FluoroFix™ Buffer from BioLegend

Product Specs
ItemFluoroFix™ Buffer
CompanyBioLegend
Price $153.00Supplier Page View Company Product Page
Catalog Number422101
ApplicationsFC, ICFC - Quality tested, ICC - Reported in the literature, not verified in house
Quantity200 tests

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) SPNS2 exports sphingosine-1-phosphate and imports glucoseNat CommunApril 20, 2026Cynthia Weigel, Md Lokman Hossen, Ryan D. R. Brown, Can E. Senkal, Christopher D. Green, Jason Newton, Sumit Saha, Elisa N. D. Palladino, Bin Ni, Francesco S. Celi, et al.Alomone Labs) for 30 min at room temperature. After washing, cells were then fixed with FluoroFix Buffer (#422101, BioLegend). Cells were washed and resuspended in flow buffer for analysis with a BD LSRFortessa X-20 Cell Analyzer

(2) A fully human pan VL9 HLA-E TCRm antibody enables functional dissection of HLA-E biology and checkpoint signalingiScienceApril 9, 2026Soroush Ghaffari, Katherine Upchurch-Ange, Gizem Oter, Trivendra Tripathi, Susanne Gimlin, Debra Wawro Weidanz, Jim Middelburg, Thorbald van Hall, Jon A. Weidanzbeads",Agilent,Cat#K007811-8 DPBS,Gibco,Cat#14040133 FBS,Cytiva,Cat#SH30406 FluoroFix buffer,Biolegend,Cat#422101 gentleMACS Octo Dissociator,Miltenyi Biotec,Cat#130-134-029 Ghost viability_V450,Tonbo biosciences,Cat#13-0863 Ghost

(3) Effects of oncolytic immunotherapy with RP1 (vusolimogene oderparepvec) on immune cells mediate responsiveness to anti-PD-1 via STING-mediated interferon signalingJ Immunother CancerMarch 3, 2026Victoria Roulstone, Joan Kyula, Elizabeth Appleton, Praveen K Bommareddy, Anton Patrikeev, Sylwia Jones, Linta Kuncheria, Charleen Ml Chan Wah Hak, Shane Foo, Holly Baldock, et al.Cells were then washed in FACS buffer and fixed in 100 µL of fluorofix fixation buffer (BioLegend, 422101) and incubated at 4°C for 30 min. Samples were resuspended in 1 mL of PBS and filtered prior to reading

(4) Aroclor 1254 inhibits anti-inflammatory macrophage polarization through an AhR-dependent mechanismJournal of the Endocrine SocietyJanuary 7, 2026Riley M Behan-Bush, Elizabeth Kilburg, Jesse N Liszewski, Michael V Schrodt, Edward A Sander, Aloysius J Klingelhutz, James A Ankrumtemperature incubation, cells were washed in PBS and fixed with FluoroFix Buffer (BioLegend, Cat#: 422101) for 30 minutes. Permeabilization was carried out using Intracellular Staining Permeabilization Wash

(5) Epidermal Interferon‐κ Drives Cutaneous Lupus‐Like Lesions, Photosensitivity, and Systemic Autoimmunity In VivoArthritis & RheumatologyJanuary 1, 2026Benjamin Klein, Deborah J. Colesa, Yiqing Gao, Patrick A. O'Brien, Lin Zhang, Lori Lowe, Kelsey E. McNeely, Nguyen Thi Kim Nguyen, Svenja Henning, Mehrnaz Gharaee‐Kermani, et al.were washed with phosphate buffered saline (PBS), fixed with Fluorofix Buffer solution (BioLegend, 422101), and resuspended in PBS. Samples were stored at 4°C and measured using a Cytek Aurora Spectral Analyzer.

(6) Aging-induced hepatocyte CD44 drives IL6/STAT3 signaling and associates with impaired neighboring T cell functionbioRxivDecember 26, 2025Armin Gandhi, Kathryn Lande, Yichen Li, Filipe A. Hoffman, Michael LaPorte, Shirong Tan, Garrett Evensen, Benji Portillo, Marcos Garcia Teneche, Rouven Arnold, et al.stained with a fixable viability dye (eBioscience #65–0865-14) and fixed (BioLegend FluoroFix Buffer #422101). The hepatocytes were blocked with anti-CD16/CD32 (1:250, TruStain FcX™ PLUS anti-mouse CD16/32, BioLegend

(7) Sez6L2 autoimmunity induces cerebellar ataxia in miceJournal of NeuroinflammationNovember 25, 2025Carlos J. Reyes-Sepúlveda, Julia M. Granato, John Randolph, Allison T. Ryan, Alison Hobbins, Angela Stout, Nina Silver, Herman Li, Jennetta W. HammondCells were then washed three times with FACS buffer and incubated in FluoroFix™ buffer (BioLegend, 422101) for 60 min. The cells were resuspended in FACS buffer and incubated at 4 °C overnight. The following

(8) Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugsCell Rep MethodsNovember 14, 2025Krieg K, Materna-Reichelt S, Naber T, Rachad FZ, Kauven P, Weller A, Haferkamp U, Wittich A, Zaliani A, Woo MS, et al.FACSymphony A3. To confirm GFP expression, cells were fixed in FluoroFix Fixation Buffer (BioLegend, Cat# 422101) for 20 min and analyzed using Gallios 10C 3L Flow Cytometer (Beckmann Coulter). For phenotyping, cells

(9) CpG ODN Activates TLR9 and Upregulates TLR3 via the p38 MAPK-ATF3 Signaling Axis to Synergistically Enhance Dendritic Cell Vaccine EfficacyCellsNovember 13, 2025Lv Zhou, Zhuowei Lei, Qian Jiang, Linpeng Xu, Quanji Wang, Yimin Huang, Ting Leirequiring intracellular staining, after the aforementioned steps, cells were fixed using FluoroFix™ Buffer (422101, BioLegend, San Diego, CA, USA). Fixed cells were washed with diluted 10× Intracellular Staining Permeabilization

(10) Gut-primed neutrophils activate Kupffer cells to promote hepatic injury in mouse sepsisNature CommunicationsNovember 10, 2025Atsushi Murao, Alok Jha, Takayuki Kato, Junji Shimizu, Yuichi Akama, Monowar Aziz, Ping WangCD11b Ab, and APC anti-F4/80 Ab (clone: BM8; BioLegend), and then fixed in Fluorofix buffer (Cat. No.: 422101; BioLegend), followed by intracellular staining with PE anti-iNOS Ab (clone: C-11; Santa Cruz Biotechnology,

(11) Systematic discovery of CRISPR-boosted CAR T cell immunotherapiesNatureSeptember 24, 2025Paul Datlinger, Eugenia V. Pankevich, Cosmas D. Arnold, Nicole Pranckevicius, Jenny Lin, Daria Romanovskaia, Moritz Schaefer, Francesco Piras, Anne-Christine Orts, Amelie Nemc, et al.a cell strainer. For storage before cell sorting, cells were fixed in FluoroFix Buffer (BioLegend, 422101) at room temperature for 30 min in the dark, followed by washing cells twice and storing them in Cell Staining Buffer

(12) Targeting Kupffer Cell Enolase 1 Attenuates Liver Inflammation and Injury in Hemorrhagic ShockInternational Journal of Molecular SciencesAugust 28, 2025Zhijian Hu, Jingsong Li, Naureen Rashid, Asha Jacob, Ping WangAPC anti-mouse F4/80 antibody (Cat. No. 123116, BioLegend). The cells were then fixed using Fluorofix buffer (Cat. No. 422101; BioLegend, San Diego, CA, USA), followed by intracellular staining for Enolase 1 (Cat. No. MA5-32756;

(13) Oral Bioavailability of a Noncoding RNA Drug, TY1, That Acts on MacrophagesJ Extracell BiolAugust 14, 2025Shukuro Yamaguchi, Kazutaka Miyamoto, Xaviar M. Jones, Alessandra Ciullo, Kara Tsi, Jessica Anderson, Hiroaki Komuro, Salwa Soussi, Ashley Morris, Diana Kitka, et al.centrifugation at 800 × g for 5 min. Finally, cells were resuspended in 500 µL of FluoroFix Buffer (422101, BioLegend). Unstained and isotype control samples were also prepared. 3.17 Statistical Analysis Statistical

(14) Inducible HIV-1 Reservoir Reduction Assay (HIVRRA), a Fast and Sensitive Assay to Test Cytotoxicity and Potency of Cure Strategies to Reduce the Replication-Competent HIV-1 Reservoir in Ex Vivo PBMCsBIO-PROTOCOLJuly 20, 2025Jade Jansen, Teunis B. H. Geijtenbeek, Neeltje A. KootstraViolet (ThermoFisher Scientific, catalog number: C34557) 14. Fluorofix (BioLegend, catalog number: 422101) 15. Glycerol (Sigma-Aldrich, catalog number: 818709) 16. Triton X-100 (Fisher Scientific, catalog number:

(15) Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemiaNature Biomedical EngineeringJuly 1, 2025Chao Ma, Huishu Wang, Lunan Liu, Ruiqi Chen, Nandana Mukherjee, Jie Tong, Shadab Kazmi, Xiangyi Fang, Matthew T. Witkowski, Iannis Aifantis, et al.then thoroughly washed 3 times with PBS followed by fixation using FluoroFix Buffer (catalogue number 422101, BioLegend) for 20 min. To stain Ki67 (1:50, APC anti-human Ki67, catalogue number 350514, or Alexa

(16) Sez6L2 autoimmunity induces cerebellar ataxia in micebioRxivMay 31, 2025Carlos J. Reyes-Sepúlveda, Julia Granato, John Randolph, Allison T. Ryan, Alison Hobbins, Angela Stout, Herman Li, Jennetta W. HammondCells were then washed three times with FACS buffer and incubated in FluoroFix™ buffer (BioLegend, 422101) for 60 minutes. The cells were resuspended in FACS buffer and incubated at 4°C overnight. The following

(17) Palbociclib and dsRNA sensor co-operate to enhance anti-cancer effects through ER stress and modulation of immune evasionNat CommunMay 25, 2025Victoria Roulstone, Joan Kyula-Currie, James Wright, Emmanuel C. Patin, Isaac Dean, Lu Yu, Aida Barreiro-Alonso, Miriam Melake, Jyoti Choudhary, Richard Elliott, et al.(65-0865-14, eBioscience) for 30 mins. Cells were washed in PBS + 5% FBS and fixed in FluoroFix buffer (422101, BioLegend) prior to analysis on the BD LSR II analyser or the BD Symphony A5. Data was collected using

(18) DNA methylation predicts infection risk in kidney transplant recipientsLife Sci AllianceMay 5, 2025Fei-Man Hsu, Harry Pickering, Liudmilla Rubbi, Michael Thompson, Elaine F Reed, Matteo Pellegrini, Joanna M Schaenmanand stained with fluorochrome-conjugated monoclonal antibodies, then fixed in FluoroFix buffer (Cat# 422101; BioLegend) using standard procedures as previously described (Schaenman et al, 2022). Antibodies used

(19) Polychlorinated biphenyls induce immunometabolic switch of anti-inflammatory macrophages toward an inflammatory phenotypePNAS NexusMarch 27, 2025Riley M Behan-Bush, Michael V Schrodt, Elizabeth Kilburg, Jesse N Liszewski, Laura M Bitterlich, Karen English, Aloysius J Klingelhutz, James A Ankrumtemperature, the macrophages were washed with PBS and fixed using FluoroFix Buffer (BioLegend, cat#: 422101). After 30 min of fixation, the cells were permeabilized using Intracellular Staining Permeabilization

(20) Identification of a conserved subset of cold tumors responsive to immune checkpoint blockadeJ Immunother CancerMarch 6, 2025Jade Moore, Jim Gkantalis, Ines Guix, William Chou, Kobe Yuen, Ann A Lazar, Matthew Spitzer, Alexis Combes, Mary Helen Barcellos-HoffThe supernatant was aspirated, and the cell pellet was in 0.5 mL/tube FluoroFix buffer (BioLegend Cat#422101) in the dark for 1 hour at room temperature for fixation. For intracellular staining, the nucleus was

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FluoroFix™ Buffer from BioLegend

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FluoroFix™ Buffer from BioLegend

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