Klenow Fragment (35 exo-) from New England Biolabs

Product Specs
ItemKlenow Fragment (35 exo-)
CompanyNew England Biolabs
Price $271.00 Inquire
Catalog NumberM0212L
Quantity1000 units
Concentration5,000 units/ml
ApplicationsDNA Manipulation
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to convert 10 nmol of dNTPs to an acid-insoluble material in 30 minutes at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) SPACE-seq integrates spatial transcriptomics and lineage tracing in native tissuesCell Stem CellAugust 6, 2026Yuemeng Jia, Dawei Sun, Jackson A. Weir, Xugeng Liu, Andrew J. C. Russell, YeEun Kim, Nicholas Thom, Giovanni Marrero, Vipin Kumar, Fei Chen, et al.Reverse Transcriptase,Thermo Fisher Scientific,Cat #EP0751 Klenow Fragment,New England Biolabs,Cat #M0212 Terra PCR Direct Polymerase,Takara,Cat #639270 SPRIselect Beads,Beckman Coulter,Cat #B23318 BioAnalyzer

(2) CTCF delimits a DNA methylation transition at the intergenic region between the embryonic and adult α-globin genesEpigeneticsAugust 3, 2026Gustavo Tapia-Urzúa, Hober Nelson Núñez-Martínez, Sylvia Garza-Manero, Martin Franke, Carlos Alberto Peralta-Alvarez, Ángel Josué Cerecedo-Castillo, Georgina Guerrero, José Luis Gómez-Skarmeta, Félix Recillas-TargaKlenow [NEB, M0210]), A-tailing mix (1X NEB buffer 2, 0.5 mM dATP, and 0.25 U/µL Klenow, exo- [NEB, M0212]), and CIP (NEB, M0290). The samples were indexed by ligating TruSeq Illumina adapters by incubating

(3) Protocol for in vivo DNA-RNA hybrid immunoprecipitation, sequencing, and analysis from frozen mouse tissuesSTAR ProtocJuly 23, 2026Hassan Massalha, Cameron J. Chee, Julia S.P. Mawer, Francesco Puzzo, Magdalena P. CrossleyRNase H,NEB,M0297S Glycogen,Thermo Fisher,R0561 NEBNext® End Repair Module,NEB,E6050S Klenow Fragment (3'→5' exo-),NEB,M0212S IDT xGen™ Stubby Adapter and UDI Primers (Alternative: xGen Stubby Adapters can be purchased separately

(4) Genome-wide rotational and translational phasing of nucleosomes with human transcription factorsMol CellJuly 16, 2026Haining Chen, Jordan E. Krebs, Olivia W. Lang, Jiayi Hu, Devin C. Mellini, Judith Hyle, Chunliang Li, William K. M. Lai, B. Franklin Pugh11119915001 "Proteinase K, 20mg/ml",Thermo Scientific,cat.no. EO0491 Klenow exo minus,New England BioLabs,cat.no. M0212S T4 DNA Polymerase,New England BioLabs,cat.no. M0203S Lambda exonuclease,New England BioLabs,cat.no.

(5) Impacts of DNA methylation on H2A.Z deposition and nucleosome stabilityElifeJuly 7, 2026Rochelle M Shih, Yasuhiro Arimura, Hide A Konishi, Hironori Funabikibiotin-14-dATP (Jena Bioscience, Cat# NU-835-BIO14-S) using Klenow Fragment (3′→ 5′ exo-) (NEB Cat# M0212) following manufacturer’s protocol. Biotinylated DNA was phenol–chloroform extracted and bound to M-280

(6) Defined bacterial consortium highlights the impact of intestinal bacteria on DNA methylation and tumorigenesisGenome BiolJuly 7, 2026Claudia Mercado-Rodriguez, Siddhi Chitre, Pyoung Hwa Park, Ye Yang, Anthony Pompetti, Raad Z. Gharaibeh, Jason O. Brant, Jean-Pierre J. Issa, Christian Jobinendonuclease (NEB, #R0106L) at 37 °C for 4 h. Blunt ends were created using Klenow DNA polymerase (NEB, #M0212) by filling in 5’ overhangs and generate 3’ dA overhangs. The size of the fragments was determined by

(7) Distinct associations of pioneer factor Ascl1-E12a with nucleosomes drive changes in cell fateMolecular CellJuly 2, 2026Bing-Rui Zhou, Edgar Luzete-Monteiro, Jingchao Zhang, Naomi Takenaka, Hsin-Yao Tang, Meilin Fernandez Fernandez Garcia, Mariel Coradin, Megan Frederick, Greg Donahue, Benjamin Garcia, et al.#2, 4mM dATP, 4mM dTTP, 4mM dGTP, 4uM Cy5-dCTP (Cytiva PA55021), 0.5U/uL Klenow fragment -exo (NEB M0212), and incubating the reaction at 37°C for 3h. The labeled DNA was then purified using QIAquick PCR purification

(8) Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effectsCellJune 25, 2026Sriram Pendyala, Katie Partington, Nicholas Bradley, Abbye E. McEwen, Gwenneth Straub, Hyeon-Jin Kim, Shawn Fayer, Daniel Lee Holmes, Katherine A. Sitko, Riddhiman K. Garge, et al.PBv2b-CSapI, and PBv2c-NSapI) were barcoded using Golden Gate assembly. Klenow fill-in (New England Biolabs, M0212L) of VISseq_GG_BColigo (IDT) using primer VS_01 (IDT) yielded a double-stranded oligo with a 12-bp degenerate

(9) DNA repair drives cisplatin-induced neuronal deathCellJune 25, 2026William J. Nathan, Chuanyuan Chen, Rosy Sakr, Bruno Siqueira Siqueira Mietto, Vincent van Batenburg, Jeroen van den Berg, Josette J. Wlaschin, Ferenc Livak, Elsa Callen, Nancy Wong, et al.twice in NEBNext dA-Tailing Reaction Buffer (NEB, B6059S) and then incubated with Klenow exo- (NEB, M0212S) in dA-Tailing Reaction Buffer at 37°C for 30 minutes. Plugs were washed once in NEBuffer 2 and incubated

(10) Correlated protein-RNA associations and a requirement for HNRNPU in the long-range recruitment of Polycomb Repressive Complexes by the lncRNAs Airn and Kcnq1ot1PLOS GeneticsJune 23, 2026McKenzie M. Murvin, Shuang Li, Elizabeth W. Abrash, Bridget A. Peck, Samuel P. Boyson, Zhiyue Zhang, Rachel E. Cherney, J. Mauro CalabreseNEBNext End Repair Module (NEB, cat #: E6050S), A-tailing by Klenow Fragment (3’/50 exo-; NEB, cat #: M0212S), and TruSeq 6-bp index adaptor ligation by Quick ligase (NEB, cat #: M2200S), and NEBNext High-Fidelity 2X PCR Master Mix

(11) Quantitative profiling of RNA modifications enriched in non-membrane-bound cellular structures using APEX-RNA-MSCell Chemical BiologyJune 22, 2026Kyung W. Seo, Dhruv Y. Dhingani, Ralph E. Kleinerfor tRNA synthesis were generated through primer extension using Klenow Fragment (3’ → 5’ exo-) (NEB, M0212S). In vitro transcription (IVT) was done using HiScribe T7 High Yield RNA Synthesis kit (NEB, E2040S)

(12) Longitudinal changes in DNA methylation in IDH-mutant glioma fuel disease progression through altered cell state differentiationNature GeneticsJune 22, 2026Masashi Nomura, Ramya Raviram, Joshua S. Schiffman, Lillian Bussema, Vivian Lu, Noelle Wheeler, John J. Y. Lee, Yilin Fan, Mian Hua Zheng, Florian Ruiz, et al.°C. Digested DNA was filled in and A-tailed at the 3′ sticky ends with 2.5 U of Klenow fragment (NEB, M0212L). The reaction was supplemented with 2 mM dATP, 0.2 mM dCTP and 0.2 mM dGTP (Thermo, R0193) and performed

(13) SmartTrap: automated precision experiments with optical tweezersNature MethodsJune 18, 2026Martin Selin, Antonio Ciarlo, Giuseppe Pesce, Lars Bengtsson, Joan Camunas-Soler, Vinoth Sundar Rajan, Fredrik Westerlund, L. Marcus Wilhelmsson, Isabel Pastor, Felix Ritort, et al.\setlength{\oddsidemargin}{-69pt} \begin{document}$${3}^{{\prime} }- > {5}^{{\prime} }$$\end{document}3′−>5′ exo-),NEB,M0212S "Quick ligation kit, 30 reactions",NEB,M2200S Taq DNA polymerase,NEB,M0273S Antarctic phosphatase,NEB,M0289S "Biotin-14-dCTP,

(14) Skin-derived G-CSF activates pathological granulopoiesis upon psoriasisEMBO Molecular MedicineJune 16, 2026Tomson Kosasih, Tatsuya Morishima, Sohyeon Lee, Jungyeon Yoon, Kanako Wakahashi, Pilhan Kim, Aiko Sada, Hitoshi TakizawaOligo(dT) 18 primer,Thermo,Cat# SO131 1st NSR primer mouse,SIGMA, 2nd NSR primer mouse,SIGMA, NEB buffer 2,NEB,Cat# M0212L Klenow fragment (3’-5’ exo-),NEB,Cat# M0212L dNTPs,Takara,Cat# 4030 AMPure XP beads,Beckman Coulter,Cat#

(15) Improved short nascent strand sequencing (iSNS-seq) enhances DNA replication origin detection and reduces non-origin biasesbioRxivMay 30, 2026Miiko Sokka, John M. Urban, Nicola Neretti, Susan A. Gerbiand 5 U of DNA Polymerase I Large (Klenow) fragment (New England Biolabs, Ipswich, MA, catalog Nr. M0212S) in 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2, 1 mM DTT, 100 μM dNTP. The polymerase was inactivated

(16) Lamin dosage in cardiomyocytes supports nuclear organization and chromatin gating for transcriptionbioRxivMay 26, 2026Katherine A. Bossone, Xiaobin Zheng, Lidya Kristiani, Reni Marsela, Youngjo Kim, Yixian Zheng(NEB, B7002S), 5.0 μL of 1 mM dATP (NEB, N0440S), and 1.5 μL of Klenow fragment (3'→5' exo-) (NEB, M0212L; 5 units/μL), followed by incubation at 37°C for 30 minutes without agitation in a thermal cycler. Beads

(17) Polycomb chromatin topology enables long-range enhancer recruitment during craniofacial developmentNature CommunicationsMay 14, 2026Yousra Ben Zouari, Onkar Joshi, Adwait Salvi, Sandra Kessler, Sebastien Ducret, Fiona Ross, Sjoerd J. B. Holwerda, Nathalie Vilain, Soujanya Mamilla-Sanivaram, Sebastien Smallwood, et al.mixed with 50 µL 10× NEBuffer 2, 5 µL 10 mM dATG and 20 µL 5 U/µL Klenow Fragment (3’ → 5’ exo-) (NEB, M0212), and then incubated at 37 °C for 30 min. Beads were reclaimed on a magnetic separation stand, supernatant

(18) IL-6 receptor blockade impedes proinflammatory atypical Treg subset associated with immune checkpoint inhibitor–induced inflammatory arthritisJ Clin InvestMay 12, 2026Yifei Ma, Nianqi Liu, Yan Li, Denghan Zhang, Shaohui He, Jun Lv, Yongluo Jiang, Guangmin Jian, Jingyao Zhang, Pengfei Zhu, et al.events. Nucleotides were denatured, hybridized, and went through Klenow extension (New England Biolabs, #M0212L) and treatment with Exonuclease. cDNAs were prepared in nested PCR reaction, followed by index PCR with

(19) Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in miceMetabolism: clinical and experimentalMay 1, 2026So J, Wann J, Kim K, Taleb S, Kim HG, Kumari M, Banks AS, Dong XC, Roh HC.the End-It DNA End-Repair Kit (Epicenter, ER0720), followed by A-tailing with Klenow Fragment (NEB, M0212) and adaptor ligation with Quick Ligase (NEB, M2200). Libraries were then PCR-amplified for 18 cycles

(20) Checkpoint blockade-related CNS adverse events are characterized by neurotoxic T helper cells with sustained neuroantigen reactivityCell Rep MedApril 21, 2026Yifei Ma, Ao Zhang, Jiacai Lin, Fadian Ding, Xinjia Wang, Weidong Wang, Guangmin Jian, Jingyao Zhang, Pengfei Zhu, Yue Ma, et al.events. Nucleotides were denatured, hybridized and went through Klenow extension (New England Biolabs, #M0212L) and treatment with Exonuclease. CDNAs were prepared in nested PCR reaction, followed by index PCR with

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