Lambda DNA-Hind III Digest from New England Biolabs

Product Specs
ItemLambda DNA-Hind III Digest
CompanyNew England Biolabs
Price $295.00 Inquire
Catalog NumberN3012L
Quantity750 gel lanes
Range2027-23130bps
Concentration500 µg/ml
ApplicationsDNA Manipulation
Nucleic Acid TypeLambda DNA
Features (standardized)Unstained
Size Range2027 - 23130 bp
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A Set of Novel Venom Proteins Enables Parasitoid Wasps to Exploit Older Hosts and Coexist with CompetitorsAdvanced ScienceJanuary 1, 2026Junwei Zhang, Zhi Dong, Yifeng Sheng, Jieyu Shan, Ting Feng, Wenqi Shi, Zixuan Xu, Zeying Wang, Qichao Zhang, Ying Wang, et al.cross‐linking reaction. The cross‐linked DNA was extracted and digested overnight with HindIII (NEB, Cat# N3012S). Biotin‐14‐dCTP17 was introduced during the sticky end repair process. The interacting DNA fragments

(2) Regeneration leads to global tissue rejuvenation in aging sexual planariansNature AgingApril 3, 2025Xiaoting Dai, Xinghua Li, Alexander Tyshkovskiy, Cassandra Zuckerman, Nan Cheng, Peter Lin, David Paris, Saad Qureshi, Leonid Kruglyak, Xiaoming Mao, et al.DNA was run on a 10-cm-long, 0.7% agarose-1× TAE gel, along with a λ DNA-HindIII digest ladder (NEB; N3012S) at a constant voltage of 70 V for 3 h. The gel was denatured in 0.5 M NaOH for 20 min at room temperature

(3) A clinically annotated post-mortem approach to study multi-organ somatic mutational clonality in normal tissuesScientific ReportsDecember 1, 2022Tom Luijts et al.(Thermo Fisher Scientific). DNA integrity was determined by agarose gel electrophoresis, with Hind III Digested lamda DNA (N3012S, NEB) loaded as a molecular marker. Deep targeted sequencing A gene panel of 153 genes was developed

(4) An Enzyme-Linked Immunosorbent Assay for the Detection of Mitochondrial DNA-Protein Cross-Links from Mammalian CellsDNA (Basel)December 1, 2022Xu W, Zhao L.The Monarch™ genomic DNA purification kit (T3010), Monarch™ PCR & DNA cleanup kit (T1030), and λ DNA (N3012) were from New England Biolabs. Turbonuclease (T4330) was from Sigma-Aldrich (St. Louis, MO, USA). HEK293

(5) Primary acute lymphoblastic leukemia cells are susceptible to microtubule depolymerization in G1 and M phases through distinct cell death pathwaysThe Journal of biological chemistryJune 1, 2022Delgado M, Rainwater RR, Heflin B, Urbaniak A, Butler K, Davidson M, Protacio RM, Baldini G, Edwards A, Reed MR, et al.an initial switch time of 1.5 s and a final switch time of 3.5 s for 12 h at 6 V/cm. Lambda HindIII (N3012S, New England Biolabs) was used to provide molecular mass standards. DNA was visualized by staining gels

(6) Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissuesProceedings of the National Academy of SciencesMay 25, 2021Liguo Zhang, Alexsia Richards, M. Inmaculada Barrasa, Stephen H. Hughes, Richard A. Young, Rudolf Jaenischa 0.4% (wt/vol) agarose/1× TAE gel for 1.5 h with a 3 V/cm voltage, with λ DNA-HindIII Digest (NEB; N3012S) as size markers. Large fragments (>23.13 kb) were cut out, frozen in −80 °C, and then crushed with

(7) Comparative Anaerobic Decolorization of Azo Dyes by Carbon-Based Membrane BioreactorWaterApril 12, 2021Mohammad Shaiful Alam Amin, Frank Stüber, Jaume Giralt, Agustin Fortuny, Azael Fabregat, José FontCorporation, ref. 64000, Thorold, ON, Canada), λ DNA/HindIII Markers (New England Biolab Inc. ref. N3012S, Ipswich, MA, USA), Agarose (Sigma Aldrich, ref. 1.16801, Spain), TBE buffer (Sigma Aldrich, ref. 574796, Water

(8) Plasmid replication-associated single-strand-specific methyltransferasesNucleic Acids ResearchDecember 16, 2020Alexey Fomenkov, Zhiyi Sun, Iain A Murray, Cristian Ruse, Colleen McClung, Yoshiharu Yamaichi, Elisabeth A Raleigh, Richard J Robertsvisualized with ECL Hyperfilm™ (Amersham, NJ). DNA markers for fluorography were made by modification of NEB N3012 (Lambda HindIII digest) or NEB N3233 (Low Molecular Weight DNA Ladder) with the nonspecific A-DNA MTase,

(9) CMG helicase disassembly is controlled by replication fork DNA, replisome components and a ubiquitin thresholdeLifeAugust 17, 2020Tom D Deegan, Progya P Mukherjee, Ryo Fujisawa, Cristian Polo Rivera, Karim Labibfor in vitro DNA replication reactions Recombinant DNA reagent,λ HindIII Digest,New England Biolabs,N3012S,Molecular weight marker for agarose gels Sequence-based reagent,6664,This study,N/A,CDC34 forward primer

(10) The CURE for Cultivating Fastidious Microbes†Journal of Microbiology & Biology EducationApril 26, 2019Arundhati Bakshi, Austen T. Webber, Lorelei E. Patrick, William Wischusen, Cameron Thrashthe amplicon from isolate DNA (expected size 1,466 bp); “Ladder” is Lambda HindIII digest ladder (NEB N3012S), with the lowest visible band at 2,027 bp; “Control” is the negative control (water).

(11) Pif1-Family Helicases Support Fork Convergence during DNA Replication Termination in EukaryotesMolecular CellApril 18, 2019Tom D. Deegan, Jonathan Baxter, María Ángeles Ortiz Bazán, Joseph T.P. Yeeles, Karim P.M. Labibmolecular weight markers were prepared by dephosphorylating 17 μg λ DNA-HindIII Digest (New England Biolabs N3012S) with 10 U Antarctic Phosphatase (New England Biolabs M0289S) in total volume of 40 μL for 1 h at 37°C.

(12) The Initial Response of a Eukaryotic Replisome to DNA DamageMolecular CellJune 21, 2018Martin R.G. Taylor, Joseph T.P. Yeeles,New England Biolabs,Cat# N4040S λ DNA-HindIII Digest: molecular weight markers,New England Biolabs,Cat# N3012S pJM126 (RPA purification),Addgene,#49339 pAM3 (Cdc6 purification),"Frigola et al., 2013",N/A vJY19

(13) MitoNeoD: A Mitochondria-Targeted Superoxide ProbeCell Chemical BiologyOctober 19, 2017Maria M. Shchepinova et al.CAS:146819-28-8 Menadione,Sigma-Aldrich,Cat#M5625-25G; CAS: 58-27-5 λ DNA HindIII Digest,NEB,Cat#N3012S φ X174 DNA-HaeIII Digest,NEB,Cat#N3026S Salmon sperm DNA,Sigma-Aldrich,Cat#D1626-1G; CAS: 438545-06-3 zirconium

(14) Why Johnny can't clone: Common pitfalls and not so common solutionsBioTechniquesSeptember 1, 2015Ichiro Matsumurabe tested using a positive control; for instance, the commercially available l HindIII digest (e.g., N3012S, New England Biolabs), which is more often employed as a molecular weight standard for agarose gel electrophoresis

(15) Genome-wide linkage analysis of quantitative biomarker traits of osteoarthritis in a large, multigenerational extended familyArthritis & RheumatismMay 9, 2013Hsiang-Cheng Chen et al.one hour) using 0.5 µl aliquots of each sample. A HindIII digest of lambda DNA (New England Biolabs, N3012S) was used as a reference ladder. DNA was scored 0 – 5 with score ≥4 indicating that a single high molecular

(16) Inhibitors of Streptococcus pneumoniae Surface Endonuclease EndA Discovered by High-Throughput Screening Using a PicoGreen Fluorescence AssayJ Biomol ScreenMarch 1, 2013Eliza J.R. Peterson, Dmitri Kireev, Andrea F. Moon, Marika Midon, William P. Janzen, Alfred Pingoud, Lars C. Pedersen, Scott F. Singletonfrom Molecular Probes by Life Technologies (Grand Island, NY). Lambda DNA-Hind III digest (Cat. No. N3012L) was purchased from New England Biolabs (Ipswich, MA). The cloning, production, and purification of

(17) Rapid bacterial artificial chromosome modification for large-scale mouse transgenesisNature ProtocolsOctober 2, 2010Shiaoching Gong, Laura Kus, Nathaniel Heintzendonuclease (New England Biolabs, cat. no. R0696S) λ-DNA-HindIII Digest (New England Biolabs, cat. no. N3012S) Low-range PFG marker DNA ladder (New England Biolabs, cat. no. NO350S) 2-log DNA ladder (New England Biolabs

(18) Continuous-flow bioseparation using microfabricated anisotropic nanofluidic sieving structuresNature ProtocolsNovember 1, 2009Jianping Fu, Pan Mao, Jongyoon HanTris-Borate-EDTA (TBE) powder (Cat. NO. T7527, Sigma-Aldrich, St. Louis, MO) λ DNA–Hind III digest (Cat. NO. N3012L, New England BioLabs, Beverly, MA) YOYO-1 dye (Cat. NO. Y3601, Molecular Probes, Eugene, OR) Alexa

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