EnduRen Live Cell Substrate from Promega

Product Specs
ItemEnduRen Live Cell Substrate
CompanyPromega
Price $405.00Supplier Page View Company Product Page
Catalog NumberE6482
Quantity3.4mg
Molecular Weight~81kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Human antibodies against West Nile and related orthoflavivirusesbioRxivApril 6, 2026Tomás Cervantes Cervantes Rincón, Tereza Frckova, Zaria I. Contejean, Jasmine Cantergiani, Kevin Groen, Benedetta Cena, Simone G. Moro, Filippo Bianchini, Luca Simonelli, David Jarrossay, et al.(Novus Biologicals, NBP2-35893), together with a live-cell Renilla luciferase substrate (EnduRen, Promega, E6481; 1:10,000), and incubated for 1 hour with constant shaking at 600 rpm. The serum:IFN-I mixtures were

(2) A non-spike nucleocapsid R204P mutation in SARS-CoV-2 Omicron XEC enhances inflammation and pathogenicityNature CommunicationsDecember 14, 2025Shuhei Tsujino, Masumi Tsuda, Sayaka Deguchi, Jumpei Ito, Taha Y. Taha, Hesham Nasser, Lei Wang, Julia Rosecrans, Rigel Suzuki, Saori Suzuki, et al.6-well plates. On day 4, target cells were incubated with EnduRen live cell substrate (Promega, Cat# E6481) for 3 h and then detached, and 32,000 target cells were added to a 96-well plate with effector cells.

(3) SERINC5 co-expressed with HIV-1 Env or present in a target membrane destabilizes small fusion pores leading to their collapsemBioDecember 10, 2025Ruben M. Markosyan, Mariana Marin, Gregory B. MelikyanProspect, IL, USA; B12200-0.05). EnduRen and Bright-Glo luciferase were from Promega (Madison, WI, USA; E6481), whereas BlaM substrate, CCF4-AM, was from Invitrogen (K1089). The Micro BCA Protein Assay Kit was

(4) Antigenic landscape of Nipah virus attachment glycoprotein analysis reveals a protective immunodominant epitope across speciesnpj VaccinesNovember 28, 2025Dan Zhou, Yong Wang, Yanfeng Yao, Wenhua Kuang, Rao Cheng, Gan Zhang, Hang Liu, Xin Li, Sandra Chiu, Zengqin Deng, et al.and replaced with fresh DMEM containing 10% FBS and 20 μM EnduRen live-cell substrate (Promega, Cat#: E6482). The cells were incubated for at least 2 h, and live-cell luciferase activity induced by glycoprotein-mediated

(5) Mutational dissection of HCMV gB and gH cytoplasmic tails highlights conserved and divergent features of fusion regulationmBioNovember 12, 2025Chanyoung Lee, Hannah K. DiBella, Ekaterina E. Heldweinwith 10% FBS, 1× Pen/Strep, 50 mM HEPES), containing 1:500 EnduRen live cell substrate (Promega, cat# E6482) added. After target cells were detached using 1 mL of Versene (Fisher Scientific, cat# 226-126) per

(6) Genome‐Wide CRISPR/Cas9 Screening Identifies the COMMANDER Recycling Complex as a Key Player in EV UptakeJournal of Extracellular VesiclesSeptember 23, 2025Miguel Palma‐Cobo, Victor Toribio, Joaquín Morales, Soraya López‐Martín, Carlos Enrich, Albert Lu, María Yáñez‐MóK562 or HeLa cells were resuspended in 300 µL of EV‐depleted medium with 60 µM of EnduRen (Promega, E6482) and left 2 h at 37°C in the incubator for reagent internalization and chemical deprotection by intracellular

(7) Monoclonal antibodies against human TMPRSS2 prevent infection by any SARS-CoV-2 variantiScienceSeptember 19, 2025Michishige Harada, Takehisa Matsumoto, Mizuki Yamamoto, Jin Goda, Akiko Idei, Kenichi Ohtaki, Natsuki Kojima, Natsumi Yoneda, Kosuke Miyauchi, Kazushige Katsura, et al.Kit,Qiagen,Cat#74136 TaqMan Fast Virus 1-step Master Mix,ThermoFisher,Cat#QPS-101T EnduRen Live cell Substrate,Promega,Cat#E6482 SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit,Sino Biological,Cat#KIT40588 ,, Deposited

(8) Proximity labelling of internalizing influenza A viruses reveals a role for neogenin in virus uptakePLoS pathogensJuly 1, 2025Sempere Borau M, Gisbert VG, von Kempis J, Arroyo-Fernández LM, Schiefer S, Alsteens D, Stertz S.and maintained in piDMEM supplemented only with 6µM EnduRen Live Cell Substrate (Promega, catalog# E6482) or also with 25mM NH4Cl (H1N1 in A549 cells) at 37°C. Luminescence was sampled at the indicated times

(9) Type I interferon autoantibody footprints reveal neutralizing mechanisms and allow inhibitory decoy designJ Exp MedJune 2, 2025Kevin Groen, Roger Kuratli, Jannik Enkelmann, Sonja Fernbach, Pedro D. Wendel-Garcia, Willy I. Staiger, Marylène Lejeune, Esther Sauras-Colón, Ferran Roche-Campo, Paraskevas Filippidis, et al.concentration of 0.2, 1, or 10 ng/ml, as well as the live-cell Renilla luciferase substrate (EnduRen, E6481; Promega; 1:10,000), and were incubated together for 1 h at room temperature with constant shaking at

(10) Split-Luciferase Reassembly Assay to Measure Endoplasmic Reticulum-Mitochondria Contacts in Live CellsJournal of Visualized ExperimentsOctober 11, 2024Connie Chen, Katherine A. Rafael, Ginam Cho, Youngshin Lim,ThermoFisher Scientific,14190144, EHop 016,Bio-Techne Tocris,6248,Dissolve in DMSO; store at −70 °C EnduRen Live Cell Substrate,Promega,E6481,Store aliquots at −70 °C Eppendorf 2-20 μL pipette,Eppendorf,3123000039, Eppendorf Research plus 100-1000

(11) A broadly protective antibody targeting glycoprotein Gn inhibits severe fever with thrombocytopenia syndrome virus infectionNature CommunicationsAugust 15, 2024Xuanxiu Ren, Jiawen Sun, Wenhua Kuang, Feiyang Yu, Bingjie Wang, Yong Wang, Wei Deng, Zhao Xu, Shangyu Yang, Hualin Wang, et al.cells. For live-cell luciferase activity measurements, 20 μM of EnduRen live-cell substrate (Promega, E6481) was diluted in DMEM and added to the cells. The cells were then incubated for at least 1 h before detection

(12) Potent neutralizing broad-spectrum antibody against SARS-CoV-2 generated from dual-antigen-specific B cells from convalescentsiScienceJune 16, 2023Takeshita M, Fukuyama H, Kamada K, Matsumoto T, Makino-Okamura C, Lin Q, Sakuma M, Kawahara E, Yamazaki I, Uchikubo-Kamo T, et al.Mix,New England Biolabs,Cat#N0447L KAPA HiFi HS ReadyMix,KAPA Biosystems,Cat#KK2602 EnduRen,Promega,Cat#E6481 20% formalin,Fujifilm Wako,Cat#064-03865 Cristal violet solution,Merck,Cat#V5265 Opti-MEM I,Thermo Fisher Scientific

(13) Virological characteristics of the SARS-CoV-2 XBB variant derived from recombination of two Omicron subvariantsNature communicationsMay 16, 2023Tamura T, Ito J, Uriu K, Zahradnik J, Kida I, Anraku Y, Nasser H, Shofa M, Oda Y, Lytras S, et al.(48 h posttransfection), target cells were incubated with EnduRen live cell substrate (Promega, Cat# E6481) for 3 h and then detached, and 32,000 target cells were added to a 96-well plate with effector cells.

(14) Convergent evolution of SARS-CoV-2 Omicron subvariants leading to the emergence of BQ.1.1 variantNature communicationsMay 11, 2023Ito J, Suzuki R, Uriu K, Itakura Y, Zahradnik J, Kimura KT, Deguchi S, Wang L, Lytras S, Tamura T, et al.(48 h posttransfection), target cells were incubated with EnduRen live cell substrate (Promega, Cat# E6481) for 3 h and then detached, and 32,000 target cells were added to a 96-well plate with effector cells.

(15) Analysis of SARS-CoV-2 variants B.1.617: host tropism, proteolytic activation, cell–cell fusion, and neutralization sensitivityEmerging Microbes & InfectionsDecember 31, 2022Li Zhang et al.The GFP signals were collected using BioTek Cytation 5V. The EnduRen live cell substrate (Promega, E6481, WI) and Ensight device (PerkinElmer) were used for luciferase activity detection. Structural modelling The

(16) Potent SARS-CoV-2 neutralizing antibodies with therapeutic effects in two animal modelsiScienceDecember 22, 2022Takeshita M, Fukuyama H, Kamada K, Matsumoto T, Makino-Okamura C, Uchikubo-Kamo T, Tomabechi Y, Hanada K, Moriyama S, Takahashi Y, et al.Mix,New England Biolabs,Cat#N0447L KAPA HiFi HS ReadyMix,KAPA Biosystems,Cat#KK2602 EnduRen,Promega,Cat#E6481 20% formalin,Fujifilm Wako,Cat#064-03865 Cristal violet solution,Merck,Cat#V5265 Opti-MEM I,Thermo Fisher Scientific

(17) The SARS-CoV-2 spike S375F mutation characterizes the Omicron BA.1 variantiScienceDecember 22, 2022Kimura I, Yamasoba D, Nasser H, Zahradnik J, Kosugi Y, Wu J, Nagata K, Uriu K, Tanaka YL, Ito J, et al.(48 h posttransfection), target cells were incubated with EnduRen live cell substrate (Promega, Cat# E6481) at 37°C for 3 h and then detached, and 32,000 target cells were added to a 96-well plate with effector

(18) Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assaySTAR protocolsDecember 16, 2022Nasser H, Shimizu R, Ito J, Genotype to Phenotype Japan (G2P-Japan) Consortium, Saito A, Sato K, Ikeda T.055-08975 G418,Wako,Cat# 070-06803 Trypsin/EDTA,Wako,Cat# 209-16941 EnduRen live cell substrate,Promega,Cat# E6481 Nonidet P40 substitute,Nacalai Tesque,Cat# 18558-54 Protease inhibitor cocktail,Nacalai Tesque,Cat#

(19) A second functional furin site in the SARS-CoV-2 spike proteinEmerging microbes & infectionsDecember 1, 2022Zhang Y, Zhang L, Wu J, Yu Y, Liu S, Li T, Li Q, Ding R, Wang H, Nie J, et al.after transfection, mixed at a 1:1 ratio and seeded into 96-well plates. EnduRen live cell substrate (E6481; Promega, Madison, WI) and an Ensight device (PerkinElmer) were used for luciferase activity detection. Neutralization assay

(20) Close relatives of MERS-CoV in bats use ACE2 as their functional receptorsNatureDecember 1, 2022Xiong Q, Cao L, Ma C, Tortorici MA, Liu C, Si J, Liu P, Gu M, Walls AC, Wang C, et al.Mshot). For measurements of live-cell luciferase activity, 20 μM of EnduRen live-cell substrate (Promega, E6481) was added to the cells in DMEM and incubated for at least 1 h before detection using the Varioskan

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