PAP PEN MaxTag™ Histo for IHC from Rockland Immunochemicals, Inc.

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Intended Use.

To provide a hydrophobic barrier when a circle is drawn around a specimen on the glass slide. Properties: 1. Heat resistance up to 120°C. 2. Ready to be used directly on the wet slides. 3. Applicable to microwave staining and in situ hybridization. 4. Circle made by PAP PEN is insoluble in alcohol, acetone. 5. Withstand rehydration and dehydration steps in alcohol. 6. If desired, circle can be removed by xylene after the staining procedure is completed.
Method for Paraffin Sections

Sections should be quickly deparaffinized by xylene, then washed out with ethanol. Dry or wipe away excess liquid around the section on the glass slide with tissue paper. Encircle the section on a glass slide with the PAP PEN, as shown in the illustration (right), and dry the circle for 1-2 minutes at room temperature. Then, soak the section in a phosphoric acid buffer solution (PBS or TBS) for about 5 minutes. Method for Frozen Sections: Glass slides must be cleaned with xylene and ethanol, or coated with egg-albumin, glycerine, or poly-L-lysine, before the PAP PEN is applied.
PAP PEN MaxTag™ Histo for IHC

To provide a hydrophobic barrier when a circle is drawn around a specimen on the glass slide. Properties: 1. Heat resistance up to 120°C. 2. Ready to be used directly on the wet slides. 3. Applicable to microwave staining and in situ hybridization. 4. Circle made by PAP PEN is insoluble in alcohol, acetone. 5. Withstand rehydration and dehydration steps in alcohol. 6. If desired, circle can be removed by xylene after the staining procedure is completed.

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PAP PEN MaxTag™ Histo for IHC

Description

PAP PEN is intended to provide a hydrophobic barrier when a circle is drawn around a specimen on the glass slide. Before use, hold the pen point up and press it with fingertip to take blocked air out from the pen barrel to prevent overflow. To start, press the tip of the pen straight down until fluid starts soaking the tip and then draw the circle around the specimen. Be sure to close the cap after use. Perform the dyeing work after the ink has dried well. If too much ink comes out, wipe it off

Intended Use.

Method for Paraffin Sections