QX200 ™ Droplet Reader from Bio-Rad

Product Specs
ItemQX200 ™ Droplet Reader
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog Number1864003
DimensionsAutomated Droplet Generator dimensions (W x D x H): Open: 66 x 56 x 89 cm (26 x 22 x 35 in)
Closed: 66 x 56 x 66 cm (26 x 22 x 26 in)
QuantityEA
Capacity1–96 samples
Precision±10%
Sample Volume20 µl (Starting sample size)
ApplicationsDigital PCR
Wells96 wells

Specifications/Features

Droplets per 20 µl sample: 20,000

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Development of a qualitative detection method for the pSIM1278 construct in genetically modified potato using real-time PCRGM Crops FoodDecember 31, 2026Miyu Sugino, Keisuke Soga, Jumpei Narushima, Mika Matsumoto, Reona Takabatake, Satoko Yoshiba, Norihito Shibata30 s and 55°C for 1 min; and 98°C for 10 min (ramp rate of 2 °C/s). PCR products were read using a QX200 Droplet Reader (Bio-Rad Laboratories, Inc.) and analyzed using QuantaSoft Software QX Manager (version 1.2.345) (Bio-Rad

(2) Safety, efficacy, and distal nerve Schwann cell biodistribution in mice and NHPs to support translation of AAV9 RNAi therapy for CMT1AMolecular Therapy Nucleic AcidsJune 16, 2026Marina Stavrou, Lindsay M. Wallace, Merlin P. Thangaraj, Noah K. Taylor, Alexia Kagiava, Revekka Papacharalambous, Cynthia McAllister, Gloria Zender, Nizar Y. Saad, M. Bilal Bayazit, et al.set at 50%, and a final step at 60°C for 10 min. Data acquisition and analysis were performed on a QX200 Droplet Reader and QuantaSoft Software (Bio-Rad). The VGCN was calculated from absolute ddPCR quantification as a ratio

(3) Combination treatment with synthetic gRNA/Cas12a and gRNA/Cas9 ribonucleoproteins disrupts HIV replication and expressioniScienceMay 15, 2026Puja Banik, Ling Wang, Madison Schank, Jaeden S. Pyburn, Addison C. Hill, Yi Zhang, Juan Zhao, Holly K. Orfield, Tabitha O. Leshaodo, Janet W. Lightner, et al.final enzyme deactivation at 98 °C for 10 min, with a ramp rate of 2°C/s. Droplets were read using the QX200 Droplet Reader (Bio-Rad) with Direct Quantification of FAM fluorescence, and data were analyzed using QX Manager software

(4) Discovery of a chimeric transposase-transposon system for advanced genome engineeringiScienceMay 15, 2026Daniel Heinzelmann, Franziska Reuss, Nikolas Zeh, Robin Nilson, Emely Walker, Juergen Fieder, Benjamin Lindner, Benjamin Renner, Patrick Schulz, Simon Fischer, et al.pg of total RNA was used as template for the ddPCR reaction. Amplified samples were analyzed using a QX200 droplet reader (BioRad, Germany) for the determination of total fluorescent droplet counts. Based on the absolute quantification

(5) MEK inhibitor Mirdametinib promotes fracture healing in osteofibrous dysplasia RASopathyJournal of Clinical InvestigationMay 1, 2026Aysha B. Khalid, Kristin Denton, Nandina Paria, Ila Oxendine, Meghan Wassell, Reuel Cornelia, Sasidhar Uppuganti, Jeffry S. Nyman, G. Jayashree Jagadeesh, Carlos R. Ferreira, et al.accordance with the manufacturer’s instructions (Bio-Rad). The amplification signal was measured using a QX200 Droplet Reader, and data analysis was performed using QuantaSoft software, version 1.7.4 (Bio-Rad). Transcriptome

(6) Bioremediation of cadmium using cadmium-tolerant bacteria in cacao farms in Arauca, Colombia3 BiotechApril 23, 2026Laura Alvarez-Rubiano, Pedro Brito Brandão, Daniel Bravotemperature ramp of 2 °C each s. After the ddPCR copies were obtained, the plate was placed in the QX200 droplet reader (Bio-Rad), where the data were analysed with the Quanta Soft Analysis software (Bio-Rad Laboratories,

(7) Addressing a Flat‐Out Problem: Environmental DNA (eDNA) Exposes Silent Infestations of Acropora ‐Eating Flatworms ( Prosthiostomum acroporae ) in Coral AquacultureEcol EvolApril 17, 2026Clare M. Grimm, Jonathan Barton, David G. Bourne, Yui Sato, Jason Doylesimultaneously in a 96‐well PCR plate to minimize potential run‐to‐run biases. Droplets were read on a QX200 droplet reader (Bio‐Rad), and signal profiles were analyzed using Bio‐Rad QuantaSoft Analysis Pro software version

(8) Distinct Denitrification Phenotypes in Closely Related Bacteria: Clues to Understanding Variations in Nitrite Accumulation Among Stutzerimonas StrainsEnviron MicrobiolApril 16, 2026Martin Menestreau, Daniel A. Milligan, Louise B. Sennett, Linda Bergaust, Lars R. Bakken, Gary Rowley, Morten Kjos, James P. Shapleigh, Åsa Frostegårdstabilization at 4°C for 5 min and a final step at 90°C for 5 min. PCR products were quantified using a QX200 droplet reader (Bio‐Rad), and the data was analyzed using QuantaSoft Analysis Pro 1.0.596 (Bio‐Rad). All primers used

(9) The Integration of Focused Ultrasonication, ddPCR, and Flow Cytometry Effectively Estimates Genome Copies per Cell and Enhances DNA Extraction Efficiency in Escherichia coli SamplesACS OmegaApril 16, 2026Guilherme L. Pinheiro, Nancy J. Lin, Kirsten H. Parratt, Ian Hines, Holly R. Hack, Stephanie L. Servetas, Hariharan Iyer, Sandra M. Da Silvabefore a 4 °C hold. After reaction completion, the plate was removed and droplets were read on the QX200 Droplet Reader (BioRad, USA). Data analysis was performed using the QuantaSoft Analysis Software v1.7.4 (BioRad, USA).

(10) Enhanced Detection of Coccidioides spp. Fungi from Environmental Samples Using Droplet Digital PCREmerging Infectious DiseasesApril 16, 2026Jessica Paulette Segovia-Mota, Ricardo Eaton-González, Jimena Carrillo-Tripp, Meritxell Riquelmefollowed by inactivation at 90°C for 5 minutes. After amplification, we read the plate by using the QX200 Droplet Reader (Bio-Rad). We performed droplet analysis by using the QuantaSoft and QuantaSoft Analysis Pro software

(11) LRRC15-CAR T Cells for the Treatment of OsteosarcomaClinical Cancer ResearchApril 15, 2026Carla O’Reilly, Shraddha Tuladhar, Phuong Nguyen, Heather Sheppard, Selene C. Koo, Peter Chockley, Haley Houke, Teresa Santiago, Liqing Tian, Julie Jeschke, et al.mixture contained ddPCR Supermix for probes without UTP (Bio-Rad). The cycled droplets were read with the QX200 droplet reader (Bio-Rad). The ratio of the numbers of molecules of these two genes is the sample’s gene of interest

(12) Evaluating Sequencing Strategies for Endometrial Microbiome Profiling in Endometrial Cancer: A Comparative Study of Short- and Long-Read 16S rRNA ApproachesCancer Rep (Hoboken)April 14, 2026Sophia Bebelman, Anastasiia Artuyants, Bianca Nijmeijer, Sandra Fitzgerald, Claire Henry, Cherie Blenkiron94°C for 30 s and 59°C for 1 min; followed by enzyme deactivation at 98°C for 10 min and read using a QX200 Droplet Reader. Absolute quantification was performed using QuantaSoft Analysis Software (v1.7.4.0917). Positive controls

(13) Validation of a Duplex Digital PCR Assay for the Quantification of the NK603 Maize Event Across Three dPCR PlatformsFoodsApril 14, 2026Daniela Verginelli, Katia Spinella, Sara Ciuffa, Raffaele Carrano, Davide La Rocca, Elisa Pierboni, Monica Borghi, Silvana Farneti, Ugo Marchesistabilization step at 98 °C for 10 min and a hold at 4 °C. After amplification, droplets were read using a QX200™ droplet reader (Bio-Rad), and fluorescence data were processed with QX Manager software version 2.1 Standard Edition

(14) Predicting Recombinant mRNA Loading into Extracellular Vesicles: Insights from CD81 Fusion ConstructsInt J Mol SciApril 13, 2026Alessia Gabardi, Elena Gurrieri, Giulia Carradori, Dalia Tarantino, Vito Giuseppe D’Agostino(Bio-Rad). PCR was performed on the T100 Thermal Cycler (Bio-Rad), and droplets were detected using the QX200™ Droplet Reader (Bio-Rad Laboratories, Italy). To assess the relative distribution of specific transcripts into secreted

(15) Differential expression of POMC-processing genes in corticotroph tumorsEndocr OncolApril 9, 2026Elisa Lamback, Renan L Miranda, Laryssa Mendonça, Camila S de Figueiredo, Kaio C R Salum, Rômulo S Dezonne, Luiz Eduardo Wildemberg, Mônica R Gadelhalevels. Droplets were generated with the QX100 Droplet Generator (Bio-Rad) and amplified and read on QX200 Droplet Reader (Bio-Rad). Thermocycling conditions were 95°C for 10 min and 35 successive cycles at 95°C for 15 s and

(16) Comparative analysis of flavivirus sfRNA dynamics and secondary structurebioRxivApril 8, 2026Camden R. Bair, David VanInsberghe, Anice C. Lowen, Graeme L. Conn, Christopher J. Neufeldtminutes; and hold at 4 °C. Positive droplets were then detected on the QX200 Droplet Reader (Bio-Rad, 1864003). gRNA copies were determined by the positive droplets from the NS5 primer set. sfRNA copies were calculated

(17) Liver-Targeted AAV-DJ-hCBS Therapy Achieves Long-Term Correction of Metabolic Imbalance in CBS-Deficient MiceInt J Mol SciApril 7, 2026Christian P. Joschko, Chih-Chieh Wang, Azuwerus van Buiten, Maaike Goris, Femke Hoogstra-Berends, Joy Wang, Jan Henric Bacurio, Yinxing Chen, Nannan Jia, June Deng, et al.cycles of 94 °C for 30 s and 60 °C for 1 min; 98 °C for 10 min; 4 °C hold. Droplets were analyzed with a QX200 Droplet Reader, and copy number was calculated using QuantaSoft Analysis Pro version 1.7.4.0917 (Bio-Rad Laboratories,

(18) Development and evaluation of quadruplex droplet digital PCR assay for rapid detection of molecular markers associated with macrocyclic lactone resistance and susceptibility in Dirofilaria immitisInternational Journal for Parasitology: Drugs and Drug ResistanceApril 1, 2026Sohini Kumar, Isabelah Kassandra Lalicon, Roger K. Prichard, Thavy Longperformed on a C1000 Touch™ thermal cycler (Bio-Rad). Fluorescence signals were measured using the QX200™ Droplet Reader (Bio-Rad), and target concentrations were determined in copies/μl using Poisson's statistics. All reactions

(19) Clinical Validation of Digital PCR–Based ctDNA Detection for Risk Stratification in Residual Triple-Negative Breast Cancer: TRICIA Trial ResultsClinical Cancer ResearchApril 1, 2026Talia Roseshter, Anna Klemantovich, Josiane Lafleur, Cathy Lan, Luca Cavallone, Kathryn Bozek, Juliet Guay, Oluwadara Elebute, Sarah Jenna, Rodney Ouellette, et al.PX1 PCR Plate Sealer, cat. #1814000; C1000 Touch Thermal Cycler with 96–deep well reaction module, cat. #80224; and QX200 Droplet Reader, cat. #17005228; Bio-Rad) and ddPCR Supermix for probes (no UTP; cat. #Q33216, Bio-Rad). The final concentration

(20) Acquired On-Target Alterations Drive Clinical Resistance to p53-Y220C ReactivatorsCancer DiscoveryApril 1, 2026Ferran Fece de la Cruz, Andreas Varkaris, Parasvi S. Patel, Elijah W. Kushner, Alvin A. Morales-Giron, Sangmi Sandra Lee, Ankit Singh, Clara T. Kim, Bryanna L. Norden, Sara Ehnstrom, et al.assays) for 1 minute, followed by 98°C for 10 minutes (Ramp Rate 2°C/s). Droplets were analyzed using the QX200 Droplet Reader (Bio-Rad) for fluorescent measurement of FAM and HEX probes. Gating was performed based on positive

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