplement B-27 (50x), serum free",ThermoFisher-Gibco,Cat#

(2) LIN28A-Dependent Kinome and Phosphoproteome Reprogramming Promotes Imatinib ResistanceMolecular & Cellular ProteomicsJanuary 19, 2026Owen F.J. Hovey, Mallory I. Frederick, Quan M. Quach, Jenica H. Kakadia, Alyssa Wu, Kyle Yang, Tingting Wu, Xiang Ruan, Tomonori Kaneko, Courtney Voss, et al.1000 cells were suspended in IMDM medium containing 10% FBS and 0.9% methylcellulose (R&D Systems, HSC001), with or without 0.5 μM imatinib. The cell suspension (1 ml/well) was dispensed into 24-well plates

(3) Optimization of velocity receptor transduction in CAR T cellsbioRxivOctober 8, 2025Xinyun Jiang, Vasco Queiroga, Eban A. Hanna, Denis WirtzT cells were added per well in 100 μL viscous X-VIVO 15 containing 10% methylcellulose (R&D Systems, HSC001), 5% human serum, 1% penicillin– streptomycin, and 1% L-glutamine with IL-2 at 100 IU/mL and TransPlus

(4) Aquaporin 5 expression regulates MDA-MB-231 spheroid multicellular invasionAmerican Journal of Physiology-Cell PhysiologyAugust 20, 2025Ian M. Smith, Autumn C. Hengen, Ariel W Abraham, Sai Pranav Majeti Majeti Venkata, Shohini Banerjee, Nikka Givpoor, Allison K. Moses, Kimberly M. Strokasplitting, cells were suspended in a more viscous DMEM media, supplemented with 0.25% methylcellulose (HSC001, R&D Systems) to encourage clustering, and were seeded in a 96-well round-bottom ultra-low adhesion

(5) Gut dysbiosis conveys psychological stress to activate LRP5/β-catenin pathway promoting cancer stemnessSignal transduction and targeted therapyMarch 5, 2025Cui B, Luo H, He B, Liu X, Lv D, Zhang X, Su K, Zheng S, Lu J, Wang C, et al.C11330500BT) with 20 ng/ml epidermal growth factor (Sigma, E9644), 1% methylcellulose (R&D Systems, HSC001), 20 μg/ml B27 (Gibco, 17504044) and 20 ng/ml basic fibroblast growth factor (Peprotech, 100-18B). Every

(6) Viscosity regulates cell spreading and cell‐extracellular matrix interactionsThe FEBS JournalFebruary 1, 2025Hugh Xiao, Xiangyu Gong, Seyma Nayir Jordan, Zixie Liang, Michael Maksequential addition The 3% methylcellulose (MC) stock solution in IMDM was bought from R&D Systems (cat. #HSC001, Minneapolis, MN, USA). To achieve 1% MC, the stock solution was diluted with IMDM (Life Technologies/Gibco,

(7) Extracellular fluid viscosity regulates human mesenchymal stem cell lineage and functionScience advancesJanuary 3, 2025Amitrano A, Yuan Q, Agarwal B, Sen A, Dance YW, Zuo Y, Phillip JM, Gu L, Konstantopoulos K.media with viscosities of 3, 4, 6, and 8 cP, 20 ml of 0.76, 0.90, 1.10, and 1.24% methylcellulose (HSC001, R&D Systems), respectively, in IMDM was mixed with an equal volume of adipogenic and osteogenic inductive

(8) Lung-resident alveolar macrophages regulate the timing of breast cancer metastasisCellNovember 14, 2024Erica Dalla, Michael Papanicolaou, Matthew D. Park, Nicole Barth, Rui Hou, Deisy Segura-Villalobos, Luis Valencia Salazar, Dan Sun, Alistair R. R. Forrest, Maria Casanova-Acebes, et al.penicillin and 100ug/ml streptomycin (Corning)) supplemented with 0.25% methylcellulose (R&D systems HSC001). Sphere-forming capacity was measured by quantification of number of spheres per well after 7 days

(9) Optogenetically engineered Septin-7 enhances immune cell infiltration of tumor spheroidsProceedings of the National Academy of Sciences of the United States of AmericaOctober 29, 2024Chen J, Hnath B, Sha CM, Beidler L, Schell TD, Dokholyan NV.On day 0, we seeded 5,000 cells in 20 μL RPMI media containing 0.25% methylcellulose (R&D Systems, HSC001) on the lid of a 100 mm petri dish. We added 10 mL sterile water to the petri dish turned over the lid

(10) Combined targeting of GPX4 and BCR-ABL tyrosine kinase selectively compromises BCR-ABL+ leukemia stem cellsMolecular cancerOctober 28, 2024Zeng C, Nie D, Wang X, Zhong S, Zeng X, Liu X, Qiu K, Peng X, Zhang W, Chen S, et al.Subsequently, the cells were cultured in IMDM supplemented with 2% FBS and Methylcellulose Stock Solution (HSC001, Bio-Techne). Cultures were incubated at 37 °C in a humidified atmosphere of 5% CO2 for 10 to 14 days

(11) Redundant but essential functions of PARP1 and PARP2 in DNA ligase I-independent DNA replicationNucleic acids researchSeptember 23, 2024Bhandari SK, Wiest N, Sallmyr A, Du R, Tomkinson AE.

(12) Integrin αvβ3 Upregulation in Response to Nutrient Stress Promotes Lung Cancer Cell Metabolic PlasticityCancer researchMay 15, 2024Nam A, Jain S, Wu C, Campos A, Shepard RM, Yu Z, Reddy JP, Von Schalscha T, Weis SM, Onaitis M, et al.create a single-cell suspension. Then, 1 mL of methylcellulose stock media was added (R&D Systems, HSC001) to a 24-well non-treated plate. Then, 1 mL of the indicated media containing 4000 cells were added.

(13) Newly identified roles for PIEZO1 mechanosensor in controlling normal megakaryocyte development and in primary myelofibrosisAmerican journal of hematologyMarch 1, 2024Abbonante V, Karkempetzaki AI, Leon C, Krishnan A, Huang N, Di Buduo CA, Cattaneo D, Ward CM, Matsuura S, Guinard I, et al.enrichment kit (Stem Cell Technologies, cat# 19856) were encapsulated in 2% MC gel (R&D systems, cat# HSC001) at room temperature at a concentration of 1x106 cells/mL in the presence of TPO (50 ng/mL (Stem Cell Technologies

(14) METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translationJournal of hematology & oncologyFebruary 1, 2024Xue M, Dong L, Zhang H, Li Y, Qiu K, Zhao Z, Gao M, Han L, Chan AKN, Li W, et al.

(15) Technical report: liquid overlay technique allows the generation of homogeneous osteosarcoma, glioblastoma, lung and prostate adenocarcinoma spheroids that can be used for drug cytotoxicity measurementsFrontiers in Bioengineering and BiotechnologyOctober 6, 2023Camille Jubelin, Javier Muñoz-Garcia, Denis Cochonneau, Emilie Ollivier, François Vallette, Marie-Françoise Heymann, Lisa Oliver, Dominique Heymanncultures using HD, cells were suspended at a concentration of 1 × 106 cells/mL. A methylcellulose (HSC001, R&D System, Abington, UK) solution diluted extemporaneously (1/2) with complete culture medium was

(16) Pancreatic cancer cells upregulate LPAR4 in response to isolation stress to promote an ECM-enriched niche and support tumour initiationNature cell biologyFebruary 1, 2023Wu C, Rakhshandehroo T, Wettersten HI, Campos A, von Schalscha T, Jain S, Yu Z, Tan J, Mose E, Childers BG, et al.the Trypan blue exclusion assay. Briefly, 4000 cells were mixed in 1mL methylcellulose stock media (HSC001, R&D systems, Minneapolis, MN) in 24-well non-treated plate (Corning), topped by 1 mL 10% regular FBS-

(17) Targeting PEA3 transcription factors to mitigate small cell lung cancer progressionOncogeneFebruary 1, 2023Shia DW, Choi W, Vijayaraj P, Vuong V, Sandlin JM, Lu MM, Aziz A, Marin C, Aros CJ, Sen C, et al.assay Cells were single cell dissociated and resuspended in a suspension of 1% methylcellulose (R&D Systems, HSC001) in standard RPMI to a final density of 5 × 104 cells/mL. 1 × 105 cells were seeded into 12-well plates

(18) Metabolomic and Mitochondrial Fingerprinting of the Epithelial-to-Mesenchymal Transition (EMT) in Non-Tumorigenic and Tumorigenic Human Breast CellsCancersDecember 16, 2022Cuyàs E, Fernández-Arroyo S, Verdura S, Lupu R, Joven J, Menendez JA.

(19) Extracellular fluid viscosity enhances cell migration and cancer disseminationNatureNovember 1, 2022Bera K, Kiepas A, Godet I, Li Y, Mehta P, Ifemembi B, Paul CD, Sen A, Serra SA, Stoletov K, et al.

(20) Theoretical and Experimental Investigation of Alginate Microtube Extrusion for Cell Culture ApplicationsBiochemical engineering journalJanuary 1, 2022Nusterer M, Rauch J, Viljoen H.in Figure 2(c). 2.2 Materials Reagents and their suppliers: Methylcellulose stock solution (cat # HSC001, R&D system), Sodium Alginate (cat # 194-13321, 80~120cp, Wako Chemicals), Calcium Chloride Dihydrate

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