GloMax Discover System from Promega

Product Specs
ItemGloMax Discover System
CompanyPromega
Price Supplier Page
Catalog NumberGM3000
QuantityEA
DetectionLuminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET
Measuring Time96-well plate: less than 1 minute; 384-well plate: less than 3 minutes
Plate Formats6, 12, 24, 48, 96 and 384-well plates
ApplicationsAbsorbance, Luminescence, Fluorescence, ELISA, BRET, FRET
Optical System9 absorbance filters
Wavelength(s)200 to 600 nm
Sample Type6- to 384-well plates

Specifications/Features

Temperature control, Shaking, Injectors

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Protocol for ex vivo mouse paired single-gonad screening platform for phenotypical characterizationSTAR ProtocJune 19, 2026Martín Andrés Estermann, Karina Flores Rodriguez, James Hunnicutt, Humphrey Hung-Chang Yaobottom",ThermoFisher Scientific,Cat#136101 HD Camera,Leica,DMC6200 GloMax Discoverer microplate reader,Promega,GM3000 Tissue-Tek Cryomold 10 x 10 x 5 mm,Sakura,Cat#4565 Superfrost Plus Microscope Slides,ThermoFisher

(2) Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutationsMolecular Therapy OncologyJune 18, 2026Joshua Sammons, Jianguo Chen, Kari Thrasher, Lianwu Fu, Ming Du, Hui Wen, J. Robert Bostwick, Paige Vinson, Omar Moukha-Chafiq, Corinne Augelli-Szafran, et al.perform the NanoLuc assay with the Nano-Glo luciferase assay system (Promega, Cat#N1120) using the GloMax Discover System (Promega). The lysate protein concentration was measured with the Bio-Rad Protein Assay Dye Reagent Concentrate

(3) Signatures in the Protein Content of Human and Murine Blood Serum Exosomes, in the Context of Major Depressive Disorder, Are Associated with Cytokine ActivityCellsJune 6, 2026Jorge Manuel Vásquez-Pérez, Mónica Flores-Ramos, María Del Pilar Ramos-Godínez, Gerardo Bernabé Ramírez-Rodríguezinstructions. The 96-well plates were read in a Promega ELISA reader (Glomax Discover, Madison, WI, USA Ref#GM3000) [61,62,63]. 2.3.2. Evaluation of the Coat State (CS) and Weight of Mice Coat condition is described

(4) Rho2-dependent cell wall remodeling boosts the fungistatic activity of manogepix against Aspergillus fumigatusThe Cell SurfaceJune 1, 2026Sean Brazil, Isabel Kopp-Fouquet, Samuel Zen Kai Yap, Michael Carty, Johannes Wageneradditional drug. Luminescence was measured over time at 37 °C with a Glomax discover microplate reader (#GM3000; Promega). 4.4 Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) RT-qPCR

(5) Underrecognized Tick-Borne Encephalitis in Serbia: Evidence from Patients with Suspected West Nile Virus Neuroinvasive DiseasePathogensMay 29, 2026Dragana Mijatović, Ana Marija Radevska, Dejan Jakimovski, Lidija Popović-Dragonjić, Biljana Popovska Jovičić, Jagoda Gavrilović, Siniša Sević, Dajana Lendak, Irina Stojanac, Alejandro Cabezas-Cruz, et al.measured using the Nano-Glo Luciferase Assay System (Promega, Madison, WI, USA, Cat. No N1120) on a GloMax Discover System reader (Promega, Madison, WI, USA). Serum samples were tested at a 1:100 dilution. 2.2.3. TBEV Microneutralization

(6) Transition to fetal calf serum-free culture enhances kidney proximal tubule cell bioenergetics and allows pharmacological applicationsCell Tissue ResMay 23, 2026Thomas K. van der Made, Pim Cleijpool, Polly Paul, Devon A. Barnes, Stefan Oswald, Bonnie C. Broeksma, Quentin Faucher, Silvia M. Mihăilă, Rosalinde Masereeuwemission) was measured using GloMax® Discover Microplate Reader (Promega, Leiden, The Netherlands, GM3000). Remaining solution was aspirated; cells were washed once with HBSS and the fresh respective medium

(7) NFAT5: A Metabolic Time Capsule Encoding the History of Paternal Metabolic Oxidative Stress Within the Male Reproductive TractAntioxidants (Basel)May 20, 2026Nicola Mosca, Antonella Migliaccio, Teresa Chioccarelli, Donato Cappetta, Antonella De Angelis, Marialucia Telesca, Liberato Berrino, Danila Valletta, Alice Luddi, Chiara Donati, et al.incubation at 37 °C, the absorbance was measured at 450 nm with a reference wavelength of 650 nm using the GloMAX discover system (Promega, Madison, WI, USA). 2.15. Nuclear and Cytoplasmic Protein Extraction Protein samples were

(8) Combined multi-omics and multi-spectral profiling of plasma extracellular vesicles reveals liquid biopsy biomarkers for glioma diagnosisCell Reports MedicineMay 19, 2026Stephen David Robinson, Biniam Tsegay Haile, Matthew Reily-Bell, Olivia Iwanowytsch, Siobhan Palmer, Dorte Schou Nørøxe, Panagiota S. Filippou, Joanna Renaut, Alan Lazarus, Georgios Antoniou, et al.4K camera",JEOL,N/A JEOL 2200FS microscope with a C100 imager,JEOL,N/A GloMax Plate Reader,Promega,GM3000 SpectraMax Microplate Reader,Molecular devices,N/A iBlot 2 System,Invitrogen,15217995 UVP Chemstudio

(9) Application of a Blood-Brain Barrier Organ-on-a-Chip Model for Assessment of Countermeasure Efficiency Against Eastern Equine Encephalitis VirusVirusesMay 9, 2026Niloufar A. Boghdeh-Olson, Michael D. Barrera, Clayton M. Britt, David K. Schaffer, Jacquelyn A. Brown, John P. Wikswo, Aarthi Narayananmeasuring and comparing the fluorescence intensity using GloMax Microplate Reader by Promega (Promega, Cat. GM3000, Madison, WI, USA). In all figures, yellow arrows represent vascular side media or perfused supernatants

(10) Biallelic MCUR1 nonsense mutation associated with vacuolar myopathy and altered mitochondrial calcium signalingActa Neuropathol CommunMay 5, 2026Anna Maria Haschke, Anja von Renesse, Eugenio Graceffo, Susanne Morales-Gonzalez, Alessandro Prigione, Christoph Hübner, Werner Stenzel, Markus SchuelkeDMNPE-caged Luciferin (Abcam, ab145163) we measured bioluminescence using the GloMax™ plate reader (Promega, GM3000). Data was normalized to the number of cells evaluated by CyQUANT™ (Invitrogen, #C35011). Intracellular

(11) A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targetsHemasphereApril 23, 2026Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson, et al.Dual‐Glo Luciferase Assay System (Promega Cat# E2920) and a GloMax Discover MicroPlate Reader (Promega Cat# GM3000) according to the manufacturer's instructions. Bioinformatic analysis of patient samples RNA sequencing

(12) Establishment of a green fluorescent protein (GFP)-based reporter for picornaviral 3C proteasesJournal of VirologyApril 21, 2026Junki Hirano, Tsuyoshi Hayashi, Yuichi Someya, Kazuma Okada, Kentaro Uemura, Ming Te Yeh, Chikako Ono, Shuhei Taguwa, Yoshiharu Matsuuraoptimized the flipGFP-based reporter to a 96-well format that is measurable by a conventional plate reader (GloMax Discover System, Promega) and assessed whether the assay could be adapted to the screening of 3C protease inhibitors

(13) Indirect inhibition of the NLRP3-interleukin-1β axis contributes to the efficacy of JAK1 inhibitors in experimental colitis and human ulcerative colitisNature CommunicationsApril 18, 2026Beibei Liu, Marianne R. Spalinger, Annalisa Invernizzi, Eike Gerdes, Babett Steglich, Marlene Schwarzfischer, Andres Machicote, Penelope Pelczar, Doris Pöhlmann, Mikolaj Nawrocki, et al.thereby decreasing the acceptor signal. Measurements were performed on a GloMax® Discover (Promega, Cat#GM3000) luminometer. Occupancy of NLRP3 by inhibitors was calculated based on the detected signals and formula

(14) Shengdihuang Xiyangshen Zhimu Cangzhu Formula Improves Hepatic Glycogen Synthesis via the JNK/c-Jun/IRS1/GSK3β Signaling PathwayJ Diabetes ResApril 15, 2026Kewen Qu, Mei Li, Jinxu Sun, Shangping Wu, Xinyu Liu, Kanglin Liu, Shuxin Wu, Xiangyu Guonm for each well was measured using an enzyme label analyzer (Promega GloMax Discover System, USA, GM3000). 2.4. Liver Histopathology 2.4.1. Hematoxylin–Eosin (HE) Staining Mice liver tissues were fixed

(15) Phosphorothioate antisense oligonucleotide induced innate immune activation is attenuated by tryptophan oxidation productsNucleic Acids ResApril 13, 2026Julia Pytte, Lesley Saldana, Wei Zhang, Konstantina Skourti-Stathaki, Stanley T Crookewhite 384-well plates. Luminescence was measured using the GloMax Discover Microplate Reader (Promega, GM3000). ELISA assays (Immunsol) for quantification of secreted Kyn and Trp were conducted according to the

(16) Enhanced Antitumor Response in Breast Cancer via Parthanatos Activation Mediated by the Synergistic Effect of Etoposide and ResveratrolCurr Issues Mol BiolApril 7, 2026Negar Taghavi Pourianazar, Narin Abdullahwas transferred to a 96-well plate for luminescence detection using a microplate luminometer (Promega GloMax Discover System, Madison, WI, USA). With background subtraction, the values were normalized to the individual control group

(17) Human antibodies against West Nile and related orthoflavivirusesbioRxivApril 6, 2026Tomás Cervantes Cervantes Rincón, Tereza Frckova, Zaria I. Contejean, Jasmine Cantergiani, Kevin Groen, Benedetta Cena, Simone G. Moro, Filippo Bianchini, Luca Simonelli, David Jarrossay, et al.μL lysis buffer added (Promega, E1531). The Nano-Glo Luciferase Assay System (Promega, N1120) with GloMAx Discover System reader (Promega) were used to measure the NanoLuc luciferase activity of the lysate. Serum was diluted

(18) Sichuan pepper, Zanthoxylum bungeanum Maxim., suppresses human coronavirus OC43 infection by inhibiting viral entry and impairing autolysosome accumulationChin MedApril 2, 2026Hyeonjeong Park, In Jin Ha, Young-Hee Jin, Sunoh KwonMTS-based assay (Promega Corporation, Madison, WI, USA). Absorbance was detected at 490 nm using a GM3000 GloMax® Discover Microplate Reader (Promega Corporation). To assess viability, the absorbance values

(19) Structure-functionality relationship of collagen-fibrin interpenetrating hydrogels for engineered tumor-stroma modelsActa BiomaterialiaApril 1, 2026Sae Rome Choi, Seamus M. Mellican, Tyler J. Roberts, Sehong Kang, Bennett D. Elzey, Hyunjoon Kong, Chelsea S. Davis, Melissa L. Fishel, Matthew J. Flick, Alisa S. Wolberg, et al.different tissue constructs in a pre-warmed 96-well plate at 37 °C. Absorbance at 405 nm was measured using GloMax Discover System (Promega Corporation, WI) in 30 sec intervals for 80 min at 37 °C. 2.7. Cancer-associated fibroblast

(20) Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interactionCell DiscoveryMarch 31, 2026Yi-yang Zhao, Yi-hui Wang, Zi-han Li, Dong-yuan Chang, Lin Nie, Ming-hui Zhao, Sydney Chi Wai Tang, Min Chenof 0.01 g. Fasting blood glucose, total cholesterol, and triglyceride levels were quantified via a GM3000 Chemistry Analyzer (Promega Biotech Co., Ltd.). The urinary albumin concentration was determined with

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