CytoStim™, human from Miltenyi Biotec

(1) Human gut M cells resemble dendritic cells and present gluten antigenNatureDecember 10, 2025Daisong Wang, Sangho Lim, Willine J. van de Wetering, Carmen Lopez-Iglesias, Yuu Okura, Yuri Teranishi-Ikawa, Akihiko Mizoroki, Willem Kasper Spoelstra, Talya Dayton, Gijs J. F. van Son, et al.3 h later with 100,000 naive CD4+ T cells from unrelated healthy donors. CytoStim (Miltenyi Biotec, 130-092-173) was added following the manufacturer’s protocol. After 24 h, CD69 upregulation was assessed as described

(2) Trifunctional antibody-cytokine fusion protein formats for tumor-targeted combination of IL-15 with IL-7 or IL-21Frontiers in ImmunologyApril 30, 2025Annika M. Möller, Sarah Vettermann, Felix Baumann, Max Pütter, Dafne MüllerPEVio770, 130-110-680; anti-CD8 VioBlue, 130-110-683; anti-Granzyme B FITC, 130-101-355; CytoStim™ (130–092–173). Human IFN-γ (DY285) DuoSet® ELISA kit was purchased from R&D Systems. B16-FAP cells (transfectants

(3) Impact of a varied set of stimuli on a suite of immunological parameters within peripheral blood mononuclear cells: toward a non-animal approach for assessing immune modulation by materials intended for human useFrontiers in ToxicologyApril 26, 2024Stella Cochrane, Ramya Rajagopal, David Sheffield, Fay Stewart, Lindsay Hathaway, Nicholas M. Barnes, Omar Qureshi, John Gordon(Dermatophagoides pteronyssinus extract; Citeq Biologics, product code 02.01.85), and CytoStim (Miltenyi Biotec, 130-092-173) were stored at 4°C.• For B-cell assays: CpG ODN (InvivoGen, tlrl-2006), IL-15 (ImmunoTools, 11340157),

(4) Use of serum-free media for peripheral blood mononuclear cell culture and the impact on T and B cell readoutsFront ToxicolJanuary 1, 2024Cochrane S, Saib O, Sheffield D.Mitogen (PWM) at 5.0 μg per mL (Sigma-Aldrich L8777)• Cytostim (CS) at 1.2 μL per mL (Miltenyi Biotec 130–092-173) Except for PWM, which was reconstituted in PBS, the stimulation reagents were reconstituted in HyClone

(5) Early Omicron infection is associated with increased reinfection risk in older adults in long-term care and retirement facilitieseClinicalMedicineSeptember 1, 2023Jessica A. Breznik, Ahmad Rahim, Ali Zhang, Jann Ang, Hannah D. Stacey, Hina Bhakta, Rumi Clare, Li-Min Liu, Allison Kennedy, Megan Hagerman, et al.a negative media control (unstimulated) and a positive polyclonal stimulation control (CytoStim™; #130-092-173; Miltenyi Biotec). Samples were stained with fluorophore-conjugated monoclonal antibodies, captured

(6) A Straightforward Cytometry-Based Protocol for the Comprehensive Analysis of the Inflammatory Valve Infiltrate in Aortic StenosisInternational Journal of Molecular SciencesJanuary 22, 2023Pablo Álvarez-Heredia, José Joaquín Domínguez-del-Castillo, Irene Reina-Alfonso, Carmen Gutiérrez-González, Fakhri Hassouneh, Alexander Batista-Duharte, Antonio Trujillo-Aguilera, Rosalía López-Romero, Ignacio Muñoz, Rafael Solana, et al.(BD Becton Dickinson, cat. 555029. Franklin Lakes, USA, New Jersey);Cytostim human (Miltenyi, cat. 130-092-173. Bergisch Gladbach, Germany);EDTA (PanReac AppliChem, cat. A4892. Chicago, USA, Illinois);Glutamine

(7) Kinetics of immune responses elicited after three mRNA COVID-19 vaccine doses in predominantly antibody-deficient individualsiScienceNovember 18, 2022Ainsua-Enrich E, Pedreño-Lopez N, Bracke C, Ávila-Nieto C, Rodríguez de la Concepción ML, Pradenas E, Trinité B, Marfil S, Miranda C, González S, et al.D9885-100G BriteLite Plus Luciferase,PerkinElmer,Cat# 6066769 "CytoStim™, human",Miltenyi Biotec,Cat# 130-092-173 "PepTivator ® SARS-CoV-2 Prot_S1, research grade, RG 60nmol",Miltenyi Biotec,Cat# 130-127-048 Human TruStain FcX

(8) Naive and memory CD4(+) T cell subsets can contribute to the generation of human Tfh cellsiScienceJanuary 21, 2022Jeger-Madiot R, Vaineau R, Heredia M, Tchitchek N, Bertrand L, Pereira M, Konza O, Gouritin B, Hoareau-Coudert B, Corneau A, et al.130-095-363 "Recombinant IL-12, premium grade",Miltenyi,Cat# 130-096-798 "CytoStim™, human",Miltenyi,Cat# 130-092-173 Cell-IDTM Intercalator-Rh,Fluidigm,Cat# 201103B Cell-IDTM Cisplatin-198Pt,Fluidigm,Cat# 201198 Cell-IDTM

(9) Effects of increased wholegrain consumption on immune and inflammatory markers in healthy low habitual wholegrain consumersEuropean Journal of NutritionFebruary 1, 2016Antonios Ampatzoglou, Charlotte L. Williams, Kiranjit K. Atwal, Catherine M. Maidens, Alastair B. Ross, Frank Thielecke, Satya S. Jonnalagadda, Orla B. Kennedy, Parveen Yaqoobintervention Comparison Baseline End ΔRG Comparison ΔWG Comparison Mean (SEM) Mean (SEM) P-value Mean (SEM) Mean (SEM) P-value Mean (SEM) Mean (SEM) P-value Unstimulated 1.6 (0.2) 1.3 (0.2) 0.932 1.9 (0.4) 1.3 (0.1) 0.255 -0.2 (0.3) -0.6 (0.4) 0.293 ConA 6.9 (0.9) 8.0 (1.1) 0.654 8.6 (1.3) 7.0 (0.9) 0.72 1.3 (1.0) -1.6 (1.2) 0.079 CytoStim 54.1 (2.6) 56.3 (2.5) 0.783 53.9 (2.6) 53.4 (2.2) 1.000 1.8 (1.2) -0.4 (1.6) 0.535 Unstimulated 2.4 (0.2) 2.4 (0.2) 1.000 2.5 (0.2) 2.6 (0.3) 0.99 0.1 (0.2) 0.1 (0.3) 0.787 ConA 11.3 (1.3) 12.4 (1.1) 0.883 11.8 (1.5) 11.5 (1.2) 0.999 1.3 (0.8) -0.3 (0.8) 0.174 CytoStim 55.1 (2.2) 56.1 (2.2) 0.992 54.2 (2.1) 53.9 (1.9) 1.000 0.5 (1.1) -0.3 (1.5) 0.855 CD4+ CD8+ WG, whole-grain; RG, refined grain; ConA, Concanavalin A; CytoStim, Miltenyi Biotec Ltd.-130-092-173 Within the RG and WG interventions, comparisons were performed between baseline and end measurements,

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CytoStim™, human from Miltenyi Biotec

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CytoStim™, human from Miltenyi Biotec

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