EM AC20 Automatic contrasting instrument from Leica Microsystems

Product Specs
ItemEM AC20 Automatic contrasting instrument
CompanyLeica Microsystems
Price Get Pricing Get Quote for this Product
Catalog NumberEM AC20
QuantityEach
ApplicationsAutomated staining and contrasting for TEM sample preparation
TypeAutomatic Contrasting Instrument

Specifications/Features

60 runs per one set of Ultrostains
Low reagent consumption
High contrast

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Leica Microsystems

10 Parkway North Blvd., Suite 300

Deerfield, Illinois 60015

United States

Phone: 800 248 0123

Company Profile

Website: www.leica-microsystems.com

(1) Caspase-3/7 deficiency results in enhanced intestinal inflammation and reduced tumorigenesisSci AdvMarch 20, 2026Wei Xie, Laura Wyckaert, Mike Vadi, Bruno Verstraeten, Tatyana Divert, Jef Haerinck, Riet De Rycke, Femke Baeke, Mohamed Lamkanfi, Geert Berx, et al.interference color were cut using an ultramicrotome (Leica EM UC6), followed by a poststaining in a Leica EM AC20 for 40 min in uranyl acetate at 20°C and for 10 min in lead stain at 20°C. Sections were collected on

(2) Base barrier cells provide compartmentalization of choroid plexus, brain and CSFNature NeuroscienceFebruary 12, 2026Daan Verhaege, Clint De Nolf, Lore Van Acker, Wouter Claeys, Jonas Castelein, Elien Van Wonterghem, Griet Van Imschoot, Pieter Dujardin, Ward De Spiegelaere, Esther Hoste, et al.ultra-microtome (Leica EM UC7), followed by post-staining with uranyl acetate and lead citrate in a Leica EM AC20 and collected on formvar-coated copper slot grids. They were viewed with a JEM 1400plus transmission

(3) An integrative ultrastructural and transcriptomic analysis of host–pathogen interactions with human brain microvascular endothelial cells during cryptococcal infection: a preliminary studyFrontiers in Cellular and Infection MicrobiologyFebruary 9, 2026Priya Krishnan, Gautham Arunachal Udupi, Rashmi Santhoshkumar, Manjunatha. M. V., Veena Kumari H. B., Netravathi, Nagarathna S.ultramicrotome (Leica UC7, Germany) ultrathin sections were collected on copper grids and stained using Leica EM AC20 automatic contrasting system (Leica, Germany). Saturated methanolic uranyl acetate (TAAB, UK) and 3%

(4) Mutations in VPS18 lead to a neutrophil maturation defect associated with disturbed vesicle homeostasisCell Death & DiseaseJanuary 12, 2026Jincheng Gao, Almke Bader, Monika I. Linder, Jingyuan Cheng, Mathis Richter, Raul da Costa, Annette Zehrer, Karl Mitt, Bastian Popper, Felix Meissner, et al.Stain (Electron Microscopy Sciences) and 3% lead citrate (Leica) using the contrasting system Leica EM AC20 (Leica). Imaging was carried out using the JEOL-1200 EXII transmission electron microscope (JEOL) at

(5) Axonal structure-function relationships across experimental modalitiesImaging Neurosci (Camb)December 22, 2025Christian S. Skoven, Mariam Andersson, Miren Lur Barquin Torre, Marco Pizzolato, Hartwig R. Siebner, Tim B. DyrbyLeica Microsystems, Wetzlar, Germany). The sections were stained on the grids with uranyl acetate and lead citrate (EM AC20; Leica Microsystems, Wetzlar, Germany). 2.5.2.2 Cryofixation of fresh tissue (“Cryo-TEM”) Four additional

(6) β-catenin condensation facilitates clustering of the cadherin/catenin complex and formation of nascent cell-cell junctionsNature CommunicationsDecember 6, 2025Jooske L. Monster, Caterina Manzato, Jan A. van der Beek, Willem-Jan Pannekoek, Janneke A. Hummelink, Michael A. Hadders, Cecilia de Heus, Judith Klumperman, Jurian Schuijers, Martijn GloerichKnife 45°. Sections were placed on Formvar- and carbon-coated copper grids and poststained in a Leica EM AC20 (Leica Microsystems) using uranyl and lead citrate. Samples were imaged on a Tecnai T20 (FEI Tecnai)

(7) Neprilysin 4 controls acrosome structure and male fertility in Drosophila melanogasterCommunications BiologyNovember 22, 2025Annika Buhr, Maike Spielmeyer, Eva Cordes, Leonhard Breitsprecher, Rico Franzkoch, Isabel Kipp, Olympia Ekaterini Psathaki, Stefan Walter, Achim Paululat, Heiko Meyersections were contrasted with 3% uranyl acetate for 30 min and 2% lead citrate for 20 min in the LEICA EM AC20 (Leica, Wetzlar, Germany) and subsequently analyzed using a TEM 200 keV JEM2100-Plus (JEOL) equipped

(8) A novel amphibian herpesvirus (candidate Batravirus ranidallo5) associated with disease in free-ranging Iberian painted frogs (Discoglossus galganoi) in SpainScientific ReportsNovember 21, 2025Jaime Bosch, Barbora Thumsová, Justina Pérez-Martín, Carlos Caballero-Díaz, Roser Velarde, Albert Martínez-Silvestre, Jasmine Portmann, Francesco C. OriggiSteinheim, Germany) and 3% lead citrate for 5 min at 20˚C in an automatic contrasting system Leica EM AC20. Sections were then examined under a JEM-1400 transmission electron microscope (JEOL, Akishima, Tokyo,

(9) A transmission electron microscopy platform for assessing mitochondrial and nuclear architecture in cardiomyocytesCell Reports MethodsNovember 17, 2025Mara Kiessling, Juergen Gindlhuber, Amalia Sintou, Ingrid Matzer, Snježana Radulović, Viktoria Trummer-Herbst, Andonita Ajdari, Julia Voglhuber-Höller, Michael Holzer, Tristan A. Rodriguez, et al.on Pioloform-coated copper grids and post-stained with uranyl acetate and lead citrate using a Leica EM AC20. While we were able to detect and capture the central nuclear plane containing the perinuclear regions

(10) CARD14 signaling in intestinal epithelial cells induces intestinal inflammation and intestinal transit delayEMBO Molecular MedicineOctober 23, 2025Aigerim Aidarova, Marieke Carels, Mira Haegman, Yasmine Driege, Steven Timmermans, Eline Van Damme, Javier Aguilera-Lizarraga, Maria Francesca Viola, Rita de Cássia Collaço, Joan Manils, et al.interference color were cut using an ultramicrotome (Leica EM UC6), followed by a post-staining in a Leica EM AC20 for 40 min in uranyl acetate at 20 °C and for 10 min in lead stain at 20 °C. Sections were collected

(11) Tubulin Cytoskeleton Organization in Cells of Determinate Nodules in Vigna radiata, Vigna unguiculata, and Lotus corniculatusPlants (Basel)September 26, 2025Anna B. Kitaeva, Pyotr G. Kusakin, Artemii P. Gorshkov, Anna V. Tsyganova, Viktor E. Tsyganovmin and then with lead citrate for 1 min in an automatic contrast system for ultrathin sections of EM AC20 (Leica Microsystems) at 21 °C. The samples were examined and photographed in a transmission electron

(12) Phenotypically distinguishable eosinophilic cells do not impact epithelial functions in a triple-culture in vitro intestinal modelFront ImmunolAugust 4, 2025Christoph Benkstein, Laurin Mosig, Daniel Vondran, Heidi Schlichting, Lea Kissing, Bente Wohlert, Ida Gensmer, Larissa Nogueira de Almeida, Peter König, Kerstin Fibelkorn, et al.blocks were cut into 70–90 nm sections on an Ultracut E (Leica) and the sections got contrasted in an EM AC20 (Leica) with 0.5% uranyl acetate and 1% lead citrate. Pictures were taken on a JEM.1011 (Jeol) transmission

(13) Melatonin and omega-3 neuroprotection in prenatal rat spinal cord exposed to 900 MHz electromagnetic fieldBiomolecules and BiomedicineJuly 21, 2025Ömür Gülsüm Deniz, Gamze Altun, Süleyman KaplanUSA). Thin sections were mounted on copper grids and stained using an automatic contrast system (Leica EM AC20, Leica Microsystems GmbH, Germany) with 0.5% uranyl acetate and 3% lead citrate. Ultrastructural images

(14) Pex3 promotes formation of peroxisome-peroxisome and peroxisome-lipid droplet contact sitesSci RepJuly 8, 2025Lucía Amado, Louis Percifull, Rico Franzkoch, Vico Flatemersch, Eleni Joana Brüggemann, Olympia Ekaterini Psathaki, Maya Schuldiner, Maria Bohnert, Margret H. Bülow, Ayelén González Montorowere then contrasted with 3% uranyl acetate for 30 min and 2% lead citrate for 20 min in the LEICA EM AC20 and subsequently analysed with a TEM at 200 kV (JEM2100Plus, JEOL, Japan) equipped with a 20-megapixel

(15) Elevated NGF provokes decidual lipid peroxidation and promotes preterm birth in miceJ Transl MedApril 28, 2025Yuhang Ge, Ruxin Teng, Zhaoyu Jia, Yongyue Li, Yafang Lu, Jia Yuansaturated aqueous uranyl acetate and lead citrate solution for 30 min in a staining machine (Leica, EM AC20), and subsequently photographed and analyzed using a Thermo Fisher transmission electron microscope

(16) Increased TMEM106B levels lead to lysosomal dysfunction which affects synaptic signaling and neuronal healthMolecular neurodegenerationApril 23, 2025Perneel J, Lastra Osua M, Alidadiani S, Peeters N, De Witte L, Heeman B, Manzella S, De Rycke R, Brooks M, Perkerson RB, et al.interference colour were cut using an ultramicrotome (Leica EM UC6) followed by a post-staining in a Leica EM AC20 for 40 min in uranyl acetate and for 10 min in lead stain at 20 °C. Sections were collected on formvar-coated

(17) Sphingosine-mediated death of Pseudomonas aeruginosa involves degradation of cardiolipin by the maintenance of outer lipid asymmetry systemInfection and ImmunityApril 8, 2025Heike Grassmé, Gregory C. Wilson, Yuqing Wu, Mike Hasenberg, Simone Keitsch, Federico Caicci, Michael J. Edwards, Ildiko Szabo, Erich Gulbinsaqueous uranyl acetate and Reynold’s lead citrate solution using an automated contrasting instrument (EM AC20, Leica Microsystems, Wetzlar, Germany). Image acquisition was performed with a JEM 1400Plus TEM (JEOL

(18) Any1 is a phospholipid scramblase involved in endosome biogenesisJ Cell BiolApril 7, 2025Jieqiong Gao, Rico Franzkoch, Cristian Rocha-Roa, Olympia Ekaterini Psathaki, Michael Hensel, Stefano Vanni, Christian Ungermanngrids were contrasted with 3% uranyl acetate for 30 min and 2% lead citrate for 20 min in the LEICA EM AC20. Subsequently, sections were analyzed using a JEOL TEM JEM2100-Plus at 200 keV equipped with a 20-megapixel

(19) Rab10 regulates neuropeptide release by maintaining Ca2+ homeostasis and protein synthesisElifeApril 2, 2025Jian Dong, Miao Chen, Jan R T van Weering, Ka Wan Li, August B Smit, Ruud F Toonen, Matthijs Verhageon single-slot, formvar-coated copper grids, and stained in uranyl acetate and lead citrate (Leica EM AC20). Sections were imaged in a JEOL1010 transmission electron microscope (JEOL) at 60 kV while being blinded

(20) Investigating Neotenic and Metamorphic Axolotl Brain Complexity: A Stereological and Immunohistochemical PerspectiveJ Comp NeurolMarch 20, 2025Arife Ahsen Kaplan, Gürkan Öztürk, Sadık Bay, İlknur Keskinunderwent contrasting using a 0.05% uranyl acetate and 2% lead citrate solution with a contrasting device (EM AC20, Leica). The second tissue preparation protocol was adapted from Yi̇ği̇t et al. (2023) for the detailed

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Leica EM AC20

EM AC20 Automatic contrasting instrument from Leica Microsystems

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Specifications/Features

Leica Microsystems

Citations (159)

Reviews

EM AC20 Automatic contrasting instrument from Leica Microsystems

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