F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) from ATCC

Product Specs
ItemF-12K Medium (Kaighn's Modification of Ham's F-12 Medium)
CompanyATCC
Price $18.00Supplier Page View Company Product Page
Catalog Number30-2004
ApplicationsF-12K Medium (Kaighn's Modification of Ham's F-12 Medium) contains 2 mM L-glutamine and 1500 mg/L sodium bicarbonate
TypeMedia Sera Reagents
Volume500 mL

Specifications/Features

Contains 2 mM L-glutamine and 1500 mg/L sodium bicarbonate

Contains:
Contains 2 mM L-glutamine and 1500 mg/L sodium bicarbonate

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Coordination of nuclear RNA processing by speckle-localized kinase TAOK2Genes DevAugust 3, 2026Bridget E Begg, Matthew A Tracey, Shengyan Gao, Svetlana Earnest, Mohd Altaf Najar, George M Burslem, Melanie H Cobb, Beatriz M A Fontoura, Kristen W Lynch1999). Two confluent 10 cm dishes of A549 cells grown in F12K medium supplemented with 10% FBS (ATCC 30–2004) were washed in PBS and lysed on ice with a 27 gauge syringe in 0.25 ml of lysis buffer containing 50

(2) Orforglipron, a Small-Molecule Glucagon-like Peptide-1 Receptor Agonist (GLP-1RA), Has Neuroprotective and Anti-Inflammatory EffectsCellsJuly 21, 2026Yazhou Li, Elliot J. Glotfelty, Pathik Parekh, Buyandelger Batsaikhan, Weiming Luo, Shelley N. Jackson, Brandon K. Harvey, Nigel H. GreigEagle’s Minimum Essential Medium (EMEM; ATCC® 30-2003™, Manassas, VA, USA) and Ham’s F12-K (Kaighn’s) Medium (ATCC® 30-2004™), supplemented with 10% heat-inactivated fetal bovine serum (Gibco™ Cat#10082147, Invitrogen, Carlsbad,

(3) VEGFR2 dimerization kinetics on the cell surface determined by microscopy and mathematical modelingBiophysical JournalJuly 21, 2026Jaime Guerrero, Zachariah Malik, Fnu Bilal, Soma Jana, Aparajita Dasgupta, Khuloud JaqamanChinese Hamster Ovary (CHO-K1, ATCC, CCL-61, Manassas, VA) cells were grown in F-12K culture medium (ATCC, 30–2004) supplemented with fetal bovine serum (FBS, Thermo Fisher Scientific, A56697–01, Waltham, MA) added

(4) Oxyresveratrol Suppresses EGF-Induced AKT Phosphorylation and Reduces Cellular Fitness While Promoting Apoptosis in EGFR-Wild-Type Non-Small Cell Lung Cancer Cells Under EGF StimulationInt J Mol SciJuly 18, 2026Wutigri Nimlamool, Jatuporn Polhiran, Nitchakarn Phimthong, Saranyapin Potikanond, Nitwara WikanCollection (ATCC) (Manassas, VA, USA). A549 cells were cultured in ATCC-formulated F-12K medium (ATCC 30-2004) (ATCC) supplemented with 10% fetal bovine serum (FBS) (Gibco, New York, NY, USA) and 1% penicillin/streptomycin

(5) Vascular endothelial growth factor receptors 1 and 3 mediate placental trophoblast leptin production in preeclampsia, inducing vascular dysfunctionArterioscler Thromb Vasc BiolJuly 9, 2026Mona Elgazzaz, Safia Ogbi, Desmond Moronge, Elisabeth Mellott, Gibson Cooper, Kristin Backer, Amalia Brawley, Gabrielle Hernendez, Joanna Hitchings, Luis Valesquez Zarate, et al.cultured in a complete medium (F12K media, 10 % fetal bovine serum, and 1 % penicillin streptomycin (#302004, ATCC, USA). HUVECs (# CC-2519, Lonza, Switzerland) were cultured in EBM2 media (#CC-3156, Lonza, Switzerland).

(6) Solvent alternation of the antimicrobial, antioxidant, anticancer activities and chemical profiles of Trametes hirsuta (Wulfen) LloydBMC MicrobiolJuly 1, 2026Yaser Mahmoud Ahmed, Amer Morsy Abdelaziz, Hussein H. El-Sheikh, Ahmed M. Younissodium pyruvate, sodium bicarbonate, FBS, and Earle’s Balanced Salt Solution. PC-3 (prostate; ATCC 30–2004) was kept in F-12 K medium supplemented with antibiotics, L-glutamine, sodium bicarbonate, FBS, HEPES,

(7) Development of TSSK1 and TSSK2 Targeted Degraders Reveals Sperm Machinery for Protein Degradation and Potential for Nonhormonal Male ContraceptionJournal of Medicinal ChemistryJune 25, 2026Jerrett A. Holdaway, Lokesh Saini, Raven Coil-Otto, Saman Nayyab, Mahboubeh Taghavi, Pablo Visconti, Gunda I. GeorgInvitrogen (catalog # PV3505). CHO-K1 cells and cell media (F12K) were obtained from ATCC (catalog # ATCC 30-2004). HiBiT-TSSK1 DNA plasmid (vector ID: VB231116-1266hdp) and piggyBac mRNA (catalog # R007S) were purchased

(8) Non-caveolar caveolin - 1 in retinal Müller glia promotes innate immune responsesJournal of Biological ChemistryJune 23, 2026Eric N. Enyong, Olawale O. Bankole, Jami M. Gurley, Mark E. McClellan, Libin Liu, Shi-Ying Ding, Martin-Paul Agbaga, Ana J. Chucair-Elliott, John D. Ash, Michael H. Elliott(1X) + GlutaMax-I (Invitrogen, Cat#: 10,566-016). PC3 cells were cultured in F-12K medium (ATCC Cat#: 30-2004). MIO-M1 Müller glia and PC3 culture media were supplemented with 10% (v/v) fetal bovine serum (FBS)

(9) MASQ: A multiplex qPCR platform for quantitative alternative splicing analysis in clinical specimensMol Ther Nucleic AcidsJune 16, 2026Hyejeong Jeong, Namjoon Cho, Jong-Il Park, Heon Jong Yoo, Kee K. Kim(Welgene). BeWo cells were cultured in F-12K medium (Kaighn’s modification of Ham’s F-12 medium; ATCC #30–2004) supplemented with 10% FBS and 1% penicillin/streptomycin. A549, K562, and Jurkat cells were cultured

(10) Developing a pan cancer therapy based on DISE inducing short RNAsMolecular Therapy Nucleic AcidsJune 16, 2026Andrea E. Murmann, Mehrnoosh Ebadi, Monal Patel, Alexander Ewe, Sergio Barajas, Shiyu Xiao, Min Jun Ko, Sanghee Lee, Wenjie Cai, Bidur Paudel, et al.(rat adrenal cancer) were cultured in F-12K medium (Kaighn’s modification of Ham’s F-12 medium) (ATCC-30-2004), supplemented with 2.5% FBS and 15% horse serum. ZFL cells (ATCC, CRL-2643, zebrafish, liver cancer)

(11) Combination of Selpercatinib and Trametinib Overcomes Resistance to RET Inhibitors in RET-Mutant Medullary Thyroid CarcinomaJCO Precis OncolJune 8, 2026David Milewski, Arwa Fallatah, Maya Groff, Abdelrahman Rahmy, Sophia R. Khan, Christian Okafor, Brittany Glassberg, Hsien-Chao Chou, Kerstin Heselmeyer-Haddad, Darawalee Wangsa, et al.the process of clonal selection. Cells were maintained in F12K media (ATCC, Manassas, VA, Cat. No. 30-2004) containing 10% fetal bovine serum and 1% antibiotic-antimycotic in a 5% CO2 incubator at 37°C. The

(12) Structural Diversity and Analytical Characterization of AcylhomocarnitinesJournal of Proteome ResearchJune 5, 2026Jaclyn Weinberg, William J. Crandall, Zachery Ryan Jarrell, Gahyun Lim, Ken Liu, Ho Young Lee, Shakshi Patel, Camilo Anthony Gacasan, Young-Mi Go, Dean P. Jones(41115712) were purchased from Waters (Milford, MA, USA). Human AC16 (CRL-3578) cells and ATCC F-12K medium (30–2004) were purchased from ATCC. BenchMark Fetal bovine serum (100–106) was purchased from GeminiBio (Sacramento,

(13) A novel Orthonairovirus detected in grey seal (Halichoerus grypus)One HealthJune 1, 2026Mikael Leijon, Louise Guevara, Gustav Averhed, Moa Naalisvaara Engman, Fereshteh Banihashem, Linnea Cervin, Britt-Marie Bäcklin, Neil E. Anderson, Siamak Zohari, Aleksija NeimanisCRL-1586, American Type Culture Collection, Manassas, VA, USA) and in ATCC-formulated F-12 K Medium (ATCC 30–2004) for inoculation onto A549 cells (ATCC Cat# CCL-185™), with both cell cultures grown in 25 cm2 flasks.

(14) Identification of the Annexin A2-interacting domain of pneumococcal PsaAmSphereMay 26, 2026Prattay Dey, Yoonsung Hu, Faith Henson, Chaeyoung Kim, Nogi Park, Keun Seok Seo, Justin A. Thorntonperformed using the human A549 epithelial cell line (ATCC CCL-185). Cells were grown in F12K medium (ATCC 30-2004) supplemented with 10% FBS and penicillin/streptomycin (Cytiva, Cat# SV30010) until 80%–90% confluency.

(15) Paired CRISPR screens identify mitochondrial metabolism and UBE2H as aneuploid-specific dependencies in human cancer cell linesbioRxivMay 4, 2026Klaske M. Schukken, Saron M. Akalu, Charles Zou, Pranav K. Kandikuppa, Ryan A. Hagenson, Jessica L. Keane, Mason P. Lynch, Toyoki Yoshimoto, Olaf Klingbeil, Erin L. Sausville, et al.media. HCT116 and RKO were cultured in McCoy-5A (ATCC, 30–2007) media. AGS were grown in F-12K (ATCC, 30–2004) media. MCF10A cells were grown in DMEM/F12 (Invitrogen, 11330–032) media with 5% Horse Serum (Gibco,

(16) OGDHL promotes prostate cancer progression and regulates neuroendocrine marker expression and nucleotide abundanceMol Cancer ResMay 4, 2026Matthew J. Bernard, Andrea Gallardo, Angel Ruiz, Johnny A. Diaz, Nicholas M. Nunley, Rachel N. Dove, Shile Zhang, Ernie Lee, Kylie Y. Heering, Grigor Varuzhanyan, et al.penicillin-streptomycin (Gibco: 15-140-122). PC-3 cells (RRID:CVCL_0035) were cultured in F-12K media (ATCC 30-2004) supplemented with 10% (v/v) FBS (Sigma-Aldrich: F0926), and 100 units/mL penicillin-streptomycin (Gibco:

(17) Network-level divergence in cyclic di-GMP signalling drives ecological versatility in Acinetobacter baumanniinpj Biofilms and MicrobiomesApril 9, 2026Rubén de Dios, Valerie S. Forsyth, Lyuboslava G. Harkova, Kylie J. Schache, Brian Y. Hsueh, Hannah Strat, Brynn Riley, Alejandro Rubio, Antonio J. Pérez-Pulido, Christopher M. Waters, et al.cells were cultured to confluence in 24-well plates in Kaighn’s Modification of Ham’s F-12 Medium (ATCC 30-2004) supplemented with 10% heat-inactivated foetal bovine serum at 37 °C and 5% CO2. Bacterial strains were

(18) The HosA histone deacetylase regulates stress resistance, host cell interactions, and virulence in Aspergillus fumigatusMicrobiology SpectrumApril 7, 2026Hong Liu, Pamela Lee, Alice Vo, Sanjoy Paul, Quynh T. Phan, Jianfeng Lin, Vincent M. Bruno, Mark A. Stamnes, W. Scott Moye-Rowley, Scott G. Fillerlines All human cell lines were purchased from the ATCC. A549 cells were cultured in F-12 K medium (ATCC, 30-2004) supplemented with 10% fetal bovine serum (Gemini Bio-Products) and 1% streptomycin and penicillin (Irvine

(19) Supramolecular coiled-coil peptide platform for site-specific antibody drug conjugate engineeringNature communicationsMarch 2, 2026Ringaci A, Shih TY, Grinstaff MW.maintain the cell lines: McCoy’s 5A Medium (ATCC 30-2007) for SKBR-3 and SKOV-3, F-12K Medium (ATCC 30-2004) for A549, and RPMI 1640 (ATCC 30-2001) for 4T1. The cell lines were cultured at 37 °C in a humidified

(20) A Time-Resolved RNA-Sequencing Dataset of Transcriptional Responses in PC12 cells to NGF Withdrawal and ReplenishmentData in BriefFebruary 26, 2026Peter Neufeld, Eliza Grlickova-Duzevik, Benjamin J. Harrisonmedia containing RPMI01640 media (ATCC, 30-2001) supplemented 10% Heat inactivated horse serum (ATCC, 30-2004) and 5% fetal bovine serum and supplemented with 1% penicillin/ streptomycin (Lonza BioWhittaker, 17-602E)

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8/4/2022Great F-12K Medium Luchas GutierrezI used this medium to grow, expand and treat my smooth muscle cells. I always got great results, and the price is really convenient. High quality and affordable.

F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) from ATCC

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ATCC

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F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) from ATCC

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