Fibroblast Basal Medium from ATCC

Product Specs
ItemFibroblast Basal Medium
CompanyATCC
Price $50.00Supplier Page View Company Product Page
Catalog NumberPCS-201-030
FormatpH: 7.4 ± 0.2; Osmolality: 270 ± 10 mOsm; Endotoxin: <0.5 EU/mL.
ApplicationsFibroblast Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support fibroblasts derived from normal human epidermis. Fibroblast Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of fibroblasts (e.g., Dermal Fibroblasts, Normal, Human Neonatal [ATCC PCS-201-010], Dermal Fibroblasts, Normal, Human Neonatal, Mitomycin C Treated [ATCC PCS-201-010]), Fibroblast Basal Medium must be supplemented with one of the following kits: Fibroblast Growth Kit–Serum-Free (ATCC PCS-201-040) Fibroblast Growth Kit–Low Serum (ATCC PCS-201-041) Optional media supplements: Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002) Phenol Red (ATCC PCS-999-001)
TypeMedia Sera Reagents
Volume480 mL

Specifications/Features

Storage:
2°C to 8°C

Contains:
essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts

Sterility:
Negative for bacteria, fungi and yeast.

Functional Testing:
Rate of proliferation and morphology.

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Induction of adipocyte thermogenic program by Rho-associated coiled-coil containing kinase 2iScienceApril 17, 2026Haw-Chih Tai, Xiaoou Li, Christina J. Park, Altayeb Alshaikh, Brian Yu, Daiqing Chen, Phetcharat Chen, James K. Liaored",Gibco,Cat# 11058021 Lipofectamine RNAiMAX,Invitrogen,Cat# 13778100 Fibroblast Basal Medium,ATCC,PCS-201-030 Fibroblast Growth Kit,ATCC,PCS-201-041 ,, Critical commercial assays,Critical commercial assays,Critical

(2) Fixed-Bed Bioreactor Culture Enhances Yield and Reparative Properties of hTERT Mesenchymal Stem Cell Extracellular VesiclesCellsApril 7, 2026Zachary Cuba, Lenny Godinho, Sujata Choudhury, Kajal Patil, Anastasia Williams, Weidong Zhou, Marissa Howard, Surya P. Aryal, Kevin A. Clayton, David A. Routenberg, et al.human primary dermal fibroblasts (ATCC® PCS-201-012™) were maintained in Fibroblast Basal Medium (ATCC® PCS-201-030™) supplemented with Fibroblast Growth Kit (ATCC® PCS-201-040™). Normal primary epidermal keratinocytes

(3) Protocol for generating an in vitro 3D multicellular culture model of ovarian high-grade serous carcinomaSTAR ProtocolsMarch 20, 2026Krister Wennerberg, Daria Bulanova, Laura Gall-Mas, Wojciech Senkowski, Lidia Moyano-GalceranCollagenase/hyaluronidase in DMEM,STEMCELL Technologies,Cat# 07912 Fibroblast Basal Medium,ATCC,Cat# PCS-201-030 Fibroblast Growth Kit-Low serum,ATCC,Cat# PCS-201-041 "Trypsin-EDTA (0.25%), phenol red",Thermo Fisher Scientific

(4) Novel Insights on Benzo[b]thiophene Analogues for MAO-B Inhibition and Neuroprotection: Design, Synthesis, Molecular Modelling Studies and Biological ActivityAntioxidants (Basel)March 10, 2026Francesca Arrighi, Emanuela Berrino, Paolo Guglielmi, Simone Carradori, Guya Diletta Marconi, Jacopo Pizzicannella, Simone Guarnieri, Tiziano Tuccinardi, Giulio Poli, Federico Pepi, et al.ATCC (PCS-201-018 ATCC, Manassas, VA, USA) and cultured with Fibroblast Basal Medium (485 mL; ATCC PCS-201-030) included in the growth kit (ATCC PCS-201-041) [47,48]. The human neuroblastoma cell line (SH-SY5Y)

(5) CRISPR/Cas-based activation of PPARGC1A boosts endogenous mitochondria and enhances cardiac function and recovery after myocardial infarctionMolecular TherapyMarch 9, 2026Mario Escobar, Saad A. Malik, Mira A. Srinivasa, Miguel A. Mendez-Sosa, Jessica M. Miller, Samantha L. Lydon, Sandy N. Luong, Pretty R. Mathew, Riham R.E. Abouleisa, Suridh Chakravarty, et al.μg/mL streptomycin; Fisher, 15-140-163). HDFa cells were cultured in Fibroblast Basal Medium (ATCC, PCS-201-030) supplemented with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) and 1% Pen/Strep. AC16 cells

(6) Mast Cell Chymase and Human Lung Fibroblast Interaction: Mechanisms and Implications for AsthmaInflammationFebruary 10, 2026Gunnar Pejler, Aida Paivandy, Fabio Rabelo Melo, Venkata Sita Rama Raju Allam, Agnes Öberg, Quan Wen, Peter Bergsten, Xinran O. ZhaoCollection (ATCC). These cells were cultured at 37 °C with 5% CO2 in Fibroblast Basal Medium (ATCC® PCS-201-030™) supplemented with Fibroblast Growth Kit-Low Serum (ATCC® PCS-201-041™), 100 U/mL penicillin, and 100

(7) Cathelicidin LL37-loaded extracellular vesicles from Edwardsiella piscicida promote antibacterial and wound-healing activitySci RepDecember 2, 2025Mawalle Kankanamge Hasitha Madhawa Dias, E. H. T. Thulshan Jayathilaka, Mahanama De Zoysaon the method described by Edirisinghe et al.51. The cells were cultured in fibroblast basal medium (PCS-201-030™, ATCC, Manassas, VA, USA) supplemented with the Fibroblast Growth Kit–Low Serum (PCS-201-041™, ATCC,

(8) Developing a therapeutic elastase that stimulates anti-tumor immunity by selectively killing cancer cellsCell Reports MedicineNovember 18, 2025Ravindra Gujar, Chang Cui, Maria Fumagalli, Nicole Martinez, Afshin Bahador, Alain Algazi, Kevin Harrington, Court Turner, Lev BeckerPCS-100-030 Endothelial Cell Growth Kit-BBE,ATCC,Cat# PCS-100-040 Fibroblast Basal Medium,ATCC,Cat# PCS-201-030 Fibroblast Growth Kit-Low serum,ATCC,Cat# PCS-201-040 Pen-Strep,Gibco,Cat#15140-122 Sodium Pyruvate,Gibco,Cat#11360-070 McCoy’s

(9) A Novel In Vitro Potency Assay Demonstrating the Anti-Fibrotic Mechanism of Action of CDCs in DeramiocelBiomedicinesOctober 29, 2025Yujia Li, Justin B. Nice, Marya Kozinova, Stephanie Adachi, Linda Marbán, Kristi Elliott, Minghao Sunrespectively. ATCC media to grow HDF and HCF were prepared according to manufacturer’s recommendations (ATCC/PCS-201-030 and ATCC/PCS-201-041, Manassas, VA, USA). Cells were maintained at 37 °C in a humidified atmosphere

(10) In Vitro and In Vivo Evaluation of a New Experimental Polydimethylsiloxane-Based Endodontic SealerJournal of Functional BiomaterialsOctober 28, 2025Fabiola Cardoso Maldonado, Cesar Gaitan Fonseca, Carlos Bermudez Jimenez, Luis Alejandro Aguilera Galaviz, Margarita L. Martinez-Fierro, Lorena Troncoso Vazquez, Martha Eugenia Reyes Ortizand cultured with the distributor's specifications y guidelines; ATCC® brand fibroblast basal medium (PCS-201-030; ATCC, Manassas, VA, USA), ATCC® brand fibroblast growth kit (PCS-201-040; ATCC, Manassas, VA, USA)

(11) Mechanical Cues Regulate Estrogen and Progesterone-Induced Nascent ECM Deposition by Human Endometrial Stromal CellsbioRxivOctober 17, 2025Grace K. Hinds, Arina Velieva, Yu-Chung Liu, Avinava Roy, Rima Chavali, Claudia LoebelATCC; 25-year-old donor) were cultured in fibroblast growth media, containing 10% fetal bovine serum (PCS-201-030 and PCS-201-041, ATCC), and passaged twice prior cryopreservation. All experiments were performed with

(12) Octopus minor Antimicrobial Peptide-Loaded Chitosan Nanoparticles Accelerate Dermal Wound HealingInt J Mol SciOctober 5, 2025Mawalle Kankanamge Hasitha Madhawa Dias, Shan Lakmal Edirisinghe, Mahanama De Zoysa, Ilson WhangMaterials and Methods 4.1. Raw Materials, Chemicals, and Reagents HDF (ATCC PCS-201-010™) cells and PCS-201-030™ (Fibroblast basal medium) were purchased from ATCC (Livingston, MT, USA). Fetal bovine serum (FBS)

(13) Spatial Single-Cell Atlas Reveals KSHV-Driven Broad Cellular Reprogramming, Progenitor Expansion, Immune and Vascular Remodeling in Kaposi’s SarcomabioRxivSeptember 11, 2025Wen Meng, Arun Das, Harsh Sinha, Rana Naous, Paige M. Bracci, Mike McGrath, Yufei Huang, Shou-Jiang GaoCell culture Primary human dermal fibroblasts (HDFa) (PCS-201–012; ATCC) were cultured in Fibroblast Basal Medium (PCS-201–030; ATCC) supplied with Fibroblast Growth Kit-Low serum (PCS-201–041; ATCC). KSHV-infected iSLK (iSLK-BAC16)

(14) Drug-induced metabolic remodeling of immune cell repertoire generates an effective broad-range antimicrobial effectResearch SquareJuly 30, 2025Bhupesh Kumar Prusty, Claudia Hollmann, Eun Chan Park, Zheng Liu, Faye Nourollahi, Georgy Nikolayshvili, Jonathan Dietz, Emils Bašēns, Mehul Vora, Trushnal Waghmare, et al.human foreskin fibroblasts (ATCC SCRC-1041) were maintained in primary fibroblast basal medium (ATCC PCS-201–030) supplemented with recommended growth factors and antibiotics (ATCC PCS-201-040). All cell lines were

(15) Radioactive Tracing of Testosterone Reveals Minimal Formation of 5α-DHT in SGBS Cells and Human Primary AdipocytesJ Endocr SocMay 16, 2025Andrea Andress Huacachino, Cátia F Marques, Clementina Mesaros, Trevor M Penningaccording to the ATCC protocol [28]. Briefly, cells were cultured with fibroblast basal medium (ATCC, PCS-201-030) containing growth kit-low serum (ATCC, PCS-201-041, lot: 80526241) (5 ng/mL recombinant human FGF-β

(16) Integrated analysis of transcriptional and metabolic responses to mitochondrial stressCell Reports MethodsApril 21, 2025Liam P. Kelley, Song-Hua Hu, Sarah A. Boswell, Peter K. Sorger, Alison E. Ringel, Marcia C. Haigisrecombinant proteins","Chemicals, peptides, and recombinant proteins" ,, Fibroblast Basal Medium,ATCC,PCS-201-030 Fibroblast Growth Kit-Low serum,ATCC,PCS-201-041 Trypsin-EDTA for Primary Cells,ATCC,PCS-999-003 Trypsin Neutralizing Solution

(17) A Human Cervix Chip for Preclinical Studies of Female Reproductive BiologyBio ProtocApril 5, 2025Zohreh Izadifar, Donald E. Ingber(LifeLine Cell Technology, catalog number: LL-0072) 5. Fibroblast basal medium (ATCC, catalog number: PCS-201-030) 6. Fibroblast Growth kit, low serum; includes L-glutamine rh FGF basic, rh insulin, hydrocortisone,

(18) Adipogenic Effect of Magnolol in Primary Human Pre-Adipocytes With Potential Skin Health and Volumizing EffectJ Cosmet DermatolMarch 26, 2025Alan D. Widgerow, Nava Dayan, Mary E. Ziegler, Faiza ShafiqCells were seeded at a density of 5000 cells/cm2 with complete medium: Fibroblast basal medium (Cat #: PCS‐201‐030, ATCC, Manassas, VA, USA.) supplemented with Fibroblast growth kit‐Low serum (Cat #: PCS‐201‐041, ATCC)

(19) A BCL-xL/BCL-2 PROTAC effectively clears senescent cells in the liver and reduces MASH-driven hepatocellular carcinoma in miceNat AgingMarch 1, 2025Yang Y, Jn-Simon N, He Y, Sun C, Zhang P, Hu W, Tian T, Zeng H, Basha S, Huerta AS, et al.SingleQuots supplements required for growth of RECs. PACs were cultured in fibroblast basal medium (Cat. No. PCS-201-030, ATCC) supplemented with fibroblast growth kit-low serum (Cat. No. PCS-201-041, ATCC). AML12 cells were

(20) G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migrationCell ReportsFebruary 25, 2025Liana C. Boraas, Mengwei Hu, Pieter Martino, Lauren Thornton, Charles E. Vejnar, Gang Zhen, Longhui Zeng, Dylan M. Parker, Andy L. Cox, Antonio J. Giraldez, et al.Pan-Cytokeratin, an epithelial marker, expression. HDFs were cultured in fibroblast growth medium (ATCC, PCS-201-030) supplemented with the Fibroblast Growth Kit-Low Serum (ATCC, PCS-201-041), 0.5 mL Phenol Red (ATCC,

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Fibroblast Basal Medium from ATCC

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Specifications/Features

ATCC

Citations (116)

Reviews

Fibroblast Basal Medium from ATCC

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