FOXP3 Fix/Perm Buffer (4X) from BioLegend

Product Specs
ItemFOXP3 Fix/Perm Buffer (4X)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number421401
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
Quantity30 mL
TargetFOXP3
TypeSOLUTION

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Euphorbia factor L1 suppresses breast cancer liver metastasis via DDR1-mediated immune infiltrationAging (Albany NY)September 15, 2023Dongjing Jiang, XiaoQin Gao, RuLan Tan, Xun Liu, Ye Zhu, Li Zhangantibodies for staining according to the surface antigen staining protocol above, Foxp3 Fix/Perm Buffer (4×, 421401, Biolegend) was added for fixing and breaking membrane. Cells were then incubated with Foxp3 antibody

(2) Reversal of Lactate and PD-1–mediated Macrophage Immunosuppression Controls Growth of PTEN/p53-deficient Prostate CancerClinical Cancer ResearchMay 15, 2023Kiranj Chaudagar, Hanna M. Hieromnimon, Rimpi Khurana, Brian Labadie, Taghreed Hirz, Shenglin Mei, Raisa Hasan, Jordan Shafran, Anne Kelley, Eva Apostolov, et al.stained single cell suspensions were washed with PBS and incubated with fix/perm buffer (Biolegend; 421401) for 15 minutes. Following fixation, single cell suspensions of samples incubated with myeloid antibody

(3) Galectin-3 expression in donor T cells reduces GvHD severity and lethality after allogeneic hematopoietic cell transplantationCell ReportsMarch 28, 2023Hemn Mohammadpour, Takemasa Tsuji, Cameron R. MacDonald, Joseph L. Sarow, Hanna Rosenheck, Saeed Daneshmandi, Jee Eun Choi, Jingxin Qiu, Junko Matsuzaki, Agnieszka K. Witkiewicz, et al.assays “The Big Easy” EasySep Magnet,StemCell Technologies,Cat# 18001 FOXP3 Fix/Perm Kit,BioLegend™,Cat# 421401 CellTrace™ Violet Cell Proliferation Kit,Invitrogen™,Cat# C34557 LIVE/DEAD™ Fixable Aqua Dead Cell Stain

(4) Targeting interleukin 4 receptor alpha on tumor-associated macrophages reduces the pro-tumor macrophage phenotypeNeoplasiaOctober 1, 2022Amber E. de Groot, Kayla V. Myers, Timothy E.G. Krueger, W. Nathaniel Brennen, Sarah R. Amend, Kenneth J. Pientatemperature, and stored overnight in Flow Buffer at 4°C. Samples were incubated in 1x FoxP3 Fix/Perm Solution (421401, BioLegend) in the dark for 20 minutes at room temperature and washed with 1x FoxP3 Perm Buffer (421402,

(5) Secreted osteopontin from CD4⁺ T cells limits acute graft-versus-host diseaseCell reportsDecember 28, 2021Aggarwal N, Deerhake ME, DiPalma D, Shahi SK, Gaggioli MR, Mangalam AK, Shinohara ML.assays,Critical commercial assays Cytofix/Cytoperm Kit,BD,Cat# 554714 FOXP3 Fix/Perm Kit,BioLegend,Cat# 421401 CellTrace Violet Cell Proliferation Kit,Invitrogen,Cat# C34557 LIVE/DEAD Fixable Violet Dead Cell Stain

(6) Characterization of tumor‐associated macrophages in prostate cancer transgenic mouse modelsThe ProstateJuly 1, 2021Amber E. de Groot et al.temperature, and stored overnight in FACS buffer at 4°C. Samples were incubated in 1x FoxP3 Fix/Perm Solution (421401, BioLegend) in the dark for 20 minutes at room temperature and washed with 1x FoxP3 Perm Buffer (421402,

(7) Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin MImmunityMarch 9, 2021Deerhake ME, Danzaki K, Inoue M, Cardakli ED, Nonaka T, Aggarwal N, Barclay WE, Ji RR, Shinohara ML.Assays,Critical Commercial Assays Cytofix/Cytoperm Kit,BD,Cat# 554714 FOXP3 Fix/Perm Kit,BioLegend,Cat# 421401 CellTrace Violet Cell Proliferation Kit,Invitrogen,Cat# C34557 LIVE/DEAD Fixable Violet Dead Cell Stain

(8) Th17/Treg-cell balance in the peripheral blood of pregnant females with a history of recurrent spontaneous abortion receiving progesterone or cyclosporine AExperimental and therapeutic medicineJanuary 1, 2021Wang S, Li M, Sun F, Chen C, Ye J, Li D, Qian J, Du M.used. For intracellular staining, cells were fixed and permeabilized using the Fix/Perm kit (cat. no. 421401/2). The fix/perm kit and all antibodies aforementioned were purchased from BioLegend, Inc. Flow cytometry

(9) T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal InflammationCell reportsJune 30, 2020Yan J, Pandey SP, Barnes BJ, Turner JR, Abraham C.,BD Biosciences,Cat# 558049 Perm Buffer III,BD Biosciences,Cat# 558050 FOXP3 Fix/Perm Buffer (4x),BioLegend,Cat# 421401 FOXP3 Perm Buffer (10x),BioLegend,Cat# 421402 Hexadecyltrimethylammonium bromide,MilliporeSigma,Cat#

(10) Multi-parametric flow cytometry staining procedure for analyzing tumor-infiltrating immune cells following oncolytic herpes simplex virus immunotherapy in intracranial glioblastomaJournal of biological methodsJanuary 1, 2019Bommareddy PK, Lowe DB, Kaufman HL, Rabkin SD, Saha D.FoxP3 fix/perm buffer set (BioLegend, Cat. # 421403) contains 30 ml 4× concentrated FoxP3 fix/perm buffer (Cat. # 421401) and 25 ml 10× concentrated FoxP3 perm buffer (Cat. # 421402) HSV-1 glycoprotein B (gB) HSV-1-specific

(11) Transgelin induces dysfunction of fetal endothelial colony-forming cells from gestational diabetic pregnanciesAmerican journal of physiology. Cell physiologyOctober 1, 2018Varberg KM, Garretson RO, Blue EK, Chu C, Gohn CR, Tu W, Haneline LS.incubation. For mitosis experiments, cells were collected by trypsinization, fixed in 1⫻ Fix/Perm Buffer (421401; Biolegend), washed in Perm/Wash buffer (554723; BD Biosciences), stained with an Alexa 488 Fluorconjugated

(12) Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1Molecular cellAugust 16, 2018Cha JH, Yang WH, Xia W, Wei Y, Chan LC, Lim SO, Li CW, Kim T, Chang SS, Lee HH, et al.CD8-APC/Cy7 (100713, 1:200; BioLegend), and CD45-APC (103111, 1:100; BioLegend) antibodies. After fixation (421401, BioLegend) and permeabilization (421402, BioLegend), intracellular INFγ was stained using INFγ-Pacific

(13) 1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosisCell death & diseaseDecember 1, 2016Wei C, Mei J, Tang L, Liu Y, Li D, Li M, Zhu X.

(14) Yeast-Derived Particulate β-Glucan Treatment Subverts the Suppression of Myeloid-Derived Suppressor Cells (MDSC) by Inducing Polymorphonuclear MDSC Apoptosis and Monocytic MDSC Differentiation to APC in CancerJournal of immunology (Baltimore, Md. : 1950)March 1, 2016Albeituni SH, Ding C, Liu M, Hu X, Luo F, Kloecker G, Bousamra M 2nd, Zhang HG, Yan J.

(15) Selective ORAI1 Inhibition Ameliorates Autoimmune Central Nervous System Inflammation by Suppressing Effector but Not Regulatory T Cell FunctionJournal of immunology (Baltimore, Md. : 1950)January 15, 2016Kaufmann U, Shaw PJ, Kozhaya L, Subramanian R, Gaida K, Unutmaz D, McBride HJ, Feske S.

(16) Critical Role for the Adenosine Pathway in Controlling Simian Immunodeficiency Virus-Related Immune Activation and Inflammation in Gut Mucosal TissuesJournal of virologySeptember 1, 2015He T, Brocca-Cofano E, Gillespie DG, Xu C, Stock JL, Ma D, Policicchio BB, Raehtz KD, Rinaldo CR, Apetrei C, et al.

(17) Iraq Dust Is Respirable, Sharp, and Metal-Laden and Induces Lung Inflammation With Fibrosis in Mice via IL-2 Upregulation and Depletion of Regulatory T CellsJournal of Occupational & Environmental MedicineMay 5, 2014Anthony M. Szema, Richard J. Reeder, Andrea D. Harrington, Millicent Schmidt, Jingxuan Liu, Marc Golightly, Todd Rueb, Sayyed A. Hamidiper 5 µl antibody for 20 minutes) followed by fixing with FoxP3 fix/perm buffer (Biolegend Cat. No. 421401) at room temperature for 20 minutes. The cells were then permeabilized in FoxP3 perm buffer (Biolegend

(18) VIP Regulates the Development & Proliferation of Treg in vivo in spleenAllergy, Asthma & Clinical ImmunologyDecember 1, 2011Anthony M Szema, Sayyed A Hamidi, Marc G Golightly, Todd P Rueb, John J Chensuspension l antibody for 20 minutes followed by fixing with FoxP3 fix/perm buffer (Biolegend Cat. No. 421401) at room temperature for 20 minutes. The cells were then permeabilized in FoxP3 perm buffer (Biolegend

(19) Colour coding in the superior temporal sulcus of rhesus monkey visual cortexProceedings of the Royal Society of London. Series B, Containing papers of a Biological character. Royal Society (Great Britain)May 4, 1977S M Zeki

Be the First to Write a Review!

FOXP3 Fix/Perm Buffer (4X) from BioLegend

Description

BioLegend

Citations (19)

Reviews

FOXP3 Fix/Perm Buffer (4X) from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map