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Li-Cor’s Odyssey Infrared Imaging System
January 21, 2004
The Li-Cor Odyssey Infrared Imaging System yields reproducible results, flexibilty on scanning the membrane, and easy ... read more
MetaPhor Agarose from Lonza
December 17, 2003
For more than five years now, I have used MetaPhor agarose to help me genotype my transgenic mice... read more
Spectra/Por® Dialysis Tubing by Spectrum® Laboratories Inc.
December 10, 2003
I have recently been generating many fluorescent analogs of proteins and have used dialysis to remove ammonium sulfate from ... read more
CLONTECH’s NucleoSpinPlus Plasmid Miniprep Kit
December 03, 2003
The Clontech NeucleoSpin Plus Plasmid Miniprep Kit is used in our laboratory to purify high quality plasmid DNA for a variety of ... read more
Novagen's BugBuster GST-Bind Kit
November 19, 2003
Recently, I needed to express and purify GST-fusion proteins for antibody production. Since neither I nor anyone else in the lab ... read more
Invitrogen’s TA Cloning Kit (Dual Promoter)
November 06, 2003
The TA cloning kit from Invitrogen proves a highly efficient method of cloning a PCR product into a plasmid vector in one step. ... read more
Thermo Electron Corporation's Px2 Thermocyclers
October 22, 2003
Gradient thermocyclers greatly simplify optimization of traditional, touchdown and step-down PCR. The confusing part is deciding ... read more
Premium RNA (Clontech, a Takara Bio Company)
October 15, 2003
Clontech now has the largest available selection of premium quality Total and poly A+ RNAs of any vendor. Potential applications ... read more
GE Healthcare's NAP-25 Column
October 08, 2003
Recently, our lab began using alpha-P32 dCTP-labeled oligonucleotides to screen a BAC library (actually a high-density nylon ... read more
The GeneGenius Gel Imaging System from Syngene
October 01, 2003
The GeneGenius Imaging System from Syngene is designed for the detection and documentation of nucleic acids and proteins in gels. ... read more
The EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit from Pierce Biotechnology
September 17, 2003
Enzyme-linked immunosorbent assays (ELISAs) that are intended to quantitate peptides or proteins in biological fluids must be very... read more
Apoalert Mitochondrial Membrane Sensor Kit (Clontech, a Takara Bio Company)
September 10, 2003
Apoptosis, or programmed cell death, plays an important role in many developmental processes. The detection of apoptotic cells, ... read more
R&D Systems' cAMP ELISA Kit
September 03, 2003
Then I switched over to the cAMP Assay Kit by R&D Systems. The biggest advantage of this kit is that it is non radioactive, which ... read more
Molecular Probes' Alexa Fluor 488 Signal-Amplification Kit for Mouse Antibodies
August 27, 2003
Recently, our lab has been performing immunoflourescence studies using mouse monoclonal antibodies to detect various antigens in ... read more
The Milli-RX Water Purification System from Millipore
August 20, 2003
The Milli-RX system from Millipore is a heavy duty water purification system for the lab. The Milli-RX system uses ordinary tap ... read more

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Detection of LAP+ Treg Cells by Flow Cytometry

Mitesh Dwivedi

The aim of the experiment was to detect LAP+ CD4+CD25+ regulatory T cells (Tregs) isolated from whole blood of humans. read more

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