PolyATtract mRNA Isolation System I from Promega

Product Specs
ItemPolyATtract mRNA Isolation System I
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberZ5210
Time45 minutes
Quantity3 isolations
SourceReaction
Nucleic Acid TypemRNA

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Deletion of Tfap2a in hepatocytes and macrophages promotes the progression of hepatocellular carcinoma by regulating SREBP1/FASN/ACC pathway and anti-inflammatory effect of IL10Cell Death DisApril 3, 2025Zhiwei Li, Chun Zhang, Guixiang Huang, Zixin Zhang, Qinghao Wang, Xiran Liu, Yanling Qin, Hao Zhou, Anyi Hou, Jun He, et al.blots, total RNA was isolated to purify the mRNA according to the instructions of mRNA Isolation System (Z5210, Promega). The RNA secondary structure was destroyed at 95 °C for 3 min. mRNA was dropped onto the positively

(2) The m6A demethylase fat mass and obesity-associated protein mitigates pyroptosis and inflammation in doxorubicin-induced heart failure via the toll-like receptor 4/NF-κB pathwayCardiovascular Diagnosis and TherapyFebruary 15, 2024Weiling Tu, Xiao Huang, Songtao Liu, Yuliang Zhan, Xinyong Cai, Liang Shaoprotocol (22). Briefly, mRNA was first purified from total RNA with the PolyATtract mRNA Isolation System (Z5210, Promega, Madison, WI, USA). One-tenth of the mRNA was stored as Input. Magnetic beads were incubated

(3) Mapping of m(6)A and Its Regulatory Targets in Prostate Cancer Reveals a METTL3-Low Induction of Therapy ResistanceMolecular cancer research : MCRAugust 1, 2021Cotter KA, Gallon J, Uebersax N, Rubin P, Meyer KD, Piscuoglio S, Jaffrey SR, Rubin MA.manufacturer instructions. PolyA+ mRNA was isolated using the PolyATtract mRNA Isolation System (Promega, Z5210). Twenty microgram of mRNA was used as input for miCLIP following the previously reported protocol (31)

(4) CMTR1-Catalyzed 2'-O-Ribose Methylation Controls Neuronal Development by Regulating Camk2α Expression Independent of RIG-I SignalingCell reportsOctober 20, 2020Lee YL, Kung FC, Lin CH, Huang YS.VECTASTAIN Elite ABC-Peroxidase Kit,Vector Laboratories,"Cat# PK-7100, RRID:AB_2336827" PolyATtract mRNA Isolation Systems,Promega,Z5210 ,, Deposited Data,Deposited Data,Deposited Data ,, Microarray data for transcriptomic analyses,This

(5) MicroRNAs Are Involved in Maize Immunity Against Fusarium verticillioides Ear RotGenomics, Proteomics & BioinformaticsJune 10, 2020Zijian Zhou, Yan Cao, Tao Li, Xinghao Wang, Jiafa Chen, Hang He, Wen Yao, Jianyu Wu, Huiyong Zhangwere isolated using oligo-dT bead extraction according to the manufacturer’s instructions (Catalog No. Z5210, Promega, Madison, WI). The purified mRNAs were pooled together. Degradome sequencing was performed

(6) METTL3 and ALKBH5 oppositely regulate m⁶A modification of TFEB mRNA, which dictates the fate of hypoxia/reoxygenation-treated cardiomyocytesAutophagyAugust 1, 2019Song H, Feng X, Zhang H, Luo Y, Huang J, Lin M, Jin J, Ding X, Wu S, Huang H, et al.mRNA was first purified from total RNA using PolyATtract mRNA Isolation Systems (Promega Corporation, Z5210). Then, mRNA was denatured to 70°C for 10 min, fragmented and immunoprecipitated with anti-m6A antibody

(7) Analysis of Functional Virus-generated PAMP RNAs Using IFNα/β ELISA AssayBIO-PROTOCOLJune 20, 2019Margit Mutso, Xiang Liu, Andres Merits, Suresh Mahalingam(Roche, catalog number: 04716728001) 13. PolyATtract® mRNA Isolation Systems (Promega, catalog number: Z5210) 14. LipofectamineTM 2000 reagent (Thermo Fisher Scientific, catalog number: 11668019) 15. VeriKine Mouse Interferon Beta ELISA Kit

(8) The miR9863 Family Regulates Distinct Mla Alleles in Barley to Attenuate NLR Receptor-Triggered Disease Resistance and Cell-Death SignalingPLoS GeneticsDecember 11, 2014Jie Liu, Xiliu Cheng, Da Liu, Weihui Xu, Roger Wise, Qian-Hua Shenand mRNAs were enriched from 100 µg of total RNAs using the PolyATtract mRNA isolation kit (Promega, Z5210). The RNA Oligo adaptor was ligated to mRNAs without calf intestinal phosphatase treatment. For the

(9) Iron-dependent regulation of frataxin expression: implications for treatment of Friedreich ataxiaHuman molecular geneticsAugust 1, 2008Kuanyu Li, Edward K Besse, Dung Ha, Gennadiy Kovtunovych, Tracey A RouaultTRizol (Invitrogen). polyA RNA for northern blot was isolated with PolyATract mRNA isolation kit (cat# Z5210, Promega, Madison, WI). Northern hybridization was carried out and the blot was exposed to PhosphoImage

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PolyAttract® mRNA Isolation Kits From Promega
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