GloMax Discover System from Promega

Product Specs
ItemGloMax Discover System
CompanyPromega
Price Supplier Page
Catalog NumberGM3000
QuantityEA
DetectionLuminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET
Measuring Time96-well plate: less than 1 minute; 384-well plate: less than 3 minutes
Plate Formats6, 12, 24, 48, 96 and 384-well plates
ApplicationsAbsorbance, Luminescence, Fluorescence, ELISA, BRET, FRET
Optical System9 absorbance filters
Wavelength(s)200 to 600 nm
Sample Type6- to 384-well plates

Specifications/Features

Temperature control, Shaking, Injectors

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) BTX-A51 treatment modulates host innate immune responses via NF-κB and β-catenin signaling during Rift Valley fever virus infection in vitroJournal of VirologyOctober 20, 2026Rafaela Flor, Timothy Stocker, Kaylee Petraccione, Maryna Akhrymuk, Brittany N. Heath, Ivan Akhrymuk, Caitlin M. Woodson, Kylene Kehn-Hallwas assessed via luminescence detection of ATP using a GloMax Discover plate reader (Promega, Cat No. GM3000). RT-qPCR Cell lysates were obtained using RNeasy Mini Kit RLT buffer (QIAGEN, Cat No. 74106), enriched

(2) Plant-Derived Senolytic Ingredient, Camellia sinensis Root Extract, Ameliorates Skin Aging-Associated PhenotypesExp DermatolOctober 13, 2026Sunyoung Park, Siyoung Cho, Daejin Min, Hyunjung Choi, Kyeonghwan Hwang, In Sup Kil, Won-Seok Park, Hyoung-June Kim(Promega, Madison, WI, USA) and quantified using a GloMax Discover Microplate Reader (catalogue number GM3000). 2.13 Cell Fractionation Cells were harvested and fractionated into cytosolic, mitochondrial and

(3) Animal-origin-free medium enhances G2/M progression while maintaining identity and genomic stability in human adipose-derived MSCsStem Cell Res TherOctober 5, 2026Su-Jin Lee, Chunggeon Lee, Eunji Kim, Min Hee Kang, Jae Woo Kang, Joo Youn Lee, Seyoung Ryu, Daeui Park, Hyang-Ae Leeinstructions. Optical density readings at 450 nm were acquired using a microplate absorbance reader (GloMax® Discover System; Promega, Madison, WI, USA). Data analysis was performed with GraphPad Prism software (v. 10.6.0) by

(4) The dual IGF-1R/IR blocker, Linsitinib, modulates human orbital fibroblast activity and proliferationFront ImmunolOctober 2, 2026Maximilian Luffy, Stefanie Wagner, Jan Wolf, Anna-Lena Ganz, Xaver Michels, Ryan Zeidan, Jeffrey D. Kent, Raymond S. Douglas, George J. Kahalystop solution. Absorbance was immediately measured at 450 nm using a GloMax Discover Microplate Reader GM3000 (Promega, Madison, Wisconsin, USA). AKT (pS473) phosphorylation levels were interpolated from the control

(5) Exercise alleviates age-related osteoarthritis associated with PGC-1α activation to suppress chondrocyte senescence and aberrant glycolysisJournal of Orthopaedic TranslationSeptember 22, 2026Yuting Cao, An Lao, Shijie Zhu, Fangchun Jin, Zhou Sha, Yu Zhuang, Huilu Zhan, Liao Wang, Huiwu Li, Kaili Lin, et al.were quantified by measuring relative light unit values with a luminometer (Promega, GloMax® Discover (GM3000), USA). 4.15 Cellular metabolic analysis The glycolytic capacity was measured using the Agilent Seahorse

(6) TRPV4-driven ATP release activates CFTR through ADORA2B and P2RY2 receptors in the airway epitheliumiScienceSeptember 18, 2026Michele Genovese, Martina De Santis, Alessandro Giordano, Pina Marotta, Luis J.V. GaliettaLuminescence was recorded every 30 s for 22 measurements using a GloMax® Discover Microplate Reader (GM3000, Promega). During readings, one epithelium received 15 μL of Coon’s-HEPES containing Eact (5 μM) whereas

(7) Structural basis of diverse antibody recognition of conserved coronavirus spike S2 epitopes that contribute to protective immunitybioRxivSeptember 16, 2026Adonis A. Rubio, Virginia Crivelli, Václav Hönig, Monika Čížková, Tomás Cervantes Cervantes Rincón, Morgan E. Abernathy, Megan K. Parada, Danielle Vahdat, Yu E. Lee, Michael Eso, et al.and Nanoluc Luciferase activity of lysates measured using the Nano-Glo Luciferase Assay System with GloMax Discover System reader (Promega). Luminescence units were relative to those derived from cells infected with SARS-CoV-2

(8) AGC kinase homology requires and enables co-targeting for CNS regenerationbioRxivSeptember 7, 2026Hassan Al-Ali, Abdiel Badillo-Martinez, Bassel Awada, Brian B. Silver, Omar S. Elwardany, Ronald N. Buckle, Graham Johnson, Siyuan Sun, Rodrigo Lendof, Nikhita Guhan, et al.NanoLuc Inhibitor were added according to manufacturer instructions, and BRET signal was measured on a GloMax Discover System (Promega). A four-parameter logistic model was fitted to the normalized data in GraphPad Prism (v11.0)

(9) Novel TBX20 Variations Susceptible to Sporadic Atrial FibrillationBiomedicinesSeptember 3, 2026Zhen-Yu Xu, Dao-Liang Zhang, Xing-Biao Qiu, Chen-Xi Yang, Ying-Jia Xu, Yi-Qing Yang, Ning LiDual-Glo® Luciferase Assay System (Cat. No. E2940; Promega, Madison, WI, USA), performed using the GloMax® Discover System in accordance with the manufacturer’s instructions. The transcriptional activities of the KCNH2 and

(10) Behaviour of iPSC-Derived and Donor-Derived Hepatic Stellate Cells in Organoid Cultures Is Highly Variable and Contributes to Fibrotic OutcomeCellsAugust 28, 2026Yuwei Yang, Mina Kazemzadeh Dastjerd, Ayla Smout, Vincent Merens, Burak Toprakhisar, Catherine M. Verfaillie, Christophe Chesné, Leo A. van van Grunsvenaccording to the supplier’s instructions. The luminescence signal was measured by a GloMax (Promega, GM3000). Raw luminescence values were normalized to the mean luminescence of the corresponding control group

(11) An Effector TaCRVP From Tuta Absoluta Oral Secretion Impairs H2O2-Mediated Plant Defense by Targeting Tomato SlCAT2Adv Sci (Weinh)August 25, 2026Jianquan Yan, Youdan Zhang, Zhujun Wang, Huatao Tang, Huiping Liu, Zi Chen, Shuai Liu, Jiayi Shi, Lu Peng, Youming Hou(Sigma‐Aldrich, A8511, SML2236). Luminescence was continuously recorded every 1.5 min using a GloMax Discover GM3000 Multimode Microplate Reader (Promega, USA). ROS production dynamics were represented as relative light

(12) Targeting PKGIα Cys42 attenuates cardiac dysfunction in heart failure with preserved ejection fractionSci AdvAugust 21, 2026Jie Su, Yue Zhao, Pierre Coleman, Xiaoping Yang, Mark Holt, Janice Raabe, Friederike Cuello, Ajay Shah, Michael J. Shattock, Min Zhang, et al.incubation, luminescence was measured using a GloMax Discover Microplate Reader (Promega, catalog no. GM3000). In some experiments, 10 ng of recombinant PKGIα was treated with or without 10 mM DTT for 20 min,

(13) Systematic investigation reveals extensive Epstein-Barr virus transcriptional regulation of the human genomeCell GenomicsAugust 12, 2026Phillip J. Dexheimer, Matthew R. Hass, Lee E. Edsall, Arame A. Diouf, Sydney H. Jones, Omer Donmez, Cailing Yin, Katelyn A. Dunn, Carmy Forney, Hayley K. Hesse, et al.Technologies,Cat#G2992A iBLOT,Invitrogen,Cat#IB21001 Odyssey Dlx Imaging System,LI-COR Biosciences,Cat#2645119 GloMax Discover,Promega Corporation,Cat#GM3000 QuantStudio6 Flex Real-Time PCR System,Thermo Fisher Scientific,Cat#4485692 CHEMIDOC Imaging System,Bio-Rad,Cat#12003153

(14) Cardiac function in zebrafish embryos is linked to an androgen receptor-adrenomedullin-proepicardium axisCell Commun SignalJuly 30, 2026Max Duong Phu, Alessandra Guerrero Samanidis, Sabrina Laibacher, Martina Burczyk, Yara Alkhars, Ana Janovic, Ashraf Al Madhoun, Ilona S. Skerjanc, Martin D. Burkhalter, Melanie Philippand renilla luciferase activity were measured using the Dual Glo Luciferase Assay system and a GloMax GM3000 plate-reader (both Promega). Readings were background subtracted using results from untransfected cells

(15) Spatial modulation of RAF by RAF/MEK glue enables full-dose combination with pan-RAF inhibitor and potent RAS-mutant tumor-selective MAPK and growth inhibitionbioRxivJuly 30, 2026Ana Orive-Ramos, Bijaya Gaire, Christos Adamopoulos, Beau Baars, Mathieu Desaunay, Ziyue Kou, Evangelia Matenoglou, Silvia Coma, Nayeli Gutiérrez-Trejo, Kevin Mohammed, et al.recommendations, and the absorbance was read at 450 nM using the GloMax® Discover microplate reader (Promega, GM3000). Western blot and immunoprecipitation Immunoblotting and immunoprecipitation were performed according

(16) Protocol for Measuring Drug–Target Engagement in Mouse Colorectal Cancer Organoids Using NanoBRET AssayBio ProtocJuly 20, 2026Hammed A. Badmos, Colin Steele, Ross Cagancontroller (Falcon, catalog number: 357804) 16. Glomax Discover plate reader (Promega, catalog number: GM3000) Procedure A. Generation of organoid-compatible plasmid vector A1. Design and synthesis of lentiviral

(17) Development of an in vivo, screenable, split-luciferase based model of huntingtin multimerizationiScienceJuly 17, 2026Morgan G. Thomas, Simon A. Levy, Meredith H. Jenkins, Morgan Lambert, Bess Frost(Sigma-Aldrich #Z721417) and transferred to the plate reader (GloMax Discover Microplate Reader Promega #GM3000). After allowing flies to recover from anesthesia for 30 min, luminescence signal from each well was

(18) Analysis of West Nile disease convalescents identifies human monoclonal antibodies protective against West Nile and related orthoflavivirusesImmunityJuly 14, 2026Tomás Cervantes Rincón, Tereza Frckova, Zaria I. Contejean, Jasmine Cantergiani, Kevin Groen, Benedetta Cena, Simone G. Moro, Filippo Bianchini, Luca Simonelli, David Jarrossay, et al.μL lysis buffer added (Promega, E1531). The Nano-Glo Luciferase Assay System (Promega, N1120) with GloMAx Discover System reader (Promega) were used to measure the NanoLuc luciferase activity of the lysate. Serum was diluted

(19) BET inhibition unmasks a targetable glycolytic dependency through a HIF1α stabilization and driven transcriptional program in a defined subset of triple-negative breast CancerCell Death DiscoveryJuly 8, 2026Teresa Rossi, Egidio Iorio, Mattea Chirico, Maria Elena Pisanu, Federica Torricelli, Alessia Ciarrocchi, Mariaelena Pistoni°C. Formazan crystals were dissolved in DMSO (100 μL/well), and absorbance was read at 560 nm using a GloMax Discover system (Promega). Data were normalized to the untreated control. Cell death analysis Cell death was assessed

(20) Multiplexed cytokine and antigen mRNA administration generates durable anti-tumor immunity against pancreatic cancerNature CommunicationsJune 19, 2026Chaitanya N. Parikh, Kelly D. DeMarco, Nikita Bhalerao, Hadiya K. Giwa, Griffin I. Kane, Ronnie W. Dinnell, Boyang Ma, Haruka Mori, Meghan L. Brassil, Katherine C. Murphy, et al.methanol, and absorbance was measured at 560 nm (OD₅₆₀) using a microplate reader (Promega Glomax, GM3000). Background signal from blank wells was subtracted to obtain final OD values. To assess the impact

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7/13/2026Works Great to Detect Absorbance and Fluorescence in 96 Well Plates Scott ScarneoThis machine is great to detect absorbance such as ELISA or fluorescence in ICC in 96 well plate layout. Assays are quick and easy to run and the set up is straight forward.