pCI-neo Mammalian Expression Vector from Promega

Product Specs
ItempCI-neo Mammalian Expression Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1841
Selectable MarkerAmp
Quantity20microg
Molecular Weight~88kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepCI-neo

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Characterization of cardiac disease-associated mutations in RyR2 Ca 2+ - and caffeine-binding sitesAmerican Journal of Physiology-Cell PhysiologyApril 1, 2026Venkat R. Chirasani, Akanksha Patwardhan, Naohiro YamaguchicDNAs Full-length rabbit RyR2 (9) were cloned into the mammalian expression vector pCIneo (Cat. No. E1841, Promega). Single and multiple base changes were introduced by Pfu-turbo polymerase-based chain reaction,

(2) Overexpression of JMJD6 drives immune evasion via the BRD4–IRF1–PD-L1 axis and promotes malignancy in gastric cancerScientific ReportsDecember 8, 2025Ryo Ishida, Shuhei Komatsu, Hajime Kamiya, Takuma Ohashi, Taisuke Imamura, Jun Kiuchi, Keiji Nishibeppu, Yusuke Takashima, Hiroshi Arakawa, Masateru Yamauchi, et al.MKN45 cells, which were transfected with either an empty vector (pCI-neo Mammalian Expression Vector; E1841; PROMEGA KK) or the HaloTag-JMJD6 plasmid via Lipofectamine 3000 (Invitrogen). JMJD6 protein expression

(3) ENT3: A lysosomal urate transporter regulating urate disposition and macrophage inflammationiScienceNovember 27, 2025Isamu Matake, Tomoya Yasujima, Hirotaka Matsuo, Akiyoshi Nakayama, Yu Toyoda, Tappei Takada, Katsuhisa Inoue, Takahiro Yamashiro, Hiroaki YuasaTable S6,This paper,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pCI-neo,Promega,Cat#E1841 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, WinNonlin,Certara,https://www.certara.com/software/phoenix-winnonlin/ SigmaStat,Grafiti,https://grafiti.com/sigmastat/

(4) The harmonized activities of HER2–HER3 heterodimer and deacetylated FOXA1 evade hormone response by regulating FOXA1 chromatin bindingNucleic Acids ResearchNovember 11, 2025Shixiong Wang, Gemma Santacana-Font, Darek Kedra, Siv Gilfillan, David Tena-Chaves, Helga Bergholtz, Olav Engebraaten, Ole Christian Lingjaerde, Javier Gutiérrez-Fernández, Jens Henrik Norum, et al.anti-HER2 drugs (trastuzumab, 20 ng/ml) for 60 min. Plasmids HA-tagged FOXA1 was subcloned into pCI-neo (E1841, Promega, Madison, WI, USA) expression vector for transient transfection. Two lysines in Wing 1 (K237

(5) Discovery of ETS1 as a New Gene Predisposing to Dilated CardiomyopathyDiagnostics (Basel)August 13, 2025Zun-Ping Ke, Jia-Ning Gu, Chen-Xi Yang, Xue-Lin Li, Su Zou, Yi-Zhe Bian, Ying-Jia Xu, Yi-Qing YangQIAGEN GmbH, Hilden, Germany). The purified ETS1 cDNA and eukaryotic expression plasmid pCI-neo (Cat. No. E1841; Promega, Madison, WI, USA) were doubly cut with restriction endonucleases NheI (Cat. No. ER0971; Thermo Scientific

(6) Integrating Biochemical and Computational Approaches Reveal Structural Insights in Trastuzumab scFv-Fc Antibody EngineeringBiomoleculesApril 22, 2025Olga Bednova, Jessica Pougoue Ketchemen, Hazem Mslati, Mark Barok, Heikki Joensuu, Natalie Zeytuni, Francesco Gentile, Leon Sanche, Humphrey Fonge, Jeffrey Victor LeytonNEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Ipswich, MA, USA) and cloned into pCIneo plasmid backbone (Catalog # E1841, Promega, Madison, WI, USA) for further expression in mammalian cell lines. Escherichia coli DH5α cells

(7) A Strategy for Simultaneous Engineering of Interspecies Cross-Reactivity, Thermostability, and Expression of a Bispecific 5T4 x CD3 DART® Molecule for Treatment of Solid TumorsAntibodies (Basel)January 17, 2025Renhua R. Huang, Michael Spliedt, Tom Kaufman, Sergey Gorlatov, Bhaswati Barat, Kalpana Shah, Jeffrey Gill, Kurt Stahl, Jennifer DiChiara, Qian Wang, et al.trypsin cleavage site in the g3 protein [26] was used. Plasmid pCIneo (Promega, Madison, WI, USA, cat #: E1841) and ExpiCHO cells (Thermo Fisher, Waltham, MA, USA, cat #: A29133) were used for mammalian expression

(8) Therapy-induced senescent cancer cells contribute to cancer progression by promoting ribophorin 1-dependent PD-L1 upregulationNature communicationsJanuary 3, 2025Hwang HJ, Kang D, Shin J, Jung J, Ko S, Jung KH, Hong SS, Park JE, Oh MJ, An HJ, et al.was obtained from pGIPz-shPD-L1/Flag-PD-L1 and cloned into pCI-neo vector (Promega, Madison, WI, USA, E1841). The construct was confirmed using DNA sequencing. N- glycan release and enrichment N-glycans were

(9) De novo designed YK peptides forming reversible amyloid for synthetic protein condensates in mammalian cellsNature CommunicationsOctober 18, 2024Takayuki Miki, Masahiro Hashimoto, Hiroki Takahashi, Masatoshi Shimizu, Sae Nakayama, Tadaomi Furuta, Hisakazu Miharaexpression of YK-proteins in mammalian cells, the genes were cloned into a pCI-neo vector (Promega, E1841) by standard restriction digestion and ligation70. For the construction of NES-peptide-FP and YK-sfGFP,

(10) Expression of RNautophagy/DNautophagy-related genes is regulated under control of an innate immune receptorRNA biologyJanuary 1, 2024Fujiwara Y, Oroku K, Zhou Y, Takahashi M, Katayama T, Wada K, Tsutsumi N, Sato T, Kabuta T.products from the cDNA of the genes of interest into the pCI-neomammalian expression vector (Promega, E1841). The EGFP expression vector was prepared as previously described. For the MDA5, RIG-I, and TLR3 expression

(11) Optogenetic induction of caspase-8 mediated apoptosis by employing Arabidopsis cryptochrome 2Sci RepDecember 27, 2023Weiliang Mo, Shengzhong Su, Ruige Shang, Liang Yang, Xuelai Zhao, Chengfeng Wu, Zhenming Yang, He Zhang, Liuming Wu, Yibo Liu, et al.treatment. Materials and methods Plasmids constructs For constructing Opto-Caspase8 plasmids, a pCI (neo) (Promega, E1841) was used. Myc, Flag or GFP was inserted into pCI (neo) with EcoRI restriction site to construct pCI

(12) Translation re-initiation after uORFs does not fully protect mRNAs from nonsense-mediated decayRNAJune 1, 2023Paul J. Russell, Jacob A. Slivka, Elaina P. Boyle, Arthur H.M. Burghes, Michael G. KearsepTet-Off All-In-One backbone at SacI and XbaI sites. The 133 bp chimeric intron from pCI-neo (Promega # E1841) was inserted into the nLuc ORF by using the Q5 Site-Directed Mutagenesis Kit. All oligonucleotides

(13) Hypoxia-Induced CD36 Expression in Gastric Cancer Cells Promotes Peritoneal Metastasis via Fatty Acid UptakeAnnals of surgical oncologyMay 1, 2023Aoki T, Kinoshita J, Munesue S, Hamabe-Horiike T, Yamaguchi T, Nakamura Y, Okamoto K, Moriyama H, Nakamura K, Harada S, et al.and XbaI; Takara Bio, Kusatsu, Shiga, Japan) of cDNA and vector (pCI-neo mammalian expression vector, E1841; Promega Corporation, Madison, WI, USA), the cDNA was ligated with the vector. cDNA expression vector

(14) StARD9 is a novel lysosomal kinesin required for membrane tubulation, cholesterol transport and Purkinje cell survivalJournal of cell scienceMarch 1, 2023Sterling FR, D'Amico J, Brumfield AM, Huegel KL, Vaughan PS, Morris K, Schwarz S, Joyce MV, Boggess B, Champion MM, et al.obtained from Dr Ta Yuan Chang (Dartmouth University) and subcloned into pCI-neo (Promega, Madison, WI; E1841), placing the EGFP sequence at the C-terminus using standard methods. A similar approach was used to

(15) A conditional Smg6 mutant mouse model reveals circadian clock regulation through the nonsense-mediated mRNA decay pathwayScience AdvancesJanuary 13, 2023Georgia Katsioudi, René Dreos, Enes S. Arpa, Sevasti Gaspari, Angelica Liechti, Miho Sato, Christian H. Gabriel, Achim Kramer, Steven A. Brown, David Gatfielddownstream of the Firefly stop codon. For the latter, the chimeric intron of the pCI-neo vector (Promega, E1841) was cloned into the 3′UTR of the prLV1 vector. The following primers were used for PCR amplification:

(16) The protective effect of a combination of human intracellular and extracellular antibodies against the highly pathogenic avian influenza H5N1 virusHuman Vaccines & ImmunotherapeuticsDecember 31, 2022Qiu Jin, Zhangyu Yao, Fangzhou Liu, Yaxuan Di, Jun Gao, Xiao ZhangMA, USA).18 The pCI-neo mammalian expression vector was purchased from Promega Corporation (plasmid #E1841, Madison, USA, 2009).19 pComb3X Lambda (PubMed GenBank AF268281) and pIgG were gifts from Carlos F Barbas.20

(17) Arabidopsis cryptochrome 2 forms photobodies with TCP22 under blue light and regulates the circadian clockNature CommunicationsDecember 1, 2022Weiliang Mo et al.-293T cell protein expression system was utilized. Myc, Flag and GFP were inserted into pCI (neo) (Promega, E1841) using the EcoRI restriction site. The coding sequences for TCP22, CRY2, PPKs, and LWDs were then cloned

(18) Hesperetin promotes longevity and delays aging via activation of Cisd2 in naturally aged miceJournal of Biomedical ScienceDecember 1, 2022Chi-Hsiao Yeh, Zhao-Qing Shen, Tai-Wen Wang, Cheng-Heng Kao, Yuan-Chi Teng, Teng-Kuang Yeh, Chung-Kuang Lu, Ting-Fen Tsailine, the linearized CISD2 BAC reporter construct and pCI-neo plasmid (Promega, Mannheim, Germany, #E1841) were co-electroporated (250 V with capacitance 500 μF) into the HEK293 cells and selected with 1 μg/mL

(19) The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complexiScienceJuly 15, 2022Zou L, Moch C, Graille M, Chapat C.pET28-His6-ZZ-NSP2107−212,This paper,N/A Plasmid: pET28-His6-ZZ-NSP2204−350,This paper,N/A Plasmid: pCI-Neo,Promega,Cat# E1841 Plasmid: pCI-Neo-V5-GIGYF2,This paper,N/A Plasmid: pCI-Neo-V5-GIGYF21−267,This paper,N/A Plasmid:

(20) Y RNAs are conserved endogenous RIG-I ligands across RNA virus infection and are targeted by HIV-1iScienceJuly 15, 2022Vabret N, Najburg V, Solovyov A, Gopal R, McClain C, Šulc P, Balan S, Rahou Y, Beauclair G, Chazal M, et al.#219089 pIFNβ-Fluc (IFN-b-pGL3),Lin et al. (2000),N/A pTK-Rluc,Promega,Cat: #E2231 pCIneo,Promega,Cat: #E1841 pCMV-RNY4,This study,N/A pU6-RNY4,This study,N/A ,, Software and algorithms,Software and algorithms,Software

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