Cytek® Guava® easyCyte™ Flow Cytometer from Cytek Biosciences

Product Specs
ItemCytek^® Guava^® easyCyte^™ Flow Cytometer
CompanyCytek Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumbereasyCyte
Throughput1,000 – 10,000 cells/test
DetectionFSC, SSC, 12 fluorescent colors depending on model
ApplicationsCancer Research, Stem Cell Research, Signaling, QC and Maintenance,
Chemokine Analysis, Cell Viability
QuantityEA
Number of ChannelsUp to 14
Lasers1 to 3 lasers
Excitation488 nm (50/150 mW), 532 nm (100 mW), 642 nm (100 mW)
Parameters14 parameters / 12 fluorescence channels
CapacityTubes (1.5 mL, 2 mL), Microplates (96-well)
Data Throughput1,000 events/sec
Dimensions(WxDxH) 54 x 52 x 52 cm
Flow Rate7 to 70 µL/min

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Cytek Biosciences

47215 Lakeview Blvd.

Fremont, CA 94538

United States

Phone: (877) 922-9835

Email: products@cytekbio.com

Company Profile

Website: https://cytekbio.com/

(1) Ursodeoxycholic Acid Exhibits Greater Inhibitory Effects on Cancerous HCT116 Colon Cells than on Noncancerous NCM460 Colon CellsNutrientsMarch 19, 2025Huawei Zeng, Bryan D. Safratowich, Wen-Hsing Cheng, Mary Briske-Andersonevaluation, each sample was analyzed with at least 5000 and 2000 single-cell events, respectively, using a Guava EasyCyte 6 HT flow cytometry analyzer (Cytek Biosciences, Fremont, CA, USA). 2.3. Western Blotting Analysis Briefly,

(2) A Potent, Selective, Small-Molecule Inhibitor of DHX9 Abrogates Proliferation of Microsatellite Instable Cancers with Deficient Mismatch RepairCancer researchFebruary 17, 2025Castro J, Daniels MH, Brennan D, Johnston B, Gotur D, Lee YT, Knockenhauer KE, Lu C, Wu J, Nayak S, et al.Staining proceeded according to Guava Cell Cycle Reagent protocol. The cell cycle was determined using a Guava EasyCyte flow cytometer (Cytek Biosciences) with Guava Cell Cycle software module (RRID: SCR_025377). Analysis

(3) Cryptic genetic variation shapes the fate of gene duplicates in a protein interaction networkNat CommunFebruary 11, 2025Soham Dibyachintan, Alexandre K. Dubé, David Bradley, Pascale Lemieux, Ugo Dionne, Christian R. Landry10x in sterile water in a 96 well plate (Greiner). For each clone, 5000 events were acquired with a Guava EasyCyte instrument (Cytek Bio). We calculated the log2 median fluorescence intensity for each clone from the

(4) Comparison of immune responses to respiratory syncytial virus in infancy, childhood, and adulthood using an in vitro model of human respiratory infectionImmunoHorizonsJanuary 24, 2025Christiana Smith, Kaili Curtis, Adrianne Bonham, Shea Boyer, Laurel Lenz, Adriana Weinbergmedia containing gentamicin (Lonza; cat. 04-418Q). Cell counts and viability were obtained using a Guava easyCyte 5HT System (Cytek Biosciences) instrument and samples with a minimum of 80% viability and 3,000,000

(5) Maternal Injectable Mineral Administration Effects on Calf Growth and Reproductive ParametersAnimals (Basel)January 24, 2025Makayla A. Brenner, Rodrigo S. Marques, Christian J. Posbergh, Abigail L. Zezeski, Thomas W. Geary, Sarah R. McCoskibNCM to a final concentration of 40 × 106 per mL for analysis. Samples were analyzed via FC using a Guava EasyCyte B/G/V-HT equipped with violet (405 nm, 110 mW), blue (488 m, 160 mW), and green (532 nm, 110 mW) lasers

(6) pH-Responsive Polyethylene Glycol Engagers for Enhanced Brain Delivery of PEGylated Nanomedicine to Treat GlioblastomaACS NanoJanuary 14, 2025Jun-Lun Meng, Zi-Xuan Dong, Yan-Ru Chen, Meng-Hsuan Lin, Yu-Ching Liu, Steve R. Roffler, Wen-Wei Lin, Chin-Yuan Chang, Shey-Cherng Tzou, Tian-Lu Cheng, et al.mmol L–1 NaCl, pH 5.8) three times and the surface fluorescence of 104 viable cells was measured by Guava easyCyte Flow Cytometer (Cytek Biosciences) and analyzed with Flowjo (Tree Star Inc.). Fluorescence Microscopy

(7) TRPM7 activity drives human CD4 T-cell activation and differentiation in a magnesium dependent mannerbioRxivDecember 9, 2024Kilian Hoelting, Anna Madlmayr, Birgit Hoeger, Dorothea Lewitz, Marius Weng, Tanja Haider, Michelle Duggan, Rylee Ross, F. David Horgen, Markus Sperandio, et al.RPMI or RPMI with 6 mM MgCl2 for 5 days. Proliferation was analyzed daily using Guava ViaCount on a Guava Easycyte 12HT flow cytometer (Cytek Bioscoences, Fermont, TX, USA). Proliferation experiments on primary T cells

(8) The riboflavin biosynthetic pathway as a novel target for antifungal drugs against Candida speciesmBioNovember 13, 2024Jana Nysten, Arne Peetermans, Dries Vaneynde, Stef Jacobs, Liesbeth Demuyser, Patrick Van Dijckgreen fluorescence (GRN B-Hlog) that corresponds to the riboflavin wavelengths, was measured using the Guava easyCyte Flow Cytometer (Cytek). In all, 10,000 cells were excited with a 488 nm laser, and the emitted light

(9) Production of Oncolytic Measles Virus in Vero Cells: Impact of Culture Medium and Multiplicity of InfectionVirusesNovember 6, 2024Dustin Eckhardt, Jana Mueller, Jonas Friedrich, Jan-P. Klee, Irakli Sardlishvili, Lars E. Walter, Stefanie Fey, Peter Czermak, Denise Salzigtransferred 200 μL of the cell suspension to a 96-well plate with a flat bottom for an analysis using a Guava easyCyte flow cytometer (Cytek Biosciences, Fremont, CA, USA) and guavaSoft Incyte software (version 4.5). The

(10) Long-Life Inoculant: Bradyrhizobium Stored in Biodegradable Beads for Four Years Shows Optimal Cell Vitality, Interacts with Peanut Roots, and Promotes Early GrowthPlants (Basel)October 25, 2024Adriana Belén Cesari, Marilina Fernandez, Natalia Soledad Paulucci, Marta Susana Dardanellisolution for subsequent analysis by flow cytometry. Flow cytometric (FCM) measurements were performed on a Guava® easyCyte system (Cytek® Biosciences, Fremont, CA, USA), with excitation at 488 nm. The optical filters were set

(11) Selective Inhibition of Deamidated Triosephosphate Isomerase by Disulfiram, Curcumin, and Sodium Dichloroacetate: Synergistic Therapeutic Strategies for T-Cell Acute Lymphoblastic Leukemia in Jurkat CellsBiomoleculesOctober 13, 2024Luis A. Flores-López, Ignacio De la Mora-De la Mora, Claudia M. Malagón-Reyes, Itzhel García-Torres, Yoalli Martínez-Pérez, Gabriela López-Herrera, Gloria Hernández-Alcántara, Gloria León-Avila, Gabriel López-Velázquez, Alberto Olaya-Vargas, et al.compensation matrix and subsequent analysis of experimental samples. Cell analysis was carried out using a Guava® easyCyte™ Flow Cytometer (Cytek® Biosciences, Fremont, CA, USA), with data acquisition and analysis performed

(12) The fitness cost of spurious phosphorylationThe EMBO JournalSeptember 10, 2024David Bradley, Alexander Hogrebe, Rohan Dandage, Alexandre K Dubé, Mario Leutert, Ugo Dionne, Alexis Chang, Judit Villén, Christian R Landrytemperature in the dark. Finally, 5000 events were recorded for each condition with a flow cytometer (Guava EasyCyte instrument, Cytek Bio). Flow cytometry competition assay For this experiment, kinase and kinase-dead

(13) Adiponectin pathway activation dampens inflammation and enhances alveolar macrophage fungal killing via LC3-associated phagocytosisbioRxiv : the preprint server for biologyAugust 24, 2024Goli SH, Lim JY, Basaran-Akgul N, Templeton SP.incubated with the IgG secondary antibodies [33]. Analyses were performed by flow cytometric analysis on a Guava EasyCyte HT system and Cytek. Flow cytometric data were analyzed using FlowJo software. BALF ELISA analysis Serum

(14) Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly†Biology of ReproductionJune 12, 2024Peter Sutovsky, Lauren E Hamilton, Michal Zigo, Mayra E Ortiz D’Avila Assumpção, Alexis Jones, Filip Tirpak, Yuksel Agca, Karl Kerns, Miriam Sutovskywide range of biomedical applications, including human and animal andrology. An early example is the EasyCyte flow cytometer adapted as Cytek Guava instrument for sperm-specific tasks by AI supplier IMV Technologies

(15) Clinical validation of C12FDG as a marker associated with senescence and osteoarthritic phenotypesAging CellMay 6, 2024William S. Hambright, Victoria R. Duke, Adam D. Goff, Alex W. Goff, Lucas T. Minas, Heidi Kloser, Xueqin Gao, Charles Huard, Ping Guo, Aiping Lu, et al.on Cytek® Northern Lights (16v, 14b) cytometer. The single‐color TCs and PBMCs assay were run on a Guava® easyCyte™ HT (Guava®) flow cytometer in triplicate. The instrument settings for the Cytek Cytometer were set

(16) Cryptic genetic variation shapes the fate of gene duplicates in a protein interaction networkbioRxivMarch 1, 2024Soham Dibyachintan, Alexandre K Dube, David Bradley, Pascale Lemieux, Ugo Dionne, Christian R Landry10x in sterile water in a 96 well plate (Greiner). For each clone, 5000 events were acquired with a Guava EasyCyte instrument (Cytek Bio). We calculated the median fluorescence intensity for each clone from the

(17) Efficacy of Butyrate to Inhibit Colonic Cancer Cell Growth Is Cell Type-Specific and Apoptosis-DependentNutrientsFebruary 14, 2024Sema Oncel, Bryan D. Safratowich, James E. Lindlauf, Zhenhua Liu, Daniel G. Palmer, Mary Briske-Anderson, Huawei Zengsuspended in growth media. At least 2000 cell events for each sample were examined for apoptosis by the Guava EasyCyte 6HT flow cytometry analyzer with GuavaSoft 3.3 (Cytek Biosciences, Fremont, CA, USA). 2.3. Western Blotting

(18) Implication of IZUMO2 in the cell‐in‐cell phenomenon: A potential therapeutic target for triple‐negative breast cancerThoracic CancerJanuary 23, 2024Toshiya Higashi, Chiemi Saigo, Wakana Chikaishi, Hirokatsu Hayashi, Yuki Hanamatsu, Manabu Futamura, Nobuhisa Matsuhashi, Tamotsu Takeuchigoat anti‐mouse IgG (H + L) (cat. no. A11001; Invitrogen) for 30 min at 4°C. The cells were analyzed using a Guava EasyCyte cell analyzer (Cytek Biosciences). The occurrence of the cell‐in‐cell phenomenon was evaluated using

(19) Activin A induces apoptosis of human lung adenocarcinoma A549 cells through endoplasmic reticulum stress pathwayOncology ReportsDecember 20, 2023Fenglin Zhang, Yan Qi, Jing Li, Boyang Liu, Zhonghui Liu, Xueling Cuimark the cells for 15 min in the dark. Finally, the labeled cells were analyzed using flow cytometry (guava easyCyte HT; Cytek Biosciences). FlowJo (FlowJo_v10.6.2; FlowJo LLC) was used to collect and analyze the data

(20) A flow-cytometry-based pipeline for the rapid quantification of C2C12 cell differentiationSTAR protocolsDecember 15, 2023Parisi B, Sünnen M, Chippalkatti R, Abankwa DK.study,https://doi.org/10.5281/zenodo.8325331 ,, Other,Other,Other ,, CO2 incubator,Panasonic,Cat# MCO-170AICUVL-PA Guava easyCyte 6HT 2L flow cytometer,Cytek Biosciences,N/A Microcentrifuge Micro Star 17R,VWR,Cat# 521-1647 Z1 particle

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