DNA ScreenTape Analysis from Agilent Technologies

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ItemDNA ScreenTape Analysis
CompanyAgilent Technologies
Price Supplier Page View Company Product Page
Catalog NumberDNA ScreenTape Analysis
Regulatory StatusRUO

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, California 95051

United States

Phone: +1 877-424-4536

Company Profile

Website: www.agilent.com

(1) Real‐time analysis of the cancer genome and fragmentome from plasma and urine cell‐free DNA using nanopore sequencingEMBO Molecular MedicineDecember 7, 2023Ymke van der Pol, Normastuti Adhini Tantyo, Nils Evander, Anouk E Hentschel, Birgit MM Wever, Jip Ramaker, Sanne Bootsma, Marieke F Fransen, Kristiaan J Lenos, Louis Vermeulen, et al.manufacturer's instructions with 1 ml plasma input. cfDNA concentration was measured using Cell‐free DNA ScreenTape Analysis of the Agilent 4200 TapeStation System (Agilent). Cell culture Colorectal cancer cell line MDST8 was

(2) Chronic graft-versus-host disease is characterized by high levels and distinctive tissue-of-origin patterns of cell-free DNAiScienceNovember 17, 2023Yifan Pang, Temesgen E. Andargie, Moon Kyoo Jang, Hyesik Kong, Woojin Park, Thomas Hill, Neelam Redekar, Yi-Ping Fu, Desai A. Parth, Noa G. Holtzman, et al.16:27. https://doi.org/10.1186/s12896-016-0256-y. PMID: 26956612; PMCID: PMC4784296.",N/A Cell-free DNA ScreenTape Analysis,Agilent,"5067-5630, 5067–5631, 5067-5633" High Sensitivity DNA ScreenTape Analysis,Agilent,"5067-5603,

(3) Multicentric pilot study to standardize clinical whole exome sequencing (WES) for cancer patientsnpj Precision OncologyOctober 20, 2023Michael Menzel, Stephan Ossowski, Sebastian Kral, Patrick Metzger, Peter Horak, Ralf Marienfeld, Melanie Boerries, Steffen Wolter, Markus Ball, Olaf Neumann, et al.using the Illumina TruSight Oncology 500 (TSO500)-panel. DNA integrity was assessed using the Genomic DNA ScreenTape Analysis on a 4200 TapeStation System (both Agilent, Santa Clara, California). To fragment DNA to a mean fragment

(4) Sequential genomic analysis using a multisample/multiplatform approach to better define rhabdomyosarcoma progression and relapsenpj Precision OncologySeptember 20, 2023Henry de Traux de Wardin, Josephine K. Dermawan, Marie-Sophie Merlin, Leonard H. Wexler, Daniel Orbach, Fabio Vanoli, Gudrun Schleiermacher, Birgit Geoerger, Stelly Ballet, Delphine Guillemot, et al.all samples were then processed and barcoded similarly, quantified, and then qualified with the D1000 DNA ScreenTape analysis kit according to manufacturer protocols (Agilent®). Tumor samples molecular analyses Detailed descriptions

(5) High frequency of WNT-activated medulloblastomas with CTNNB1 wild type suggests a higher proportion of hereditary cases in a Latin-Iberian populationFrontiers in OncologySeptember 4, 2023Daniel Antunes Moreno, Murilo Bonatelli, Augusto Perazzolo Antoniazzi, Flávia Escremim de Paula, Leticia Ferro Leal, Felipe Antônio de Oliveira Garcia, André Escremim de Paula, Gustavo Ramos Teixeira, Iara Viana Vidigal Santana, Fabiano Saggioro, et al.HS fluorimetric assays (Life Technologies, USA). Quality control of fragment size was assessed using DNA ScreenTape analysis (4150 TapeStation System, Agilent). Sequencing was achieved with the final library concentration of

(6) Protocol optimization of a targeted sequencing panel for genomic profiling of bronchoalveolar lavage fluid in lung cancerCancer MedicineSeptember 1, 2023Cassandra L. Sather, Pamela Yang, Chaomei Zhang, Matthew P. Fitzgibbon, Michelle Fournier, Eric Toloza, Amit Tandon, Matthew Schabath, Sean Yoder, Viswam S. Nair(Thermo Fisher Scientific). Cell‐free BAL DNA and plasma cfDNA were quantified and checked for quality using the Cell‐Free DNA ScreenTape Analysis on the 4200 TapeStation instrument (Agilent Technologies). Fragmentation by sonication (Covaris) was

(7) The SWI/SNF complex member SMARCB1 supports lineage fidelity in kidney canceriScienceAugust 18, 2023Wesolowski L, Ge J, Castillon L, Sesia D, Dyas A, Hirosue S, Caraffini V, Warren AY, Rodrigues P, Ciriello G, et al.reagents,Thermo-Fisher,4352405 Taqman probe PAX8,Thermo-Fisher,Hs00247586_m1 Taqman probe TBP,Thermo-Fisher,Hs00427620_m1 DNA ScreenTape Analysis: D1000 reagents,Agilent,5067-5583 NEBNext PCR master mix,NEB,M0541S AMPureXP,Agencourt,A63880 KAPA HiFi HotStart ReadyMix

(8) BAP1 and PTEN mutations shape the immunological landscape of clear cell renal cell carcinoma and reveal the intertumoral heterogeneity of T cell suppression: a proof-of-concept studyCancer Immunology, ImmunotherapyJune 1, 2023Jana Friedhoff, Felix Schneider, Christina Jurcic, Volker Endris, Martina Kirchner, Angela Sun, Iulia Bolnavu, Laura Pohl, Miriam Teroerde, Maximilian Kippenberger, et al.preparation was performed as previously described [15]. DNA integrity was assessed using the Genomic DNA ScreenTape Analysis on a 4150 TapeStation System (Agilent Technologies, Santa Clara, CA, USA). To fragment DNA to a length

(9) Seascape genomics and mitogenomic phylogeography of the sailfish (Istiophorus platypterus)Genome Biology and EvolutionApril 6, 2023Bruno Lopes da Silva da Silva Ferrette, Raphael T F Coimbra, Sven Winter, Menno J De Jong, Samuel Mackey Williams, Rui Coelho, Daniela Rosa, Matheus Marcos Rotundo, Freddy Arocha, Bruno Leite Mourato, et al.the final library was assessed on the TapeStation System (Agilent Technologies©) using the Genomic DNA ScreenTape Analysis kit and the Invitrogen™ Qubit™ Fluorometer using the Qubit™ dsDNA high sensitivity (HS) assay kit (Thermo

(10) Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gutFrontiers in MicrobiologyMarch 20, 2023Francesca Algieri, Nina Tanaskovic, Cindy Cardenas Rincon, Elisabetta Notario, Daniele Braga, Graziano Pesole, Roberto Rusconi, Giuseppe Penna, Maria Rescignoprotocol. DNA quality control was performed with the Agilent 4,200 Tape Station system using the Genomic DNA ScreenTape analysis kit (Agilent, Santa Clara, CA, United States), only DNAs having a DIN > 6.5 were used for library preparation. Analysis

(11) Antimicrobial Resistance in Vaginal Bacteria in Inseminated MaresPathogensFebruary 24, 2023Pongpreecha Malaluang, Elin Wilén, Sara Frosth, Johanna F. Lindahl, Ingrid Hansson, Jane M. MorrellDNA Library Preparation Kit (Illumina, San Diego, CA, USA), and the quality was assessed by the HS DNA ScreenTape Analysis D1000 (Agilent Technologies, Inc., Santa Clara, CA, USA) on a 4150 TapeStation (Agilent Technologies,

(12) Combined low-pass whole genome and targeted sequencing in liquid biopsies for pediatric solid tumorsnpj Precision OncologyFebruary 20, 2023Eirini Christodoulou, Venkata Yellapantula, Katrina O’Halloran, Liya Xu, Jesse L. Berry, Jennifer A. Cotter, Anya Zdanowicz, Leo Mascarenhas, James F. Amatruda, Dejerianne Ostrow, et al.abundance and quality of cfDNA in the extracted samples were assessed using a bioanalyzer Cell-free DNA ScreenTape analysis (Agilent, Santa Clara, CA) and Quantus™ Fluorometer (Promega, Madison, WI). Library preparation and

(13) Assessing the pathogenicity of BRCA1/2 variants of unknown significance: Relevance and challenges for breast cancer precision medicineFrontiers in OncologyJanuary 18, 2023Elisa De Paolis, Ida Paris, Bruno Tilocca, Paola Roncada, Laura Foca, Giordana Tiberi, Tatiana D’Angelo, Francesco Pavese, Margherita Muratore, Luisa Carbognin, et al.dsDNA HS fluorimetric assays (Life Technologies). Quality control of fragments size was assessed using DNA ScreenTape analysis (4200 TapeStation system, Agilent Technologies). Sequencing run was performed in paired-ends reads mode

(14) In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in DrosophilaSTAR ProtocDecember 16, 2022Jocelyn L.Y. Tang, Robert Krautz, Oriol LlorÃ -Batlle, Anna E. Hakes, Paul M. Fox, Andrea H. BrandHyperladder,Bioline,Cat#BIO-33057 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Genomic DNA ScreenTape Analysis Reagents,Agilent,Cat#5067-5366 QiaAmp DNA Micro Kit,QIAGEN,Cat#56304 Qiagen PCR Purification Kit,QIAGEN,Cat#28106 "Qubit dsDNA HS Assay Kit

(15) Diagnostic analysis of the highly complex OPN1LW/OPN1MW gene cluster using long-read sequencing and MLPANPJ Genom MedNovember 9, 2022Lonneke Haer-Wigman, Amber den Ouden, Maria M. van Genderen, Hester Y. Kroes, Joke Verheij, Dzenita Smailhodzic, Attje S. Hoekstra, Raymon Vijzelaar, Jan Blom, Ronny Derks, et al.followed by a final elongation step of 10 min at 65 °C. All amplicons were checked on agarose gel or DNA ScreenTape Analysis (TapeStation, Agilent). Library preparation LR amplicons were purified by AMPure PB beads (Pacific

(16) Precision oncology for intrahepatic cholangiocarcinoma in clinical practiceBritish Journal of CancerNovember 1, 2022Aurelie Tomczak, Christoph Springfeld, Michael T. Dill, De-Hua Chang, Daniel Kazdal, Ursula Wagner, Arianeb Mehrabi, Antje Brockschmidt, Tom Luedde, Patrick Naumann, et al.Diego, CA, USA) library preparation, the DNA integrity status of a sample was determined by Genomic DNA ScreenTape Analysis using a 4150 TapeStation System (both Agilent, Santa Clara, CA, USA). 80 ng of total DNA of each sample

(17) Rapid and Efficient Extraction of Cell-Free DNA Using Homobifunctional CrosslinkersBiomedicinesAugust 4, 2022HyeonAh Seong, Junsoo Park, Minju Bae, Sehyun Shinextraction, as well as all other chemicals, were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell-free DNA ScreenTape Analysis and the 4200 TapeStation System used for cfDNA analysis were purchased from Agilent Technologies (Santa

(18) DiMeLo-seq: a long-read, single-molecule method for mapping proteinâ€“DNA interactions genome wideNature MethodsJune 1, 2022Nicolas Altemose, Annie Maslan, Owen K. Smith, Kousik Sundararajan, Rachel R. Brown, Reet Mishra, Angela M. Detweiler, Norma Neff, Karen H. Miga, Aaron F. Straight, et al.(100-938-900) with 1 μg input to library preparation. DNA size was determined using the TapeStation Genomic DNA ScreenTape Analysis (Agilent 5067–5365 & 5067–5366) and DNA quantification was performed using the Qubit (Invitrogen Q32853). Primer

(19) Immunogenicity and therapeutic targeting of a public neoantigen derived from mutated PIK3CANature medicineMay 1, 2022Chandran SS, Ma J, Klatt MG, Dündar F, Bandlamudi C, Razavi P, Wen HY, Weigelt B, Zumbo P, Fu SN, et al.PCR amplification. cDNA quality control and quantification were determined by High Sensitivity D5000 DNA ScreenTape analysis (Agilent Technologies) and Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). Fifty nanograms of cDNA

(20) High molecular weight DNA extraction methods lead to high quality filamentous ascomycete fungal genome assemblies using Oxford Nanopore sequencingMicrobial GenomicsApril 19, 2022Celine Petersen, Trine Sørensen, Klaus R. Westphal, Lavinia I. Fechete, Teis E. Sondergaard, Jens L. Sørensen, Kåre L. Nielsen(ThermoFisher), Qubit 3.0 (Invitrogen) with Qubit dsDNA HS Assay Kit, and 2200 TapeStation (Agilent) with Genomic DNA ScreenTape Analysis according to the manufacturers’ instructions. Removal of small fragments from the extracted DNA Circulomics

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