Bio-Dot SF Apparatus from Bio-Rad

Product Specs
ItemBio-Dot SF Apparatus
CompanyBio-Rad
Price $944.00Get Pricing
Catalog Number1706542
Quantity1EA

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Pervasive suppressors halt the spread of selfish Segregation Distorter in a natural populationGENETICSAugust 5, 2026Ching-Ho Chang, Tyler Handler, Nicholas J Fuda, Danielle Pascua, Taylar Mouton, Amanda M Larracuente0.45 μm Nylon membrane [Thermo Scientific 77016] in triplicate using Bio-Dot SF slot blotter [Bio-Rad #1706542], and each slot was rinsed with 150 μL loading buffer and 200 μL 400 mM Tris-Cl (pH 7.5). After rinsing

(2) Chronic Sleep Disturbance Enhances Inflammation and Collagen Production in Neural- and Myofascial Tissues in Uninjured RatsInt J Mol SciJuly 8, 2026Mikhail A. Kolpakov, Betsy A. Kalicharan, Lewis Bright-Rowe, Frank L. Chen, Khyleisha A. Caesar, Yasmine B. Dahleh, Abby Kegg, Brendan A. Hilliard, Soul M. Moreno, Megan Van Der Bas, et al.slot blot or Western blot methods. A slot blot microfiltration device was used (Bio-Dot® SF Apparatus, 1706542, Biorad, Hercules, CA, USA), per manufacturer’s directions and described methods [103]. After transfer,

(3) Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disordersCellJuly 1, 2026Zinan Zinan Zhou, Lovelace J. Luquette, Guanlan Dong, Junho Kim, Jayoung Ku, Kisong Kim, Nandini Ramesh, Mingyun Bae, Ann Caplin, Diane D. Shao, et al.nitrocellulose membrane (Bio-Rad 1620115) using the Bio-Dot® SF Microfiltration Apparatus (Bio-Rad 1706542) according to the manufacturer’s instructions. Membranes were UV-crosslinked for 10 min and blocked

(4) Therapeutic nanoliposome vaccine targeting multiple Aβ and tau epitopes reduces AD-like brain pathologies and rescues cognitive deficits in 3xTg-AD miceBrain, Behavior, & Immunity - HealthFebruary 1, 2026Chun-Ling Dai, Yiting Song, Yonghua Chen, Yunn Chyn Tung, Wei-Chiao Huang, Cheng-Xin Gong, Jonathan F. Lovellintegrity of SNAP-AD5 in particle form with liposome, we set up a 48-well slot apparatus (Bio-Rad, catalog# 1706542) with the provided instruction. Initially, 50 μL of PBS was applied to each well to check for leakage.

(5) Pre- and postsynaptic upregulation of FasII synergistically underlies neuropathological and behavioral phenotypes in a Drosophila model of myotonic dystrophyNature CommunicationsDecember 18, 2025Alex Chun Koon, Ka Yee Winnie Yeung, Yitao Wu, Lok I Leong, John Tsun Po Cheung, Zhefan Stephen Chen, Shaohong Isaac Peng, Noah S. Armstrong, C. Andrew Frank, Paul Magneron, et al.and equilibrated in 1× PBS (Gibco, 20012-019) and then placed in the Bio-Dot® SF Apparatus (Bio-Rad, 1706542). Membrane slots were rinsed with 100 µL 1× PBS, and then loaded with 20 µg frontal cortex protein in

(6) Differential effects of mercury compounds on mutagenicity, genotoxicity and repair of UV-DNA damageToxicologyDecember 1, 2025Anna Cyran, Giorgiana Madalina Ursu, Casey Krawic, Anatoly ZhitkovichDNA (300 ng) was deposited on 0.22 μm nitrocellulose membrane (1620112) using Bio-Dot SF apparatus (1706542) from Bio-Rad (Hercules, CA, USA). CPDs (CAC-NM-DND-001) and 6–4PPs (CAC-NM-DND-002) were detected with

(7) Creatine Kinase Blockade Disrupts Energy Metabolism and Redox Homeostasis to Suppress Osteosarcoma ProgressionInternational journal of molecular sciencesNovember 28, 2025Kishi S, Sasaki R, Fujiwara-Tani R, Ohmori H, Luo Y, Fujii K, Sasaki T, Goto K, Miyagawa Y, Kawahara I, et al.lysates (1 μg/10 µL TBSB) were applied to nitrocellulose using a Bio-Dot SF Microfiltration Apparatus (#1706542, Bio-Rad, Tokyo, Japan), dried, and processed similarly to Western blots. 4.13. Knockdown Assay CKB

(8) Pervasive suppressors halt the spread of selfish Segregation Distorter in a natural populationbioRxivOctober 17, 2025Ching-Ho Chang, Tyler Handler, Nick Fuda, Danielle Pascua, Taylar Mouton, Amanda M Larracuente0.45um Nylon membrane [Thermo Scientific 77016] in triplicate using Bio-Dot SF slot blotter [Bio-Rad #1706542], and each slot was rinsed with 150ul loading buffer and 200ul 400mM Tris-Cl (pH 7.5). After rinsing

(9) Oxidative damage within alternative DNA structures results in aberrant mutagenic processingNucleic Acids ResFebruary 14, 2025Maha Zewail-Foote, Imee M A Del Mundo, Alex W Klattenhoff, Karen M Vasquez-slot blot assay was carried out according to the protocol recommended by the supplier (Bio-Rad, Catalog #1706542). After transferring the purified DNA fragments, the nitrocellulose membrane was baked for 2 h at 80°C

(10) Dual inhibition of aminoacyl-tRNA synthetase interacting multifunctional protein-2 and α-synuclein by steroid derivative is neuroprotective in Parkinson''s modeliScienceNovember 15, 2024Shin JY, Ha MW, Kim JH, Cheon J, Lee GH, Paek SM, Lee Y.sheets of 3M paper and one sheet of membrane are placed on the dot blot machine (BIO-Dot BIO-RAD, cat#1706542), and the screws were tightened. Samples, 100 μl each, were loaded through vacuum into each well. The

(11) Mouse α-synuclein fibrils are structurally and functionally distinct from human fibrils associated with Lewy body diseasesScience advancesNovember 1, 2024Sokratian A, Zhou Y, Tatli M, Burbidge KJ, Xu E, Viverette E, Donzelli S, Duda AM, Yuan Y, Li H, et al.tris-buffered saline (TBS), using a bio-dot slot format microfiltration apparatus (Bio-Rad, catalog #1706542). Samples were filtered and washed in tris-buffered saline with 0.1% tween-20 detergent (TBST) through

(12) Adjuvanted nanoliposomes displaying six hemagglutinins and neuraminidases as an influenza virus vaccineCell Reports MedicineMarch 19, 2024Zachary R. Sia, Jayishnu Roy, Wei-Chiao Huang, Yiting Song, Shiqi Zhou, Yuan Luo, Qinzhe Li, Dominic Arpin, Hilliard L. Kutscher, Joaquin Ortega, et al.PA",MB-201-0200 Nitrocellulose Membrane,Thermo Fisher Scientific,77012 48-Well Slot Blot Apparatus,Bio-Rad,1706542 "Cell Strainer, 70 μm mesh","VWR, North American",76327–100

(13) Upregulation of LRRK2 following traumatic brain injury does not directly phosphorylate Thr175 tauFrontiers in Cellular NeuroscienceNovember 8, 2023Neil Donison, Matthew Hintermayer, Maegha Subramaniam, Erin Santandrea, Kathryn Volkening, Michael J. Strongnitrocellulose membrane was pre-soaked in TBS and placed in the Bio-Dot SF Microfiltration Apparatus (Bio-Rad #1706542). The membrane was rehydrated by adding TBS to each well, followed by adding equal amounts of protein

(14) Preparation of chaperone-loaded neural stem cell-derived extracellular vesicles to reduce protein aggregation in Huntington’s disease cellular modelsSTAR ProtocolsMarch 1, 2023Bhagyashree S. Joshi, Inge S. Zuhorn-80°C for later use.100.Setting up of FTA Bio-Dot apparatus –a.Open the Bio-Dot SF apparatus (Biorad, 1706542). Make sure it is thoroughly cleaned with water and soap.b.Prepare FTA wash buffer (10 mM Tris-Cl pH

(15) Targeting G-quadruplex for rescuing impaired chondrogenesis in WRN-deficient stem cellsCell & bioscienceDecember 31, 2022Leung AO, Yiu TC, Liu L, Tam HY, Gu S, Tu J, Pei D, Cheung HH.um, Thermo) and filter papers were pre-wet for 10 min in TBS. After assembly of Slot blot apparatus (1706542, Bio-Rad), 200 µL TBS were applied to each well and vacuumed through. 200 µL of samples or TBS were

(16) NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitorsScience advancesDecember 9, 2022Meroni A, Grosser J, Agashe S, Ramakrishnan N, Jackson J, Verma P, Baranello L, Vindigni A.was then deposited onto a 0.45-μm nitrocellulose membrane (Amersham) using a slot blotting apparatus (1706542, Bio-Rad). TOP1-ccs were detected by overnight incubation with primary rabbit anti-TOP1 antibody (1:2000;

(17) Loss of heat shock factor initiates intracellular lipid surveillance by actin destabilizationCell ReportsOctober 18, 2022Abigail Watterson, Sonja L.B. Arneaud, Naureen Wajahat, Jordan M. Wall, Lexus Tatge, Shaghayegh T. Beheshti, Melina Mihelakis, Nicholas Y. Cheatwood, Jacob McClendon, Atossa Ghorashi, et al.added to samples for a final concentration of 0.5% SDS. The Bio-Dot SF slot blot apparatus (Bio-Rad 1706542) was assembled with a 0.45-μm pore size cellulose acetate membrane (Sterlitech CA0453001) atop pre-soaked

(18) Filter trapping protocol to detect aggregated proteins in human cell linesSTAR protocolsSeptember 16, 2022Chhipi-Shrestha JK, Yoshida M, Iwasaki S.Paper,Bio-Rad,Cat#1620161 0.2-μm cellulose acetate membrane filter,Cytiva,Cat#10404180 Bio-Dot SF Apparatus,Bio-Rad,Cat#1706542 Square petri dish,Greiner Bio-One,Cat#07-000-330 Seesaw shaker,Biocraft,BC-700 Odyssey CLx Infrared Imaging System

(19) XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repairNature communicationsAugust 13, 2022Blessing C, Apelt K, van den Heuvel D, Gonzalez-Leal C, Rother MB, van der Woude M, González-Prieto R, Yifrach A, Parnas A, Shah RG, et al.vacuum-transferred to a nitrocellulose membrane using the Bio-Dot or Bio-Dot-SF apparatus (Bio-Rad, 1706542/5). Membranes were baked at 80 oC in a Bio-Rad’s Gel Dryer model 583, blocked in 5% milk in PBS with

(20) A rapid method to visualize human mitochondrial DNA replication through rotary shadowing and transmission electron microscopyNucleic Acids ResearchDecember 2, 2021Martin Kosar, Daniele Piccini, Marco Foiani, Michele Giannattasio#GERPN203B), previously equilibrated with TE, using a Bio-Dot SF microfiltration apparatus (Bio-Rad, #1706542) following manufacturer instructions. The Hybond N + membrane was washed with 200 μl of TE and exposed

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