pCI Mammalian Expression Vector from Promega

Product Specs
ItempCI Mammalian Expression Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1731
Quantity20microg
Selectable MarkerAmp
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin
Vector NamepCI

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Target-stabilized base editors enable robust high-fidelity RNA editingNature CommunicationsFebruary 25, 2026Taian Liu, Yunping Lin, Qiwei Liu, Wenhui Liao, Yunyi Lin, Yujing Zhang, Jianqing Zhang, Wenhua Cao, Lixin Yang, Zexuan Hong, et al.deaminaseE1008Q gene was cloned from MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES (124626, Addgene) and inserted into pCI (E1731, Promega) for mammalian cell expression. Oligonucleotides encoding the consensus 5′ binding loop were

(2) Lipid nanoparticle-encapsulated DNA vaccine induces balanced antibody and T-cell responses in pigs with maternally derived antibodiesJournal of VirologyNovember 25, 2025Danh C. Lai, The N. Nguyen, Giao P. Trinh, David Steffen, Hiep L. X. Vua commercial DNA synthesis service (GenScript) and cloned into the pCI plasmid backbone (Promega, #E1731) between the NheI and NotI sites. The recombinant plasmid was amplified in E. coli DH5α and purified

(3) Identification and Functional Characterization of a Novel SOX4 Mutation Predisposing to Coffin-Siris Syndromic Congenital Heart DiseaseChildren (Basel)May 7, 2025Zi Yan, Bin-Bin Dong, Yan-Jie Li, Chen-Xi Yang, Ying-Jia Xu, Ri-Tai Huang, Xing-Yuan Liu, Yi-Qing YangInc.). In brief, the HeLa cells were transiently transfected with 400 ng of empty pCI vector (cat. no. E1731; Promega Corporation), 400 ng wild-type SOX4-pCI vector, 400 ng Glu111*-mutant SOX4-pCI vector, 200

(4) Human SLAM-adapted canine distemper virus can enter human peripheral blood mononuclear cells and replicate in mice expressing human SLAM and defective for STAT1 expressionVirulenceFebruary 21, 2025Boyu Zhai, Wei Ran, Yiyang Sun, Angelita Alcos, Mengjia Liu, Jie Chen, Christopher D. Richardson, Dongbo Sun, Jianjun Zhaoof the 5804PeH and 5804PeH-VhS strains was cloned and inserted into a pCI expression vector (E1721, E1731; Promega) carrying a FLAG tag (DYKDDDDK) located at the 3’ end of the gene to generate pCI-5804PeH-Hflag

(5) Selective RNA pseudouridinylation in situ by circular gRNAs in designer organellesNature CommunicationsOctober 24, 2024Lukas Schartel, Cosimo Jann, Anna Wierczeiko, Tamer Butto, Stefan Mündnich, Virginie Marchand, Yuri Motorin, Mark Helm, Susanne Gerber, Edward A. Lemkecloning, golden gate assembly, or Gibson assembly. The efficiency reporter was cloned into a pCI vector (E1731, Promega). The dual fluorescent protein reporter (selectivity reporter) was cloned in a pBI-CMV1 vector

(6) The human alpha7 nicotinic acetylcholine receptor is a host target for the rabies virus glycoproteinFrontiers in Cellular and Infection MicrobiologyMay 21, 2024Brittany C. V. O’Brien, Shelly Thao, Lahra Weber, Helen L. Danielson, Agatha D. Boldt, Karsten Hueffer, Maegan M. Weltzinβ2, β3, and β4 nAChR subunits were previously cloned into the pCI mammalian expression vector (Cat# E1731 Promega, Madison, WI) (George et al., 2012; Eaton et al., 2014; Luo et al., 2014). The α7 subunit and

(7) Characterization of two pathological gating-charge substitutions in Cav1.4 L-type calcium channelsChannels (Austin, Tex.)December 1, 2023Heigl T, Netzer MA, Zanetti L, Ganglberger M, Fernández-Quintero ML, Koschak A.p.(Arg975Gly) and p.(Arg1299Leu); UNIPROT O60840–1) were inserted into the “pCI mammalian expression vector” (E1731, Promega) by applying splicing by overlap extension PCR. In short, the human Cav1.4 coding sequence

(8) Heterologous DNA-prime/protein-boost immunization with a monomeric SARS-CoV-2 spike antigen redundantizes the trimeric receptor-binding domain structure to induce neutralizing antibodies in old miceFrontiers in ImmunologySeptember 11, 2023Dominik Pflumm, Alina Seidel, Fabrice Klein, Rüdiger Groß, Lea Krutzke, Stefan Kochanek, Joris Kroschel, Jan Münch, Katja Stifter, Reinhold Schirmbecksynthesized by GeneArt (Thermo Fisher Scientific) and cloned into the pCI expression vector (cat. no. E1731, Promega) using NheI/NotI sites. This sequence was used to generate the other S-constructs described

(9) Antagonistic interactions between odorants alter human odor perceptionCurrent BiologyJune 1, 2023Yosuke Fukutani, Masashi Abe, Haruka Saito, Ryo Eguchi, Toshiaki Tazawa, Claire A. de March, Masafumi Yohda, Hiroaki MatsunamiDNA,Recombinant DNA,Recombinant DNA Plasmid: pCI Mammalian Expression Vector,Promega Corporation,Cat# E1731 Plasmid: pGlosensor-22F,Promega Corporation,Cat# E2301 Plasmid: pCI-human RTP1S,Saito et al,N/A Software

(10) Seizures, behavioral deficits, and adverse drug responses in two new genetic mouse models of HCN1 epileptic encephalopathyeLifeAugust 16, 2022Merseburg A, Kasemir J, Buss EW, Leroy F, Bock T, Porro A, Barnett A, Tröder SE, Engeland B, Stockebrand M, et al.experiments to express human HCN1 and mouse TRIP8b Recombinant DNA reagent,pCI (plasmid),Promega,U47119;Cat# E1731,Used for HEK293T experiments to express mouse HCN2 Recombinant DNA reagent,pmaxGFP (plasmid),Amaxa

(11) A phospholipid mimetic targeting LRH-1 ameliorates colitisCell Chemical BiologyJuly 1, 2022Suzanne G. Mays, Emma H. D’Agostino, Autumn R. Flynn, Xiangsheng Huang, Guohui Wang, Xu Liu, Elizabeth J. Millings, C. Denise Okafor, Anamika Patel, Michael L. Cato, et al.2005)",N/A Plasmid: pCMV-RenillaLUC,"(Musille et al., 2012)",N/A Plasmid: pCI empty vector,Promega,Cat# E1731 Plasmid: pCI-LRH-1,"(Solomon et al., 2005)",N/A Plasmid: pCI-LRH-1Y516A,This paper,N/A Plasmid: pCI-LRH-1K520A,This

(12) Internal Introns Promote Backsplicing to Generate Circular RNAs from Spinal Muscular Atrophy GeneGenes (Basel)June 25, 2022Diou Luo, Natalia Nikolaevna Singh, Ravindra Narayan Singhwas constructed by inserting PCR amplified products in pCI vector (Promega, Madison, WI, USA, Cat No. E1731) using cDNA and/or genomic DNA as template, as described in Supplementary Figure S1. Other expression

(13) Rixosomal RNA degradation contributes to silencing of Polycomb target genesNatureApril 7, 2022Haining Zhou et al.mutated from 5′-AGACCTAAGTTCTGTCGAA-3′ to 5′-CGGCCGAAATTTTGCAGGA-3′ and integrated into the pCI (Promega, E1731) plasmid for ectopic protein expression. For bacteria protein expression, cDNA was integrated to pGEX-6P-1

(14) Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycleNature CommunicationsDecember 1, 2021Esther Griesbach, Margarita Schlackow, William F. Marzluff, Nick J. Proudfootbacteriophage SP6 promoter sequence were cloned downstream of the T7 promoter of the pCI vector (Promega, #E1731). PCR products using T7 and SP6 forward primers were used as template for in vitro transcription. The

(15) Tau phosphorylation by glycogen synthase kinase 3β modulates enzyme acetylcholinesterase expressionJournal of NeurochemistryJune 1, 2021María‐Ángeles Cortés‐Gómez, Esther Llorens‐Álvarez, Jordi Alom, Teodoro del Ser, Jesús Avila, Javier Sáez‐Valero, María‐Salud García‐Ayllón1994); the tau triple mutant tau‐VLW also inserted into the pSG vector. The “empty” pCI vector (Cat. No. E1731: Promega) served as a negative control. We also employed a GFP‐PCI vector (Promega) as a control to

(16) Semiochemical responsive olfactory sensory neurons are sexually dimorphic and plasticeLifeNovember 24, 2020Vihani A, Hu XS, Gundala S, Koyama S, Block E, Matsunami H.the manufacturer’s guidelines. Amplified fragments were cloned into pCI expression vectors (Promega E1731) containing the first 20 residues of human rhodopsin (Rho-pCI) and were verified by sequencing. Generation

(17) IFN-γ treatment protocol for MHC-I(lo)/PD-L1(+) pancreatic tumor cells selectively restores their TAP-mediated presentation competence and CD8 T-cell priming potentialJournal for immunotherapy of cancerAugust 1, 2020Stifter K, Krieger J, Ruths L, Gout J, Mulaw M, Lechel A, Kleger A, Seufferlein T, Wagner M, Schirmbeck R.synthesized (codon optimized) by GeneArt (Regensburg, Germany) and cloned into the pCI vector (cat. no. E1731, Promega, Mannheim, Germany) using the NheI and NotI restriction sites. All antigen modifications were

(18) Herpes Simplex Virus 1 ICP34.5 Alters Mitochondrial Dynamics in NeuronsJournal of virologyJuly 1, 2020Manivanh R, Mehrbach J, Charron AJ, Grassetti A, Cerón S, Taylor SA, Cabrera JR, Gerber S, Leib DA.viruses, the mCherry open reading frame was cloned into the multiple-cloning site of plasmid pCI (Promega E1731). The BglII-BamHI region of the resulting plasmid, which contains mCherry under the CMV immediate early

(19) A non-canonical role for the EDC4 decapping factor in regulating MARF1-mediated mRNA decayeLifeJune 8, 2020William R Brothers, Steven Hebert, Claudia L Kleinman, Marc R Fabianbackbone used for selecting transfected cells with puromycin Recombinant DNA reagent,pCI-neo,Promega,E1731,For all constructs labelled ‘LNHA’ and ‘RL’ Recombinant DNA reagent,pBABE-3xFLAG-MARF1Cterm,"Nishimura

(20) Probing Morbillivirus Antisera Neutralization Using Functional Chimerism between Measles Virus and Canine Distemper Virus Envelope GlycoproteinsVirusesJuly 27, 2019Muñoz-Alía MA, Russell SJ.containing the human elongation factor was followed by a chimeric intron (pCI vector; Promega Corp, Cat. #E1731), T7opt, HHRbz, and cloning sites were synthesized and ligated into the vector. A SV40 polyadenylation

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