Neon Transfection System Pipette from Thermo Fisher Scientific

Product Specs
ItemNeon Transfection System Pipette
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog NumberMPP100
QuantityEA
MethodElectroporation (Pipette-based)
Cell TypeMany cell types, including difficult-to-transfect cells, primary, and stem cells

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Intrinsic tumor cell line immunogenicity may drive CAR-independent T cell responses and confound CAR T cell preclinical modelingJ Immunother CancerJuly 23, 2026Michael K Sheng, Wahed A Firoz, Kelly Loi, Logan V Vick, Craig P Collins, Ryan N Nielsen, Jessica L Schmeling, Shuchi Gulati, Sean J Judge, Robert J Canter, et al.temperature. Afterwards, 50 µL of each RNP mixture was added to 100 µL of CAR T cells, collected in a 100 µL Neon transfection system pipette and electroporated using a Neon Electroporation System set to the conditions 1600 V/10 ms/3 pulses.

(2) Host microRNA-31-5p regulates KHDRBS3 expression and modulates viral gene expression during KSHV lytic reactivationCell ReportsApril 1, 2026Soo Mi Lee, Christopher L. Avalos, Christos Miliotis, Hanna M. Doh, Erica Chan, Kenneth M. Kaye, Frank J. Slack,Thermo Fisher Scientific,Cat#MPK10025 Neon Pipette Station,Thermo Fisher Scientific,Cat#MPS100 Neon Pipette,Thermo Fisher Scientific,Cat#MPP100 RIPA Buffer,Boston BioProducts,Cat#BP-115 Phosphatase Inhibitor Cocktail 2,Sigma-Aldrich,Cat#P5726 Phosphatase Inhibitor Cocktail 3

(3) Splice‑site variant c.3531+1G>T in COL1A1 in a family with osteogenesis imperfectaMolecular Medicine ReportsMay 28, 2025Yanru Huang, Yixi Zhou, Lutan Zhang, Ye Shen, Xingmei Yao, Jieqiong Xie, Libin Mei, Yunsheng Ge(Neon™ Transfection System; cat. no. MPK5000; Thermo Fisher Scientific, Inc.), Neon Transfection System Pipette (cat. no. MPP100; Thermo Fisher Scientific, Inc.) and Neon Transfection System 100 µl Kit (cat. no. MPK10096; Thermo Fisher Scientific

(4) Mice deficient in TWIK-1 are more susceptible to kainic acid-induced seizuresiScienceJanuary 17, 2025Ajung Kim, Yeonju Bae, Changdev G. Gadhe, Hyun-Gug Jung, Esther Yang, Hyun Kim, Jaekwang Lee, Chanseob Shim, Young Hoon Sung, Junyeol Noh, et al.Duolink® In Situ PLA® Probe Anti-Mouse MINUS,Sigma-Aldrich,DUO92004 Duolink® In Situ Detection Reagents Green,Sigma-Aldrich,DUO92014 Neon® Transfection System,Thermo Fisher,"MPP100, MPS100, MPK1025" ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models

(5) Optimized Prime Editing of Human Induced Pluripotent Stem Cells to Efficiently Generate Isogenic Models of Mendelian DiseasesInternational Journal of Molecular SciencesDecember 26, 2024Rodrigo Cerna-Chavez, Alba Ortega-Gasco, Hafiz Muhammad Azhar Baig, Nathan Ehrenreich, Thibaud Metais, Michael J. Scandura, Kinga Bujakowska, Eric A. Pierce, Marcela Garita-HernandezNeon™ Transfection System Pipette Station (MPS100), a Neon™ Transfection System Pipette (Invitrogen MPP100), and a Neon™ Transfection System 10 μL Kit (Invitrogen MPK1096). hiPSCs at 90% confluence were dissociated

(6) Immunoprecipitation of Reporter Nascent Chains from Active Ribosomes to Study Translation EfficiencyBio ProtocSeptember 20, 2023Roberta Cacioppo, Catherine Lindoncatalog number: MPK5000)Neon Transfection System Pipette (Thermo Fisher Scientific, catalog number: MPP100)Micro-volume spectrophotometer NanoDropTM Lite (Thermo Fisher Scientific)Tube rotator (Fisherbrand,

(7) Immunoprecipitation of Reporter Nascent Chains from Active Ribosomes to Study Translation EfficiencyBIO-PROTOCOLSeptember 20, 2023Roberta Cacioppo, Catherine Lindoncatalog number: MPK5000)Neon Transfection System Pipette (Thermo Fisher Scientific, catalog number: MPP100)Micro-volume spectrophotometer NanoDropTM Lite (Thermo Fisher Scientific)Tube rotator (Fisherbrand,

(8) ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterningDevelopment (Cambridge, England)July 1, 2022D'Amico G, Fernandez I, Gómez-Escudero J, Kim H, Maniati E, Azman MS, Mardakheh FK, Serrels B, Serrels A, Parsons M, et al.mixed with CRISPR/Cas9-EGFP plasmid DNA (10 μg) and loaded into a 100 μl Neon electroporation tip (Mpp100, Invitrogen). Electroporations were performed using 1300 mV for 20 ms with the two-pulse programme on

(9) YB1 Is a Major Contributor to Health Disparities in Triple Negative Breast CancerCancers (Basel)December 14, 2021Priyanka Shailendra Rana, Wei Wang, Akram Alkrekshi, Vesna Markovic, Amer Khiyami, Ricky Chan, Adam Perzynski, Natalie Joseph, Khalid Sossey-AlaouiNeon Transfection System. Per electroporation, 2 × 105 cells were taken up in a 10 µL Neon tip using the Neon Transfection System pipette (Invitrogen). The electroporation was performed by applying 4 pulses at 1400 Volts for 10 ms to all

(10) Long non-coding RNA GHET1/miR-105/RAP2B axis regulates the progression of acute myeloid leukemiaJournal of CancerOctober 17, 2020Yue Xiao, Tangfei Li, Qianfu Xue, Lili Miaotaken up in 10 μL resuspension buffer containing 0.8 μg of the plasmid with 10 μL Neon tips using a Neon Transfection System pipette. We used the following parameters for the NB4 and HL-60 cells: pulse voltage (1400 V), pulse width (20

(11) Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative ColitisImmunityApril 16, 2019Castro-Dopico T, Dennison TW, Ferdinand JR, Mathews RJ, Fleming A, Clift D, Stewart BJ, Jing C, Strongili K, Labzin LI, et al.Neon Transfection System,Thermo Fisher Scientific,Cat#MPK5000 Neon Pipette Tip,Thermo Fisher Scientific,Cat#MPP100 OCT embedding medium,Thermo Fisher Scientific,Cat#LAMB/OCT

(12) Comprehensive Protocols for CRISPR/Cas9‐based Gene Editing in Human Pluripotent Stem CellsCurrent Protocols in Stem Cell BiologyAugust 18, 2016David P. Santos, Evangelos Kiskinis, Kevin Eggan, Florian T. MerkleNEON Transfection System,Thermo Fisher Scientific,MPK10096 NEON Transfection System Pipette,Thermo Fisher Scientific,MPP100 "Novex TBE Gel, 4–20%, 10 well",Thermo Fisher Scientific,EC6225BOX "Nylon Membrane, Positively Charged",Sigma-Aldrich,11209299001 One Shot TOP10 Chemically Competent E. coli

(13) Murine Norovirus: Propagation, Quantification, and Genetic ManipulationCurrent Protocols in MicrobiologyMay 1, 2014Seungmin Hwang et al.MPK10096; (contains Buffers E and R, gold tips, and tip holder) NEON pipet (Life Technologies, cat. no. MPP100) 6-well cell culture dishes 15-ml sterile conical tubes (e.g., BD Falcon) Additional reagents and equipment

(14) "The NOGO receptor NgR2, a novel αVβ3 integrin effector, induces neuroendocrine differentiation in prostate cancer"Scientific reportsJanuary 1, 0001"Quaglia F, Krishn SR, Sossey-Alaoui K, Rana PS, Pluskota E, Park PH, Shields CD, Lin S, McCue P, Kossenkov AV, et al."Neon Transfection System. Per electroporation, 2 × 105 cells were taken up in a 10 μl Neon tip using the Neon Transfection System pipette (Invitrogen). The electroporation was performed by applying 3 pulses at 1450 Volts for 10 ms to PC3 cells

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