EpiMark 5-hmC and 5-mC Analysis Kit from New England Biolabs

EpiMark 5-hmC and 5-mC Analysis Kit - 20 rxns

5-methylcytosine (5-mC) is the predominant epigenetic mark in mammalian genomic DNA. 5-hydroxymethylcytosine (5-hmC) is a newly discovered epigenetic modification that is presumably generated by oxidation of 5-mC by the TET family of cytosine oxygenases. Techniques exist that can identify 5-mC in genomic DNA, but the most commonly used method, bisulfite sequencing, is laborious and cannot distinguish between 5-mC from 5-hmC. The EpiMark™ 5-hmC and 5-mC Analysis Kit can be used to analyze and quantitate 5-methylcytosine and 5-hydroxymethylcytosine within a specific locus. The kit distinguishes 5-mC from 5-hmC by the addition of glucose to the hydroxyl group of 5-hmC via an enzymatic reaction utilizing T4 beta-glucosyltransferase (T4-BGT). When 5-hmC occurs in the context of CCGG, this modification converts a cleavable MspI site to a noncleavable one. The EpiMark 5-hmC and 5-mC Analysis Kit has the following features:
* Complete conversion of 5-hmC to glucosylated 5-hmC in DNA.
* Discrimination between 5-mC and 5-hmC in CCGG sequences using enzymatic.
*digestion and PCR amplification.
* Relative quantitation of 5-mC and 5-hmC.
* Easy-to-use protocol.