Fig 1: Light microscopic appearance of hepatocytes isolated with the gentleMACS Perfusion Technology. Primary hepatocytes were isolated from a 3-month-old male mouse and seeded in HepatoZYME-SFM medium (#17705021, ThermoFisher, Dreieich, Germany). The medium was changed to Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum, 2 mM L-glutamine, 100 IU/mL penicillin, and 100 µg/mL streptomycin (all from ThermoFisher) two hours after initial plating. Pictures were taken at indicated times and magnifications.