BG-GLA-NHS from New England Biolabs

Product Specs
ItemBG-GLA-NHS
CompanyNew England Biolabs
Price $451.00 Inquire
Catalog NumberS9151S
Quantity2 mg
ApplicationsLabeling fusion proteins
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Clathrin-independent endocytosis and retrograde transport in cancer cells tune immune synapse organization and CD8 T cell responseeLifeApril 22, 2026Shiqiang Xu, Alix Buridant, Thibault Hirsch, Céline Duhamel, Benjamin Ledoux, Massiullah Shafaq-Zadah, Estelle Dransart, Louise Thines, Ludger Johannes, Pierre Van Der Bruggen, et al.incubating them overnight at 4°C with a threefold molar excess of BG-GLA-NHS reagent (New England Biolabs, S9151S; prepared in anhydrous DMSO). The reaction was neutralized with 20 mM Tris (pH 7.4) for 10 min at 21°C.

(2) Stall force measurement of the kinesin-3 motor KIF1A using a programmable DNA origami nanospringeLifeMarch 25, 2026Nobumichi Takamatsu, Hiroko Furumoto, Takayuki Ariga, Mitsuhiro Iwaki, Kumiko Hayashi(Hokkaido System Science) (oligo 32A* and 32B* in Supplementary file 2) was conjugated with BG-GLA-NHS (S9151S; New England Biolabs) through the reaction between the ester and amine groups (BG-oligonucleotide).

(3) Protocol to reconstitute motor protein clustering on vesicle membranes using a DNA scaffold-based system in vitroSTAR ProtocMarch 20, 2026Rui Jiang, William O. Hancocktubulin,Uppalapati et al4,N/A NeutrAvidin,Thermo Fisher,Cat#: 31000 BG-GLA-NHS,New England Biolabs,Cat#: S9151S SYBR Green I nucleic acid gel stain,Lonza,Cat#: 50513 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine

(4) DNA nanodevice for analysis of force-activated protein extension and interactionsNature NanotechnologyDecember 15, 2025Kun Zhou, Minhwan Chung, Shankar Pandey, Jing Cheng, John T. Powell, Qi Yan, Jun Liu, Yong Xiong, Martin A. Schwartz, Chenxiang Linresuspended in Milli-Q ultrapure water at 2 mM concentration. The BG-GLA-NHS (New England Biolabs, S9151S) and HaloTag Succinimidyl Ester (O4) Ligand (PROMEGA, P6751) were reconstituted in DMSO or DMF to a

(5) A Practical Approach for Polarity and Quantity Controlled Assembly of Membrane Proteins into NanoliposomesChemBioChemMarch 15, 2025Shiwei Zhang, Peng Lin, Futa Komatsubara, Eiji Nakata, Takashi MoriiSection Materials: M13 single‐stranded viral DNA (p7308) was purchased from Guild Biosciences. BG‐GLA‐NHS (S9151S) and bovine serum albumin (BSA, BS9000S) were purchased from New England Biolabs. Purified oligonucleotides

(6) Enzyme Reactions Are Accelerated or Decelerated When the Enzymes Are Located Near the DNA NanostructureACS Appl Mater InterfacesMarch 12, 2025Peng Lin, Tomohiko Hayashi, Huyen Dinh, Eiji Nakata, Masahiro Kinoshita, Takashi MoriiMaterials The single-stranded M13mp18 viral DNA (7249) was purchased from Guild Biosciences. BG-GLA-NHS (S9151S) and Bovine Serum Albumin (BSA, BS9000S) were purchased from New England Biolabs. Purified DNA origami

(7) Artificial cells with all-aqueous droplet-in-droplet structures for spatially separated transcription and translationNat CommunJanuary 24, 2025Kanji Tomohara, Yoshihiro Minagawa, Hiroyuki NojiBG-Dex Benzyl-guanine conjugated Dextran was prepared from Amino Dextran 500k (Invitrogen, D7144) and BG-GLA-NHS (NEB, S9151S). 50 mM HEPES-KOH pH = 8.3, 1 wt% Amino Dextran, 0.16 mM BG-GLA-NHS freshly dissolved in DMSO (initial

(8) Quantifying protein unfolding kinetics with a high-throughput microfluidic platformbioRxivJanuary 18, 2025B. Atsavapranee, F. Sunden, D. Herschlag, P.M. Fordyce(benzyl guanine, BG) to BSA via N-hydroxysuccinimide (NHS) coupling, we dissolved NHS-GLA-BG (2 mg, S9151S, NEB) in 100 μL DMF, slowly added 20 μL of this solution to 1000 μL of 5 g/L buffer-exchanged BSA in

(9) DNA nanodevice for analysis of force-activated protein extension and interactionsbioRxivDecember 14, 2024Kun Zhou, Minhwan Chung, Jing Cheng, John T. Powell, Qi Yan, Jun Liu, Yong Xiong, Martin A. Schwartz, Chenxiang Linresuspended in Milli-Q ultrapure water at 2 mM concentration. The BG-GLA-NHS (New England Biolabs, S9151S) and HaloTag Succinimidyl Ester (O4) Ligand (PROMEGA, P6751) were reconstituted in DMSO or DMF to a

(10) A DNA-based molecular clamp for probing protein interactions and structure under forceACS NanoOctober 8, 2024Minhwan Chung, Kun Zhou, John T. Powell, Chenxiang Lin, Martin A. Schwartzuse. Conjugation of oligonucleotides to SNAP and Halo-tag Benzylguanine (BG, SNAP tag ligand BG-GLA-NHS, NEB, S9151S) and chloroalkane (CL, HaloTag Succinimidyl Ester (O4) Ligand, PROMEGA, P6751) were reconstituted in

(11) Cell type-specific delivery by modular envelope designNat CommunAugust 23, 2023Daniel Strebinger, Chris J. Frangieh, Mirco J. Friedrich, Guilhem Faure, Rhiannon K. Macrae, Feng Zhang0.5 mg/ml, before incubation with a 40-fold molar excess of BG-GLA-NHS (New England Biolabs, Cat. # S9151S). To test different ratios of BG-GLA-NHS, 10-, 20-, 40-, or 80-fold molar excess of BG-GLA-NHS was added

(12) Dissipation and energy propagation across scales in an active cytoskeletal materialProceedings of the National Academy of SciencesApril 4, 2023Peter J. Foster, Jinhye Bae, Bezia Lemma, Juanjuan Zheng, William Ireland, Pooja Chandrakar, Rémi Boros, Zvonimir Dogic, Daniel J. Needleman, Joost J. Vlassakdescribed by Chandrakar et al. (26). BG-labeled tubulin was made by labeling tubulin using BG-GLA-NHS (NEB S9151S) using a protocol by A. Hyman et al. (47). To create BG microtubules, 44 μM tubulin containing 6% BG-labeled

(13) CLASP2 recognizes tubulins exposed at the microtubule plus-end in a nucleotide state–sensitive mannerScience AdvancesJanuary 6, 2023Wangxi Luo, Vladimir Demidov, Qi Shen, Hugo Girão, Manas Chakraborty, Aleksandr Maiorov, Fazly I. Ataullakhanov, Chenxiang Lin, Helder Maiato, Ekaterina L. Grishchukof 5′-NH2-oligos at 2 mM, 8 μl of 200 mM Hepes buffer (pH 8.5), and 12 μl of BG-GLA-NHS (catalog no. S9151S, NEB, MA, USA) prepared in dimethyl sulfoxide at 20 mM. The mixture was incubated for 30 min at 25°C,

(14) The MIDAS domain of AAA mechanoenzyme Mdn1 forms catch bonds with two different substrateseLifeFebruary 11, 2022Keith J Mickolajczyk, Paul Dominic B Olinares, Brian T Chait, Shixin Liu, Tarun M Kapoor(BG) group was covalently attached to the amino-oligonucleotides using BG-GLA-NHS (New England BioLabs S9151S) under manufacturer-suggested reaction conditions, and subsequently purified using Micro Bio-Spin P-6

(15) Tight nanoscale clustering of Fcγ receptors using DNA origami promotes phagocytosiseLifeJune 3, 2021Nadja Kern, Rui Dong, Shawn M Douglas, Ronald D Vale, Meghan A MorrisseymM. N-hydroxysuccinimide ester (BG-GLA-NHS)-functionalized benzylguanine was purchased from NEB (Cat# S9151S) and freshly reconstituted in DMSO to a final concentration of 83 mM. To functionalize the oligonucleotides

(16) Improved resolution in single-molecule localization microscopy using QD-PAINTExperimental & Molecular MedicineMarch 1, 2021Yeonho Chang, Do-Hyeon Kim, Kai Zhou, Min Gyu Jeong, Soyeon Park, Yonghoon Kwon, Triet Minh Hong, Jungeun Noh, Sung Ho Ryudocking strands (2 mM) with NH2 in HEPES buffer (200 mM, pH 8.5) were reacted with BG-GLA-NHS (20 mM, #S9151S, New England Biolabs) in anhydrous dimethyl sulfoxide (DMSO, #D8418, Sigma-Aldrich) at RT for 30 min

(17) The Tail of Kinesin-14a in Giardia Is a Dual Regulator of MotilityCurrent BiologySeptember 1, 2020Kuo-Fu Tseng, Keith J. Mickolajczyk, Guangxi Feng, Qingzhou Feng, Ethiene S. Kwok, Jesse Howe, Elisar J. Barbar, Scott C. Dawson, William O. Hancock, Weihong QiuAC215092500 Gold nanoparticles 30nm,BBI Solutions,SKU# EM.GC30 BG-GLA-NHS,New England Biolabs,Cat# S9151S Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays Branson Ultrasonics™

(18) Enhanced enzymatic activity exerted by a packed assembly of a single type of enzyme†Chemical ScienceJuly 27, 2020Huyen Dinh, Eiji Nakata, Kaori Mutsuda-Zapater, Masayuki Saimura, Masahiro Kinoshita, Takashi Moriimethods Materials The single-stranded M13mp18, restriction enzymes (NdeI and NotI) and BG-GLA-NHS (S9151S) were purchased from New England Biolabs. Purified oligonucleotide as the staple strands for DNA origami,

(19) Unique features in the intracellular transport of typhoid toxin revealed by a genome-wide screenPLOS PathogensApril 5, 2019Shu-Jung Chang, Sheng Chih Jin, Xuyao Jiao, Jorge E. Galántyphoid toxin (50 μg) was incubated for 2 hr at room temperature with BG-NHS (New England BioLabs, Cat #S9151S) (20 mM stock solution in DMSO) at a molar ratio of 1:3. The unreacted esters were quenched with 50

(20) In vitro Generation of CRISPR-Cas9 Complexes with Covalently Bound Repair Templates for Genome Editing in Mammalian CellsBIO-PROTOCOLJanuary 5, 2019Nataša Savić, Femke Cas Ringnalda, Christian Berk, Katja Bargsten, Jonathan Hall, Martin Jinek, Gerald Schwankfunctional group (Integrated DNA Technologies)Benzylguanine-GLA-NHS (New England Biolabs, catalog number: S9151S)HEPES (Sigma-Aldrich, catalog number: H4034-500G)N, N-Dimethylformamide (DMF) (Sigma-Aldrich, catalog

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BG-GLA-NHS

BG-GLA-NHS from New England Biolabs

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New England Biolabs

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BG-GLA-NHS from New England Biolabs

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