Inside Stain Kit from Miltenyi Biotec

(1) Comprehensive In Vitro Profiling Demonstrates Successful Immune Evasion of Bioengineered Immune‐Shielded Heart ValvesAdvanced Healthcare MaterialsOctober 1, 2026Abraham van van Wijk, Leanne Van den den Berg, Isa B. M. Velraeds, Valentina Osorio Zuluaga, Emma T. M. Peereboom, Martijn J. W. E. Rabelink, Arnaud Zaldumbide, Rob C. Hoeben, Marie‐Jose T. H. Goumans, Eric Spierings, et al.Dissociation Kit (Miltenyi Biotec #130‐110‐204) and fixed using the Inside Stain Kit (Miltenyi Biotec #130‐090‐477). Antibodies used were: Granzyme B‐PE‐Dazzle (1:100, biolegend #372216), CD8‐605 (1:100, biolegend #

(2) Exercise-Mobilized Donor Lymphocytes Enhance Blinatumomab Activity Against B-ALLResearch SquareAugust 13, 2026John L Sullivan, Geovana Leite, Nina Santa Cruz, Helena Batatinha, Kaitlin A Swanson, Dimitrios Filioglou, Grace M McKenzie, Timothy M Kistner, Prabhu S Arunachalam, Forrest L Baker, et al.delineated by CD56 and CD3 expression. Intracellular NKG7 staining used the Miltenyi Inside Stain Kit (130-090-477) with unconjugated anti-NKG7 (Thermofisher) and Goat anti-Rabbit FITC secondary antibody. Additional

(3) TGF-β signalling drives chemotaxis of human induced pluripotent stem cell-derived cardiomyocytes in response to MI stimulusBasic Research in CardiologyAugust 12, 2026Laura Deelen, Kazuya Kobayashi, Gowtham Reddy Cheruku, Alia Hafiz Abbas Gasim, Fiona Lewis-McDougall, Ken SuzukiCardiomyocyte Dissociation Kit (see above) and stained with the Inside Stain Kit (Miltenyi Biotech, 130-090-477) according to the manufacturer’s protocol. Briefly, dissociated cells are counted with Trypan Blue and

(4) Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firingStem Cell Res TherJuly 7, 2026Oliver Dräger, Angelique Grell, Asaria E. Vogel, Erhard Wischmeyer, Beatrice A. Nossek200xg for 5 min. Subsequently, cells were incubated in inside fix solution of the Inside Stain Kit (#130-090-477, Miltenyi Biotec, Bergisch Gladbach, Germany) for 20 min at RT and centrifuged at 200xg for 5 min. After

(5) A Workflow for Assessing Antibody-Drug Conjugate Target Expression on Circulating Tumour Cells from Triple-Negative Breast Cancer and Epithelial Ovarian Cancer PatientsThe Journal of Liquid BiopsyJune 30, 2026Brian D. Henderson, Lauren McMahon, Faye Lewis, Caroline Marion, Sinead Hurley, Kathy Gately, Irene Narinda, Marika Kanjuga, Lucy Norris, Cara Martin, et al.All antibodies were diluted 1:120 in Inside Perm (Inside Stain Kit, MACS Miltenyi Biotec, Germany; #130-090-477). Ovarian cells were stained with an antibody cocktail for positive selection: anti-EpCAM (9C4, Alexa

(6) Therapeutic delivery of albumin-binding siRNA targeting IRS2 to diverse cell types reduces mammary tumor growthMolecular TherapyJanuary 29, 2026Claire E. Tocheny, Julianna E. Buchwald, Christopher D. Dahlke, Hassan H. Fakih, Jennifer S. Morgan, Ashley Summers, Christi A. Silva, Samuel O. Jackson, Ji-Sun Lee, Michael-Anthony Card, et al.Viability Dye (Invitrogen #65–0865-14) and fixed using a formaldehyde-based fixative (Miltenyi Cat# 130–090-477). Samples were run on a Cytek Aurora flow cytometer and analyzed using FlowJo v10 software. Single-stained

(7) Simultaneous secretion of a PD-L1 x 4-1BB bispecific antibody improves antileukemic efficacy of STAb-T cells secreting a CD19-specific T-cell engagerOncoimmunologyDecember 31, 2025Marina Gómez-Rosel, Laura Rubio-Pérez, Ivana Zagorac, Carmen Domínguez-Alonso, Laura Díez-Alonso, Oana Hangiu, Rocío Navarro, Raúl Torres-Ruíz, Sandra Rodríguez-Perales, Francisco J. Blanco, et al.APC-conjugated anti-His mAb. Intracellular bsAbs were detected using the Inside Stain Kit (Cat. No. 130−090−477; Miltenyi Biotec), following the manufacturer’s instructions, and an anti-His APC mAb. Alternatively,

(8) Loss of ADAM15 prevents necroptosis induction by partial RIPK1 degradation due to enhanced TNF-R1 surface expression and basal caspase-8 activationCell Commun SignalDecember 4, 2025S. Braun, K. Knackfuß, T. Ziesmann, L. Mlinzk, A. Goerg, J. Frankenheim, A. Walter, W. Schneider-Brachert, U. Distler, J. Fritschlocated both inside cells and on the cell surface [46], we utilized the inside stain kit and protocol (130–090–477, Miltenyi). 1 × 106 cells were sedimented, washed with PBS (10 min, 300 × g), and resuspended in 250

(9) Simultaneous secretion of a PD-L1 x 4-1BB bispecific antibody improves antileukemic efficacy of STAb-T cells secreting a CD19-specific T-cell engagerOncoimmunologyOctober 26, 2025Marina Gómez-Rosel, Laura Rubio-Pérez, Ivana Zagorac, Carmen Domínguez-Alonso, Laura Díez-Alonso, Oana Hangiu, Rocío Navarro, Raúl Torres-Ruíz, Sandra Rodríguez-Perales, Francisco J. Blanco, et al.APC-conjugated anti-His mAb. Intracellular bsAbs were detected using the Inside Stain Kit (Cat. No. 130−090−477; Miltenyi Biotec), following the manufacturer’s instructions, and an anti-His APC mAb. Alternatively,

(10) Circulating tumour cells are a prognostic indicator in advanced high-grade serous ovarian cancer and are associated with platelets and immune cells following disseminationBritish Journal of CancerOctober 10, 2025Mark P. Ward, Faye Lewis, Catherine O’Gorman, Lucy A. Norris, Sarah E. Lochrin, Laura E. Kane, Tanya E. Kelly, Bashir M. Mohamed, Ashitha Ramesh, Roisin O’Connor, et al.paraformaldehyde (Sigma Aldrich, Ireland) followed by incubation with permeabilization buffer Inside Perm (Cat:130-090-477, Inside stain Kit; Miltenyi Biotec, Germany). For metastatic breast cancer patient samples, slides were

(11) Myocardial disarray drives metabolic inefficiency in human cardiomyocytesCommunications biologyOctober 9, 2025Jouve C, Ruiz-Velasco A, Donnarumma E, Le Borgne R, Momken I, Pereira C, Seguret M, Vermersch E, Vimont E, Nemazanyy I, et al.manufacturer’s protocol. Then, collected cell pellets were fixed and permeabilized using Inside Stain kit (130-090-477 Miltenyi Biotech) for 10 min at room temperature. Cells were incubated with fluorescent labeled antibodies

(12) GMP-Compliant Process for the Manufacturing of an Extracellular Vesicles-Enriched Secretome Product Derived From Cardiovascular Progenitor Cells Suitable for a Phase I Clinical TrialJ Extracell VesiclesAugust 20, 2025Camille Humbert, Chloé Cordier, Iouri Drut, Michele Hamrick, Jacquelyn Wong, Valérie Bellamy, Justine Flaire, Kiranmayee Bakshy, Florent Dingli, Damarys Loew, et al.manufacturer's instructions. Intracellular staining was performed using Inside stain kit (Miltenyi Biotec, #130‐090‐477) according to the manufacturer's instructions. Flow cytometric analyses were performed on day+3 and

(13) Frequency of synaptic antigen-specific CD4+ T cells in dementia is age-dependent but not correlated with cognitive impairmentImmun AgeingJune 19, 2025Julius Hoffmann, Marie-Luise Machule, Jakob Kreye, Laura Stöffler, Péter Körtvelyessy, Maria Buthut, Rosa Rößling, Petra Bacher, Alexander Scheffold, Harald PrüssMiltenyi, 130–109–812). Following cell permeabilization and fixation (Miltenyi inside stain kit, Miltenyi 130–090–477), intracellular epitopes were stained with antibodies targeting CD154 (PE, Miltenyi, 5 C8, 130–113–607,

(14) Distinct HLA Haplotypes Are Associated With an Altered Strength of SARS-CoV-2-Specific T-Cell Responses and Unfavorable Disease CoursesEur J ImmunolApril 21, 2025C. Dörnte, A. Datsi, V. Traska, J. Kostyra, M. Wagner, O. Brauns, C. Lamsfuß, H. Winkels, V. Balz, J. Enczmann, et al.markers, cell fixation was performed using Inside Fix (Inside Stain Kit, Miltenyi Biotec, cat. no. 130‐090‐477), according to the manufacturer's instruction, followed by resuspension in 250 µL/well Inside Perm (Inside Stain Kit

(15) Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cellsStem Cell ReportsOctober 8, 2024Zeina R. Al Sayed, Charlène Jouve, Magali Seguret, Andrea Ruiz-Velasco, Céline Pereira, David-Alexandre Trégouët, Jean-Sébastien HulotBioLegend). The collected cell pellets were then fixed and permeabilized with the Inside Stain kit (130-090-477, Miltenyi Biotec) for 10 min at room temperature. Cells were incubated with either Allophycocyanin (APC)

(16) A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancerMolecular OncologyAugust 6, 2024Volga M Saini, Ezgi Oner, Mark P. Ward, Sinead Hurley, Brian David Henderson, Faye Lewis, Stephen P. Finn, Gerard J. Fitzmaurice, John J. O'Leary, Sharon O'Toole, et al.antibody [V9, Alexa Fluor 546, sc‐6260]) were added to 399 μL of permeabilization buffer Inside Perm (#130‐090‐477, Inside stain Kit; Miltenyi Biotec, Bergisch Gladbach, Germany), along with 3.5 μL of 1 mg·mL−1 Hoechst

(17) A versatile high-throughput assay based on 3D ring-shaped cardiac tissues generated from human induced pluripotent stem cell-derived cardiomyocyteseLifeApril 5, 2024Magali Seguret, Patricia Davidson, Stijn Robben, Charlène Jouve, Celine Pereira, Quitterie Lelong, Lucille Deshayes, Cyril Cerveau, Maël Le Berre, Rita S Rodrigues Ribeiro, et al.Then, collected cell pellets were fixed and permeabilized using Inside Stain kit (Miltenyi Biotech, 130-090-477) at room temperature for 10 min. Cells were incubated with either APC anti-cardiac troponin T (CTNT)

(18) A versatile high-throughput assay based on 3D ring-shaped cardiac tissues generated from human induced pluripotent stem cell-derived cardiomyocytesElifeApril 5, 2024Magali Seguret, Patricia Davidson, Stijn Robben, Charlène Jouve, Celine Pereira, Quitterie Lelong, Lucille Deshayes, Cyril Cerveau, Maël Le Berre, Rita S Rodrigues Ribeiro, et al.Then, collected cell pellets were fixed and permeabilized using Inside Stain kit (Miltenyi Biotech, 130-090-477) at room temperature for 10 min. Cells were incubated with either APC anti-cardiac troponin T (CTNT)

(19) Nicotinamide-Expanded Allogeneic Natural Killer Cells with CD38 Deletion, Expressing an Enhanced CD38 Chimeric Antigen Receptor, Target Multiple Myeloma CellsInt J Mol SciDecember 7, 2023Avishay Edri, Nimrod Ben-Haim, Astar Hailu, Nurit Brycman, Orit Berhani-Zipori, Julia Rifman, Sherri Cohen, Dima Yackoubov, Michael Rosenberg, Ronit Simantov, et al.fixation and permeabilization using an Inside Stain Kit (Miltenyi, Bergisch Gladbach, Germany. cat#130-090-477) according to the manufacturer’s instructions. After permeabilization, intracellular staining was performed

(20) Inhibition of microfold cells ameliorates early pathological phenotypes by modulating microglial functions in Alzheimer's disease mouse modelJournal of neuroinflammationNovember 27, 2023Kim N, Ju IG, Jeon SH, Lee Y, Jung MJ, Gee MS, Cho JS, Inn KS, Garrett-Sinha LA, Oh MS, et al.anti-CD68 Alexa488 (1:50, BioLegend, 137012) were performed with Inside Stain kit (Miltenyi Biotec, 130-090-477) according to the manufacturer’s instructions. After an incubation period of 1 h at 4 °C, fluorescence

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Inside Stain Kit

Inside Stain Kit from Miltenyi Biotec

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Inside Stain Kit from Miltenyi Biotec

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