FOXP3 Fix/Perm Buffer (4X) from BioLegend

Product Specs
ItemFOXP3 Fix/Perm Buffer (4X)
CompanyBioLegend
Price $71.00Supplier Page View Company Product Page
Catalog Number421401
ApplicationsICFC - Quality tested
Quantity30 mL
TargetFOXP3

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Targeting DAP5 Disrupts Alternate Mode of Translational Initiation in Tregs and Potentiates Antitumor ImmunityAdvanced ScienceFebruary 1, 2026Xiaojiang Lai, Guihong Pan, Xixi Feng, Jun Zhang, Yunxia Li, Shanmeizi Zhao, Zhenming Lin, Yun Feng, Wei Bao, Hongyan Yang, et al.staining, cells were washed twice with staining buffer and fixed with fixation buffer (Biolegend, Cat#421401) for 15 min at RT. For intracellular staining, fixed cells were permeabilized using Perm buffer (BD

(2) Liver-specific paraoxonase-1 alleviates regulatory T cell-driven immunosuppression via metabolic reprogramming in hepatocellular carcinomaNat CommunDecember 12, 2025Zhou Lu, Huanchen Shi, Rongshan Gao, Chengjie Zhong, Wei Zhang, Jian Zhu, Jianhang Huang, Ronghua Liu, Yun Xing, Yiwei Chu, et al.washed twice. Intracellular molecular staining was performed using the FOXP3 Fix/Perm Buffer (Biolegend, 421401) according to the manufacturer’s protocol. For cytokine detection, cells were stimulated in vitro with

(3) WNT16 from decidual stromal cells orchestrates M2 macrophage polarization via β-catenin signaling and chromatin remodeling at the maternal-fetal interfaceFront ImmunolDecember 8, 2025Lingyu Chang, Jiani Guo, Xi Xue, Yang Yan, Xinyi Li, Weijie Zhao, Xiaoli Sun, Jiajia Chen, Meirong Duincubated with cell surface antibodies for 30min at room temperature. Added 1mL FOXP3 Fix/Perm buffer (421401, BioLegend, USA) into each sample for fixation and permeabilization at room temperature for 20min, the

(4) Inhibition of bruton’s tyrosine kinase directly alleviates pathogenic CD4+ T cell response via PPAR-γ signaling in autoimmune uveitisJournal of NeuroinflammationNovember 27, 2025Zhiqiang Xiao, Zhuang Li, Wenjie Zhu, Guanyu Chen, Xiaoshuang Luo, Zhiqing Mai, Minzhen Wang, Haixiang Huang, Zuoyi Li, Yuxun Shi, et al.and surface markers as described above, and fixed/permeabilized using the FOXP3 Fix/Prem Buffer (CAT#421401, Biolegend, USA) and Permeabilization Buffer (CAT#00–8333-56, Invitrogen, USA). Intracellular antibodies

(5) Mannose metabolism reshapes T cell differentiation to enhance anti-tumor immunityCancer CellJanuary 13, 2025Yajing Qiu, Yapeng Su, Ermei Xie, Hongcheng Cheng, Jing Du, Yue Xu, Xiaoli Pan, Zhe Wang, Daniel G. Chen, Hong Zhu, et al.480043 Annexin V Binding Buffer,BioLegend,Cat# 422201 FOXP3 Fix/Perm Buffer (10X),BioLegend,Cat# 421401 Nuclear protein and cytoplasmic protein extraction kit,Proteintech,Cat# PK10014 Seahorse XF Cell Mito Stress Test Kit

(6) Cytotoxic T cells drive doxorubicin-induced cardiac fibrosis and systolic dysfunctionNature Cardiovascular ResearchJuly 17, 2024Abraham L. Bayer, Maria A. Zambrano, Sasha Smolgovsky, Zachary L. Robbe, Abul Ariza, Kuljeet Kaur, Machlan Sawden, Anne Avery, Cheryl London, Aarti Asnani, et al.factors, samples were surface stained as described, fixed using 1× FOXP3 fix/perm solution (Biolegend, 421401) for 20 min at RT, permeabilized for 15 min using 1× FOXP3 perm solution (Biolegend, 421402), then stained

(7) Macro CD5L(+) deteriorates CD8(+)T cells exhaustion and impairs combination of Gemcitabine-Oxaliplatin-Lenvatinib-anti-PD1 therapy in intrahepatic cholangiocarcinomaNature communicationsJanuary 20, 2024Lu JC, Wu LL, Sun YN, Huang XY, Gao C, Guo XJ, Zeng HY, Qu XD, Chen Y, Wu D, et al.g, 6 min, 4°C). For intracellular staining, added 1 mL of 1× FOXP3 Fix/Perm solution (Biolegend, Cat.421401, without dilution) to each tube; vortexed and incubated at room temperature in the dark for 20 min,

(8) Suppression of Tumor Cell Lactate-generating Signaling Pathways Eradicates Murine PTEN/p53-deficient Aggressive-variant Prostate Cancer via Macrophage PhagocytosisClinical Cancer ResearchDecember 1, 2023Kiranj Chaudagar, Hanna M. Hieromnimon, Anne Kelley, Brian Labadie, Jordan Shafran, Srikrishnan Rameshbabu, Catherine Drovetsky, Kaela Bynoe, Ani Solanki, Erica Markiewicz, et al.staining, single cell suspensions were washed with PBS and incubated with fix/perm buffer (Biolegend; 421401) for 15 minutes. After fixation, single cell suspensions of samples incubated with myeloid antibody

(9) Suppression of Tumor Cell Lactate-generating Signaling Pathways Eradicates Murine PTEN/p53-deficient Aggressive-variant Prostate Cancer via Macrophage PhagocytosisClinical cancer research : an official journal of the American Association for Cancer ResearchDecember 1, 2023Chaudagar K, Hieromnimon HM, Kelley A, Labadie B, Shafran J, Rameshbabu S, Drovetsky C, Bynoe K, Solanki A, Markiewicz E, et al.staining, single cell suspensions were washed with PBS and incubated with fix/perm buffer (Biolegend; 421401) for 15 minutes. After fixation, single cell suspensions of samples incubated with myeloid antibody

(10) Euphorbia factor L1 suppresses breast cancer liver metastasis via DDR1-mediated immune infiltrationAging (Albany NY)September 15, 2023Dongjing Jiang, XiaoQin Gao, RuLan Tan, Xun Liu, Ye Zhu, Li Zhangantibodies for staining according to the surface antigen staining protocol above, Foxp3 Fix/Perm Buffer (4×, 421401, Biolegend) was added for fixing and breaking membrane. Cells were then incubated with Foxp3 antibody

(11) Reversal of Lactate and PD-1-mediated Macrophage Immunosuppression Controls Growth of PTEN/p53-deficient Prostate CancerClinical cancer research : an official journal of the American Association for Cancer ResearchMay 15, 2023Chaudagar K, Hieromnimon HM, Khurana R, Labadie B, Hirz T, Mei S, Hasan R, Shafran J, Kelley A, Apostolov E, et al.stained single cell suspensions were washed with PBS and incubated with fix/perm buffer (Biolegend; 421401) for 15 minutes. Following fixation, single cell suspensions of samples incubated with myeloid antibody

(12) Galectin-3 expression in donor T cells reduces GvHD severity and lethality after allogeneic hematopoietic cell transplantationCell reportsMarch 28, 2023Mohammadpour H, Tsuji T, MacDonald CR, Sarow JL, Rosenheck H, Daneshmandi S, Choi JE, Qiu J, Matsuzaki J, Witkiewicz AK, et al.assays “The Big Easy” EasySep Magnet,StemCell Technologies,Cat# 18001 FOXP3 Fix/Perm Kit,BioLegend™,Cat# 421401 CellTrace™ Violet Cell Proliferation Kit,Invitrogen™,Cat# C34557 LIVE/DEAD™ Fixable Aqua Dead Cell Stain

(13) Targeting interleukin 4 receptor alpha on tumor-associated macrophages reduces the pro-tumor macrophage phenotypeNeoplasia (New York, N.Y.)October 1, 2022de Groot AE, Myers KV, Krueger TEG, Brennen WN, Amend SR, Pienta KJ.temperature, and stored overnight in Flow Buffer at 4°C. Samples were incubated in 1x FoxP3 Fix/Perm Solution (421401, BioLegend) in the dark for 20 minutes at room temperature and washed with 1x FoxP3 Perm Buffer (421402,

(14) Secreted osteopontin from CD4⁺ T cells limits acute graft-versus-host diseaseCell reportsDecember 28, 2021Aggarwal N, Deerhake ME, DiPalma D, Shahi SK, Gaggioli MR, Mangalam AK, Shinohara ML.assays,Critical commercial assays Cytofix/Cytoperm Kit,BD,Cat# 554714 FOXP3 Fix/Perm Kit,BioLegend,Cat# 421401 CellTrace Violet Cell Proliferation Kit,Invitrogen,Cat# C34557 LIVE/DEAD Fixable Violet Dead Cell Stain

(15) Characterization of tumor-associated macrophages in prostate cancer transgenic mouse modelsThe ProstateJuly 1, 2021de Groot AE, Myers KV, Krueger TEG, Kiemen AL, Nagy NH, Brame A, Torres VE, Zhang Z, Trabzonlu L, Brennen WN, et al.temperature, and stored overnight in FACS buffer at 4°C. Samples were incubated in 1x FoxP3 Fix/Perm Solution (421401, BioLegend) in the dark for 20 minutes at room temperature and washed with 1x FoxP3 Perm Buffer (421402,

(16) Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin MImmunityMarch 9, 2021Deerhake ME, Danzaki K, Inoue M, Cardakli ED, Nonaka T, Aggarwal N, Barclay WE, Ji RR, Shinohara ML.Assays,Critical Commercial Assays Cytofix/Cytoperm Kit,BD,Cat# 554714 FOXP3 Fix/Perm Kit,BioLegend,Cat# 421401 CellTrace Violet Cell Proliferation Kit,Invitrogen,Cat# C34557 LIVE/DEAD Fixable Violet Dead Cell Stain

(17) Th17/Treg-cell balance in the peripheral blood of pregnant females with a history of recurrent spontaneous abortion receiving progesterone or cyclosporine AExperimental and therapeutic medicineJanuary 1, 2021Wang S, Li M, Sun F, Chen C, Ye J, Li D, Qian J, Du M.used. For intracellular staining, cells were fixed and permeabilized using the Fix/Perm kit (cat. no. 421401/2). The fix/perm kit and all antibodies aforementioned were purchased from BioLegend, Inc. Flow cytometry

(18) T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal InflammationCell reportsJune 30, 2020Yan J, Pandey SP, Barnes BJ, Turner JR, Abraham C.,BD Biosciences,Cat# 558049 Perm Buffer III,BD Biosciences,Cat# 558050 FOXP3 Fix/Perm Buffer (4x),BioLegend,Cat# 421401 FOXP3 Perm Buffer (10x),BioLegend,Cat# 421402 Hexadecyltrimethylammonium bromide,MilliporeSigma,Cat#

(19) Multi-parametric flow cytometry staining procedure for analyzing tumor-infiltrating immune cells following oncolytic herpes simplex virus immunotherapy in intracranial glioblastomaJournal of biological methodsJanuary 1, 2019Bommareddy PK, Lowe DB, Kaufman HL, Rabkin SD, Saha D.)FoxP3 fix/perm buffer set (BioLegend, Cat. # 421403) contains 30 ml 4× concentrated FoxP3 fix/perm buffer (Cat. # 421401) and 25 ml 10× concentrated FoxP3 perm buffer (Cat. # 421402)HSV-1 glycoprotein B (gB) HSV-1-specific

(20) Transgelin induces dysfunction of fetal endothelial colony-forming cells from gestational diabetic pregnanciesAmerican journal of physiology. Cell physiologyOctober 1, 2018Varberg KM, Garretson RO, Blue EK, Chu C, Gohn CR, Tu W, Haneline LS.incubation. For mitosis experiments, cells were collected by trypsinization, fixed in 1⫻ Fix/Perm Buffer (421401; Biolegend), washed in Perm/Wash buffer (554723; BD Biosciences), stained with an Alexa 488 Fluorconjugated

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FOXP3 Fix/Perm Buffer (4X) from BioLegend

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FOXP3 Fix/Perm Buffer (4X) from BioLegend

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