M13K07 Helper Phage from New England Biolabs

Product Specs
ItemM13K07 Helper Phage
CompanyNew England Biolabs
Price $122.00 Inquire
Catalog NumberN0315S
Quantity1.8 ml
ApplicationsProteomics & Glycomics
Cell Type (standardized)Microbial Cells
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Conservation of Long G4-rich (LG4) genomic enhancer regulationsBMC GenomicsAugust 7, 2026Michael H. Shaw, Jeffrey D. DeMeis, Claire A. Arnold, Makala R. Cox, Tien C. Duong, Kyle A. Gaviria, Grace K. McDavid, Jose M. Villegas, Margaret L. Weimer, Suhas S. Patil, et al.LB then grown at 37 °C for 4 h at 225 RPM in an orbital shaker. M13KO7 helper phage (NEB; Cat. No. N0315S) was then added to each [1 × 108 pfu/ml] and incubated at 37 °C for 1.5 h at 225 RPM then kanamycin

(2) Directed evolution of chlorotoxin enhances MMP-2 recognition and improves CAR-T-cell activity in glioblastoma models in vitroFront Bioeng BiotechnolJuly 20, 2026Anna Hajdara, Árpád Szöőr, József Murányi, Attila Brunyánszki, Gábor Z. Rácz, Daniel Cioca, Ambrus Gordos, Natali Bata, Noémi Nagy, Imre Fedorcsák, et al.electrocompetent cells (60514-1-LU, BioCat), then the cell culture was infected with M13KO7 helper phage (N0315S, New England Biolabs). Phage propagation and preparation followed a standard protocol (Tonikian et al.,

(3) Integrating fab phage and yeast display through seamless transfer of paired heavy- and light-chain libraries for efficient antibody discoveryAntibody TherapeuticsJune 29, 2026Wei Zhang, Hao Ye, Xingrun Zhang, Cheng Liaomedium (100 μg/ml ampicillin, 2% glucose) at 37°C until OD600 reached 0.4–0.6. M13KO7 helper phage (NEB, N0315S) was added, and incubated at 37°C for 30 min without shaking, followed by 1 h with shaking. Cells were

(4) Generation of HBV cccDNA using single-stranded M13 phage DNA for authentic minichromosome functionalityJournal of VirologyJune 23, 2026Yumeng Li, Ting Hua, Menghan Hao, Liman Chen, Wenjun Huang, Zhong Fang, Asha Ashuo, Yaming Li, Zhigang Yi, Hongzhou Gu, et al.media and incubated at 37°C until the OD600 reached 0.4–0.5. At this point, M13KO7 helper phage (NEB, N0315S) was added at a multiplicity of infection (MOI) of 1-10, and the culture was shaken at 37°C and 200–220

(5) Single-domain antibody inhibitors target the coiled coil arms of the Bacillus subtilis SMC complexElifeMay 19, 2026Ophélie J Gosselin, Michael Taschner, Lea M Huber-Hürlimann, Markus A Seeger, Stephan GruberCenter,1A700,"BSG1001, trpC2" Recombinant DNA reagent,M13KO7 helper phage,New England Biolabs,Cat# N0315S,For phage production Recombinant DNA reagent,Plasmid pSBinit,AddGene,RRID:Addgene_110100,Vector for

(6) A Potent Single-Domain Antibody Targeting LAG-3 for Efficient Tumor ImmunotherapyCurrent issues in molecular biologyMay 4, 2026Dong M, Li W, Wang T, Li M, Zhang J, Liu X.containing ampicillin (Beyotime, Shanghai, China, Cat:ST005) and helper phages (NEB, Ipswich, MA, USA, Cat: N0315S). Individual phage clones were then screened for specific LAG-3 binding by enzyme-linked immunosorbent assay

(7) Cell-type-targeted mitochondrial transplantation rescues cell degenerationNatureMay 1, 2026Ayupov T, Moreno-Juan V, Curtoni S, Fratzl A, Sharma U, Posada-Céspedes S, Ratiu R, Morikawa R, Meyer AG, Pezzoli M, et al.(Biosearch Technologies, 60502-2). Transformed cells were superinfected with M13K07 helper phage (NEB, N0315S) and phagemids were purified from culture supernatant. Phagemids were cycled through 3−4 rounds of phage display

(8) A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation libraryNucleic Acids ResApril 13, 2026Yiwen Zhang, Hang Zhou, Xiangxiang Xu, Kaining Zhang, Guozhong Huang, Feiyu Fan, Dongdong Zhao, Qingyan Li, Xinna Zhu, Xueli Zhangat 37°C, 250 rpm until just slightly turbid (OD600 < 0.05). Add 50 µl M13KO7 (New England Biolabs, N0315S) helper phage [final concentration of 1 × 108 plaque-forming unit (PFU)/ml] and grow overnight (14–18

(9) Generation of Nano-Antibodies Targeting the SNMP1 Membrane Receptor of Spodoptera littoralis Using Extracellular VesiclesJ Extracell BiolApril 7, 2026Alexis Dogliani, Laura Fradale, Céline Valeille, Christian Cambillau, Emmanuelle Jacquin-Joly, Alain Roussel, Josiane Kassis-Sahyoun#60502‐02) via electroporation, followed by culturing in the presence of the helper phage M13KO7 (NEB, #N0315S) to construct the Nab phage display library. Phages displaying Nabs on their surface were precipitated

(10) A novel bispecific nanobody protects mice against RSV infection via intranasal administrationJournal of VirologyDecember 23, 2025Min Zhang, Liuxing Qin, Raoqing Guo, Jinwei Li, Si Huang, Chen Sheng, Yijia Xiang(Weidi, DE1055). Phage display libraries were generated by superinfection with M13KO7 helper phage (NEB, N0315S), followed by phage precipitation using 20% PEG/2.5 M NaCl. To isolate high-affinity neutralizing nanobodies

(11) Nanofilament immunotherapy induces potent antitumor vaccine responsesJournal for immunotherapy of cancerAugust 31, 2025Neil K, Génier S, Poisson MÈ, Douchin J, Giguère H, Daniel L, Gonzalez MF, Huang S, Trinh VQ, Tai LH, et al.ranging from 1×107 to 5×109 bacteriophage particles/mL was prepared using M13K07 helper phage (NEB, N0315S) diluted in PBS. Bound phages were detected using an anti-M13/fd/f1-HRP antibody conjugate (Progen,

(12) Rationally designed self-assembled peptide nanofibers provoke robust humoral immunity against nervous necrosis virusJournal of VirologyAugust 19, 2025Chen Zhang, Yong-Can Zhou, Wen-Ye Song, Xin-Xin Liu, Hai-Hua Peng, Yun SunProgeny phage production was initiated by infecting transformed TG1 cells with helper phage M13K07 (NEB, N0315S). Cell-secreted recombinant phage particles displaying the fusion protein were precipitated using PEG/NaCl

(13) Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodiesSci RepMay 26, 2025Pavana Suresh, Sun Zhen-Yu Jim, Ishani Sahay, Allison Y. Tang, Charlotte Wijne, Jin Gan, Novalia Pishesha, Jose L. Avalos, Hidde Ploeghelectroporation. Phage display library generation TG1 library cells were infected with helper phage M13KO7 (NEB, N0315S) at a 1:100 ratio (cells:phage) and cultured overnight at 30 °C, 220 rpm in 2YT media supplemented with

(14) A Two-Component Cocktail of Engineered E Domain III Nanoparticles Elicits Broadly Neutralizing Antibody Responses against Dengue virus in MiceiScienceApril 29, 2025Margarette C. Mariano, Helen S. Jung, Olivia Vergnolle, Keith Haskell, Lamount R. Evanson, Gregory Quevedo, Julia C. Frei, Karen Tong, Larissa B. Thackray, Michael S. Diamond, et al.University),N/A DENV 4 (1036),Gift from M.S. Diamond (Washington University),N/A M13KO7 Helper phage,NEB,Cat# N0315S E. coli CJ236,Lucigen,Cat# 06701-2 E. coli SS320,Lucigen,Cat# 60512-2 E. coli XL-1 Blue,Agilent,Cat#

(15) Optimization of synthetic human VH affinity and solubility through in vitro affinity maturation and minimal camelizationProtein SciApril 22, 2025Kasandra Bélanger, Cunle Wu, Traian Sulea, Henk van Faassen, Deborah Callaghan, Annie Aubry, Marc Sasseville, Greg Hussack, Jamshid Tanha(Sigma, Cat#A0170 and Thermo Fisher Scientific, Cat#A18818, respectively). M13K07 helper phage (Cat#N0315S), Q5® Hot Start High Fidelity DNA Polymerase (Cat#M0493L) and dNTP mix (Cat#N0447L) were purchased from

(16) A nanobody that binds to the backside of the ubiquitin conjugating enzyme Ube2G2 differentially affects interactions with its partner E3 LigasesCommun BiolApril 16, 2025Pavana Suresh, Charlotte Wijne, Zhen-Yu J. Sun, Nanette Becht, Ishani Sahay, Novalia Pishesha, Hidde Ploegh200123). Phage display library was produced by infecting TG1 library with helper phage M13KO7 (NEB, N0315S) and grown O/N in 2YT media (18 g/L tryptone, 10 g/L yeast extract, 5 g/L NaCl) with Ampicillin (100

(17) Mapping the extracellular molecular architecture of the pAg-signaling complex with α-Butyrophilin antibodiesSci RepApril 9, 2025Amrita Ramesh, Sobhan Roy, Tomasz Slezak, James Fuller, Hortencia Graves, Murad R. Mamedov, Alexander Marson, Anthony A. Kossiakoff, Erin J. Adams,New England biolabs,Cat#C2527H XL-1 E. coli,Agilent/stratagene,Cat#200249 M13K07 helper phage,New England biolabs,N0315S "Chemicals, peptides and recombinant proteins","Chemicals, peptides and recombinant proteins","Chemicals,

(18) Development of chemokine network inhibitors using combinatorial saturation mutagenesisCommun BiolApril 3, 2025Jhanna Kryukova, Serena Vales, Megan Payne, Gintare Smagurauskaite, Soumyanetra Chandra, Charlie J. Clark, Graham Davies, Shoumo Bhattacharya950 μL recovery media and transferred to 50 mL Falcon tubes. 100 μL 1011 CFU/mL M13KO7 helper phage (N0315S, NEB) was added, and tubes shaken at 37 °C, 220 RPM, 1 h. 1 mL recovery culture was added to 2YT medium

(19) Engineered protein G variants for multifunctional antibody-based assembliesProtein SciJanuary 25, 2025Tomasz Slezak, Kelly M. O'Leary, Jinyang Li, Ahmed Rohaim, Elena K. Davydova, Anthony A. Kossiakofftemperature. Then, media containing 100 μg/mL ampicillin and 109 p.f.u./mL of M13K07 helper phage (NEB, Cat # N0315S) was added for overnight phage amplification at 37°C. For all subsequent rounds, the amplified phage

(20) Isolation and Characterization of the First Antigen-Specific EGFRvIII vNAR from Freshwater Stingray (Potamotrygon spp.) as a Drug Carrier in Glioblastoma Cancer CellsInternational Journal of Molecular SciencesJanuary 21, 2025Alejandro Manzanares-Guzmán, Andrea C. Alfonseca-Ladrón de Guevara, Elia Reza-Escobar, Mirna Burciaga-Flores, Alejandro Canales-Aguirre, Hugo Esquivel-Solís, Pavel H. Lugo-Fabres, Tanya A. Camacho-Villegascontaining SB medium (198 mL) supplemented with ampicillin (100 µg/µL) and a helper phage M13K07 (2 mL; N0315S, New England Biolabs, Ipswich, MA, USA). The culture was incubated for 2 h under identical conditions

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