Description
The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) Kit utilizes engineered protein splicing elements (termed inteins) to purify recombinant proteins by a single column. This kit distinguishes itself from other protein fusion systems by its ability to separate a recombinant protein from the affinity tag without the use of a protease.
- Single-column purification without the use of proteases to remove the affinity tag
- Able to produce target protein without vector–derived amino acids
- Fusion to either C-terminus or N-terminus of target protein
- Isolation of proteins with or without an N-terminal methionine residue
- Ligation and labeling of recombinant proteins