Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Pharmacological Inhibition of Small Extracellular Vesicle Secretion by ALK5i SD-208 via Lysosomal Rerouting of CD63+ CompartmentsJ Extracell VesiclesAugust 29, 2026Rahul Sanwlani, Georgina H. Thompson, Kyle Bramich, Ching-Seng Ang, Millie L. Trowbridge, Chris E. Duringer, Vlad Stolojan, Sean M. Davidson, Konstantinos Savvatis, John H. McVey, et al.Cell Staining Buffer. For intracellular staining, samples were fixed in 500 µL Fixation Buffer (BioLegend, 420801) for 20 min, then centrifuged at 400 × g for 5 min. These samples were subsequently washed and permeabilised

(2) H3.3 chaperone Hira primes the effector program and function of regulatory T cellsSci AdvAugust 21, 2026Xiangxiang Cao, Mengjie Lv, Zhihan Lv, Shuting Zheng, Xinyi Tan, Jiyu Ding, Wei Liang, Guohong Li, Xuyu Zhou, Mingzhao Zhu1640 for 20 min at 37°C in a 5% CO2 incubator. Cells were subsequently fixed with a Fixation Buffer (420801, Biolegend) and permeabilized with a True-Phos Perm Buffer (425401, Biolegend) according to the manufacturer’s

(3) Dissociation method influences immune cell composition in porcine tonsil cell suspensionsFront ImmunolAugust 13, 2026Luise Hohensee, Alexa Eisenmann, Emma L. Lemberger, Xin Qu, Tanja Groll, Katja Steiger, Friederike EbnerFACS buffer at a staining volume of 30µL per sample. For intracellular staining, the fixation buffer (420801, Biolegend, USA) was used according to manufacturer’s instructions, intranuclear fixation was achieved

(4) AFF3 maintains metabolic quiescence in naïve CD8 T cells and prevents premature immune agingJCI InsightAugust 10, 2026Molly E. Lumnitzer, Stefanie F. Valbon, Stephanie A. Condotta, Allison E. Norlander, Sheng Liu, Jun Wan, Martin J. Richerat 4°C for 20 minutes, followed by another wash. Cells were treated with fixation buffer (BioLegend, 420801) and incubated at 4°C for 10 minutes, followed by a wash. Cells were resuspended in FACS buffer and

(5) Human bronchial air-liquid interface responses to nebulised protease exposureFront ImmunolAugust 7, 2026Georgina Hopkins, David Sheffield, Hugh Barlow, Anja Lalljie, Victoria James, Stella Cochrane, Lucy C. FaircloughPBS; 200 µL apically and 750 µL basally. Cells were then fixed with 4% paraformaldehyde (v/v) (Cat no. 420801, Biolegend), 200 µL applied apically and 750 µL basally, for 30 minutes at room temperature. The fixation

(6) Localized immunomodulation with cytokine-producing cells to mitigate foreign body responses in rodents and a nonhuman primateSci AdvAugust 7, 2026Boram Kim, Dilrasbonu Vohidova, Amanda Nash, Yuen San Chan, Samantha Fleury, Shravani Deo, Danna Murungi, Peter D. Rios, Ira Joshi, Hafsa Nasir, et al.with cell staining buffer and fixed for an hour at room temperature with fixation buffer (catalog no. 420801, BioLegend). After fixation, cells in each sample were resuspended in cell staining buffer and run through

(7) Cblb Gene Editing in T Cells Sustains Expansion and Immunogenic CAR T Tumor Killing Under Chronic Antigenic StimulationCellsAugust 3, 2026Daniel Schreiber, Sebastian Peer, Christina Lutz-Nicoladoni, Jiří Koutník, Viktor Lang, Viana Wille, Isabel Hölzl, Dominik Humer, Nino Tokic, Dorothee Freimark, et al.AB_2561389). After staining, cells were washed with buffer C and fixed in a Fixation Buffer (BioLegend, 420801) for 20 min at RT. Subsequently, cells were washed twice with 1× Intercellular Staining Permeabilization

(8) Modeling stimulus-induced stress responses in microglia-like cells using a commercial iPSC-dCas9-KRAB lineStem Cell ResearchAugust 1, 2026Devin M. Saunders, Nicola A. Kearns, Bernard Ng, Faraz Sultan, Himanshu Vyas, Ricardo A. Vialle, Eric M. Clark, Sashini De De Tissera, Jishu Xu, David A. Bennett, et al.min, then washed twice with FACS buffer. Stained cells were fixed with Fixation Buffer (Biolegend, 420801) according to the manufacturer’s instructions. If applicable, fixed cells were permeabilized using Intracellular

(9) ST6GAL1 Glycoengineering Rewires Cytokine Signaling and Preserves Metabolic Fitness in CAR-T Cells Under Galectin-3-Mediated ImmunosuppressionInt J Mol SciJuly 18, 2026Lee Seng Lau, Maria Suarez, Aizada Berdalinova, Rebecca Z. Fan, Rajib Kumar Shil, Joseph Souchak, Kim Tieu, Avery D. Posey, Charles J. DimitroffFollowing stimulation, cells were fixed via the addition of pre-warmed Fixation Buffer (BioLegend; Cat. No. 420801, San Diego, CA, USA) and incubated for 15 min at 37 °C to preserve the phosphorylation status. Cells

(10) Naive CD4+ T cells synthesize a hyaluronan-rich glycocalyx that is diminished following activationiScienceJuly 17, 2026Laxmi Prasad Adhikari, Jessica Contreras, Kristen M. Patterson, Meghan M. Moran, Erin N. Howe, Anna H.K. Plaas, Joseph M. ReynoldsEasySep Mouse CD4+ T cell Isolation Kit,STEMCELL Technologies,Cat#: 19852 Fixation Buffer,Biolegend,Cat#: 420801 M-MLV Reverse Transcriptase,Thermo Fisher Scientific,Cat#: 28025013 ,, Deposited data,Deposited data,Deposited

(11) Dendritic cell redundancy enables priming of anti-tumor CD4+ T cells in pancreatic cancerCancer CellJuly 13, 2026Courtney T.S. Kureshi, Michael J. Walsh, Rakeeb Kureshi, Victoire Cardot-Ruffino, Dennis A. Agardy, Lestat R. Ali, James M. Dougan, Li Qiang, Jiao Shen, Chong Zuo, et al.for 5 min. After discarding supernatants, cells were incubated in 200 μL Fixation Buffer (BioLegend #420801) for 20 min at room temperature. Cells were centrifuged at 700 g for 5 min. Supernatants were discarded

(12) Senescence-associated loss of intestinal α1,2-fucose disrupts a modifiable host-microbiome homeostasis axis in people with HIVbioRxivJuly 10, 2026Leila B. Giron, Maliha W. Shaikh, Thaisa M Cantu Cantu Jungles, Lijuan Zhang, Phillip A. Engen, Nuseybe Bulut, Shalini Singh, Jenna M. Hasson, Ellen Zhang, Shivanjali Shankaran, et al.Cells were then washed to remove unbound antibody and fixed with paraformaldehyde (BioLegend, catalog# 420801). Data were acquired on a FACSymphony A5 Spectral Analyzer. Measurement of plasma markers of microbial

(13) Protocol to purify human and mouse cytokines using an endotoxin-free Escherichia coli-based platformSTAR ProtocJuly 9, 2026Jae-Yoon Kim, Jessica Xin, I-Chih Kuo, Si Xuan Chen, Ahmed Kabil, Kai-Wei Chang, Nika Shakiba, Kelly M. McNagny, Yanpu HeMouse Recombinant IL-33,BioLegend,580506 Recombinant Human IL-33 (carrier-free),BioLegend,581804 Fixation buffer,BioLegend,420801 Recombinant mouse LIF,R&D Systems,8878-LF MEK inhibitor (PD0325901),Tocris Bioscience,4192/10 Embryonic stem cell

(14) Pink1-mediated mitophagy in the endothelium releases proteins encoded by mitochondrial DNA and activates neutrophil responses during inflammationElifeJuly 1, 2026Priyanka Gajwani, Li Wang, Koushik Debnath, Pierina Danos, Young-Mee Kim, Shubhi Srivastava, Zijing Ye, Sarah Krantz, Dong-Mei Wang, Chinnaswamy Tiruppathi, et al.with gentle shaking. Cells were fixed by adding an equal volume of Fixation buffer (Biolegend Cat# 420801) for 10 min, washed, and then analyzed by flow cytometry. For endothelial ICAM-1 expression, the median

(15) Hepatic Arachidonic Acid Dysregulation Impairs Double-Negative Regulatory T Cell-Mediated Immunoregulation via CD39 Downregulation and Drives MASLD DevelopmentJ Immunol ResJune 25, 2026Hua Jin, Xiyu Wang, Xinjuan Liu, Dong Zhang, Guangyong Sun(53‐6.7), and anti‐CD39 (Duha59). For intracellular staining, the cells were fixed with fixation buffer (420801, BioLegend) and subsequently stained with either anti‐granzyme B (QA16A02) or anti‐TNF‐α (MP6‐XT22).

(16) Precise control of switchable chimeric antigen receptor T cells allows enhanced safety and less T cell exhaustionJournal of Translational MedicineJune 17, 2026Zihan Anna Zhang, Logan Herring, Thuzar Hla Shwe, Yue Hu, Xiaotong Song, Wenyue Cao, Wenshe Ray Liustained with CD3 antibody, then washed with FACS buffer and fixed with fixation Buffer (Biolegend, 420801) for 20 min. After washing, cells were permeabilized and labelled with intracellular staining permeabilization

(17) Intermediate accumulated upon interruption of fatty acid oxidation flux promotes tumoral ferroptosis and improves immunotherapyNature CommunicationsJune 17, 2026Yuhan Zhou, Jing Li, Fangfang Liu, Yuan Gao, Songlin Yin, Liguo Yang, Xiaoxiao Li, Junhong Lin, Yan Li, Haotian Shang, et al.Biolegend) for 30 min at room temperature. Cells were washed, fixed and permeabilized by Fixation Buffer (420801, Biolegend) and Perm/Wash Buffer (421002, Biolegend), respectively. Then, cells were stained with anti-IFNγ

(18) Case Report: Immune checkpoint inhibitor-associated myocarditis, myositis, and myasthenia gravis overlap syndrome with flow cytometric phenotyping before and after treatment in a patient with urothelial carcinomaFrontiers in ImmunologyJune 15, 2026Karen Gambina, Carly Tymm, Matthieu Paiola, Kurenai Tanji, John Zech, Robert Winchester, Adam Mor, Yevgeniya GartshteynWaltham, Massachusetts, USA). Cells were then fixed with 4% paraformaldehyde Fixation Buffer (BioLegend #420801) and treated with Intracellular Staining Fixation Buffer (BioLegend 420801 and 421002) before intracellular

(19) The Effects of CB2R Activation on Inflammatory Pathways in DermatomyositisBiomedicinesJune 7, 2026Rohan Dhiman, Ahmed Eldaboush, Navin Vijayarangan, Darae Kang, Nilesh Kodali, DeAnna Diaz, Caroline Stone, Rui Feng, Victoria P. Werth30 min, after which the cells were washed with PBS and fixed with fixation buffer (Biolegend USA, #420801). This was then followed by resuspension in permeabilization buffer (Biolegend USA, #421002). The following

(20) LLMs uncover 6M1@C60’s therapeutic targets: inhibiting atherosclerotic progression via NRF2/HO-1/GPX4 axis-mediated ferroptosis suppressionJournal of NanobiotechnologyJune 2, 2026Wenhao Liu, Guofeng Zhou, Xin Gao, Bo Li, Yingjie Zhaothrough a 70-µm strainer. After red blood cell lysis (C3702, Beyotime; 5 min), cells were fixed (0.5 mL; 420801, BioLegend) for 20 min at room temperature, washed in 1× PBS containing 2% BSA, and permeabilized using

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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