ExPERT GTx® Electroporation System from MaxCyte

Product Specs
ItemExPERT GTx® Electroporation System
CompanyMaxCyte
Price Get Pricing Get Quote for this Product
Catalog NumberE-GTx
Dimensions(WxDxH) 8.7 x 17.6 x 19.4 in. (221 x 448 x 493 mm)
QuantityEA
ApplicationsCell Therapy, Gene Editing, Antibody & Protein Production
MethodStatic and Flow Electroporation®
Volume15 µL to 100 mL
Flow Rate8 mL / minute
Throughput1x10⁵-2x10¹⁰ cells per sample
Volume Range20 µL to 100 mL

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MaxCyte

9713 Key West Avenue

Suite 400

Rockville, MD 20850

United States

Phone: +1-301-944-1700

Company Profile

Website: maxcyte.com

(1) An integrated, scaled approach to resolve TSC2 variants of uncertain significancebioRxivJanuary 22, 2026Carina G Biar, Ziyu R Wang, Nathan D. Camp, Daniel L Holmes, Melinda K Wheelock, Sriram Pendyala, Abby V McGee, Pankhuri Gupta, Abbye E McEwen, Malvika Tejura, et al.transferred to a Maxcyte assembly and transfected with the Expanded T Cell-1 protocol on a Maxcyte ExPERT GTx electroporator. Cell staining and sorting Following transfection, cells were cultured for 6 days with

(2) c-JUN enhances CRISPR knockin anti-B7-H3 CAR T cell function in small cell lung cancer and thoracic SMARCA4-deficient undifferentiated tumorsCell Reports MedicineJanuary 20, 2026Hyatt Balke-Want, Vimal Keerthi, Maria Del Carmen Arenas, Yiyun Chen, Meena Malipatlolla, Dorota D. Klysz, Peng Xu, Katie Ho, Kyle Asano, David Stahl, et al.al.64,https://doi.org/10.1186/s13059-014-0550-8 ,, Other,Other,Other ,, Incucyte ZOOM,Sartorius,N/A Beckman CytoFLEX analyzer,Beckman Coulter,N/A ExPERT GTx™ GMP Electroporator,MaxCyte,N/A OC-100 × 2,MaxCyte,N/A CL1.1 RUO,MaxCyte,N/A Cellometer Auto 2000,Nexcelom,N/A G-Rex®6

(3) Efficient and Traceless Aptamer‐Based Serial Selection of Naïve and Early Memory CD8 T Cells for CAR T Cell TherapyAdvanced Healthcare MaterialsJanuary 1, 2026Abe Y. Wu, Emmeline L. Cheng, Nataly Kacherovsky, Abigail Marking, Arie Lin‐Goldstein, Clinton M. Heinze, Stephen J. Salipante, Michael C. Jensen, Suzie H. Punloaded in an R-50×3 processing assembly (MaxCyte) and electroporated using the Jurkat protocol on the ExPERT GTx system (MaxCyte). Electroporated cells were stained with 50 nM CD62L antibody (clone DREG-56) or CD62LApt.88

(4) Targeting oncogenic fusion-driven NUT carcinoma with CRISPR-Cas9 genome editingMolecular Therapy OncologyDecember 18, 2025Maxim F. Carle, Tahereh Mohammadian Gol, Justin S. Antony, Alicia Roig-Merino, Mary E. Carter, Ulrich M. Lauer, Markus Mezger, Linus D. Klokertransfection of the Cas9 RNP, we first determined appropriate electroporation settings using the MaxCyte ExPERT GTx electroporator. Four NC cell lines and A549 non-small cell lung cancer (NSCLC) control cells were electroporated

(5) Cost-effective strategies for CAR-T cell therapy manufacturingMol Ther OncolJune 18, 2025Luiza Abdo, Leonardo Ribeiro Batista-Silva, Martín Hernán Bonaminousing electroporation. Electroporation of T cells or PBMC using 4D-Nucleofector System (Lonza),31,40 ExPERT GTx (MaxCyte),41 Neon Transfection System (Thermo-Fisher),33 and CliniMACS Prodigy (Miltenyi Biotec)24 is

(6) BCL11A-deficient human erythropoiesis is impaired in vitro and after xenotransplantation into mice∗Blood AdvancesJune 10, 2025Yoonjeong Jang, Ruopeng Feng, Lance E. Palmer, Thiyagaraj Mayuranathan, Yu Yao, Kalin Mayberry, Sheng Zhou, Jian Xu, Jeffrey Michael Gossett, Guolian Kang, et al.further. Methods Genome editing of CD34+ HSPCs using Cas9 RNP Genome editing was performed using ExPERT GTx (MaxCyte, Inc, Rockville, MD) with electroporation program HSC3 or 4D-Nucelofector (Lonza, Basel, Switzerland)

(7) All-in-one CRISPR/Cas-engineered glucocorticoid-receptor knock-out EBV-gp350-CAR knock-in T cells are potent and resistant to dexamethasoneExp Hematol OncolMarch 19, 2025Theresa Kaeuferle, Maximilian Zwermann, Nadine Stoll, Paulina Ferrada-Ernst, Lena Jablonowski, Reinhard Zeidler, Semjon Willier, Dana Stenger, Abdallah Yassin, Renata Stripecke, et al.GenScript Biotech Corporation, Piscataway, New, Jersey, USA) was added. Cells were electroporated on the ExPERT GTx in Electroporation Buffer EP (both MaxCyte Inc., Rockville, Maryland, USA) via Expanded T-cell 4. After

(8) Generation and GMP scale-up of human CAR-T cells using non-viral Sleeping Beauty transposons for B cell malignancesMolecular Therapy Methods & Clinical DevelopmentMarch 13, 2025Begoña Diez, Cristina Calviño, María Fernández-García, Paula Rodríguez-Márquez, Saray Rodríguez-Díaz, Rebeca Martínez-Turillas, Candela Ceballos, Jorge Illarramendi, Juana Serrano-López, Csaba Miskey, et al.activated T cells were electroporated using the optimized conditions (0.5:0.5 μg of MC:mRNA) in the MaxCyte ExPERT GTx electroporation system and were expanded for 11 days in G-Rex bioreactors, refeeding the cultures with fresh cytokines on day

(9) Magnetic bead-sensitized optoporation coupled with antibodies-based activation for mRNA CAR-T cell manufacturingMolecular Therapy Methods & Clinical DevelopmentMarch 13, 2025Noelia Maldonado-Pérez, Marie-Agnès Doucey, Dzhangar Dzhumashev, Darel Martínez Bedoya, Luis Castillo Cantero, Caroline Boudousquie, Yann Pierson, Luc Henry, Valérie Dutoit, Denis MiglioriniElectroporation buffer (Cytiva Life Sciences). We electroporated 2 × cells T cells with 5 μg of mRNA using ExPERT GTx (MaxCyte). Following electroporation, T cells were incubated overnight at 37°C with 5% CO2 in complete

(10) Enhanced fetal hemoglobin production via dual-beneficial mutation editing of the HBG promoter in hematopoietic stem and progenitor cells for β-hemoglobinopathiesStem Cell Research & TherapyDecember 31, 2024Prathibha Babu Chandraprabha, Manoj Kumar K. Azhagiri, Vigneshwaran Venkatesan, Wendy Magis, Kirti Prasad, Sevanthy Suresh, Aswin Anand Pai, Srujan Marepally, Alok Srivastava, Kumarasamypet Murugesan Mohankumar, et al.processing assembly (OC25 × 3 or OC100 × 2) for electroporation with pulse code HSC-5 in the MaxCyte® ExPERT® GTx™ unit. The processing unit was immediately placed directly in a 37 °C CO2 incubator and plated in culture

(11) Preclinical Evaluation of AZD6422, an Armored Chimeric Antigen Receptor T Cell Targeting CLDN18.2 in Gastric, Pancreatic, and Esophageal CancersClinical Cancer ResearchDecember 2, 2024Allison M. Barrett, Zachary T. Britton, Rosa A. Carrasco, Shannon Breen, Maria A.S. Broggi, Amanda L. Hatke, Benjamin Clark, Chunning Yang, Sandrina Phipps, Lorenzo Ortiz, et al.and precomplexed ribonucleoproteins were electroporated in an RUO OC-25x3 cassette (MaxCyte) using an ExPERT GTx electroporation instrument (MaxCyte). PaTu8988 KO cells underwent three rounds of this protocol. Lentivirus

(12) Production of therapeutic levels of human FIX-R338L by engineered B cells using GMP-compatible mediumMolecular Therapy - Methods & Clinical DevelopmentDecember 14, 2023Marion David, Davide Monteferrario, Gaëlle Saviane, Caroline Jeanneau, Irène Marchetti, Coralie F. Dupont, Céline Dumont, Jason D. Fontenot, Maurus de la Rosa, David FenardGaithersburg, MD). ZFN mRNA transcripts were then mixed with B cells at 90 μg/mL and electroporated with ExPERT GTx electroporator according to manufacturer’s recommendations (Maxcyte). Next, cells were recovered, suspended

(13) Optimization of universal allogeneic CAR-T cells combining CRISPR and transposon-based technologies for treatment of acute myeloid leukemiaFrontiers in ImmunologySeptember 19, 2023Cristina Calviño, Candela Ceballos, Ana Alfonso, Patricia Jauregui, Maria E. Calleja-Cervantes, Patxi San Martin-Uriz, Paula Rodriguez-Marquez, Angel Martin-Mallo, Elena Iglesias, Gloria Abizanda, et al.mRNA, 3µM of each sgRNA (targeting TRAC and B2M), and 1,5µM Cas9 previously mixed as an RNP, using the ExPERT GTx™ electroporation device from MaxCyte, according to manufacturer’s instructions. CAR-T cells were expanded

(14) Non-viral chimeric antigen receptor (CAR) T cells going viralImmuno-Oncology and TechnologyJune 1, 2023H. Balke-Want, V. Keerthi, A. Cadinanos-Garai, C. Fowler, N. Gkitsas, A.K. Brown, R. Tunuguntla, M. Abou-el-Enein, S.A. Feldmanpatients with B-cell leukemia and lymphoma by using electroporation with various devices, including the ExPERT GTx (MaxCyte Inc., Gaithersburg, MD), 4D-Nuclefector LV Unit (Lonza, Basel, Switzerland), CliniMACS Electroporator

(15) Non-viral TRAC-knocked-in CD19(KI)CAR-T and gp350(KI)CAR-T cells tested against Burkitt lymphomas with type 1 or 2 EBV infection: In vivo cellular dynamics and potencyFrontiers in immunologyJanuary 1, 2023Braun T, Pruene A, Darguzyte M, Vom Stein AF, Nguyen PH, Wagner DL, Kath J, Roig-Merino A, Heuser M, Riehm LL, et al.double-stranded end modifications achieve >40% CAR KI rates at the clinical scale with the MaxCyte ExPERT GTx® device (33). In addition, the design of the HDRTs and inclusion of tags can be optimized to reduce

(16) Challenges in Gene Therapy for Somatic Reverted Mosaicism in X-Linked Combined Immunodeficiency by CRISPR/Cas9 and Prime EditingGenes (Basel)December 13, 2022Yujuan Hou, Guillermo Ureña-Bailén, Tahereh Mohammadian Gol, Paul Gerhard Gratz, Hans Peter Gratz, Alicia Roig-Merino, Justin S. Antony, Andrés Lamsfus-Calle, Alberto Daniel-Moreno, Rupert Handgretinger, et al.complex at 6 µM. Subsequently, 2.5 × 106 K–562 cells and 1.5 × 106 T cells were electroporated with ExPERT GTx® using R-50 × 3 processing assemblies with the “K562” and “Expanded T cell 3” programs (MaxCyte®, Rockville,

(17) A nonviral, nonintegrating DNA nanovector platform for the safe, rapid, and persistent manufacture of recombinant T cellsScience AdvancesApril 16, 2021Matthias Bozza, Alice De Roia, Margareta P. Correia, Aileen Berger, Alexandra Tuch, Andreas Schmidt, Inka Zörnig, Dirk Jäger, Patrick Schmidt, Richard P. Harbottleevaluated using two different large-scale electroporation devices: the Lonza LV Unit and the MaxCyte ExPERT GTx platform, with 1 × 108 and 3 × 108 CD3+ cells, respectively. With both devices, in comparison to the

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