Cytek® Amnis® ImageStream®X Mk II Imaging Flow Cytometer from Cytek Biosciences

Product Specs
ItemCytek^® Amnis^® ImageStream^®^X Mk II Imaging Flow Cytometer
CompanyCytek Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumberImageStream
LasersUp to 6 laser options
Dimensions(WxDxH) 35 x 25 x 26 in. (889 x 635 x 660 mm)
QuantityEA
Objectives12 Images per Object: BrightField, SSC, Fluorescence 60x: 0.9 NA, 40 µm FOV, 0.3 µm/px,
40x: 0.75 NA, 60 µm FOV, 0.5 µm/px
20x: 0.5 NA, 120 µm FOV, 1 µm/px Pixel by pixel compensation
Excitation375 nm (70 mW), 405 nm, (120 mW) 488 nm (200/400 mW), 561 nm (200 mW), 592 nm (300 mW), 642 nm (150 mW)
Parameters12 fluorescence channels
CapacityMicroplates using autosampler (96-well)
Data Throughput2,000 to 5,000 cells/sec

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Cytek Biosciences

47215 Lakeview Blvd.

Fremont, CA 94538

United States

Phone: (877) 922-9835

Email: products@cytekbio.com

Company Profile

Website: https://cytekbio.com/

(1) Tracking Inter- and Intraspecific Bacterial Competition in Pairwise Assays Using a Transient Fluorescent DyeEcol EvolApril 7, 2026Julius Hoffmann, Ole Johannsen, Lutz Beckstaken and fixed with Formaldehyde (2% final concentration). These samples were analysed using the AMNIS ImageStream MkII (Cytek Biosciences B.V., Amsterdam, The Netherlands) imaging flow‐cytometer and their optical density

(2) Cell-SELEX identifies a DNA aptamer for highly selective in vivo siRNA delivery in cholangiocarcinomaJHEP ReportsMarch 16, 2026Brandon A. Wilbanks, Ayano Kabashima, Enis H. Ozmert, Jack W. Sample, Julia Driscoll, Danielle M. Carlson, Caroline D. Doherty, Brooke Kimball, Jayla Millender, Irene K. Yan, et al.ultracentrifuged again to remove excess aptamers. Aptamer-lactosome incorporation efficacy was evaluated on ImageStream X Mk II Image Flowcytometer (Cytek Biosciences, CA, USA). To observe cellular uptake, aptamer-decorated

(3) Bronchial epithelial cell-derived extracellular vesicle analysis using conventional, imaging, and nanoscale flow cytometry technologiesScientific ReportsFebruary 26, 2026Georgina Hopkins, William Browne, Davis Tucis, Stella Cochrane, Victoria James, David Onion, Lucy C. FaircloughBeckman Coulter), nano flow cytometer (CytoFLEX nano, Beckman Coulter), and imaging flow cytometer (ImageStream X MKII, Cytek Biosciences) were tested for measuring EVs. The instrument acquisition settings for each

(4) JUNO-coated beads as a functional assay to capture and characterize fertilization-competent human spermHuman Reproduction OpenFebruary 13, 2026Paula Cots-Rodríguez, Xinyin Wang, Mirian Sanchez-Tudela, Karen K Siu, Patrick Yip, Emilio Gómez, Jeffrey E Lee, Julieta G Hamze, Maria Jiménez-MovillaFluor 647 for 30 minutes in the dark. Fluorescence and brightfield images were acquired using an Amnis ImageStream Mark II imaging flow cytometer equipped with a high-gain camera at 60× magnification (Cytek Biosciences,

(5) A red blood cell–based antigen delivery system to facilitate T cell epitope presentation to promote peripheral tolerance to ADAMT S13 in immune-mediated thrombotic thrombocytopenic purpuraResearch and Practice in Thrombosis and HaemostasisFebruary 5, 2026Margot Dierickx, Stijn Groten, Mariarosaria Miranda, Pieter Langerhorst, Carmen van der Zwaan, Erik Mul, Arie J. Hoogendijk, Jan Voorbergbinding of TAT-CUB2 peptides to RBCs was monitored by flow cytometry and imaging flow cytometry (Cytek ImageStream Mark II). RBCs (5 × 106 per condition) were collected in HEPES-buffered saline supplemented with 0.5%

(6) CD8+ T-cell Antitumor Immunity via Human iNKT–DC ConjugatesCancer Immunology ResearchFebruary 3, 2026Dana C. Baiu, Kelsey A. Smith, Sarah A. Ferguson, Nathan R. Schwartz, Shashwot Tripathy, Joshua Brand, Huy Q. Dinh, Makoto Ohashi, Eric C. Johannsen, Jenny E. Gumperzusing FlowJo v9 and v10 software (BD Biosciences). Imaging flow cytometry was performed using an Amnis ImageStream MarkII (Cytek) imaging cytometer, and data processed using Ideas v6.2 (EMD Millipore) software. For

(7) Immune Niche Formation Reveals Mechanisms of Tumor Dormancy and Targeting OpportunitiesResearchSquareFebruary 2, 2026Abdul Yang, Abdul Ahad, Hiroshi Ichise, Hiroshi Ichise, Jae Young Schrom, Jae Young Sellner, Carter Sellner, Wenjuan Wang, Wenjuan Wang, Woo Yong Kieu, et al.dark conditions. The fluorescence intensities were measured with a 2-camera, 4-laser, 12-channel Amnis ImageStream MkII (Cytek Bioscience) imaging flow cytometer. The images were generated with INSPIRE software and

(8) Cellular Uptake of Nanoparticles is Regulated by Integrin-Based Adhesion to the Extracellular MatrixACS Applied Materials & InterfacesJanuary 21, 2026Sailesti Joshi, Ananya Naha, Justice Ene, Christina Holmes, Yan Li, Tristan Driscollincubating with 4% PFA for 20 min at room temperature. The nanoparticle uptake was then measured using an ImageStream®X MkII flow cytometry instrument and INSPIRE™ software (Cytek Biosciences, CA, USA) and analyzed against

(9) Targeting CDK12/13 Drives Mitotic Arrest to Overcome Resistance to KRASG12C InhibitorsCancer ResearchJanuary 16, 2026Yaakov E. Stern, Pompom Ghosh, John Peroza, Hitendra S. Solanki, Denis Imbody, Liznair Bridenstine, Thiyagamurthy Pandurangan, Sylvia M. Frydman, Kathryn N. Nickens, Hannah L. Walker-Mimms, et al.release into a thymidine-free medium for indicated time points. Samples were then acquired on an Amnis Imagestream® X MKII (ISXII) (Cytek Biosciences) (RRID:SCR_018589) with a dual CCD (charge-coupled device) camera

(10) Skin-derived myeloid precursors and joint-resident fibroblasts spread psoriatic disease from skin to jointsNat ImmunolJanuary 2, 2026Maria G. Raimondo, Hashem Mohammadian, Mario R. Angeli, Stefano Alivernini, Vladyslav Fedorchenko, Kaiyue Huang, Richard Demmler, Peter Rhein, Cong Xu, Yi-Nan Li, et al.80 mW, 5 mW and 150 mW, respectively. Image analysis was performed using image-based algorithms in ImageStream Data Exploration and Analysis Software (IDEAS 6.2.189, Cytek Biosciences). Typical files contained imagery

(11) Malignant Transformation of Human Uroepithelial Cells Induced by Long-Term Exposure to a Disinfection Byproduct─2,6-DichlorobenzoquinoneEnviron Health (Wash)December 19, 2025Tongtong Xiang, Chang Wang, Yasmine Rais, Andrei P. Drabovich, Xing-Fang Liusing an oil immersion lens. 2.8 Imaging Flow Cytometry (IFC) Imaging flow cytometry (IFC) (Amnis ImageStream mk II, Cytek Biosciences, Fremont, CA, USA) was performed on 80,000 cells per group to quantitatively

(12) C1q drives neural stem cell quiescence by regulating cell cycle and metabolism through BAI1Nat CommunDecember 11, 2025Katja M. Piltti, Anita Lakatos, Francisca Benavente-Perez, Zeina H. Elrachid, Aileen A. Nava, Nathaniel T. Addonizio, Victoria Nguyen, Atena Zahedi, Wei A. Song, Xiyu Chen, et al.HEK293T control lysate (Novus Biologicals; Supplementary Fig. 3a). Single cells were imaged using Amnis Imagestream x Mark II Imaging Flow Cytometer and analyzed using IDEAS version 6.2 (Cytek Biosciences)51,107 and

(13) From Dyes to Drugs? Selective Leishmanicidal Efficacy of Repositioned Methylene Blue and Its Derivatives in In Vitro EvaluationBiology (Basel)November 30, 2025Deyvison Rhuan Vasco-Dos-Santos, Juliana Almeida-Silva, Ludmila Ferreira de Almeida Fiuza, Natalia Vacani-Martins, Zênis Novais da da Rocha, Maria de Nazaré Correia Soeiro, Andrea Henriques-Pons, Eduardo Caio Torres-Santos, Marcos André Vannier-SantosAnnexin FITC/PI were analyzed by image flow cytometry. Sample acquisition was performed using the Amnis® ImageStream®X Mk II (Cytek® Biosciences Inc., Fremont, CA, USA) and analyzed with IDEAS software (version 6.3).

(14) Catalytic activity of FAHD1 correlates with nuclear morphology and proteomic states in human osteosarcoma cellsScientific ReportsNovember 25, 2025Andreas S. Pfarl, Anne Heberle, Alexander K. H. Weiss10% FCS, centrifuged at 300 × g for 3 min, and reseeded at a 1:5 dilution. Imaging flow cytometry (ImageStream) Nuclear size measurements were obtained using a Cytek® Amnis® ImageStream®X MkII imaging flow cytometer.

(15) Divergent stage-specific regulation of neutrophil function by glucose transporter 1 in murine antibody-mediated glomerulonephritisJCI InsightNovember 10, 2025Hossein Rahimi, Wonseok Choi, Doureradjou Peroumal, Shuxia Wang, Partha S. Biswasantibody (Abcam, clone EPR3915). The colocalization of Ly6G and Glut1 fluorescence was detected by Amnis ImageStream analysis (Cytek Biosciences) and expressed as a percentage of surface-expressed (Ly6G colocalized) Glut1

(16) Deguelin inhibits growth and prolactin synthesis in prolactinomas by targeting the PI3K/AKT/CREB3L1 pathway and ornithine decarboxylaseActa Pharmacologica SinicaNovember 3, 2025Lei Gong, Chang-xiao-feng Liu, Jian-hua Cheng, Jing Guo, Bin Li, Hong-yun Wang, Meng Liu, Jia-lin Wang, Xue-jing Li, Qiu-yue Fang, et al.FITC Annexin V Apoptosis Detection Kit I (556547, BD Biosciences, San Jose, CA, USA). The results were analyzed by flow cytometry (Amnis® ImageStream®X MK II, Cytek, Fremont, CA, USA), and the results were processed using IDEAS application v6.2 software. Prediction

(17) Single-atom Pt-doped ceria nanozymes mitigate myocardial ischemia reperfusion injury via cardiomyocyte-targeted uptake and suppression of reactive oxygen speciesBioactive materialsNovember 1, 2025Pu A, Sim WS, Ji Y, Kurian AG, Lee JH, Van Anh Bui T, Lai Y, Hwangbo H, Sun H, Kim HW, et al.from each group were resuspended with 100 μl PBS and analyzed using an Imaging flowcytometer (Amnis ImageStream X MKII) (Cytek Biosciences, USA). At least 2000 cells were included for each analysis using Amnis IDEAS software

(18) Cross-Species Morphology Learning Enables Nucleic Acid-Independent Detection of Live Mutant Blood CellsbioRxivOctober 24, 2025Sarmad Ahmad Khan, Dominik Faerber, Danielle Kirkey, Simon Raffel, Brandon Hadland, Michael Deininger, Florian Buettner, Helong Gary Zhaocommittees. Single-cell imaging and ML Single-cell images were obtained in a high-throughput fashion using Amnis ImageStream® imaging flow cytometer (Cytek Biosciences, Fremont, CA). Before imaging, 10 μL of PB sample was transferred

(19) Assessing Extracellular Vesicle Turnover In Vivo Using Highly Sensitive Phosphatidylserine-Binding ReagentsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2025Flaskamp L, Prechtl M, Scheck A, Hu W, Ried C, Kislinger G, Simons M, Krug AB, Kranich J, Brocker T.to constitute a cluster. Imaging Flow Cytometry for EV Analysis The imaging flow‐cytometer (IFC) ImageStream MKII (Cytek) requires small volumes of EV sample (25–200 µL). It was opted to use a staining volume

(20) Imaging Flow Cytometry as a Molecular Biology Tool: From Cell Morphology to Molecular MechanismsInternational Journal of Molecular SciencesSeptember 23, 2025Yoshikazu Matsuoka[39,40,41,42,43,44,45,46,47,48]. 2.2. Current Applications of IFC Currently, commercially available IFC systems are typified by the ImageStream®X Mark II and FlowSight® (both from Cytek Biosciences, formerly Amnis). The ImageStream®X Mark II can

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Cytek^® Amnis^® ImageStream^®^X Mk II Imaging Flow Cytometer

Cytek^® Amnis^® ImageStream^®^X Mk II Imaging Flow Cytometer from Cytek Biosciences

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Cytek^® Amnis^® ImageStream^®^X Mk II Imaging Flow Cytometer from Cytek Biosciences

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