Sodium Orthovanadate (Vanadate) from New England Biolabs

Product Specs
ItemSodium Orthovanadate (Vanadate)
CompanyNew England Biolabs
Price $36.00 Inquire
Catalog NumberP0758S
Targettyrosine phosphatase
Quantity1 ml
ApplicationsProteomics & Glycomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The COX2-PGE2-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING ActivationbioRxivApril 6, 2026Pham Thuy Tien Vo, Julien Cicero, Zichen Wang, Hiroyuki Hakozaki, Thomas S Hoang, Sendi Rafael Adame-Garcia, Dana J. Ramms, Kuniaki Sato, Erica Stevenson, Yuan Zhou, et al.protease and phosphatase inhibitor cocktail (ThermoScientific, 78446) and sodium orthovanadate (NEB, P0758S). Cell extracts were scraped, collected, and centrifuged at 16,000xg for 10 minutes at 4°C. Supernatants

(2) Vinorelbine enhances the efficacy of oncolytic vaccinia virus in a preclinical model of ovarian high-grade serous carcinomaMolecular Therapy OncologyMarch 19, 2026Stephanie Drymiotou, Christophe J. Queval, Katherine E. Tyson, Lesley A. Sheach, Antonio Postigo, Ilaria Dalla Rosa, Darren P. Ennis, Michael Howell, Iain A. McNeish, Michael Wayexamination of phosphorylated proteins, 1 mM orthovanadate (New England Biolabs, Ipswich, Massachusetts, #P0758) was added during blocking primary and secondary antibody incubation. The membranes were incubated with

(3) Genome-wide CRISPR Screening Reveals a PKA-Driven Resistance Mechanism to Metformin for Oral Cancer Prevention That Can Be Exploited by Combination with NSAIDsCancer Prevention ResearchFebruary 6, 2026Thomas S. Hoang, Farhoud Faraji, Amaya N. Mendez-Molina, Sendi Rafael Adame-Garcia, Kuniaki Sato, Tomohiko Ishikawa, Pham Thuy Tien Vo, Sydney I. Ramirez, Paola Y. Anguiano Anguiano Quiroz, Tracy Guo, et al.Cocktail (Thermo Fisher Scientific, cat. #78440) and 1 mM Sodium Orthovanadate (New England Biolabs, cat. #P0758S). Lysates were clarified by centrifugation at maximum speed at 4°C. Protein concentrations were determined

(4) Protein kinase a regulates cyclooxygenase-2 expression through the RNA-binding proteins HuR and TTPJournal of Biological ChemistryFebruary 1, 2026Sendi Rafael Adame-Garcia, Thomas S. Hoang, Pham Thuy Thien Vo, Valeria Burghi, Rodolfo Daniel Cervantes-Villagrana, Lennis Beatriz Orduña-Castillo, Dana J. Ramms, JoAnn Trejo, J. Silvio Gutkindand phosphatase inhibitor cocktail (Thermo Fisher Scientific, 78446) and sodium orthovanadate (NEB, P0758S). Cell extracts were scraped, collected, and centrifuged at 16,000g for 10 min at 4 °C. Supernatants

(5) Unraveling Resistance Mechanisms to Gαq Pathway Inhibition in Uveal Melanoma: Insights from Signaling-Activation Library ScreeningCancers (Basel)December 25, 2025Simone Lubrano, Rodolfo Daniel Cervantes-Villagrana, Nadia Arang, Elena Sofia Cardenas-Alcoser, Kuniaki Sato, Gabriela Cuesta-Margolles, Justine S. Paradis, Monica Acosta, J. Silvio Gutkind(ThermoFisher Scientific, Waltham, MA, USA, 78440) and 1mM sodium orthovanadate (New England Biolabs, Ipswich, MA, USA, P0758S). The lysates were centrifuged at maximum speed at 4 °C, and protein concentrations were determined

(6) HSP90 buffers deleterious genetic variations in BRCA1Molecular CellDecember 4, 2025Brant Gracia, Xing-Han Zhang, Patricia Montes, Tin Chanh Pham, Min Huang, Junjie Chen, Georgios Ioannis KarrasPepstatin A, VWR 97064–248; PMSF, Sigma‐Aldrich 7110), phosphatase inhibitors (Sodium Orthovanadate, NEB P0758S; Sodium Fluoride, Sigma‐Aldrich S7920), RNase A (Sigma‐Aldrich R6513), and Benzonase (MilliporeSigma

(7) Schlafen 12 Modulation and Targeting in Acute Myeloid LeukemiaCancer Research CommunicationsNovember 1, 2025Jamie N. Guillen Magaña, Markella Zannikou, Aneta Baran, Sara Small, Michael Schieber, Matthew J. Schipma, Elizabeth T. Bartom, Masha Kocherginsky, Diana Saleiro, Elspeth M. Beauchamp, et al.#sc-482875), 100 mmol/L of NaF (Sigma-Aldrich, cat. #S1504), 200 μmol/L Na3VO4 (New England Biolabs, cat. #P0758S), protease inhibitor cocktail set III (EMD Millipore, cat. #539134), and phosphatase inhibitor cocktail

(8) Modelling arrhythmogenic cardiomyopathy fattyfibro pathology with PKP2-deficient epicardial cells derived from human iPSCsCommun BiolOctober 27, 2025Sadia L. Falana, Sobhi G. Kazmouz, Jessika B. Iwanski, Sailu Sarvagalla, Blaire E. Bas, Elizabeth Juneman, Talal Moukabary, Ning Ma, Rebekah L. Gundry, Leili Rohani, et al.Triton X-100, and protease inhibitor (Cat no: 11836153001, Sigma Aldrich) and sodium orthovanadate (Cat no: P0758S, New England BioLabs)]. Protein concentration was determined using the Pierce BCA Protein Assay. Sodium

(9) Inhibition of anti-apoptotic BCL2 overcomes adaptive resistance to co-targeting of the protein kinase FAK and MEK in GNAQ-driven uveal melanomaJournal of Biological ChemistrySeptember 11, 2025Simone Lubrano, Rodolfo Daniel Cervantes-Villagrana, Nadia Arang, Adam Officer, Sendi Rafael Adame-Garcia, Gabriela Cuesta-Margolles, Andrew E. Aplin, J. Silvio GutkindInhibitor Cocktail (ThermoFisher Scientific, #78440) and 1 mM Sodium Orthovanadate (New England Biolabs, P0758S). The lysates were centrifuged at maximum speed at 4 °C, and protein concentrations were determined

(10) Myosin-dependent short actin filaments contribute to peripheral widening in developing stereociliaNat CommunJuly 1, 2025Xiayi Liao, Chun-Yu Tung, Jocelyn F. Krey, Ghazaleh Behnammanesh, Joseph A. Cirilo, Mert Colpan, Christopher M. Yengo, Peter G. Barr-Gillespie, Jonathan E. Bird, Benjamin J. Perrinalbumin) and 0.05% saponin with or without 4 mM sodium orthovanadate54 (New England Biolabs, Cat. # P0758S). After a 1-minute incubation, His-TMOD1 (714 nM) was added to the solution and incubated at room temperature

(11) An unconventional autophagic pathway that inhibits ATP secretion during apoptotic cell deathNat CommunApril 10, 2025Elena Terraza-Silvestre, Raquel Villamuera, Julia Bandera-Linero, Michal Letek, Daniel Oña-Sánchez, Cristina Ramón-Barros, Clara Moyano-Jimeno, Felipe X. Pimentel-Muiños7.5, 150 mM NaCl, 5 mM EDTA, PIC (1/100), PMSF (1 mM), orthovanadate (200 nM; New England Biolabs, P0758S) and β-glycerophosphate (25 mM; Sigma, G9422). After a 10 min centrifugation step (16200 × g, 4 °C),

(12) FAK inhibition combined with the RAF-MEK clamp avutometinib overcomes resistance to targeted and immune therapies in BRAF V600E melanomaCancer CellMarch 10, 2025Simone Lubrano, Rodolfo Daniel Cervantes-Villagrana, Farhoud Faraji, Sydney Ramirez, Kuniaki Sato, Sendi R. Adame-Garcia, Adam Officer, Nadia Arang, Damiano C. Rigiracciolo, Paola Y. Anguiano Quiroz, et al.and Phosphatase Inhibitor Cocktail (#78440, Thermo Fisher Scientific) and 1mM Sodium Orthovanadate (P0758S, New England Biolabs). Lysates were centrifuged at max speed at 4°C, concentrations were measured using

(13) FABP4 as a therapeutic host target controlling SARS-CoV-2 infectionEMBO molecular medicineMarch 1, 2025Baazim H, Koyuncu E, Tuncman G, Burak MF, Merkel L, Bahour N, Karabulut ES, Lee GY, Hanifehnezhad A, Karagoz ZF, et al.RIPA buffer (ThermoFisher Scientific, 89900), supplemented with orthovanadate (New England Biolabs, P0758L) and protease inhibitor cocktail (Sigma-Aldrich, P8340). Protein concentrations were determined using

(14) The Human ABCG2 Transporter Engages Three Gates to Control Multidrug ExtrusioniScienceFebruary 28, 2025Narakorn Khunweeraphong, Karl KuchlerMedical Products Vertriebs GmbH,Cat#MCE-HY-19753 Sodium Orthovanadate,New England Biolabs GmbH,Cat#P0758S 7-Dehydrocholesterol,Santa Cruz Biotechnology,Cat#sc-214398 cOmplete™ Proteasehemmer-Cocktail,Sigma-Aldrich,Cat#4693116001 "30% Acrylamide/Bis Solution, 37.5:1

(15) Methods for investigating STAT3 regulation of lysosomal function in mammary epithelial cellsJournal of Mammary Gland Biology and NeoplasiaDecember 1, 2024Bethan Lloyd-Lewis, Michael E. D’Angelo, Neve B. Prowting, Bethan E. Wiseman, Timothy J. Sargeant, Christine J. Watsonat 4 °C. Prior to use, add cOmplete Protease Inhibitor Cocktail, 1 mM sodium orthovanadate (NaVO3, P0758L, NEB) and 50 mM sodium fluoride (NaF, 67414-1ML-F, Merck).Magnetic microcentrifuge tube rack. Measuring

(16) Structure of the interleukin-5 receptor complex exemplifies the organizing principle of common beta cytokine signalingMolecular CellMay 16, 2024Nathanael A. Caveney, Grayson E. Rodriguez, Christoph Pollmann, Thomas Meyer, Marta T. Borowska, Steven C. Wilson, Nan Wang, Xinyu Xiang, Karsten D. Householder, Pingdong Tao, et al.were lysed on ice for 10 min with Pierce IP Lysis Buffer (#87787, Thermo) supplemented with Vanadate (P0758L, New England Biolabs), Halt Protease Inhibitor Cocktail (#78430, Thermo), Phosphatase Inhibitor Cocktail I

(17) miR-147b mediated suppression of DUSP8 promotes lung cancer progressionOncogeneApril 1, 2024Turkowski K, Herzberg F, Günther S, Weigert A, Haselbauer T, Fink L, Brunn D, Grimminger F, Seeger W, Sültmann H, et al.phenylmethylsulfonyl fluoride (Sigma- Aldrich, 93482-250ML-F, St. Louis, MO, USA), and sodium orthovanadate (NEB, P0758S, New England Biolabs, Frankfurt, Germany). Tumor tissue was disrupted with ceramic beads, and cell lysates

(18) Protein-folding chaperones predict structure-function relationships and cancer risk in BRCA1 mutation carriersCell ReportsFebruary 27, 2024Brant Gracia, Patricia Montes, Angelica Maria Gutierrez, Banu Arun, Georgios Ioannis Karras97064–248; PMSF, Sigma-Aldrich, 7110), phosphatase inhibitors (Sodium Orthovanadate, New England BioLabs, P0758; Sodium Fluoride, Sigma-Aldrich, S7920), RNAse A (Fisher Scientific, EN0531) and Benzonase (MilliporeSigma,

(19) Multisite phosphorylation dictates selective E2-E3 pairing as revealed by Ubc8/UBE2H-GID/CTLH assembliesMolecular CellJanuary 18, 2024Jakub Chrustowicz, Dawafuti Sherpa, Jerry Li, Christine R. Langlois, Eleftheria C. Papadopoulou, D. Tung Vu, Laura A. Hehl, Özge Karayel, Viola Beier, Susanne von Gronau, et al.lung,Sigma,Cat#A1153-10MG Leupeptin,Sigma,Cat#L2884-250MG Sodium Fluoride,Sigma,Cat#201154-100G β-glycerophosphate,Sigma,Cat#G9422-100G Orthovanadate,NEB,Cat#P0758S CK2,NEB,Cat#P6010L Lambda phosphatase,NEB,Cat#P0753L Silmitasertib (CX-4945),Selleckchem,Cat#S0707 SML0795,Sigma,Cat#SML0795-5MG PZ0313,Sigma,Cat#PZ0313-5MG Phos-tag

(20) Understanding the Immunomodulatory Effects of Bovine Colostrum: Insights into IL-6/IL-10 Axis-Mediated Inflammatory ControlVeterinary sciencesAugust 11, 2023Grigalevičiūtė R, Matusevičius P, Plančiūnienė R, Stankevičius R, Radzevičiūtė-Valčiukė E, Balevičiūtė A, Želvys A, Zinkevičienė A, Zigmantaitė V, Kučinskas A, et al.#10010023, ThermoFisher Scientific, Waltham, MA, USA) containing 1 mM sodium orthovanadate (NEB, # P0758S, New Engalnd Biolabs, Ipswich, England). The membrane from the transwell inserts was cut using forceps

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