HeLa S3 from ATCC

Product Specs
ItemHeLa S3
CompanyATCC
Price $431.00Get Pricing Get Quote for this Product
Catalog NumberCCL-2.2
ApplicationsThis cell line is a suitable transfection host. The HeLa S3 clone has been very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition, and plaque-forming ability
TypeCell Biology
SpeciesHomo sapiens
TissueUterus, Cervix
Cell LineHeLa S3
SourceHuman / Cervix
DiseaseAdenocarcinoma
StorageLiquid Nitrogen Vapor Phase / Complete Growth Medium Supplemented with 5% (v/v) DMSO
ShippedFrozen
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Cervix
Age31 years

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) The C16orf87 protein is a subunit of the MIER corepressor complex controlling embryonic development and cell migrationSci RepApril 30, 2026Tanel Punga, Mårten Larsson, Endrina Mujica, Fabio Rabelo Melo, Christoph Metzendorf, Hanqing Zhang, Dandan Wang, Marcel den Hoed, Leif Andersson, Débora Parrineskeletal muscle cell line C2C12 (ATCC, CRL-1772), and human cervical cancer cell line HeLa S3 (ATCC, CCL-2.2) were used in this study. The HeLa S3 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM,

(2) An oncolytic vaccinia virus encoding CD47 nanobody potentiates antitumor immunity in multiple myelomaiScienceApril 17, 2026Lingli Pan, Xiaomeng Zhu, Jiaqing Zhang, Qingyao Zhong, Hui Wu, Weidong Sun, Yongming Xia, Shibing Wang, Xiangmin TongP3/NSI/1-Ag4-1 (NS-1),ATCC,Cat# TIB-18 Sp2/0-Ag14 (SP2/0),ATCC,Cat# CRL-1581 HEK293,ATCC,Cat# CRL-1573 HeLa-S3,ATCC,Cat# CCL-2.2 Vero,ATCC,Cat# CCL-81 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models

(3) Antibody Binding and Neutralizing Targets within the Predicted Structure of the Poxvirus Multiprotein Entry-Fusion ComplexbioRxivApril 12, 2026Huibin Yu, Wolfgang Resch, Catherine A. Cotter, Wei Xiao, Tase Karamanolis, Ahmed A. Belghith, Maxinne A. Ignacio, Patricia L. Earl, Gary H. Cohen, Bernard Mosshumidity. Adherent HEK293T cells were maintained in DMEM (Gibco) with 10% FBS at 37°C. Hela S3 cells (ATCC, CCL-2.2) were cultured in spinnermodified minimum essential medium (SMEM) with 1% Non-Essential Amino Acids,

(4) Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformationeLifeFebruary 9, 2026Takashi Hotta, Morgan L Pimm, Ezekiel C Thomas, Yang Yue, Patrick DeLear, Lynne Blasius, Michael A Cianfrocco, Morgan E DeSantis, Ryota Horiuchi, Takumi Higaki, et al.drug",Penicillin-Streptomycin,"Gibco, Thermo Fisher",Cat# 15140122, Cell line (Homo sapiens),"HeLa S3 cells, female",ATCC (CCL-2.2),RRID:CVCL_0058, Other,Dulbecco’s modified Eagle medium (DMEM),"Gibco, Thermo Fisher",Cat# 11965092, Other,Leibovitz’s L-15 Medium

(5) Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformationElifeFebruary 9, 2026Takashi Hotta, Morgan L Pimm, Ezekiel C Thomas, Yang Yue, Patrick DeLear, Lynne Blasius, Michael A Cianfrocco, Morgan E DeSantis, Ryota Horiuchi, Takumi Higaki, et al.drug",Penicillin-Streptomycin,"Gibco, Thermo Fisher",Cat# 15140122, Cell line (Homo sapiens),"HeLa S3 cells, female",ATCC (CCL-2.2),RRID:CVCL_0058, Other,Dulbecco’s modified Eagle medium (DMEM),"Gibco, Thermo Fisher",Cat# 11965092, Other,Leibovitz’s L-15 Medium

(6) Integrative Single-Cell Epigenomic Atlas Annotates the Regulatory Genome of the Adult Mouse BrainbioRxivFebruary 7, 2026Zhaoning Wang, Songpeng Zu, Ethan J. Armand, Timothy H. Loe, Jonathan A. Rink, Wanying Wu, Yang Xie, Lei Chang, Chenxu Zhu, Nicholas D. Johnson, et al.(30–70%), and with ad libitum access to food and water. Cell lines and cell culture HeLa S3 (ATCC, CCL-2.2) cells were cultured in Dulbecco’s Modified Eagle Medium (ThermoFisher Scientific, 11995073) supplemented

(7) How to Unmask an Unknown: The Restriction-Modification System MhoVII of Mycoplasma hominis Expresses Two Complementary Methylation Activities in One EnzymeInt J Mol SciFebruary 5, 2026Lars Vogelgsang, Dana Bäcker, Sebastian Alexander Scharf, Azlan Nisar, Alexander T. Dilthey, Birgit HenrichOxford, UK). 5.7. Hela Cell Infection Assay The human cervical carcinoma cell line HeLa S3 (ATCC CCL-2.2) that was uninfected or infected by M. hominis strains FBG or SS10 was cultivated in DMEM, and 10 million

(8) Differential nucleosome organization in human interphase and metaphase chromosomesMolecular Systems BiologyFebruary 2, 2026Keren Li, Irem Unlu, Yiren Tu, Lilien N Voong, Yanyan Lu, Brody Kendall, Xiaotian Ma, Sin Lei Pui, Meng Tao, Ji-Ping Wang, et al.centrifugation. Generation of H4S47C-expressing HeLa S3 cell lines Parental human HeLa S3 cells (ATCC CCL-2.2) were recently authenticated and tested for mycoplasma contamination and was used to generate H4S47C-expressing

(9) Enhancing gene therapy vectors manufacturing: CEBPA as a master regulator of rAAV biogenesisJournal of Biological EngineeringJanuary 20, 2026Filipa Moura, Mariana Antunes, Ricardo Correia, António Roldão, Patrícia Gomes-Alves, Paula Marques Alves, Jose M. Escandellclinical and commercial gene therapy applications. Methods Cell culture conditions HeLaS3 (ATCC CCL-2.2) derived rAAV PCLs were generated as described in [8]. Cells were routinely sub-cultured at 0.3x106

(10) Streamlined rAAV HeLaS3 producer cell line generation via GS selectionScientific ReportsJanuary 9, 2026Mariana Antunes, Filipa Moura, Ivy Rose Sebastian, Paula Alves, Patrícia Gomes-Alves, Jose M. Escandellclinical demand19. Materials and methods Cell culture HeLaS3 cell line was acquired from ATCC (CCL-2.2) and maintained in adherent culture at 37 ºC and in a 5 % CO2 atmosphere, in Dulbecco’s Modified Eagle’s Medium (DMEM

(11) FLARE: A Flow Cytometry–Based Fluorescent Assay for Measuring HSV-1 Nuclear EgressBIO-PROTOCOLJanuary 5, 2026Bing Dai, Lucas Polack, Samantha Moores, Ariana C. Calderón-Zavala, Ekaterina E. Heldweincontrolling herpesvirus nuclear egress. Materials and reagents Biological materials 1. HeLa cells (ATCC, CCL2) 2. Hep-2 cells (ATCC, CCL23) 3. Vero Cells (ATCC, CCL-81) 4. HSV-1-tdTomato [strain HSV-1 F GS3217, a

(12) FLARE: A Flow Cytometry–Based Fluorescent Assay for Measuring HSV-1 Nuclear EgressBio ProtocJanuary 5, 2026Bing Dai, Lucas Polack, Samantha Moores, Ariana C. Calderon-Zavala, Ekaterina E. Heldweincontrolling herpesvirus nuclear egress. Materials and reagents Biological materials 1. HeLa cells (ATCC, CCL2) 2. Hep-2 cells (ATCC, CCL23) 3. Vero Cells (ATCC, CCL-81) 4. HSV-1-tdTomato [strain HSV-1 F GS3217, a

(13) Automated chromatin profiling with spa-ChIP-seq uncovers the impacts of condition variationsGenome ResearchJanuary 5, 2026Yuwei Cao, Lauren Patel, Lauren Alcoser, Eric Mendenhall, Christopher Benner, Sven Heinz, Alon Gorenpotentially advance diagnostic approaches. METHODS Cell culture and fixation (crosslinking) HeLa-S3 (ATCC CCL-2.2™), HEK293T (CRL-3216™), and HEK293T-BRD4-FKBP12F36V-2xHA adherent cells were cultured in Dulbecco’s

(14) The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infectionScience advancesOctober 24, 2025Woo TT, Takeo Y, Harwood MC, Houck ET, DiMaio D, Tsai B.Cell culture HeLa [American Type Culture Collection (ATCC), catalog no. CCL-2], HeLa-S3 (ATCC, catalog no. CCL-2.2), and SiHa (ATCC, catalog no. HTB-35) cells were obtained from ATCC. HaCaT cells were purchased from

(15) Preparation of Chromatin Fragments From Human Cells for Cryo-EM AnalysisBIO-PROTOCOLOctober 20, 2025Suguru Hatazawa, Yoshimasa Takizawa, Hitoshi Kurumizakadiverse biological contexts. Materials and reagents Biological materials 1. HeLa S3 cells (ATCC, CCL-2.2) Reagents 1. Dulbecco’s modified Eagle medium (DMEM) (Nacalai, catalog number: 08458-16) 2. Fetal bovine

(16) Preparation of Chromatin Fragments From Human Cells for Cryo-EM AnalysisBio ProtocOctober 20, 2025Suguru Hatazawa, Yoshimasa Takizawa, Hitoshi Kurumizakadiverse biological contexts. Materials and reagents Biological materials 1. HeLa S3 cells (ATCC, CCL-2.2) Reagents 1. Dulbecco’s modified Eagle medium (DMEM) (Nacalai, catalog number: 08458-16) 2. Fetal bovine

(17) Massively parallel assay of human splice variants reveals cis-regulatory drivers of disease-associated and cell type-specific splicing regulationbioRxivOctober 16, 2025Samantha E. Koplik, Angela M Yu, Madelyn R. Shelby, Gabriel C. Fonseca, Charles M. Roco, Yue Zhang, Nicholas Bogard, Alex K. Sabo, Alexander B. Rosenberg, Johannes Linder, et al.Cell Culture and Transfection HEK-293 cells (ATCC, CRL-1573), MCF7 cells (ATCC, HTB22), and HeLa cells (ATCC, CCL-2.2) were cultured in DMEM (Gibco). K-562 cells (ATCC, CCL-243) were cultured in RPMI (Gibco). HCM3 cells

(18) Real-time visualization of reconstituted transcription reveals RNAPII activation mechanisms at single promotersCell reportsSeptember 23, 2025Palacio M, Taatjes DJ.study participant details,Experimental model and study participant details ,, HeLa S3 cells,ATCC,CCL-2.2 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Pierce™ RNA 3′ End Biotinylation Kit

(19) Protocol for monitoring mRNA translation and degradation in human cell-free lysatesSTAR ProtocolsSeptember 19, 2025Nino Schwaller, Evangelos D. Karousislines,Experimental models: Cell lines,Experimental models: Cell lines ,, HeLa S3 cells,ATCC,Cat. No. CCL-2.2 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, p200 5′UTR hRLuc for northern blotting

(20) Structural modifications of toxyloxanthone C and macluraxanthone isolated from Maclura cochinchinensis: cytotoxicity, antibacterial activity, and in silico studiesRSC AdvancesSeptember 11, 2025Chaiwat Linphosan, Waranya Klangsawad, Jantana Yahuafai, Jinda Jandaruang, Trinop Promgool, Siripit Pitchuanchom, Jakkapat Paluka, Sophon Boonlue, Kitisak Poopasit, Kwanjai Kanokmedhakul, et al.cells were purchased from ATCC. Cytotoxicity assays against human cervical carcinoma (HelaS3; ATCC CCL2-2), human hepatocellular carcinoma (HepG2; ATCC HB-8065), human lung carcinoma (A549; ATCC CCL-185), and

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HeLa S3 from ATCC

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