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Cloning and Expression
Library Construction
Genomic DNA
Genomic DNA
Human Genomic DNA
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Human Genomic DNA
Human Genomic DNA
Human Genomic DNA from Promega
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Citations:
(89)
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Promega for
Human Genomic DNA
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Description
Purified human genomic DNA. Greater than 90% of the DNA is longer than 50kb in size
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Human Genomic DNA
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G3041
Quantity
100microg
Molecular Weight
~125kDa
DNA Type
Genomic DNA
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (89)
(1)
Long-read target enrichment sequencing for rAAV integration site analysis in engineered clones with targeted viral insertion
Mol Ther Nucleic Acids
September 8, 2026
Erica Coratella, Myriam Lemmens, Mevion Oertli, Yoann Gilbart, Kristian Davidsen, Lara Bontadelli, Rebecca Bohnert, Joyce Rigal, Jennifer Bian, Tara Alpert,
et al.
estimation of short-read TES Genomic DNA from PC14 was serially diluted in commercial genomic DNA (#
G3041
,
Promega
, Madison, WI, USA) to final proportions ranging from 0.01% to 100% (corresponding to undiluted
(2)
Defects in intron recycling suppress the antiviral response via a mechanism of intronic endogenous dsRNA
Journal of Experimental Medicine
April 6, 2026
Chaorui Duan, Luke Buerer, Cory Bowers, Allison J. Taggart, Mara H. O’Brien, Sarah Gunasekera, Chien-Ling Lin, Jing Wang, Yi Zeng, Jonathan P. Staley,
et al.
in the same manner by
PCR amplification
of their respective templates from
human genomic DNA
(Cat#
G3041
;
Promega
), followed by IVT and annealing of the positive and negative strand RNAs. RNA
dot blot
The
(3)
Real-time PCR to target Hoodia in herbal supplements: a tool for conservation and trade regulation
Food Chemistry: Molecular Sciences
January 30, 2026
Marie-Alice Fraiture, Andrea Gobbo, Patrick Philipp, Nina Papazova, Céline Vanhee, Nancy H.C. Roosens
non-plant DNA samples belonging to animal and microbial species were also tested, including
Homo sapiens
(
G3041
,
Promega
, USA),
Bacillus licheniformis
(LMG 7558, BCCM, Belgium),
Aspergillus niger
(IHEM 2312, BCCM,
(4)
Comprehensive self-antigen screening to assess cross-reactivity in promiscuous T-cell receptors
Frontiers in Immunology
January 5, 2026
Govinda Sharma, Fei Teng, James Round, Sophie A. Sneddon, Sarania Sivasothy, Scott D. Brown, Robert A. Holt
library
cloning
procedures
. For
WES
library construction
, 1 µg of
human genomic DNA
(
Promega
cat. #
G3041
, lot #0000387521) was shipped to Genewiz for Illumina
whole exome-sequencing
library preparation only.
(5)
Extracellular mitochondrial DNA activates complement and is associated with complement activation in patients with out-of-hospital cardiac arrest
Scientific Reports
November 20, 2025
Eline de Boer, Anne-Lise Strandmoe, Marina Sokolova, Trond M. Michelsen, Huy Q. Quach, Viktoriia Chaban, Espen R. Nakstad, Geir Ø. Andersen, May-Kristin S. Torp, Kåre-Olav Stensløkken,
et al.
plasmid (15 min at 95 °C)17. To calculate nDNA copy numbers,
human genomic DNA
encoding for GAPDH (
G3041
,
Promega
, Madison, WI) was obtained. The standard curves were established by 10-fold serial dilutions
(6)
Quantifying the impact of genetic mutations on enhancer dynamics
bioRxiv
October 24, 2025
Maitreya Das, Deepro Banerjee, Ayaan Hossain, Matthew Jensen, Saie Mogre, Jiawan Sun, Joseph Mao, Adam B. Glick, Howard M. Salis, Santhosh Girirajan
major steps is provided below. (A) Shearing and fragment size selection
Human genomic DNA
(catalog #
G3041
,
Promega
, WI, USA) isolated from multiple anonymous male and female donors was sheared to an average
(7)
Recombinase polymerase amplification for single nucleotide polymorphism-specific detection of βC variant in sickle cell disease
Analytical Biochemistry
October 1, 2025
Mehnaz Urbee Jahangir, Megan M. Chang, Alexis Wilkinson, Zoha Wazir, Venée N. Tubman, Gladstone E. Airewele, Rebecca Richards-Kortum
design and optimization. For genotype AA, mixed-gender
human genomic DNA
was purchased from
Promega
(
G3041
).
Human genomic DNA
for genotypes AS (NA20838*3), SS (NA16265*C1), and SC (NA16266*B2) was obtained
(8)
Interlaboratory evaluation of high molecular weight DNA extraction methods for long-read sequencing and structural variant analysis
BMC Genomics
July 28, 2025
Alison S. Devonshire, Jordi Morata, Claire Jubin, Rui Pedro Abreu Pereira, Laura Hernandez-Hernandez, Dilek Yener, Eric Cabannes, Steven McGinn, Marc Delepine, Cédric Fund,
et al.
conditions were identical to the ones previously described by Regan et al.
Human
gDNA (catalogue no.
G3041
Promega
, Madison, WI, USA) was used as control to monitor inter-experiment variability. Sample input
(9)
Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples
Proceedings of the National Academy of Sciences
March 18, 2025
Jongwon Lim, An Bao Van, Katherine Koprowski, Matthew Wester, Enrique Valera, Rashid Bashir
synthesized by IDT. NEBuffer™ r2.1 (5 mL,
B6002S
) was purchased from
NEB
.
Human Genomic DNA
(100 µg,
G3041
) was obtained from
Promega
. DNA and Pathogens.
MRSA
DNA fragment was synthesized from IDT (Coralville,
(10)
Rapid Enzymatic Assay for Antiretroviral Drug Monitoring Using CRISPR-Cas12a-Enabled Readout
ACS Synth Biol
February 21, 2025
Maya A. Singh, Megan M. Chang, Qin Wang, Catherine Rodgers, Barry R. Lutz, Ayokunle O. Olanrewaju
and primer are listed in Table S1. REACTR with Spiked Genomic DNA 100 μg
Human
Mixed Genomic DNA (
G3041
,
Promega
) was purchased and diluted in nuclease-free water prior to experiments. Genomic DNA was diluted
(11)
Detection of a novel DNA methylation marker panel for esophageal cancer diagnosis using circulating tumor DNA
BMC Cancer
December 26, 2024
Yan Zheng, Wenqun Xing, BingWei, Xianben Liu, Guanghui Liang, Dongfeng Yuan, Ke Yang, Weizhen Wang, Dongxu Chen, Jie Ma
Universal Methylated Human DNA Standard
(
D5011
,
Zymo Research
) was used as the positive control, and
Human Genomic DNA
(
G3041
,
Promega
) was used as negative control. All
amplification
reactions were run in triplicate in 96-well
(12)
A multiplexed, allele-specific recombinase polymerase amplification assay with lateral flow readout for sickle cell disease detection
Lab on a chip
August 20, 2024
Chang MM, Natoli ME, Wilkinson AF, Tubman VN, Airewele GE, Richards-Kortum RR.
optimization was obtained from multiple sources. Mixed gender
human genomic DNA
was purchased from
Promega
(
G3041
) for genotype AA.
Human genomic DNA
was purchased from the Coriell Institute for Medical Research (Camden,
(13)
Development and validation of a ddPCR assay to detect and quantify tobacco DNA in smoke and smokeless tobacco and tobacco-free products
Heliyon
June 30, 2024
Marie-Alice Fraiture, Andrea Gobbo, Chloé Guillitte, Sophia Barhdadi, Céline Gau, Patrick Philipp, Lucas Marmin, Ugo Marchesi, Daniela Verginelli, Nina Papazova,
et al.
used (Tables 1, S3C). In addition, non-plant materials were tested, including DNA from
Homo sapiens
(
G3041
,
Promega
, USA),
Bacillus licheniformis
(LMG 7558, BCCM, Belgium),
Aspergillus niger
(IHEM 2312, BCCM,
(14)
Genome and clonal hematopoiesis stability contrasts with immune, cfDNA, mitochondrial, and telomere length changes during short duration spaceflight
Precision Clinical Medicine
April 8, 2024
J Sebastian Garcia-Medina, Karolina Sienkiewicz, S Anand Narayanan, Eliah G Overbey, Kirill Grigorev, Krista A Ryon, Marissa Burke, Jacqueline Proszynski, Braden Tierney, Caleb M Schmidt,
et al.
increase with a 30 second hold. Standard curves were prepared using
human genomic DNA
(
Promega
Cat #
G3041
) with 3-fold dilutions ranging from 50 ng to 0.617 ng in 3 μL per dilution. Negative controls included
(15)
Selection of extended CRISPR RNAs with enhanced targeting and specificity
Communications Biology
January 12, 2024
Ashley Herring-Nicholas, Hillary Dimig, Miranda R. Roesing, Eric A. Josephs
by Lazzarotto et al.5 with minor modifications. The genomic DNA (human male/female mixed,
Promega
#
G3041
)
purification
steps were carried out using the
NEB
Monarch Genomic DNA Purification Kit
. An agarose
(16)
CRISPR Screening Identifies Mechanisms of Resistance to KRASG12C and SHP2 Inhibitor Combinations in Non-Small Cell Lung Cancer
Cancer research
December 15, 2023
Prahallad A, Weiss A, Voshol H, Kerr G, Sprouffske K, Yuan T, Ruddy D, Meistertzheim M, Kazic-Legueux M, Kottarathil T,
et al
.
(300 nmol/L) Line-1 or 0.2 μL (200 nmol/L) KRAS primers, and water. Calibrator
human
gDNA (
Promega
, #
G3041
) was amplified in triplicate in a
1:4
serial dilution ranging from 0.024 ng/well to 100 ng/well.
Amplification
(17)
Extraction-free LAMP assays for generic detection of Old World Orthopoxviruses and specific detection of Mpox virus
Scientific Reports
November 30, 2023
Zhiru Li, Amit Sinha, Yinhua Zhang, Nathan Tanner, Hui-Ting Cheng, Prem Premsrirut, Clotilde K. S. Carlow
and their corresponding swab eluates samples were donated by Mirimus Inc.
Human genomic DNA
(
Promega
#
G3041
, Lot 0000531679) was used as the negative control. Viral DNA was aliquoted into small volumes and stored
(18)
A novel tailed primer nucleic acid test for detection of HPV 16, 18 and 45 DNA at the point of care
Scientific Reports
November 21, 2023
Megan M. Chang, Ariel Ma, Emilie Newsham Novak, Maria Barra, Kathryn A. Kundrod, Jane Richards Montealegre, Michael E. Scheurer, Philip E. Castle, Kathleen Schmeler, Rebecca Richards-Kortum
Target DNA dilutions were prepared in nuclease-free water on each day of experiments.
Human genomic DNA
(
G3041
,
Promega
, Madison, WI) was used as a no-target control in all RPA experiments. DNA was quantified by
(19)
A qPCR technology for direct quantification of methylation in untreated DNA
Nat Commun
August 24, 2023
Kamilla Kolding Bendixen, Maria Mindegaard, Samantha Epistolio, Giulia Dazio, Francesco Marchi, Paolo Spina, Eva C. Arnspang, Mette Soerensen, Ulf Bech Christensen, Milo Frattini,
et al.
DKO Methylated DNA cat#
D5014
(
Zymo Research
, Irvine, CA, US) and unmethylated
Human Genomic DNA
cat#
G3041
(
Promega
, Madison, WI, US), referred to as fully methylated and unmethylated DNA, respectively. A no-template
(20)
Multiplex Real-time PCR Assay for the Detection of all Chlamydia Species and Simultaneous Differentiation of C. psittaci and C. pneumoniae in Human Clinical Specimens
Annals of Laboratory Medicine
July 1, 2023
Bernard J. Wolff, Anna Gaines, Andrew B. Conley, Emily Norris, Lavanya Rishishwar, Aroon T. Chande, Eungi Yang, Maureen H. Diaz, Jonas M. Winchell
caviae),+,–,–,– CDC,
Adenovirus
,–,–,–,– CDC,
Bordetella pertussis
,–,–,–,– CDC,
Escherichia coli
,–,–,–,–
Promega
,
G3041
(
human
DNA),–,–,–,+ CDC,Group A Streptococcus,–,–,–,– CDC,Group B Streptococcus,–,–,–,– CDC,
Haemophilus influenzae
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Human Genomic DNA from Promega
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