GloMax Discover System from Promega

Product Specs
ItemGloMax Discover System
CompanyPromega
Price Supplier Page
Catalog NumberGM3000
QuantityEA
DetectionLuminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET
Measuring Time96-well plate: less than 1 minute; 384-well plate: less than 3 minutes
Plate Formats6, 12, 24, 48, 96 and 384-well plates
ApplicationsAbsorbance, Luminescence, Fluorescence, ELISA, BRET, FRET
Optical System9 absorbance filters
Wavelength(s)200 to 600 nm
Sample Type6- to 384-well plates

Specifications/Features

Temperature control, Shaking, Injectors

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutationsMolecular Therapy OncologyJune 18, 2026Joshua Sammons, Jianguo Chen, Kari Thrasher, Lianwu Fu, Ming Du, Hui Wen, J. Robert Bostwick, Paige Vinson, Omar Moukha-Chafiq, Corinne Augelli-Szafran, et al.perform the NanoLuc assay with the Nano-Glo luciferase assay system (Promega, Cat#N1120) using the GloMax Discover System (Promega). The lysate protein concentration was measured with the Bio-Rad Protein Assay Dye Reagent Concentrate

(2) Rho2-dependent cell wall remodeling boosts the fungistatic activity of manogepix against Aspergillus fumigatusThe Cell SurfaceJune 1, 2026Sean Brazil, Isabel Kopp-Fouquet, Samuel Zen Kai Yap, Michael Carty, Johannes Wageneradditional drug. Luminescence was measured over time at 37 °C with a Glomax discover microplate reader (#GM3000; Promega). 4.4 Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) RT-qPCR

(3) A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targetsHemasphereApril 23, 2026Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson, et al.Dual‐Glo Luciferase Assay System (Promega Cat# E2920) and a GloMax Discover MicroPlate Reader (Promega Cat# GM3000) according to the manufacturer's instructions. Bioinformatic analysis of patient samples RNA sequencing

(4) Establishment of a green fluorescent protein (GFP)-based reporter for picornaviral 3C proteasesJournal of VirologyApril 21, 2026Junki Hirano, Tsuyoshi Hayashi, Yuichi Someya, Kazuma Okada, Kentaro Uemura, Ming Te Yeh, Chikako Ono, Shuhei Taguwa, Yoshiharu Matsuuraoptimized the flipGFP-based reporter to a 96-well format that is measurable by a conventional plate reader (GloMax Discover System, Promega) and assessed whether the assay could be adapted to the screening of 3C protease inhibitors

(5) Shengdihuang Xiyangshen Zhimu Cangzhu Formula Improves Hepatic Glycogen Synthesis via the JNK/c-Jun/IRS1/GSK3β Signaling PathwayJ Diabetes ResApril 15, 2026Kewen Qu, Mei Li, Jinxu Sun, Shangping Wu, Xinyu Liu, Kanglin Liu, Shuxin Wu, Xiangyu Guonm for each well was measured using an enzyme label analyzer (Promega GloMax Discover System, USA, GM3000). 2.4. Liver Histopathology 2.4.1. Hematoxylin–Eosin (HE) Staining Mice liver tissues were fixed

(6) Phosphorothioate antisense oligonucleotide induced innate immune activation is attenuated by tryptophan oxidation productsNucleic Acids ResApril 13, 2026Julia Pytte, Lesley Saldana, Wei Zhang, Konstantina Skourti-Stathaki, Stanley T Crookewhite 384-well plates. Luminescence was measured using the GloMax Discover Microplate Reader (Promega, GM3000). ELISA assays (Immunsol) for quantification of secreted Kyn and Trp were conducted according to the

(7) Enhanced Antitumor Response in Breast Cancer via Parthanatos Activation Mediated by the Synergistic Effect of Etoposide and ResveratrolCurr Issues Mol BiolApril 7, 2026Negar Taghavi Pourianazar, Narin Abdullahwas transferred to a 96-well plate for luminescence detection using a microplate luminometer (Promega GloMax Discover System, Madison, WI, USA). With background subtraction, the values were normalized to the individual control group

(8) Human antibodies against West Nile and related orthoflavivirusesbioRxivApril 6, 2026Tomás Cervantes Cervantes Rincón, Tereza Frckova, Zaria I. Contejean, Jasmine Cantergiani, Kevin Groen, Benedetta Cena, Simone G. Moro, Filippo Bianchini, Luca Simonelli, David Jarrossay, et al.μL lysis buffer added (Promega, E1531). The Nano-Glo Luciferase Assay System (Promega, N1120) with GloMAx Discover System reader (Promega) were used to measure the NanoLuc luciferase activity of the lysate. Serum was diluted

(9) Sichuan pepper, Zanthoxylum bungeanum Maxim., suppresses human coronavirus OC43 infection by inhibiting viral entry and impairing autolysosome accumulationChin MedApril 2, 2026Hyeonjeong Park, In Jin Ha, Young-Hee Jin, Sunoh KwonMTS-based assay (Promega Corporation, Madison, WI, USA). Absorbance was detected at 490 nm using a GM3000 GloMax® Discover Microplate Reader (Promega Corporation). To assess viability, the absorbance values

(10) Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interactionCell DiscoveryMarch 31, 2026Yi-yang Zhao, Yi-hui Wang, Zi-han Li, Dong-yuan Chang, Lin Nie, Ming-hui Zhao, Sydney Chi Wai Tang, Min Chenof 0.01 g. Fasting blood glucose, total cholesterol, and triglyceride levels were quantified via a GM3000 Chemistry Analyzer (Promega Biotech Co., Ltd.). The urinary albumin concentration was determined with

(11) Pathogenic variants in the cohesin loader subunit MAU2 underlie a distinct Cornelia de Lange Syndrome subtypeNat CommunMarch 30, 2026Ilaria Parenti, Alina Hesters, Marta Gil-Salvador, Laura Duffy, Deniz Kanber, Jasmin Beygo, Jennifer Kerkhof, Laura Steenpaß, Elsa Leitão, Julia Woestefeld, et al.measured 24 h post-transfection with the Dual Luciferase Reporter Assay System (E1980, Promega) using the GloMax Discover System (Promega). All measurements were performed in triplicate in at least six independent experiments. Relative

(12) SMR Peptide Modulates Tumor-Derived Extracellular Vesicles microRNA and Inflammatory Transcript Signatures in TNBCCellsMarch 19, 2026Ming-Bo Huang, Fengxia Yan, Uswa Jadoon, Jennifer Y. Wu, Dara Brena, Erica L. Johnson, Jonathan Stiles, Lily Yang, Brian M. Rivers, Vincent C. BondMadison, WI, USA) according to the manufacturer’s instructions. Luminescence was measured using the GloMax® Discover System. For microRNA profiling, RNA samples were analyzed using nCounter® miRNA Expression Panels (NanoString

(13) Umbelliferone Inhibits Zika Virus (ZIKV) NS5 Activity and Replication In VitroACS OmegaMarch 17, 2026Ji-Wan Choi, Yoon-Jae Songaddition of 0.25 μM SYTO 9 and stabilization for 5 min at room temperature. Fluorescence was measured on a GloMax Discover System (Promega) at excitation/emission settings of 475 nm/500–550 nm. Supplementary Material

(14) Fimepinostat Promotes Apoptosis and Decreases Cytokine Secretion in NF2-Related Human Schwannoma CellsInt J Mol SciMarch 13, 2026Anna Nagel, Ethan W. Hass, Hollie Hayes, Lenna Huelbes, Sofia Oliveira, Haley M. Hardin, Mikhail Marasigan, Eric Nisenbaum, Carly Misztal, Fred F. Telischi, et al.viability using CellTiter-Glo 2.0 (Promega). Relative luminescence units (RLUs) were measured using the Glomax Discover System (Promega). Data were analyzed using SAS Software (v9.4). 4.3. Sciatic Nerve Allograft Study Twenty

(15) Complement Activation May Drive the Pathogenicity of Anti-α6 and Anti-β4 Integrin Antibodies In VivoBiomoleculesMarch 12, 2026Gefei Du, Shirin Emtenani, Dennis Niese, Jian Liu, Ferdinand Gebauer, Neele J. Dunst, Aysun Gökce, Kristina Spaniol, Florian Groeber-Becker, Jelena Šimunović, et al.were seeded into each well, and the chemiluminescence reaction was monitored over 2 h at 37 °C using a GloMax® Discover System microplate reader (Promega, Mannheim, Germany). Statistical significance was assessed using the Kruskal–Wallis

(16) Systematic functional validation of IKAROS variants from patients and laboratory-generated mutationsBlood AdvancesMarch 10, 2026Hye Sun Kuehn, Agustin Alejandro Gil Silva, Natchanun Klangkalya, Ana Esteve-Sole, Shubham Goel, Julie E. Niemela, Jennifer L. Stoddard, Hey Chong, Kelli W Williams, Winnie Ip, et al.activity was measured using the Secrete-Pair Dual Luminescence Assay Kit (GeneCopoeia) on a Glomax GM3000 plate reader (Promega). Statistical analysis When indicated, data were analyzed using 2-tailed Student

(17) CRISPR/Cas9 screening with destabilized bicistronic fluorescent protein reporter revealed PABPN1 as a hub of regulators for alternative polyadenylationNucleic Acids ResearchFebruary 27, 2026Zhijie Hu, Yuchi Liu, Yuting Han, Mengxia Li, Ke Deng, Xin Lu, Yingye Huang, Cheng Liang, Yonghua Wang, Yonggui Fu, et al.(hluc) luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Vazyme) on a GloMax Discover System (Promega) according to the manufacturers’ instructions. Cellular proliferation analysis SW620 and

(18) Cellular signaling pathway of Shiga toxin-induced ATP releaseFrontiers in Cellular and Infection MicrobiologyFebruary 23, 2026Karl Johansson, Ida Arvidsson, Markus Wendler, Ann-Charlotte Kristoffersson, Diana Karpmanin HBSS, and the ATP content was determined by luminescence measurement at 1s integration time in a Glomax Discover System (Promega, Madison, WI). Experiments in which Stx1 did not increase ATP release (8/43) were excluded

(19) CHI3L1 Expression in Chordoma: Implications for Immunotherapeutic InterventionCellsFebruary 22, 2026Beatrice Campilan, Christian Godinez, Jonathan Arditi, Jessica Ding, Kaylee Gallagher, Andrew Fogarty, Christian Schroeder, Madison J. Michles, Weston de Lomba, Joselynn Wallace, et al.temperature. After stop solution was added, the plate was read on the Glomax Discover Microplate Reader (Cat. GM3000) from Promega (Madison, WI, USA). 3. Results 3.1. Transcriptomics The transition between primary

(20) Double Knockdown of the Androgen Receptor Target Genes DKK1 and SFRP1 Does Not Potentiate the Hair Growth-Promoting Effect of SFRP1 Silencing in Healthy Human Hair Follicles Ex VivoInternational Journal of Molecular SciencesFebruary 13, 2026David Broadley, Alizée Le Riche, Ying Yu, Helene El-Bacha, Hanieh Erdmann, Francisco Jimenez, Mikhail Geyfman, Neil Poloso, Janin Edelkamp, Marta Bertolinidiluted in a 1:1 ratio in culture medium and absorbances were measured at 450 nm on a plate reader (GloMax® Discover System, Promega, Madison, WI, USA). The concentrations were determined based on internal standards. Standard

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