QuantStudio™ Absolute Q™ Digital PCR System from Thermo Fisher Scientific

Product Specs
ItemQuantStudio™ Absolute Q™ Digital PCR System
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog NumberA52864 / A53267
QuantityEA
ApplicationsLiquid biopsy mutation detection, rare mutation detection, copy number variation analysis, gene expression & miRNA analyses, pathogen detection, wastewater testing, gene therapy, genome editing, NGS library quantification
WellsMAP16 plate (up to 16 samples per run)
Dimensions(WxDxH) 600 x 540 x 620 mm (23.5 x 24.5 x 21.2 in) (Unpacked)
(WxDxH) 940 x 826 x 899 mm (37 x 32.5 x 35 in) (Packaged)
DetectionFour optical channels available (five optical channel system, ROX used for reference)
ThroughputUp to 16 samples per run. MAP16 plate can be run in intervals of four (i.e., 4, 8, 12, or 16)
Volume9 µl (< 5% dead volume)
Channels5 (1 reserved for QC)
Sample TypeDNA, RNA
Run TimeAs little as 90 minutes
DetectorFluorescence
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures.

Features

Background subtraction, machine learning false positive rejection

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Validation of a Duplex Digital PCR Assay for the Quantification of the NK603 Maize Event Across Three dPCR PlatformsFoodsApril 14, 2026Daniela Verginelli, Katia Spinella, Sara Ciuffa, Raffaele Carrano, Davide La Rocca, Elisa Pierboni, Monica Borghi, Silvana Farneti, Ugo Marchesiare expressed in copies of the target sequence per microliter of reaction. Eventually, cdPCR (e.g., QuantStudio Absolute Q Digital PCR System Thermo Fisher Scientific) reduces the workflow by eliminating droplet generation and emulsification

(2) Neuritin1 Cis-Regulatory Elements Enable Gene Expression Preferentially in Retinal Ganglion CellsInvest Ophthalmol Vis SciApril 9, 2026Venu Talla, Rajeshwari Koilkonda, Chaimaa Kinane, Moxa Panchal, Tamdan Khuu, Kevin K. ParkAbsolute Q Viral Titer dPCR Assay (Thermo Fisher Scientific, cat. A53736) on the Applied Biosystems QuantStudio Absolute Q Digital PCR System (Thermo Fisher Scientific). The final titers of AAVs ranged from 1 to 3 × 1013 genomic copies (gc)/mL. Experimental

(3) Bipolar CD4-targeted dual-DARPin-55/57 lipid nanoparticle enables efficient CRISPR/Cas-mediated HIV-1 DNA excision and reactivation blockade in latent CD4 T cell linesMaterials Today BioApril 1, 2026Subhra Mandal, Abdul Rasheed Baloch, Xinxu Yuan, Jackson Chen, A. Sami Saribas, Yuanjun Zhu, Danmeng Zhang, Dabbu Jaijyan, Jian Xu, Reafa Hossain, et al.LTR, Ψ packaging signal, and RRE regions were designed (Table 3). Digital PCR was performed on the QuantStudio Absolute Q Digital PCR System (Thermo Fisher Scientific) using the program: 96 °C for 10 min; 40 cycles of 96 °C for 5 s and 60 °C

(4) Extracellular Succinate Modulates Neuroimmune Responses in a Murine Microglial Cell LineBiomoleculesMarch 10, 2026Samantha C. Y. Yudin, Kimberly Day, Erica Y. Scott, Meha N. Patel, Hashim Islam, Andis KlegeriscDNA synthesis with the SuperScriptTM IV CellsDirectTM kit. Gene expression was quantified using the QuantStudio Absolute Q Digital PCR System, with the Absolute QTM Universal DNA Digital PCR Master Mix, 5× (A72710, ThermoFisher Scientific), Sucnr1

(5) Quantitative Analysis and Predictive Modeling of Nonspecific Adsorption of Recombinant Adeno-Associated Virus onto Solid SurfacesLangmuirMarch 10, 2026Yuki Ueda, Risa Shibuya, Koichi Shibata, Airi Murai, Yasuo Tsunaka, Mitsuko Fukuhara, Susumu UchiyamaDiscussion Section). Digital PCR Analysis rAAV genomic titers were quantified by digital PCR using the QuantStudio Absolute Q Digital PCR System (Thermo Fisher Scientific), according to a previously reported method. Samples were treated with DNase I

(6) Disrupted O-GalNAc glycosylation as a mechanism and biomarker of SLC35A2 -associated epilepsybioRxivMarch 4, 2026Robert G. Mealer, James J. Anderson, Sheridan L. Smith, Brianna M. Masters, Samuel H. Barth, Kaitlyn D. J. Huizar, Sahibjot Sran, Hyojung Yoon, Amanda Ringland, Savannah J. Muron, et al.in SLC35A2. Both the QuantStudio 3D Digital Real-Time PCR system (Thermo Fisher Scientific) and the QuantStudio Absolute Q Digital PCR System (Thermo Fisher Scientific) were used to quantitatively assess allele fraction per the manufacturer’s

(7) RNU2‑1 gene copy number variations do not affect serum levels of miR‑1246 as a biomarker for lung adenocarcinomaExperimental and Therapeutic MedicineFebruary 27, 2026Makiko Ueda, Shinya Sato, Yohei Miyagi, Toshiko Aisoaccording to the cycling protocol: 96˚C for 10 min, 40 cycles of 96˚C for 5 sec, and 61˚C for 30 sec, on a QuantStudio Absolute Q Digital PCR system (Thermo Fisher Scientific Inc.). The sequences of all TaqMan probes and primers are listed in Table

(8) Generation and characterization of human induced pluripotent stem cells from neuropathologically confirmed multiple system atrophy patient-derived fibroblastsFrontiers in ImmunologyFebruary 23, 2026Mireia Alemany-Ribes, Alexandra Pérez-Soriano, Almudena Santos, Jacqueline Severino, Adrià Dangla-Valls, Martín Gigirey-Suárez, Dory Cohen, Mario Ezquerra, Ruben Fernandez-Santiago, Manel Fernandez, et al.was added on top of each well to prevent contamination and evaporation. The plate was loaded into the QuantStudio Absolute Q™ Digital PCR System (ThermoFisher Scientific) and the amplification conditions were as follows: 96 °C for 10 min; 40 cycles

(9) Development of a Duplex Digital PCR and Validation on eDNA Water Samples for Monitoring of the Asian Swamp Eel ( Monopterus albus / Javanensis ) and Bullseye Snakehead ( Channa aurolineata / Marulius ) in Florida, USA, Freshwater EcosystemsEcology and EvolutionFebruary 17, 2026Melody Bloch, Eric Suarez, Melissa A. Miller, Sergio A. Balaguera‐Reina, Cynthia A. Fussell Persaud, Kevin A. Olejniczak, Ericka E. Helmick, Frank J. Mazzotti, Brian W. Bahdercopies/μL. Serially diluted plasmids for BS and ASE were subsequently run with the corresponding assay on the QuantStudio Absolute Q Digital PCR System (dPCR; ThermoFisher Scientific) to establish optimal dilution concentrations. In addition, the eluate

(10) Chromosome centromere copy number amplification associated with exceptional response in HER2-positive metastatic breast cancer patientsOncogeneFebruary 10, 2026Charlotte Andrieu, Jojanneke Stoof, Dalal AlSultan, Laura Ivers, Jose Javier Berenguer Pina, Darko Skrobo, Jo Ballot, Debbie O’Reilly, Denis M. Collins, Alex J. Eustace, et al.instructions, and at the following cycling steps: 10 min at 96 °C, 40x (5 min at 96 °C, 15 s at 60 °C)(QuantStudio Absolute Q Digital PCR System, Thermo Fisher Scientific). D4Z1 copy number was normalized to the reference genes RHOT1 (chr17) and

(11) Neuroinflammatory and transcriptional dynamics during SARS-CoV-2 infection in KRT18-hACE2 mouse brainFront ImmunolFebruary 2, 2026Dae-Gyun Ahn, Nhu Thi Quynh Mai, Da-Jin Jeong, Byoung-San Moonsample was performed using Absolute Q™ DNA Digital PCR Master Mix (ABI, Thermo Fisher Scientific) and QuantStudio Absolute Q Digital PCR System (ABI) following manufacture’s procedure. The primers used for dPCR were provided in Supplementary Table

(12) Advances in Functional Foods: Using Double Emulsion Gels to Deliver CBD and Probiotics and to Modulate Human Gut Microbial CommunitiesNutrientsJanuary 23, 2026Sigita Jeznienė, Ina Jasutienė, Milda Keršienė, Rita Bandariavičiūtė, Laurita Varnaitė-Kapočė, Ieva Bartkuvienė, Vida Audra Budrienė, Arūnas Jonušas, Daiva Leskauskaitė, Aušra Šipailienėprimers and probe, and 5 μL of DNA, was prepared. The dPCR was performed in a total volume of 9 μL on a QuantStudio™ Absolute Q™ Digital PCR System (Thermo Fisher Scientific, Forster City, CA, USA) using its proprietary microfluidic array plate (MAP).

(13) Auranofin, identified by FDA-approved drug library screening, inhibits HBs antigen secretion via lysosomal damagePLOS OneJanuary 16, 2026Akiyoshi Shimoda, Kazuhiro Murai, Hayato Hikita, Satoshi Minami, Takayuki Miyake, Shinji Kuriki, Emi Sometani, Jihyun Sung, Satoshi Shigeno, Yuichiro Higuchi, et al.Assays (Thermo Fisher Scientific, Waltham, MA, USA). The level of cccDNA was analyzed by ddPCR using the QuantStudio Absolute Q digital PCR system (Thermo Fisher Scientific Waltham, MA, USA). Before analysis, the isolated DNA was digested with Plasmid-Safe

(14) Methylation Biomarker of Chronic Heavy Alcohol Consumption (HAC), but Not Acute HAC, Predicts All-Cause Mortality in Prostate, Lung, Colorectal and Ovarian Cancer Screening TrialGenes (Basel)January 6, 2026Steven R. H. Beach, James A. Mills, Jeffrey D. Long, Robert A. Philibertcg05575921 (Behavioral Diagnostics, Coralville, IA, USA) in combination with digital PCR reagents and a QuantStudio Absolute Q Digital PCR System from ThermoFisher (Hercules, CA, USA). Then, methylation values ((C/C + T) ratios) were determined using

(15) Detection of Echinococcus granulosus sensu lato microRNAs in cystic echinococcosis patients: An exploratory study using quantitative PCR and digital PCRPLoS Negl Trop DisDecember 15, 2025Chiara Stocchero, Tommaso Manciulli, Alessandro Zanon, Gaspare Salvi, Susanna A. Sechi, Alessia Pea, Alessandra Cafiso, Paola Pepe, Ambra Vola, Marcela A. Cucher, et al.evaluate the circulation of E. granulosus s.l.-specific miRNAs in serum of CE patients, a dPCR using QuantStudio Absolute Q Digital PCR System (Thermo Fisher Scientific, Waltham, MA, USA) was performed following the Digital MIQE Guidelines [37].

(16) Variation of VP2 stoichiometry and deamidation of VP1 during production and their impacts on the transduction efficiency of AAV vectorsMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Takahiro Maruno, Mitsuko Fukuhara, Yasuo Tsunaka, Aoba Matsushita, Kiichi Hirohata, Karin Bandoh, Megumi Onaka, Risa Shibuya, Yuki Yamaguchi, Haruka Nishiumi, et al.water, and QuantStudio Absolute Q DNA Master Mix (Thermo Fisher Scientific). dPCR was performed using a QuantStudio Absolute Q Digital PCR System and 16-well microfluidic array plates (Thermo Fisher Scientific). The dPCR sequence consisted of enzyme

(17) Expression of Glutamatergic Signaling in Canine Oral Melanocytic NeoplasmsVet SciDecember 2, 2025Alison Masyr, Latticha Pluemhathaikij, Sichao Wang, Tuddow Thaiwong-Nebelung, Rebecca C. SmedleyμL of QuantStudio Absolute Q Isolation Buffer overlay. The reaction was run in the thermal cycler (QuantStudio Absolute Q Digital PCR System, Thermo Fisher Scientific, Waltham, MA, USA) under the following conditions: 10 min at 96 °C, and 40

(18) Replication-driven HBV cccDNA loss in chimeric mice with humanized liversJournal of VirologyNovember 25, 2025Bai-Hua Zhang, Yuanping Zhou, Stephen Horrigan, Fabien Zoulim, Jianming Hu, Yong-Yuan Zhangcopies/cell initially computed by qPCR, using the ThermoFisher Absolute Q Digital PCR system (cat. no. A52864). Briefly, a 9.1 µL reaction mix was prepared consisting of 1.8 µL of 5 × DNA dPCR mix (ThermoFisher

(19) Tracking leukemic residuals: dissecting the inverse relationship between CD26+ stem cells and extracellular BCR::ABL1 transcript in Chronic Myeloid Leukemia (CML)Stem Cells Translational MedicineNovember 24, 2025Silvia Mutti, Alessia Cavalleri, Anna Sicuranza, Paola Pacelli, Claudia Ielo, Lucia Paolini, Valentina Mangolini, Alessandro Leoni, Teresa Miracapillo, Camilla Turriziani, et al.EVES BCR::ABL1 (Hs03024541_ft, FAM-MGB) transcript was quantified via digital PCR (dPCR) using the QuantStudio Absolute Q Digital PCR System (Life Technologies). Briefly, 5 μL of EVES cDNA were loaded on a dPCR plate together with 1.8 μL of

(20) H3K4me3 amplifies transcription at intergenic active regulatory elementsGenes & DevelopmentNovember 3, 2025Haoming Yu, Yongyan Zhang, Zhicong Liao, Benjamin William Walters, Bluma J. Leschtranscription was carried out using random hexamers. Digital droplet PCR was performed on a Fisher QuantStudio Absolute Q digital PCR system according to the manufacturer's instructions. TT-TimeLapse-seq was performed as described previously

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