Wizard DNA Clean-Up System from Promega

Product Specs
ItemWizard DNA Clean-Up System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA7280
TimeResults in 15 minutes or less.
Methodquick batch column procedure
Quantity100 preps
SourceReaction, Bacterial
Nucleic Acid TypeGenomic DNA, Plasmid DNA, DNA Fragment, PCR Product
Sample TypeEnzymatic reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Eggerthella lenta evades bacteriophage through reversible megabase-scale inversions of capsular polysaccharide gene clustersbioRxivMay 1, 2026Shenwei Zhang, Colin Buttimer, Kai R. Trepka, Kathy N. Lam, Luis A. Ramirez Hernandez, Paola Soto-Perez, Cecilia Noecker, Paolo Canigiula, Edwin F. Ortega, Jenny Lee, et al.following a modified Wizard DNA Clean-Up protocol developed by the Center for Phage Technology (Promega A7280). Briefly, 10 mL of phage lysate was treated with DNase I and RNase A, gently mixed, and incubated at

(2) Annotated genome sequence of temperate Arthrobacter globiformis phage, JamunMicrobiology Resource AnnouncementsApril 9, 2026John Horangic, Julia Wright, Ashna Siddiqui, Claire Bartley, Cynthia Brogan, Annie Katanga, Mel Buzzanga, Kristen Johnson, Kyle MacLeahigh-titer lysate (HTL) with a concentration of 9 * 109 pfu/mL (1). The Promega Wizard DNA Clean-Up Kit (A7280, Promega, Madison, WI, USA) was used to extract the DNA from the HTL (1). Libraries were prepared using

(3) An A-rich linker between dengue virus tandem xrRNAs facilitates functional coordinationbioRxivMarch 4, 2026Elizabeth Spear, Zoe O'Donoghue, Steve L. Bonilla, Kevin P. Larsen, Madeline E Sherlock, Jeffrey S. Kieftcleaned up using Promega’s Wizard DNA cleanup kit according to the manufacturer’s protocol (Promega A7280). Viral infections of mammalian and mosquito cells and Northern blot analysis. Vertebrate cells were

(4) FMR1 RNA interaction with DNMT1 blocks DNA methylation at the FMR1 locusNAR Molecular MedicineJanuary 14, 2026Veronica Nobile, Benedetta Niccolini, Cecilia Pucci, Roberta Pietrobono, Clarissa Modafferi, Carla Lucia Esposito, Natalia Cappoli, Giada Bianchetti, Giuseppe Maulucci, Giuseppe Aceto, et al.input and RNaseH digested samples) was purified through Wizard® DNA Clean-Up system (Promega, cat. A7280). DNA samples were quantified by absolute qPCR (ABI7900HT, Life Technologies) using fluorescent probes

(5) Genome Sequences of Alatato and Audell, two Phages that infect Arthrobacter globiformis and have Different Life CyclesMicroPubl BiolDecember 3, 2025Sarah E Robinson, Erin Stanford, Shilen Abraham, Rebecca Aguilera, Nihal S Anantoji, Marco Barillas, Ryan Barstys, Emily Benson, Kayce R Boucher, Adrianna M Chacko, et al.PFU/ml) were prepared from these plates and genomic DNA was isolated using the Wizard DNA clean-up kit (A7280; Promega). Genomic DNA was used to create sequencing libraries with the NEB XLEP-P1 kit. Sequencing

(6) Closed genome sequence of an Arthrobacter globiformis phage, SilentRXMicrobiology Resource AnnouncementsOctober 9, 2025Ashna Siddiqui, Raunak Vijay, Ethan G. Edwards, Meliha Ulker, Fardeen A. Siddiqui, Thomas J. Gerton, Rachel E. Anastasi, Dylan J. Conroy, Isabelle E. Laizure, Joshua D. Reynolds, et al.respectively (Fig. 1). DNA was extracted from high-titer lysate using the Wizard DNA Clean-Up Kit (A7280, Promega, Madison, WI, USA). A sequencing library prepared for SilentRX using the Ultra II FS kit (E7805S,

(7) Valemetostat–SAHA-Driven Acetylation of p53 via SET/TAF-Iβ Displacement and p300 Activation Modulates Cell Cycle Regulators in Pancreatic Cancer CellsBiomedicinesSeptember 17, 2025Michele Di Crosta, Francesca Chiara Ragone, Rossella Benedetti, Gabriella D’Orazi, Roberta Santarelli, Maria Saveria Gilardini Montani, Mara Cironesamples were cleaned up with the Wizard DNA Clean-UP System (Promega, Madison, Wisconsin, USA, cat n. A7280). The p53 binding to p21 was evaluated by qRT-PCR using specific primers for the p21 promoter region,

(8) A paper-based loop-mediated isothermal amplification assay for highly pathogenic avian influenzaSci RepApril 9, 2025Mohamed Kamel, Josiah Levi Davidson, Jenna M. Schober, Gregory S. Fraley, Mohit S. Vermatemplate was then subjected to a plasmid purification step using the Wizard Clean-up System (Promega A7280; Madison, WI, USA). The purified linearized DNA, including the T7 promoter, was then used as a template

(9) Characterization and genomic analysis of Sharanji: a jumbo bacteriophage of Escherichia coliVirol JMarch 10, 2025Sharayu Magar, Sivaraj Barath, Debmitra Sen, Ranjith Kumar Singari, T. Nagarajan, Anjali Parmar, Sutharsan GovindarajanSharanji was isolated from the lysate using the Promega Wizard DNA Clean Up Kit (Promega catalogue #A7280) according to the manufacturer’s instructions and as described previously [13]. The extracted DNA samples

(10) Phylogeny and Functional Differentiation of the Terpene Synthase Gene Family in Angiosperms with Emphasis on Rosa chinensisInternational Journal of Molecular SciencesFebruary 27, 2025Qi Li, Yifang Peng, Tao Zhao, Qijing Dong, Qian Yang, Xiaoyu Liu, Yu HanMaterials Commercial reagents included: Plant RNA Purification Reagent (Promega, Madison, WI, USA, Cat# A7280); PrimeScript RT Reagent Kit with gDNA Eraser and SYBR Premix Ex Taq (Takara Bio, Shiga, Otsu, Japan,

(11) Metagenomic time series reveals a Western English Channel viral community dominated by members with strong seasonal signalsThe ISME JournalOctober 23, 2024Luis M Bolaños, Michelle Michelsen, Ben Temperton(100 U/ ml; 2 h at room temperature). DNA was extracted using the Wizard DNA Clean-Up System (Promega A7280) and cleaned with a 1.5 × Ampure bead cleanup. 50 ng of DNA were used to construct sequencing libraries

(12) Nine Cluster E mycobacteriophages isolated from soilMicrobiology Resource AnnouncementsOctober 10, 2024Joseph M. Gaballa, Amanda Freise, Krisanavane Reddi, Jordan Moberg Parkerby the SEA-PHAGES Discovery Guide (5). DNA was isolated using the Wizard Promega DNA Clean-Up Kit (#A7280). Pooled libraries were prepared with a NEB Ultra II DNA Library Prep kit (NEB #E7103) for Illumina sequencing

(13) Complete genomes of two cluster AK Arthrobacter phages isolated from soil samples in Newburgh, NY, United StatesMicrobiology Resource AnnouncementsOctober 10, 2024Véronique A. Delesalle, Mariah A.K. King, Tabitha J. Rozario, Noah D. Wolf, Connor J. Stewart, Luke F. DeMato, Kevin F. Trafford, Saiman Adhikari, Van T. Dinh, Gina Caputo, et al.of plaques. For each phage, DNA was extracted from HTL using the Promega Wizard DNA CleanUp System (A7280) and sequenced at the Pittsburgh Bacteriophage Institute. DNA libraries were prepared with the NEB Ultra

(14) Genome sequence of Xenia2 a DV cluster phage that infects Gordonia rubripertinctaMicrobiology Resource AnnouncementsSeptember 10, 2024Carol Agaiby, Maha Ahmed, Aidan Argueta, Kyle Arrowood, Keelynn P. Barrier, Meghan W. Church, Cheryl R. Connell, Ken D. Dao, Kathleen Huyen T. Dao, Makenzie R. Davenport, et al.a high-titer filtrate after three rounds of plaque purification using the Wizard DNA clean-up kit (A7280; Promega). Genomic DNA was used to create sequencing libraries with the NEB Ultra II Library Kit, v3

(15) Long-read powered viral metagenomics in the oligotrophic Sargasso SeaNature CommunicationsMay 14, 2024Joanna Warwick-Dugdale, Funing Tian, Michelle L. Michelsen, Dylan R. Cronin, Karen Moore, Audrey Farbos, Lauren Chittick, Ashley Bell, Ahmed A. Zayed, Holger H. Buchholz, et al.remove un-encapsulated DNA). Viral DNA was extracted using the Wizard® DNA Clean-up System (Promega A7280). In contrast to previous samples derived from a coastal station (Western English Channel site L423;

(16) Genomic and Phenotypic Analysis of Salmonella enterica Bacteriophages Identifies Two Novel Phage SpeciesMicroorganismsMarch 29, 2024Sudhakar Bhandare, Opeyemi U. Lawal, Anna Colavecchio, Brigitte Cadieux, Yella Zahirovich-Jovich, Zeyan Zhong, Elizabeth Tompkins, Margot Amitrano, Irena Kukavica-Ibrulj, Brian Boyle, et al.Annotation Genomic DNA from the Salmonella phages was extracted using the Wizard DNA Clean-Up system (A7280; Promega, Madison, WI, USA) following the modified Promega Wizard method, as described by the Center

(17) A universal system for boosting gene expression in eukaryotic cell-linesNat CommunMarch 16, 2024Inbal Vaknin, Or Willinger, Jonathan Mandl, Hadar Heuberger, Dan Ben-Ami, Yi Zeng, Sarah Goldberg, Yaron Orenstein, Roee Amitenzymatic reactions using Promega Wizard DNA Clean-Up System according to manufacturer’s protocol (Promega #A7280). Bacterial transformation and OL plasmid purification We used 25 µl E. cloni 10G CLASSIC Electrocompetent Cells

(18) Cyclin B2 impairs the p53 signaling in nasopharyngeal carcinomaBMC cancerJanuary 2, 2024Liu Q, Yuan Y, Shang X, Xin L.with about 50 µL mineral oil at 50 °C for 16 h. After removing the oil layer, 1 mL DNA Wizard reagent (A7280, Promega) was supplemented to the elution column, and the mixture was eluted with isopropanol and incubated

(19) Evolution of Cherries (Prunus Subgenus Cerasus) Based on Chloroplast GenomesInternational Journal of Molecular SciencesOctober 26, 2023Xin Shen, Wenjin Zong, Yingang Li, Xinhong Liu, Fei Zhuge, Qi Zhou, Shiliang Zhou, Dongyue Jiangcetyltrimethylammonium bromide (mCTAB) protocol [54] and purified using the Wizard DNA Clean-Up System (A7280, Promega Corporation, Madison, WI, USA). DNA concentration was assessed using the Qubit 2.0 Fluorometer

(20) Annotation of Secretariat and Hydrus, two DJ cluster phages isolated on Gordonia rubripertinctaMicrobiology Resource AnnouncementsOctober 19, 2023Jackson Bland, Sydney Miller, Elijah D. Algarin-Martinez, Abbigail M. Biggs, Maria E. D. Cavasini, Michael A. Chase, Caitlyn Coleman, Victoria Correa, Don F. Danielson, Wynter R. Dean, et al.Genomic DNA was isolated after three rounds of plaque purification using the Wizard DNA clean-up kit (A7280; Promega). Genomic DNA was used to create sequencing libraries with the NEB Ultra II Library Kit, v3

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