DTT, Molecular Grade (Dry Powder) from Promega

Product Specs
ItemDTT, Molecular Grade (Dry Powder)
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV3151
Quantity5g

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Small-molecule RNA therapeutics to target prostate cancerCancer CellMay 12, 2025Duygu Kuzuoglu-Ozturk, Hao G. Nguyen, Lingru Xue, Emma Figueredo, Vishvak Subramanyam, Isabelle Liu, Kenya Bonitto, Ashish Noronha, Adrianna Dabrowska, Janet E. Cowan, et al.SIAL-11836170001 4x Laemmli Sample Buffer,Bio-Rad,Cat# 1610747 "DTT, Molecular Grade (Dry Powder)",Promega Corp,Cat# V3151 NP-40 Surfact-Amps Detergent Solution,Thermo Fisher,Cat# PI28324 "Sodium deoxycholate, 10%",Thomas

(2) Applying LFQRatio Normalization in Quantitative Proteomic Analysis of Microbial Co-culture SystemsBIO-PROTOCOLMay 5, 2025Mengxun Shi, Caroline A. Evans, Josie L. McQuillan, Josselin Noirel, Jagroop Pandhal(Sigma-Aldrich, catalog number: 06203/258148) 8. DL-Dithiothreitol (DTT) (Promega, catalog number: V3151) 9. Water, HPLC grade (Fisher, catalog number: W/0106/PB17) 10. Iodoacetamide (IAA) (Sigma-Aldrich,

(3) Unique N-glycosylation signatures in human iPSC derived microglia activated by Aβ oligomer and lipopolysaccharideSci RepApril 10, 2025Xinyu Tang, Ryan Lee Schindler, Jacopo Di Lucente, Armin Oloumi, Jennyfer Tena, Danielle Harvey, Carlito B. Lebrilla, Angela M. Zivkovic, Lee-Way Jin, Izumi Maezawaresuspended in 200 µL of 100 mM NH4HCO3 (MilliporeSigma, Cat# 09830), 5 mM Dithiothreitol (Promega, Cat# V3151) in water to assist in protein denaturing. After gentle homogenization by pipette mixing, samples were

(4) Integration of RNAseq transcriptomics and N-glycomics reveal biosynthetic pathways and predict structure-specific N-glycan expression†Chem SciApril 4, 2025Michael Russelle S. Alvarez, Xavier A. Holmes, Armin Oloumi, Sheryl Joyce Grijaldo-Alvarez, Ryan Schindler, Qingwen Zhou, Anirudh Yadlapati, Atit Silsirivanit, Carlito B. Lebrillaand ammonium bicarbonate (NH4HCO3, cat.# A6141) were from Sigma-Aldrich. Dithiothreitol (DTT, cat.#V3151) was from Promega. PNGase F (cat.#P0704L) was purchased from New England Biolabs. LC-MS-grade trifluoroacetic

(5) 9-aminominocycline potentiates the efficacy of EIDD-1931 and PF-332 by targeting the papain like protease enzyme of SARS-CoV-2Sci RepFebruary 15, 2025Kabita Pandey, Devin Shane M. Lewis, Kyeongin Heo, Arpan Acharya, Travis Fields, Kritika Gowda, George Dean, Srujana Rayalam, Siddappa N. Byrareddy, Vicky Mody, et al.for DUB activity) were purchased from BPS Biosciences (San Diego, CA, USA). Dithiothreitol (DTT, cat#V3151) was obtained from Promega (Madison, WI, USA), and GRL-0617 (cat#1093070-16-6) was from MedChemExpress

(6) Evaluation of the Protective Efficacy of Different Doses of a Chlamydia abortus Subcellular Vaccine in a Pregnant Sheep Challenge Model for Ovine Enzootic AbortionAnimals (Basel)October 17, 2024Morag Livingstone, Kevin Aitchison, Javier Palarea-Albaladejo, Francesca Chianini, Mara Silvia Rocchi, Sergio Gastón Caspe, Clare Underwood, Allen Flockhart, Nicholas Wheelhouse, Gary Entrican, et al.UK; #61743), followed by 2% sarkosyl containing 10 mM dithiothreitol (DTT; Promega, Southampton, UK; #V3151) and differential centrifugation, as described previously [27,36]. The final insoluble COMC pellet was

(7) Neuroprotective mushroomsNeuroImmune Pharmacology and TherapeuticsJune 25, 2024Mai M. Abdelmoaty, Rana Kadry, R. Lee Mosley, Howard E. Gendelmandimethylammonio] propanesulfonate (CHAPS, Thermo Fisher Scientific, 28300), 100 mM dithiothreitol (DTT, Promega, V3151), 2% Pharmalyte (Sigma-Aldrich, GE17–0456-01), and protease and phosphatase inhibitor cocktail (Thermo

(8) N 6 -methyladenosine (M 6 A) in fetal offspring modifies mitochondrial gene expression following gestational nano-TiO 2 inhalation exposureNanotoxicologyJanuary 18, 2024Amina Kunovac, Quincy A. Hathaway, Dharendra Thapa, Andrya J. Durr, Andrew D. Taylor, Saira Rizwan, Daud Sharif, Stephen J. Valentine, John M. Hollanderfor the rest of the sample preparation procedure. 1.2 μL of DTT reducing agent (100 mM DTT (item no. V3151, Promega) in 8 M urea and 100 mM ammonium bicarbonate – pH 8.2) was added to each sample followed by

(9) High-quality nuclei isolation from postmortem human heart muscle tissues for single-cell studiesJournal of Molecular and Cellular CardiologyJune 1, 2023Sarah Araten, Ronald Mathieu, Anushka Jetly, Hoon Shin, Nazia Hilal, Bo Zhang, Katherine Morillo, Deepa Nandan, Indu Sivankutty, Ming Hui Chen, et al.(Sigma-Aldrich 63052–100ML), 2 mM EDTA (Invitrogen REF AM9260G), 0.5 mM EGTA, and 1 mM DTT (Promega REF V3151). Resuspended tissue was transferred to a 5 ml Eppendorf tube (Fisher Catalog No. 14–282-301) and homogenized

(10) The impact of SWI/SNF and NuRD inactivation on gene expression is tightly coupled with levels of RNA polymerase II occupancy at promotersGenome ResearchMarch 16, 2023Sachin Pundhir, Jinyu Su, Marta Tapia, Anne Meldgaard Hansen, James Seymour Haile, Klaus Hansen, Bo Torben Porse4sU-labeled nascent RNA was released from biotin-streptavidin beads by elution in 100 mM DTT (Promega V3151) followed by purification with a NucleoSpin RNA kit (Macherey-Nagel 740955.250) according to the manufacturer's

(11) High-Quality Nuclei Isolation from Postmortem Human Heart Muscle Tissues for Single-Cell StudiesbioRxiv : the preprint server for biologyFebruary 5, 2023Araten S, Mathieu R, Jetly A, Shin H, Hilal N, Zhang B, Morillo K, Nandan D, Sivankutty I, Chen MH, et al.(Sigma-Aldrich 63052–100ML), 2 mM EDTA (Invitrogen REF AM9260G), 0.5 mM EGTA, and 1 mM DTT (Promega REF V3151). Resuspended tissue was transferred to a 5 ml Eppendorf tube (Fisher Catalog No. 14-282-301) and homogenized

(12) Hedgehog acyltransferase catalyzes a random sequential reaction and utilizes multiple fatty acyl-CoA substratesThe Journal of biological chemistryOctober 1, 2022Schonbrun AR, Resh MD.was purchased from Fisher Scientific (catalog # BP300–100). DTT was purchased from Promega (catalog # V3151). Anti-FLAG M2 Affinity Gel (catalog # A2220), and 3× FLAG peptide (catalog # F4799) were purchased

(13) Histone Acid Extraction and High Throughput Mass Spectrometry to Profile Histone Modifications in Arabidopsis thalianaCurrent ProtocolsAugust 24, 2022Ray Scheid, James A. Dowell, Dean Sanders, Jianjun Jiang, John M. Denu, Xuehua ZhongBP300–100) Nonidet P-40 substitute (Thermo Fisher Scientific, 19626) Dithiothreitol (DTT; Promega, V3151), 4 °C Spermine (Acros Organics, 132750010), 4 °C Spermidine (Acros Organics, 132740010), 4 °C

(14) A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic TissuesJournal of Visualized ExperimentsMay 23, 2022Dylan Nicholas Tabang, Danqing Wang, Lingjun Liprotease inhibitor cocktail tablets,Sigma,11697498001, "DTT, Molecular Grade (DL-Dithiothreitol)",Promega,V3151,Protein reducing agent "Ethanol, 200 proof (100%), USP",Fisher,22-032-601, Fisherbrand Analog Vortex

(15) Systems-level effects of allosteric perturbations to a model molecular switchNatureNovember 4, 2021Tina Perica et al.(Guanosine 5′-triphosphate sodium salt hydrate, CAT #G8877, Sigma-Aldrich), 1 mM Dithiothreitol (Promega V3151), 0.1% IGEPAL CA-630 (CAT # I8896, Sigma-Aldrich), and 10% glycerol). Each pellet of approximately 500

(16) Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clonesPhytopathology ResearchDecember 3, 2020Mingmin Zhao, Beatriz García, Araiz Gallo, Ioannis E. Tzanetakis, Carmen Simón-Mateo, Juan Antonio García, Fabio Pasin8000 (PEG-8000) MP Biomedicals 195445 MgCl2 Sigma-Aldrich 442611 Dithiothreitol (DTT) Promega V3151 dNTP mix Thermo Fisher Scientific R1121 β-Nicotinamide adenine dinucleotide (NAD+) New England Biolabs

(17) Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography ChangesJournal of Visualized ExperimentsSeptember 21, 2020Sandeep K. Misra, Joshua S. Sharpamide,Bachem,4000594.001,H-met-NH2.HCl Trypsin,Promega,V5111,Sequencing Grade Modified Trypsin Dithiotheitol (DTT),Promega,V3151,"DTT, Molecular Grade (DL-Dithiothreitol)" Trap C18 column,Thermo Scientific,160454,Thermo Scientific™

(18) Comprehensive structural glycomic characterization of the glycocalyxes of cells and tissuesNature ProtocolsAugust 1, 2020Qiongyu Li, Yixuan Xie, Maurice Wong, Mariana Barboza, Carlito B. Lebrillairrates skin, eyes and respiratory system. Dithiothreitol (DTT, molecular grade, Promega, cat. no. V3151) ! CAUTION DTT causes skin and eye irritation and may cause respiratory irritation. Iodoacetamide

(19) Integrated proteomics and metabolomics analysis reveals differential lipid metabolism in human umbilical vein endothelial cells under high and low shear stressAmerican journal of physiology. Cell physiologyAugust 1, 2019Venturini G, Malagrino PA, Padilha K, Tanaka LY, Laurindo FR, Dariolli R, Carvalho VM, Cardozo KHM, Krieger JE, Pereira ADC.the rate 1:1 (heavy/light). Protein digestion. Proteins (100 ␮g) were reduced using 10 mM DTT (no. V3151; Promega) during 30 min at 37°C and alkylated using 50 mM iodoacetamide (no. I6125; Sigma-Aldrich) 30

(20) Sequencing cell-type-specific transcriptomes with SLAM-ITseqNature ProtocolsAugust 1, 2019Wayo Matsushima, Veronika A. Herzog, Tobias Neumann, Katharina Gapp, Johannes Zuber, Stefan L. Ameres, Eric A. MiskaGlycogen, Molecular Biology Grade (Roche, cat. no. 10899232103) Dithiothreitol (DTT; Promega, cat. no. V3151) CAUTION: DTT is harmful if swallowed and causes skin irritation upon direct contact. Gloves and a lab

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