HaloTag R110Direct Ligand from Promega

Product Specs
ItemHaloTag R110Direct Ligand
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG3221
Quantity30microl

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A high-throughput, flow cytometry approach to measure phase behavior and exchange in biomolecular condensatesNature CommunicationsJanuary 15, 2026Yuchen He, George M. Ongwae, Anupam Mondal, Joel A. Moses, Jeetain Mittal, Marcos M. PiresG8252), Coumarin-Chloroalkane (Coumarin-Cl, Promega, G8582), and R110-Chloroalkane (R110-Cl, Promega, G3221). These dyes were directly conjugated to the NPM1-Halo protein while it was bound to Ni-NTA resin, enabling

(2) Genetic evidence for PARP1 trapping as a driver of PARP inhibitor efficacy in BRCA mutant cancer cellsNucleic Acids ResDecember 11, 2025Jonathan Ribeiro, Kjetil Hansen, Lotte van Beek, Christopher Stubbs, Joan Frigola, Sara Talbot, Sabrina Bentouati, James Hall, Paul W G Wijnhoven, Josh Armenia, et al.media was changed. Then, cells were exposed to PARPi and to the HaloTag R110Direct ligand (Promega, #G3221, 1:1000 dilution). Cells were then incubated overnight at 37°C, 5% CO2. The next day, cells were exposed

(3) In vivo pulse-chase in Caenorhabditis elegans reveals intestinal histone turnover changes upon starvationJournal of Biological ChemistryJuly 1, 2025Christopher Borchers, Kara Osburn, Hyun Cheol Roh, Scott T. Aokibacteria and soaked for 20 min in the presence of 300 nM Halo-TMR (G8251, Promega), Halo-R110Direct (G3221, Promega, G3221), or Halo-Oregon Green (G2801 Promega). Worms incubated without HaloTag ligand served

(4) Lacritin cleavage-potentiated targeting of iron - respiratory reciprocity promotes bacterial deathJournal of Biological ChemistryMay 1, 2025Mohammad Sharifian Gh., Fatemeh Norouzi, Mirco Sorci, Tanweer S. Zaidi, Gerald B. Pier, Alecia Achimovich, George M. Ongwae, Binyong Liang, Margaret Ryan, Michael Lemke, et al.Na2HPO4, 0.18 mM KH2PO4, 0.27 mM KCl, 13.7 mM NaCl (pH 7.2), exposed to 50 μM of rhodamine chloroalkane (G3221, Promega) for 30 min at 37 °C with shaking, washed with 1 mM Na2HPO4, 0.18 mM KH2PO4, 0.27 mM KCl, 13.7

(5) Replication-dependent histone labeling dissects the physical properties of euchromatin/heterochromatin in living human cellsSci AdvMarch 28, 2025Katsuhiko Minami, Kako Nakazato, Satoru Ide, Kazunari Kaizu, Koichi Higashi, Sachiko Tamura, Atsushi Toyoda, Koichi Takahashi, Ken Kurokawa, Kazuhiro Maeshimalive-cell imaging. For Fig. 9, the parental H3.2-HaloTag was labeled with 10 nM HaloTag R110Direct ligands (G3221, Promega) overnight before the blocking with 7BRO. The following Repli-Histo labeling for single-nucleosome

(6) A role for the Cockayne Syndrome B (CSB)-Elongin ubiquitin ligase complex in signal-dependent RNA polymerase II transcriptionThe Journal of biological chemistryJuly 1, 2021Weems JC, Slaughter BD, Unruh JR, Weaver KJ, Miller BD, Delventhal KM, Conaway JW, Conaway RC.signal-dependent RNAPII transcription. Experimental procedures Materials HaloTag R110Direct Ligand G3221 and HaloTag TMRDirect Ligand G2991 were purchased from Promega. Hoechst solution (33,258; used at 1:1000

(7) Systematic quantitative analysis of ribosome inventory during nutrient stressNatureJuly 1, 2020An H, Ordureau A, Körner M, Paulo JA, Harper JW.1255–1), Biotin-PEG4-Alkyne (Click Chemistry Tools, TA105–25), HaloTag® R110Direct™ Ligand (Promega, G3221), HaloTag TMR (5mM) (Promega, G8251), 6-Mercaptopurine monohydrate (Sigma, 852678–1G-A), Sodium ascorbate

(8) SKP2 attenuates autophagy through Beclin1-ubiquitination and its inhibition reduces MERS-Coronavirus infectionNature communicationsDecember 18, 2019Gassen NC, Niemeyer D, Muth D, Corman VM, Martinelli S, Gassen A, Hafner K, Papies J, Mösbauer K, Zellner A, et al.according to manufacturer’s manual using R110Direct as halogenated fluorophore (100 nM, R110Direct, #G3221, Promega) over-night (pulse), followed by switching the cells to medium without dye (chase). Determination

(9) Discovery of novel inhibitors of ribosome biogenesis by innovative high throughput screening strategiesBiochemical JournalAugust 15, 2019Catherine E. Scull, Yinfeng Zhang, Nichole Tower, Lynn Rasmussen, Indira Padmalayam, Robert Hunter, Ling Zhai, Robert Bostwick, David A. SchneiderViCell and diluted to a final concentration of 400 000 cells/ml. R110 Direct ligand (Promega; cat# G3221) was added to the cells at 1 : 1000 final volume and 20 μl of the cell suspension was added to each

(10) Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19Journal of cell scienceJanuary 1, 0001"Majstrowicz K, Honnert U, Nikolaus P, Schwarz V, Oeding SJ, Hemkemeyer SA, Bähler M."1:100, P5182, Sigma-Aldrich), HaloTag TMR Ligand (5 µM, G8251, Promega), HaloTag R110Direct (5 µM, G3221, Promega), Mitotracker Green FM (50 nM, M7514, Thermo Fisher Scientific), Mitotracker Orange CMXRos

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