Rabbit Reticulocyte Lysate System, Nuclease Treated from Promega

Product Specs
ItemRabbit Reticulocyte Lysate System, Nuclease Treated
CompanyPromega
Price $134.00Supplier Page View Company Product Page
Catalog NumberL4960
Quantity30 reactions
SystemRabbit reticulocyte lysate
Molecular Weight~88kDa
SpeciesRabbit

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulationNucleic Acids ResApril 21, 2026Caroline A McCormick, Michele Meseonznik, Yuchen Qiu, Oleksandra Fanari, Priyanka Goyal, Mitchell Thomas, Mina Shokoufandeh, Yifang Liu, Dylan Bloch, Cole Greenfield, et al.hypermodified RNAs was performed using the nuclease-treated rabbit reticulocyte lysate (RRL) system (Promega, L4960). Both positive (Fluc mRNA from the RRL kit) and negative (no mRNA) controls were run simultaneously.

(2) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin LeppekcircRNA was used throughout all in vitro translation systems. For the Promega WGL (L4380) and RRL (L4960) extracts, 10–500ng of linear Fluc mRNA and Nluc-encoding circRNA were used at a ratio of 1:10. Using

(3) RNA-specific local translation is patterned by condensates for multinucleate cell growthNat Cell BiolMarch 2, 2026Zachary M. Geisterfer, Ameya P. Jalihal, Sierra J. Cole, Amy S. Gladfelterincubate for 10 min at room temperature before supplementing the reaction 1:1 with RR extract (Promega, L4960). The reactions were incubated at 30 °C for 1 h before the addition of substrate, Vivazine (Promega,

(4) A bicistronic viral genome uses a compact type IV IRES near its 3′ end to express a transmembrane proteinCell ReportsFebruary 24, 2026Madeline E. Sherlock, Katherine E. Segar, Jeffrey S. Kieftcommercial assays,Critical commercial assays "Rabbit Reticulocyte Lysate, Nuclease-Treated",Promega,L4960 Dual-Glo Luciferase Assay System,Promega,E2920 Deposited data,Deposited data,Deposited data CryoEM

(5) Structural basis for non-AUG translation regulation by 5MPsbioRxivDecember 31, 2025Ximena Zottig, Chun-Ying Huang, Zahra Seraj, Nikolaus Grigorieff, Andrei A. KorostelevRRL In vitro translation was performed using a commercial micrococcal-nuclease-treated RRL (Promega; L4960) with modifications as described below. Translation reactions were carried out in the presence of 50%

(6) Antisense oligonucleotide-based switches for conditional translation of synthetic mRNAMolecular Therapy Nucleic AcidsDecember 9, 2025Francis Combes, Frida J Pettersson, Thanh-Huong BuimRNA was evaluated on agarose gel. RRL assays Nuclease-treated RRL kits were purchased at Promega (#L4960). DNA leashes were annealed to PMOs at 1:1 molar ratios using a thermocycler as described in ref. 24.24

(7) Understanding off-target growth defects introduced to influenza A virus by synonymous recodingRNANovember 1, 2025Colin P. Sharp, Beth H. Thompson, Ananya Ferdous Hoque, Ola Diebold, Blanka Tesla, Dominic Kurian, Peter Simmonds, Paul Digard, Eleanor Gauntassays were performed using Rabbit Reticulocyte Lysate kit with one-fifth volume reactions (Promega, #L4960). For western blotting, antibody α-tubulin (clone YL1/2, Bio-Rad, #MCA77G) was used. PCR amplicon purification

(8) A family of lethal exotoxins defined by cell entry via the Attractin receptorbioRxivOctober 14, 2025Raghuvir Viswanatha, Donghoon Lee, William R. Robins, Enzo Mameli, Yanhui Hu, Ah-Ram Kim, Yousuf Hashmi, Hiroshi Nishida, Gyan Prakash, Matthew Butnaru, et al.protein, MBP) or purified toxins were added to nuclease-treated Rabbit Reticulocyte Lysate (Promega, L4960) programmed with firefly luciferase mRNA (ApexBio). Following incubation for the indicated times, luciferase

(9) DDX6 interacts with DDX3X to repress translation in microRNA-mediated silencingNucleic Acids ResearchSeptember 9, 2025Yanyan Lu, Meng Tao, Hong Su, Yiren Tu, Ji-Ping Wang, Masahiko Kuroda, Xiaozhong Wangtranslation In vitro translation was performed using the Rabbit Reticulocyte Lysate system (Promega, L4960) according to the manufacturer’s protocol. Purified Ff-Luc and Rn-Luc mRNAs were first denatured at

(10) NAT10 Increases Lysosomal Acidification to Promote Esophageal Cancer Metastasis via ac4C Acetylation of ATP6V0E1 mRNAAdvanced ScienceJuly 29, 2025Yu‐Juan Zhan, Chun‐Miao Deng, Lin Tang, Shu‐Jun Li, Tao‐Yang Xu, Xian Wei, Xin‐Yi Zhang, Can‐Can Zheng, Li Deng, Cui Shao, et al.incubating 2 µg of either acetylated or unmodified mRNA templates with 35 µL of rabbit reticulocyte lysate (L4960, Promega) in a final volume of 50 µL. The efficiency of translation was determined by Western blot analysis. Luciferase Reporter Assay

(11) Efficient cell-free translation from diverse human cell typesJournal of Biological ChemistryJuly 1, 2025Jana Ziegelmüller, Nikolaos Kouvelas, Nino Schwaller, Priyanka Thambythurai, Alexander M. Hofer, Oliver Mühlemann, Evangelos D. Karousisin vitro translation reactions were carried out according to the manufacturer’s guidelines (Promega, Cat.No. L4960) for 60 min at 30 °C. For the comparison with RRL, the HEK FITR and HeLa S3 in vitro translation reactions

(12) Chemical and topological design of multicapped mRNA and capped circular RNA to augment translationNature BiotechnologyJuly 1, 2025Hongyu Chen, Dangliang Liu, Abhishek Aditham, Jianting Guo, Jiahao Huang, Franklin Kostas, Kamal Maher, Mirco J. Friedrich, Ramnik J. Xavier, Feng Zhang, et al.protocol mentioned above. In vitro translation was conducted using RRL (nuclease-treated; Promega, L4960). Then, 40% RRL, 4% SUPERaseIn RNase inhibitor, amino acid mixture and indicated mRNA were added to

(13) Inhibitors of eIF1A-ribosome interaction unveil uORF-dependent regulation of translation initiation and antitumor and antiviral effectsThe EMBO JournalMay 12, 2025Daniel Hayat, Ariel Ogran, Shaked Ashkenazi, Alexander Plotnikov, Roni Oren, Mirie Zerbib, Amir Ben-Shmuel, Rivka DiksteinRiboMaxTM,Promega,#P1280 Vaccinia capping kit,New England Biolabs,#M2080S Rabbit Reticulocytes lysate,Promega,#L4960 PLA kit - far red,Sigma-Aldrich,#Duo92101 Cell TiterGlo viability assay,Promega,#G7570 Reagents

(14) A structural element within the 5′UTR of β-catenin mRNA modulates its translation under hypoxiaNucleic Acids ResMay 1, 2025Mattia D’Agostino, Javier Rol-Moreno, Guillaume Bec, Lauriane Kuhn, Eric Ennifar, Angelita SimonettiLysate (RRL) [44, 45] In vitro translation assays In-house prepared HCTSs and nuclease-treated RRL (L4960, Promega) were utilized for in vitro translation. Reaction mixture contained 12.5 mg/ml of cell extract

(15) A toolbox for ablating excitatory and inhibitory synapsesElifeApril 29, 2025Aida Bareghamyan, Changfeng Deng, Sarah Daoudi, Shubhash C Yadav, Xiaocen Lu, Wei Zhang, Robert E Campbell, Richard H Kramer, David M Chenoweth, Don B Arnold(Schleicher & Schuell BioScience). The library was translated with rabbit reticulocyte lysate (Promega cat. # L4960) and purified with dT-25 BIOTEG beads (NEB cat. # S1419), eluted in water, and desalted with Centrisep

(16) A broad-spectrum lasso peptide antibiotic targeting the bacterial ribosomeNatureApril 24, 2025Manoj Jangra, Dmitrii Y. Travin, Elena V. Aleksandrova, Manpreet Kaur, Lena Darwish, Kalinka Koteva, Dorota Klepacki, Wenliang Wang, Maya Tiffany, Akosiererem Sokaribo, et al.lysate The effect of LAR on eukaryotic translation was assessed using Rabbit Reticulocyte Lysate System (L4960, Promega) programmed with firefly luciferase mRNA. In vitro reactions were assembled according to the

(17) A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformationMolecular CellMarch 6, 2025Raja H. Ali, Esteban A. Orellana, Su Hyun Lee, Yun-Cheol Chae, Yantao Chen, Jim Clauwaert, Alyssa L. Kennedy, Ashley E. Gutierrez, David J. Papke, Mateo Valenzuela, et al.reticulocyte assay for in vitro translation Thirty-five μl of nuclease treated rabbit reticulocytes lysate (L4960, Promega) were incubated for 30 minutes at 30°C together with 1 μM amino acid mix (L4960, Promega) 40

(18) A conserved class of viral RNA structures regulates translation reinitiation through dynamic ribosome interactionsCell ReportsFebruary 25, 2025Madeline E. Sherlock, Conner J. Langeberg, Katherine E. Segar, Jeffrey S. Kieftcommercial assays,Critical commercial assays "Rabbit Reticulocyte Lysate, Nuclease-Treated",Promega,L4960 2X PerfeCTa SYBR Green FastMix,Quantabio,95072 Deposited data,Deposited data,Deposited data CryoEM

(19) A toolbox for ablating excitatory and inhibitory synapsesbioRxivJanuary 31, 2025Aida Bareghamyan, Changfeng Deng, Sarah Daoudi, Shubash C. Yadav, Xiaocen Lu, Wei Zhang, Robert E. Campbell, Richard H. Kramer, David M. Chenoweth, Don B. Arnold(Schleicher & Schuell BioScience). The library was translated with rabbit reticulocyte lysate (Promega cat. # L4960) and purified with dT-25 BIOTEG beads (NEB cat. # S1419), eluted in water, and desalted with Centrisep

(20) Endocytic recycling is central to circadian collagen fibrillogenesis and disrupted in fibrosiseLifeJanuary 15, 2025Chang J, Pickard A, Herrera JA, O'Keefe S, Garva R, Hartshorn M, Hoyle A, Dingle L, Knox J, Jowitt TA, et al.Biosystems,RE7270-RE, Commercial assay or kit,"Rabbit reticulocyte lysate, nuclease treated",Promega,L4960, Commercial assay or kit,TaqMan Reverse Transcription kit,Applied Biosystems,N8080234, Commercial

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