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Intracellular Flow Cytometry Staining Kits
Inside Stain Kit
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Inside Stain Kit
Inside Stain Kit
Inside Stain Kit from Miltenyi Biotec
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Citations:
(42)
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Inside Stain Kit
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Product Specs
Item
Inside Stain Kit
Company
Miltenyi Biotec
Price
$275.00
Get Pricing
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Catalog Number
130-090-477
Applications
Flow cytometry
Type
Kits and assays
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Local Office
Miltenyi Biotec
Miltenyi Biotec Inc.
2303 Lindbergh Street
Auburn
,
CA
95602
United States
Phone
: Phone: +1 530 888 8871
Toll Free: 800 FOR MACS
Fax: +1 530 888 8925
Email: macs@miltenyibiotec.com
Company Profile
Website:
www.miltenyibiotec.com
International Office
Miltenyi Biotec
Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429
Bergisch Gladbach
Germany
Phone
: +49 2204 8306-0
Fax: +49 2204 8306-235
Email: macs@miltenyibiotec.de
Company Profile
Website:
www.miltenyibiotec.com
Citations (42)
(1)
Therapeutic delivery of albumin-binding siRNA targeting IRS2 to diverse cell types reduces mammary tumor growth
Molecular Therapy
January 29, 2026
Claire E. Tocheny, Julianna E. Buchwald, Christopher D. Dahlke, Hassan H. Fakih, Jennifer S. Morgan, Ashley Summers, Christi A. Silva, Samuel O. Jackson, Ji-Sun Lee, Michael-Anthony Card,
et al.
Viability Dye (
Invitrogen
#
65–0865-14
) and fixed using a formaldehyde-based fixative (
Miltenyi
Cat#
130–090-477
). Samples were run on a Cytek Aurora flow cytometer and analyzed using FlowJo v10 software. Single-stained
(2)
Simultaneous secretion of a PD-L1 x 4-1BB bispecific antibody improves antileukemic efficacy of STAb-T cells secreting a CD19-specific T-cell engager
Oncoimmunology
December 31, 2025
Marina Gómez-Rosel, Laura Rubio-Pérez, Ivana Zagorac, Carmen Domínguez-Alonso, Laura Díez-Alonso, Oana Hangiu, Rocío Navarro, Raúl Torres-Ruíz, Sandra Rodríguez-Perales, Francisco J. Blanco,
et al.
APC-conjugated
anti-His
mAb. Intracellular bsAbs were detected using the
Inside Stain Kit
(Cat. No.
130−090−477
;
Miltenyi Biotec
), following the manufacturer’s instructions, and an
anti-His
APC mAb. Alternatively,
(3)
Loss of ADAM15 prevents necroptosis induction by partial RIPK1 degradation due to enhanced TNF-R1 surface expression and basal caspase-8 activation
Cell Commun Signal
December 4, 2025
S. Braun, K. Knackfuß, T. Ziesmann, L. Mlinzk, A. Goerg, J. Frankenheim, A. Walter, W. Schneider-Brachert, U. Distler, J. Fritsch
located both inside cells and on the cell surface [46], we utilized the inside stain kit and protocol (
130–090–477
,
Miltenyi
). 1 × 106 cells were sedimented, washed with PBS (10 min, 300 × g), and resuspended in 250
(4)
Circulating tumour cells are a prognostic indicator in advanced high-grade serous ovarian cancer and are associated with platelets and immune cells following dissemination
British Journal of Cancer
October 10, 2025
Mark P. Ward, Faye Lewis, Catherine O’Gorman, Lucy A. Norris, Sarah E. Lochrin, Laura E. Kane, Tanya E. Kelly, Bashir M. Mohamed, Ashitha Ramesh, Roisin O’Connor,
et al.
paraformaldehyde (Sigma Aldrich, Ireland) followed by
incubation
with permeabilization buffer Inside Perm (Cat:
130-090-477
,
Inside stain Kit
;
Miltenyi Biotec
, Germany). For metastatic
breast cancer
patient samples, slides were
(5)
Myocardial disarray drives metabolic inefficiency in human cardiomyocytes
Commun Biol
October 9, 2025
Charlène Jouve, Andrea Ruiz-Velasco, Erminia Donnarumma, Rémi Le Borgne, Iman Momken, Céline Pereira, Magali Seguret, Eva Vermersch, Elodie Vimont, Ivan Nemazanyy,
et al.
manufacturer’s protocol. Then, collected cell pellets were fixed and permeabilized using
Inside Stain kit
(
130-090-477
Miltenyi Biotech
) for 10 min at room temperature. Cells were incubated with fluorescent labeled antibodies
(6)
GMP-Compliant Process for the Manufacturing of an Extracellular Vesicles-Enriched Secretome Product Derived From Cardiovascular Progenitor Cells Suitable for a Phase I Clinical Trial
J Extracell Vesicles
August 20, 2025
Camille Humbert, Chloé Cordier, Iouri Drut, Michele Hamrick, Jacquelyn Wong, Valérie Bellamy, Justine Flaire, Kiranmayee Bakshy, Florent Dingli, Damarys Loew,
et al.
manufacturer's instructions. Intracellular
staining
was performed using
Inside stain kit
(
Miltenyi Biotec
, #
130‐090‐477
) according to the manufacturer's instructions.
Flow cytometric
analyses were performed on day+3 and
(7)
Frequency of synaptic antigen-specific CD4+ T cells in dementia is age-dependent but not correlated with cognitive impairment
Immun Ageing
June 19, 2025
Julius Hoffmann, Marie-Luise Machule, Jakob Kreye, Laura Stöffler, Péter Körtvelyessy, Maria Buthut, Rosa Rößling, Petra Bacher, Alexander Scheffold, Harald Prüss
Miltenyi
,
130–109–812
). Following cell permeabilization and fixation (Miltenyi inside stain kit,
Miltenyi
130–090–477
), intracellular epitopes were stained with antibodies targeting
CD154
(PE,
Miltenyi
, 5 C8,
130–113–607
,
(8)
Distinct HLA Haplotypes Are Associated With an Altered Strength of SARS-CoV-2-Specific T-Cell Responses and Unfavorable Disease Courses
Eur J Immunol
April 21, 2025
C. Dörnte, A. Datsi, V. Traska, J. Kostyra, M. Wagner, O. Brauns, C. Lamsfuß, H. Winkels, V. Balz, J. Enczmann,
et al.
markers, cell fixation was performed using Inside Fix (
Inside Stain Kit
,
Miltenyi Biotec
, cat. no.
130‐090‐477
), according to the manufacturer's instruction, followed by resuspension in 250 µL/well Inside Perm (
Inside Stain Kit
(9)
Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cells
Stem Cell Reports
October 8, 2024
Zeina R. Al Sayed, Charlène Jouve, Magali Seguret, Andrea Ruiz-Velasco, Céline Pereira, David-Alexandre Trégouët, Jean-Sébastien Hulot
BioLegend
). The collected cell pellets were then fixed and permeabilized with the
Inside Stain kit
(
130-090-477
,
Miltenyi Biotec
) for 10 min at room temperature. Cells were incubated with either Allophycocyanin (APC)
(10)
A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancer
Molecular Oncology
August 6, 2024
Volga M Saini, Ezgi Oner, Mark P. Ward, Sinead Hurley, Brian David Henderson, Faye Lewis, Stephen P. Finn, Gerard J. Fitzmaurice, John J. O'Leary, Sharon O'Toole,
et al.
antibody [V9, Alexa Fluor 546, sc‐6260]) were added to 399 μL of permeabilization buffer Inside Perm (#
130‐090‐477
,
Inside stain Kit
;
Miltenyi Biotec
, Bergisch Gladbach, Germany), along with 3.5 μL of 1 mg·mL−1 Hoechst
(11)
A versatile high-throughput assay based on 3D ring-shaped cardiac tissues generated from human induced pluripotent stem cell-derived cardiomyocytes
eLife
April 5, 2024
Magali Seguret, Patricia Davidson, Stijn Robben, Charlène Jouve, Celine Pereira, Quitterie Lelong, Lucille Deshayes, Cyril Cerveau, Maël Le Berre, Rita S Rodrigues Ribeiro,
et al.
Then, collected cell pellets were fixed and permeabilized using
Inside Stain kit
(
Miltenyi Biotech
,
130-090-477
) at room temperature for 10 min. Cells were incubated with either APC anti-cardiac troponin T (CTNT)
(12)
Nicotinamide-Expanded Allogeneic Natural Killer Cells with CD38 Deletion, Expressing an Enhanced CD38 Chimeric Antigen Receptor, Target Multiple Myeloma Cells
Int J Mol Sci
December 7, 2023
Avishay Edri, Nimrod Ben-Haim, Astar Hailu, Nurit Brycman, Orit Berhani-Zipori, Julia Rifman, Sherri Cohen, Dima Yackoubov, Michael Rosenberg, Ronit Simantov,
et al.
fixation and permeabilization using an
Inside Stain Kit
(
Miltenyi
, Bergisch Gladbach, Germany. cat#
130-090-477
) according to the manufacturer’s instructions. After permeabilization, intracellular
staining
was performed
(13)
Inhibition of microfold cells ameliorates early pathological phenotypes by modulating microglial functions in Alzheimer's disease mouse model
Journal of neuroinflammation
November 27, 2023
Kim N, Ju IG, Jeon SH, Lee Y, Jung MJ, Gee MS, Cho JS, Inn KS, Garrett-Sinha LA, Oh MS,
et al
.
anti-
CD68
Alexa488 (
1:50
,
BioLegend
,
137012
) were performed with
Inside Stain kit
(
Miltenyi Biotec
,
130-090-477
) according to the manufacturer’s instructions. After an
incubation
period of 1 h at 4 °C,
fluorescence
(14)
Oral Delivery of mRNA Vaccine by Plant-Derived Extracellular Vesicle Carriers
Cells
July 11, 2023
Margherita A. C. Pomatto, Chiara Gai, Federica Negro, Lucia Massari, Maria Chiara Deregibus, Francesco Giuseppe De Rosa, Giovanni Camussi
acquisition. For intracellular cytokine analysis, cells were previously permeabilized using
Inside Stain Kit
(
130-090-477
,
Miltenyi
, Bologna, Italy). For cell proliferation, splenocytes were stained with CellTrace™ CFSE cell
(15)
Streptococcus pneumoniae drives specific and lasting Natural Killer cell memory
PLoS pathogens
July 1, 2023
Camarasa TMN, Torné J, Chevalier C, Rasid O, Hamon MA.
IFNγ, cells were permeabilized and stained in buffer from commercial kit (
Inside Stain Kit
, ref no.
130-090-477
,
Miltenyi Biotec
) for 40 minutes at 4°C. After a final wash suspended in DPBS, sample acquisitions were
(16)
Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering
The CRISPR Journal
June 1, 2023
Rebecca C. Lamothe, Meghan D. Storlie, Diego A. Espinosa, Rachel Rudlaff, Patrick Browne, Jason Liu, Andres Rivas, Audra Devoto, Jennifer Oki, Ashcon Khoubyari,
et al.
Attune NxT Flow Cytometer
(
ThermoFisher
). For
staining
of intracellular markers, the
Inside Stain kit
(
Miltenyi
No.
130-090-477
) was used according to the manufacturer's instructions. The following monoclonal antibodies were purchased
(17)
Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer's disease
Nature communications
March 24, 2023
Choi BJ, Park MH, Park KH, Han WH, Yoon HJ, Jung HY, Hong JY, Chowdhury MR, Kim KY, Lee J,
et al
.
anti-IL17 PerCp Cy5.5 (
1:100
,
BD Bioscience
,
553142
) were performed with
Inside Stain Kit
(
Miltenyi Biotec
,
130-090-477
) according to the manufacturer’s instructions. Analysis was performed using
Attune NxT flow cytometer
(18)
Melatonin Activates Anti-Inflammatory Features in Microglia in a Multicellular Context: Evidence from Organotypic Brain Slices and HMC3 Cells
Biomolecules
February 16, 2023
Merlo S, Caruso GI, Korde DS, Khodorovska A, Humpel C, Sortino MA.
and
Cell Counts
on HMC3 Cells Cells were fixed with InsideFix (
Miltenyi
, Bologna, Italy; Cat. No.
130-090-477
) and incubated with the primary antibody rabbit anti-SIRT1 (
1:80
; Abcam, Cambridge, United Kingdom;
(19)
Chemotherapeutics Used for High-Risk Neuroblastoma Therapy Improve the Efficacy of Anti-GD2 Antibody Dinutuximab Beta in Preclinical Spheroid Models
Cancers (Basel)
January 31, 2023
Sascha Troschke-Meurer, Maxi Zumpe, Lena Meißner, Nikolai Siebert, Piotr Grabarczyk, Hannes Forkel, Claudia Maletzki, Sander Bekeschus, Holger N. Lode
Viobility™ 405/452 Fixable Dye
(
1:100
in 1× PBS, 15 min). Cells were fixed by using
Inside Stain Kit
(
Miltenyi Biotec
,
130-090-477
) according to manufacturer’s guidelines. A total of 10 µL of Tandem Signal Enhancer was added prior
(20)
Impaired activation of plasmacytoid dendritic cells via toll-like receptor 7/9 and STING is mediated by melanoma-derived immunosuppressive cytokines and metabolic drift
Frontiers in immunology
January 1, 2023
Monti M, Ferrari G, Grosso V, Missale F, Bugatti M, Cancila V, Zini S, Segala A, La Via L, Consoli F,
et al
.
antibodies. Then, pDCs were fixed and permeabilized using the
Inside Stain Kit
(
Miltenyi Biotec
; cat. No.
130-090-477
) and intracellular
staining
was performed using the anti-IFN-α (
Miltenyi Biotec
; cat. No.
130-116-874
)
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Inside Stain Kit from Miltenyi Biotec
Get Pricing
Get Quote for this Product
$275.00
Product Specs
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