Riboprobe Combination System-SP6/T7 RNA Polymerase from Promega

Product Specs
ItemRiboprobe Combination System-SP6/T7 RNA Polymerase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP1460
Quantity1 system

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Proteomic analysis of the zone of degeneration at the mitosis–meiosis transition stage in wild‐caught male catshark ( Scyliorhinus canicula ), correlated with an unusually high‐water temperature in the English ChannelJournal of Fish BiologyNovember 12, 2025Fabian Jeanne, Stanislas Pilet, Sean Heighton, Benoît Bernay, Pascal SourdaineScfshr‐cDNA or Sclhr‐cDNA cloned in pCR™II‐TOPO™ using the Riboprobe® Combination Systems (Promega, reference P1460), and DNAs were then digested with RQ1 DNAse (Promega, reference M6101). Paraffin sections (7 μm) were

(2) Human adipose stromal cells differentiate towards a tendon phenotype with adapted visco-elastic properties in a 3D-culture systemBiology OpenMay 15, 2025Maxime Hordé, Jonathan Fouchard, Luna Gomez Gomez Palacios, Xavier Laffray, Cédrine Blavet, Véronique Béréziat, Claire Lagathu, Ludovic Gaut, Delphine Duprez, Emmanuelle Havis2005). COL1A1, SCX, THBS2 and COL3A1 and probes were generated using a Roboprobe kit (Promega; ref P1460), according to manufacturer's instructions. Images were captured using a Nanozoomer SQ Digital Slide

(3) DYRK1A Up-Regulation Specifically Impairs a Presynaptic Form of Long-Term PotentiationLife (Basel)January 22, 2025Aude-Marie Lepagnol-Bestel, Simon Haziza, Julia Viard, Paul A. Salin, Arnaud Duchon, Yann Herault, Michel Simonneauused to generate antisense or sense [α35S]-rUTP (800 Ci/mmol, Amersham)-labeled transcripts, using the P1460 riboprobe in vitro transcription systems (Promega), according to the manufacturer’s instructions. Paraffin-embedded

(4) Emergence and increased epidemic potential of dengue variants with the NS5V357E mutation after consecutive years of transmissioniScienceNovember 15, 2024Hui-Ying Ko, Yao-Tsun Li, Han-Peng Yu, Ya-Yuan Li, Ming-Tsai Chiang, Yogy Simanjuntak, Yi-Ling Lee, Shih-Syong Dai, Pei-Jung Chung, Guann-Yi Yu, et al.Green Master Mix,Applied Biosystems,Cat#4344463 Riboprobe In Vitro Transcription Systems kit,Promega,P1460 RNeasy mini kit,Qiagen,74104 KOD Hot Start DNA Polymerase kit,Merck/Novagen,71086 Streptavidin-Sepharose

(5) Limb connective tissue is organized in a continuum of promiscuous fibroblast identities during developmentiScienceJuly 19, 2024Estelle Hirsinger, Cédrine Blavet, Marie-Ange Bonnin, Léa Bellenger, Tarek Gharsalli, Delphine Duprezwere prepared. The SCX, OSR1, KLF2 and SPRY2 probes were synthetized using the Riboprobe kit (Promega P1460) and the linearization/polymerization enzymes EcoR1/T3, NotI/T3, EcoR1/T3 and EcoR1/T7, respectively,

(6) hoxa1a-Null Zebrafish as a Model for Studying HOXA1-Associated Heart Malformation in Bosley–Salih–Alorainy SyndromeBiology (Basel)June 23, 2023Hongjie Wang, Jingwei He, Xuemei Han, Xiuzhi Wu, Xuebin Ye, Wenchao Lv, Yao Zuamplified using cDNA from wild-type zebrafish as a template, and then purified for in vitro transcription (p1460, Promega, Madison, WI, USA) to synthesize the probes [24]. Hybridization: Fix zebrafish embryos. For

(7) MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome CompositionCell reportsNovember 5, 2019Busch JD, Cipullo M, Atanassov I, Bratic A, Silva Ramos E, Schöndorf T, Li X, Pearce SF, Milenkovic D, Rorbach J, et al.Prime-It II Random Primer Labeling Kit,Agilent,Cat#300385 Qubit Protein Assay Kit,Thermo Fisher,Cat#Q33211 Riboprobe® System - SP6/T7,Promega,Cat#P1460 ,, Experimental Models: Cell Lines,Experimental Models: Cell Lines,Experimental Models: Cell Lines ,, HeLa

(8) A long noncoding RNA cluster-based genomic locus maintains proper development and visual functionNucleic acids researchJuly 9, 2019Wang F, Ren D, Liang X, Ke S, Zhang B, Hu B, Song X, Wang X.(Ambion, Cat. No. AMB13345) and the sgRNAs synthesized with T7 Riboprobe Systems (Promega, Cat. No. P1460) were co-microinjected into one-cell zebrafish embryos. Genomic DNA was extracted from 10 embryos at

(9) Effects of androgen and oestrogen on IGF pathways controlling phallus growthReproductionJanuary 1, 2019Yu Chen, Hongshi Yu, Andrew J Pask, Asao Fujiyama, Yutaka Suzuki, Sumio Sugano, Geoff Shaw, Marilyn B Renfreelabelling Mix (Roche, Cat#11277073910) and generated with T7/ SP6 polymerase synthase kit (Promega, Cat #P1460). The sections were pre-hybridized for 2 h at 42°C and hybridized for 16–18 h at 42°C. Then, the sections

(10) Androgen and Oestrogen Affect the Expression of Long Non-Coding RNAs During Phallus Development in a MarsupialNon-Coding RNADecember 30, 2018Yu Chen, Yoko Kuroki, Geoff Shaw, Andrew J. Pask, Hongshi Yu, Atsushi Toyoda, Asao Fujiyama, Marilyn B. Renfreelabelling Mix (Roche, Cat#11277073910) and generated with T7/SP6 polymerase synthase kit (Promega, Cat #P1460). The sections were pre-hybridized for 2 h at 42 °C and hybridized for 16–18 h at 42 °C, sections washed

(11) MALAT1-miR663a negative feedback loop in colon cancer cell functions through direct miRNA-lncRNA bindingCell death & diseaseAugust 28, 2018Tian W, Du Y, Ma Y, Gu L, Zhou J, Deng D.pGEM-T vector, and transcribed by T7 RNA Polymerase using Riboprobe in vitro Transcription Systems (P1460, Promega, Madison, WI, USA). The RNA was labeled with PierceTM RNA 3′ End Desthiobiotinylation Kit (20163,

(12) hnRNPA2/B1 activates cyclooxygenase-2 and promotes tumor growth in human lung cancersMolecular OncologyNovember 19, 2015Yang Xuan, Jingshu Wang, Liying Ban, Jian-Jun Lu, Canhui Yi, Zhenglin Li, Wendan Yu, Mei Li, Tingting Xu, Wenjing Yang, Zhipeng Tang, Ranran Tang, Xiangsheng Xiao, Songshu Meng, Yiming Chen, Quentin Liu, Wenlin Huang, Wei Guo, Xiaonan Cui, Wuguo Deng

(13) Histone deacetylase activity is required for embryonic posterior lateral line developmentCell proliferationFebruary 1, 2014He Y, Wu J, Mei H, Yu H, Sun S, Shou J, Li H.and 5′-AATATCCCCCTCCGTTTCAC-3′. The probe was transcribed in vitro using SP6 RNA polymerase (cat. no. P1460; Promega) and prepared with a digoxigenin labelling kit. Whole-mount RNA in situ hybridization was performed

(14) SLC25A12 expression is associated with neurite outgrowth and is upregulated in the prefrontal cortex of autistic subjectsMolecular PsychiatryApril 1, 2008A-M Lepagnol-Bestel et al.(probe of 1171 bp linearized with BamHI) and PRKCB1 (probe of 608 bp linearized with SacII), using the P1460 riboprobe in vitro transcription system (Promega, Madison, WI, USA). Hybridization was carried out with

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Riboprobe® in vitro Transcription Systems From Promega
Our lab performs in situ hybridization on a regular basis using RNA probes. We work with frozen ... Read More