GloMax Discover System from Promega

Product Specs
ItemGloMax Discover System
CompanyPromega
Price Supplier Page
Catalog NumberGM3000
QuantityEA
DetectionLuminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET
Measuring Time96-well plate: less than 1 minute; 384-well plate: less than 3 minutes
Plate Formats6, 12, 24, 48, 96 and 384-well plates
ApplicationsAbsorbance, Luminescence, Fluorescence, ELISA, BRET, FRET
Optical System9 absorbance filters
Wavelength(s)200 to 600 nm
Sample Type6- to 384-well plates

Specifications/Features

Temperature control, Shaking, Injectors

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Cardiac function in zebrafish embryos is linked to an androgen receptor-adrenomedullin-proepicardium axisCell Commun SignalJuly 30, 2026Max Duong Phu, Alessandra Guerrero Samanidis, Sabrina Laibacher, Martina Burczyk, Yara Alkhars, Ana Janovic, Ashraf Al Madhoun, Ilona S. Skerjanc, Martin D. Burkhalter, Melanie Philippand renilla luciferase activity were measured using the Dual Glo Luciferase Assay system and a GloMax GM3000 plate-reader (both Promega). Readings were background subtracted using results from untransfected cells

(2) Protocol for Measuring Drug–Target Engagement in Mouse Colorectal Cancer Organoids Using NanoBRET AssayBio ProtocJuly 20, 2026Hammed A. Badmos, Colin Steele, Ross Cagancontroller (Falcon, catalog number: 357804) 16. Glomax Discover plate reader (Promega, catalog number: GM3000) Procedure A. Generation of organoid-compatible plasmid vector A1. Design and synthesis of lentiviral

(3) Development of an in vivo, screenable, split-luciferase based model of huntingtin multimerizationiScienceJuly 17, 2026Morgan G. Thomas, Simon A. Levy, Meredith H. Jenkins, Morgan Lambert, Bess Frost(Sigma-Aldrich #Z721417) and transferred to the plate reader (GloMax Discover Microplate Reader Promega #GM3000). After allowing flies to recover from anesthesia for 30 min, luminescence signal from each well was

(4) Analysis of West Nile disease convalescents identifies human monoclonal antibodies protective against West Nile and related orthoflavivirusesImmunityJuly 14, 2026Tomás Cervantes Rincón, Tereza Frckova, Zaria I. Contejean, Jasmine Cantergiani, Kevin Groen, Benedetta Cena, Simone G. Moro, Filippo Bianchini, Luca Simonelli, David Jarrossay, et al.μL lysis buffer added (Promega, E1531). The Nano-Glo Luciferase Assay System (Promega, N1120) with GloMAx Discover System reader (Promega) were used to measure the NanoLuc luciferase activity of the lysate. Serum was diluted

(5) Protocol for ex vivo mouse paired single-gonad screening platform for phenotypical characterizationSTAR ProtocJune 19, 2026Martín Andrés Estermann, Karina Flores Rodriguez, James Hunnicutt, Humphrey Hung-Chang Yaobottom",ThermoFisher Scientific,Cat#136101 HD Camera,Leica,DMC6200 GloMax Discoverer microplate reader,Promega,GM3000 Tissue-Tek Cryomold 10 x 10 x 5 mm,Sakura,Cat#4565 Superfrost Plus Microscope Slides,ThermoFisher

(6) Multiplexed cytokine and antigen mRNA administration generates durable anti-tumor immunity against pancreatic cancerNature CommunicationsJune 19, 2026Chaitanya N. Parikh, Kelly D. DeMarco, Nikita Bhalerao, Hadiya K. Giwa, Griffin I. Kane, Ronnie W. Dinnell, Boyang Ma, Haruka Mori, Meghan L. Brassil, Katherine C. Murphy, et al.methanol, and absorbance was measured at 560 nm (OD₅₆₀) using a microplate reader (Promega Glomax, GM3000). Background signal from blank wells was subtracted to obtain final OD values. To assess the impact

(7) Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutationsMolecular Therapy OncologyJune 18, 2026Joshua Sammons, Jianguo Chen, Kari Thrasher, Lianwu Fu, Ming Du, Hui Wen, J. Robert Bostwick, Paige Vinson, Omar Moukha-Chafiq, Corinne Augelli-Szafran, et al.perform the NanoLuc assay with the Nano-Glo luciferase assay system (Promega, Cat#N1120) using the GloMax Discover System (Promega). The lysate protein concentration was measured with the Bio-Rad Protein Assay Dye Reagent Concentrate

(8) Signatures in the Protein Content of Human and Murine Blood Serum Exosomes, in the Context of Major Depressive Disorder, Are Associated with Cytokine ActivityCellsJune 6, 2026Jorge Manuel Vásquez-Pérez, Mónica Flores-Ramos, María Del Pilar Ramos-Godínez, Gerardo Bernabé Ramírez-Rodríguezinstructions. The 96-well plates were read in a Promega ELISA reader (Glomax Discover, Madison, WI, USA Ref#GM3000) [61,62,63]. 2.3.2. Evaluation of the Coat State (CS) and Weight of Mice Coat condition is described

(9) Rho2-dependent cell wall remodeling boosts the fungistatic activity of manogepix against Aspergillus fumigatusThe Cell SurfaceJune 1, 2026Sean Brazil, Isabel Kopp-Fouquet, Samuel Zen Kai Yap, Michael Carty, Johannes Wageneradditional drug. Luminescence was measured over time at 37 °C with a Glomax discover microplate reader (#GM3000; Promega). 4.4 Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) RT-qPCR

(10) AI‐Designed Cyclic Peptides Enable Controllable Modulation of the CD28 Immune CheckpointAdvanced ScienceMay 30, 2026Katarzyna Kuncewicz, Saurabh Upadhyay, Renjie Zhu, Hongliang Duan, Moustafa T. Gabr(Promega) was added according to the manufacturer's instructions, and luminescence was measured using a GloMax Discover System (Promega). Dose‐response curves were generated using GraphPad Prism 10 by fitting data to a four‐parameter

(11) Underrecognized Tick-Borne Encephalitis in Serbia: Evidence from Patients with Suspected West Nile Virus Neuroinvasive DiseasePathogensMay 29, 2026Dragana Mijatović, Ana Marija Radevska, Dejan Jakimovski, Lidija Popović-Dragonjić, Biljana Popovska Jovičić, Jagoda Gavrilović, Siniša Sević, Dajana Lendak, Irina Stojanac, Alejandro Cabezas-Cruz, et al.measured using the Nano-Glo Luciferase Assay System (Promega, Madison, WI, USA, Cat. No N1120) on a GloMax Discover System reader (Promega, Madison, WI, USA). Serum samples were tested at a 1:100 dilution. 2.2.3. TBEV Microneutralization

(12) Transition to fetal calf serum-free culture enhances kidney proximal tubule cell bioenergetics and allows pharmacological applicationsCell Tissue ResMay 23, 2026Thomas K. van der Made, Pim Cleijpool, Polly Paul, Devon A. Barnes, Stefan Oswald, Bonnie C. Broeksma, Quentin Faucher, Silvia M. Mihăilă, Rosalinde Masereeuwemission) was measured using GloMax® Discover Microplate Reader (Promega, Leiden, The Netherlands, GM3000). Remaining solution was aspirated; cells were washed once with HBSS and the fresh respective medium

(13) NFAT5: A Metabolic Time Capsule Encoding the History of Paternal Metabolic Oxidative Stress Within the Male Reproductive TractAntioxidants (Basel)May 20, 2026Nicola Mosca, Antonella Migliaccio, Teresa Chioccarelli, Donato Cappetta, Antonella De Angelis, Marialucia Telesca, Liberato Berrino, Danila Valletta, Alice Luddi, Chiara Donati, et al.incubation at 37 °C, the absorbance was measured at 450 nm with a reference wavelength of 650 nm using the GloMAX discover system (Promega, Madison, WI, USA). 2.15. Nuclear and Cytoplasmic Protein Extraction Protein samples were

(14) Combined multi-omics and multi-spectral profiling of plasma extracellular vesicles reveals liquid biopsy biomarkers for glioma diagnosisCell Reports MedicineMay 19, 2026Stephen David Robinson, Biniam Tsegay Haile, Matthew Reily-Bell, Olivia Iwanowytsch, Siobhan Palmer, Dorte Schou Nørøxe, Panagiota S. Filippou, Joanna Renaut, Alan Lazarus, Georgios Antoniou, et al.4K camera",JEOL,N/A JEOL 2200FS microscope with a C100 imager,JEOL,N/A GloMax Plate Reader,Promega,GM3000 SpectraMax Microplate Reader,Molecular devices,N/A iBlot 2 System,Invitrogen,15217995 UVP Chemstudio

(15) Application of a Blood-Brain Barrier Organ-on-a-Chip Model for Assessment of Countermeasure Efficiency Against Eastern Equine Encephalitis VirusVirusesMay 9, 2026Niloufar A. Boghdeh-Olson, Michael D. Barrera, Clayton M. Britt, David K. Schaffer, Jacquelyn A. Brown, John P. Wikswo, Aarthi Narayananmeasuring and comparing the fluorescence intensity using GloMax Microplate Reader by Promega (Promega, Cat. GM3000, Madison, WI, USA). In all figures, yellow arrows represent vascular side media or perfused supernatants

(16) Biallelic MCUR1 nonsense mutation associated with vacuolar myopathy and altered mitochondrial calcium signalingActa Neuropathol CommunMay 5, 2026Anna Maria Haschke, Anja von Renesse, Eugenio Graceffo, Susanne Morales-Gonzalez, Alessandro Prigione, Christoph Hübner, Werner Stenzel, Markus SchuelkeDMNPE-caged Luciferin (Abcam, ab145163) we measured bioluminescence using the GloMax™ plate reader (Promega, GM3000). Data was normalized to the number of cells evaluated by CyQUANT™ (Invitrogen, #C35011). Intracellular

(17) A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targetsHemasphereApril 23, 2026Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson, et al.Dual‐Glo Luciferase Assay System (Promega Cat# E2920) and a GloMax Discover MicroPlate Reader (Promega Cat# GM3000) according to the manufacturer's instructions. Bioinformatic analysis of patient samples RNA sequencing

(18) Establishment of a green fluorescent protein (GFP)-based reporter for picornaviral 3C proteasesJournal of VirologyApril 21, 2026Junki Hirano, Tsuyoshi Hayashi, Yuichi Someya, Kazuma Okada, Kentaro Uemura, Ming Te Yeh, Chikako Ono, Shuhei Taguwa, Yoshiharu Matsuuraoptimized the flipGFP-based reporter to a 96-well format that is measurable by a conventional plate reader (GloMax Discover System, Promega) and assessed whether the assay could be adapted to the screening of 3C protease inhibitors

(19) Indirect inhibition of the NLRP3-interleukin-1β axis contributes to the efficacy of JAK1 inhibitors in experimental colitis and human ulcerative colitisNature CommunicationsApril 18, 2026Beibei Liu, Marianne R. Spalinger, Annalisa Invernizzi, Eike Gerdes, Babett Steglich, Marlene Schwarzfischer, Andres Machicote, Penelope Pelczar, Doris Pöhlmann, Mikolaj Nawrocki, et al.thereby decreasing the acceptor signal. Measurements were performed on a GloMax® Discover (Promega, Cat#GM3000) luminometer. Occupancy of NLRP3 by inhibitors was calculated based on the detected signals and formula

(20) A poorly reactogenic lipid nanoparticle-mRNA vaccine unveils an innate immune pathway for adverse reactionsnpj VaccinesApril 16, 2026Tomohiro Takano, Keigo Kumagai, Hitoshi Iuchi, Kazutaka Terahara, Aya Mizuike, Eita Sasaki, Yu Adachi, Ryutaro Kotaki, Saya Moriyama, Shinichiro Ota, et al.Bright-Glo Luciferase Assay System (Promega, #E2610) on a GloMax Discover microplate reader (Promega, #GM3000). IC₅₀ values were calculated using Prism 10 (GraphPad). Titers below the detection limit (IC₅₀ = 20)

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