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Assay Kits
Assay Kit
Agilent DNA 1000 Kit
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Agilent DNA 1000 Kit
Agilent DNA 1000 Kit from Agilent Technologies
(1)
Write a review
Citations:
(769)
Figures:
(11)
Supplier Page
Supplier Page from
Agilent Technologies for
Agilent DNA 1000 Kit
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Description
For the separation, sizing, and quantification of dsDNA fragments from 25 to 1000 bp. The kit includes 25 microfluidic chips, reagents and consumables, sufficient for 300 samples.
Product Specs
Company Info
Citations
Reviews
Protocols
Product Specs
Item
Agilent DNA 1000 Kit
Company
Agilent Technologies
Price
Supplier Page
View Company Product Page
Catalog Number
5067-1504
Assay Time
35 min
Regulatory Status
RUO
Detection Target
DNA
Quantity
1 kit
Number of Samples
300
Type
DNA 1000 Assay
Detection Range
Quantitative Range : 0.5 to 50 ng/µL
Sizing Range : 25 to 1000 bp
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Agilent Technologies
5301 Stevens Creek Blvd
Santa Clara
,
California
95051
United States
Phone
: +1 877-424-4536
Company Profile
Website:
www.agilent.com
Citations (769)
(1)
MECP2 mutations rewire human ESC fate and bias cortical lineage commitment
Stem Cell Reports
May 12, 2026
Marion Guillon, Margaux Brin, Elodie Gabet, Justine Gromaire, Mathéa Bernard, Laetitia Laurent, Théo Rabin, Lisa Bianchin, Marie Veziano, Julie Kloda,
et al.
#
E2670
), and fragment size distribution verified on an
Agilent
2100 Bioanalyzer
using the DNA 1000 kit (#
5067-1504
). Libraries were sequenced on an Illumina NovaSeq 6000 S4 flow-cell using paired-end 150-bp reads (PE150). Single-cell
(2)
Construction of a randomly barcoded insertional mutant library in the filamentous fungus Trichoderma atroviride
bioRxiv
April 21, 2026
Lori B. Huberman, José M. Villalobos-Escobedo, Jeffrey M. Skerker, Ran Shi, Adriana M. Rico-Ramírez, Catharine Adams, Adam P. Arkin, Adam M. Deutschbauer, N. Louise Glass
were assessed using an
Agilent
2100 Bioanalyzer
with the DNA 1000 Kit (
Agilent Technologies
, cat. no.
5067-1504
). Only libraries displaying a clear peak around 400 bp and minimal adapter contamination were retained
(3)
Cross-subgenus hybridization between Leishmania and Sauroleishmania informs on parasite genomic compatibility and transcriptomic adaptation
bioRxiv
April 12, 2026
Viviane Noll Louzada-Flores, Pascale Pescher, Thomas Cokelaer, Tiago Rodrigues Rodrigues Ferreira, Maria Stefania Latrofa, Jairo Alfonso Mendoza-Roldan, Domenico Otranto, Gerald F Späth, Isabelle Louradour
instructions. The quality and concentration of RNA were measured by Bioanalyzer DNA1000 Chips (
Agilent
, #
5067-1504
). Transcriptomic
sequencing
data were generated using an Illumina NextSeq 2000 platform, producing single-end
(4)
Serum exosomal miR-192-5p as a novel predictive biomarker for immunochemotherapy response in advanced non-small cell lung cancer
BMC Cancer
April 1, 2026
Yi-Fei Chen, Chu-Lin Zhang, Yuan Li, Chao-Feng Liu, Hui-Yu Jiang, Yong Yang, Hai-Long Wang, Min Pang
Library quality was evaluated on an
Agilent
2100 Bioanalyzer
with the High
Sensitivity
DNA Kit (Cat. No.
5067 − 1504
,
Agilent
, USA), and quantification was performed by
qPCR
using the Kapa Library Quantification Kit (Cat.
(5)
Metabolic beneficial effects of targeting a long non-coding RNA, lnc-megacluster, in obesity
Molecular Therapy Nucleic Acids
March 12, 2026
Maryam Abdollahi, Vajir Malek, Vinay Singh Tanwar, Mitsuo Kato, Linda Lanting, Alireza Rezaei, Lingxiao Zhang, Lixin Yang, Raju K. Pillai, Leah Kebrom,
et al.
(MAN-10153-06). The quality of the library was assessed by the Bioanalyzer DNA 1000
assay
(
Agilent
, catalog
5067-1504
). The library was then sequenced on Illumina NextSeq2000. The fastq files were converted to DCC files
(6)
Genomic instability and mono-parental expression mitigate genomic shock in a cross-subgenus Leishmania hybrid
NAR Mol Med
March 11, 2026
Viviane Noll Louzada-Flores, Thomas Cokelaer, Marcela Fuentes-Carias, Pascale Pescher, Tiago Rodrigues Ferreira, Maria Stefania Latrofa, Jairo Alfonso Mendoza-Roldan, Domenico Otranto, Gerald F Späth, Isabelle Louradour
instructions. The quality and concentration of RNA were measured by Bioanalyzer DNA1000 Chips (
Agilent
, #
5067-1504
). Transcriptomic
sequencing
data were generated using an Illumina NextSeq 2000 platform, producing single-end
(7)
Dysregulated interferon signaling and hyperinflammation upon FCGBP knockdown in goat bronchial epithelial cells infected with Pasteurella multocida
Front Vet Sci
March 2, 2026
Yingxue Yang, Xiangyin Chen, Ziying Wang, Lili Wu, Li Du, Hongyan Gao, Si Chen, Qiaoling Chen, Fengyang Wang
libraries, which were then subjected to quality control using the DNA 1000
Assay
Kit (
Agilent Technologies
,
5067-1504
, USA). The libraries with sufficient quality were subsequently sequenced on an
Illumina
NovaSeq X Plus
(8)
Disruption of neutrophil homeostasis is associated with functional alterations in mitochondria of critically ill COVID-19 patients
Sci Rep
March 1, 2026
Aya A. Elkhodiry, Basma A. Yasseen, Hajar El-Sayed, Mona Zidan, Azza G. Kamel, Rehab Hamdy, Sara Gohar, Mohamed A. Badawy, Aya Saber, Hend E. El-Shqnqery,
et al.
distribution of the pooled libraries was analyzed using a Bioanalyzer DNA
assay
(
Agilent
, USA; Cat. No.:
5067−1504
) and the concentration was assessed using the Qubit dsDNA HS
Assay
(ThermoFisher Scientific, USA; Cat.
(9)
Dahuang Zhechong pill alleviates pulmonary fibrosis by inhibiting apoptosis and epithelial-mesenchymal transition of type II alveolar epithelial cells in vitro and in vivo
Journal of Traditional and Complementary Medicine
March 1, 2026
Fang Sun, Zhaochu Wu, Song Cai, Xiaoming Xing, Jing Wang, Lishan Zhang
Technologies, USA. The Bioanalyser 2100 RNA-6000 Nano Kit (#
5067-1511
) and Bioanalyser 2100 DNA-1000 Kit (#
5067-1504
) were purchased from
Agilent
, USA. Fetal bovine serum (#11011-8611) was purchased from Hangzhou Sijiqing
(10)
Epigenetic landscape of the H3K27me3 mark in macrophages transformed by Theileria annulata
Communications Biology
February 24, 2026
Takaya Sakura, Shahin Tajeri, Zineb Rchiad, Hifzur R. Ansari, Abhinav Kaushik, Tobias Mourier, Arnab Pain, Michel Wassef, Gordon Langsley
validation was conducted using the 1000 DNA kit on
2100 Bioanalyzer
(
Agilent Technologies
, catalog number
5067-1504
) and quantified using Qubit (
Thermo Fisher Scientific
, catalog number
Q32850
). ssRNA libraries were
(11)
CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization
Nature Communications
February 21, 2026
Tomoya Honda, Sora Yu, Dung Mai, Leo Baumgart, Emory M. Chan, Gyorgy Babnigg, Yasuo Yoshikuni
740609.050). The quality of the amplicon samples was validated using Bioanalyzer
DNA Analysis
(
Agilent
:
5067-1504
). For RNA samples, 70 µl of purified RNA was treated with DNase (Thermo Scientific: AM1907) to remove
(12)
Silk-trehalose seed coating technology preserves Rhizobium tropici viability and enhances zinc biofortification in common bean under marginal soil conditions
Frontiers in Plant Science
February 19, 2026
Manal Mhada, Salma Mouhib, Khaoula Errafii, Adil El Baouchi, Oumaima Zaher, Dachena Romain Gracia, Augustine T. Zvinavashe, Lamfeddal Kouisni, Benedetto Marelli
Fragment size was determined using Agilent’s Bioanalyzer instrument using
Agilent
DNA 1000
assay
kit Cat #
5067-1504
.
PCR reactions
contained 1 ng of DNA, and KAPA HiFi HotStart Ready Mix. The Ready Mix contains
KAPA HiFi
(13)
NR4A1 mediates chemotherapy‑induced senescence via the PI3K/AKT pathway in gastric cancer cells
Oncology Reports
February 18, 2026
Tingyu Zhang, Yue Wang, Jiuna Zhang, Xueshuai Ye, Yanfeng Shen, Zhiwei Zhang
were then amplified by
PCR
. Library concentration was evaluated using the DNA1000
assay
Kit (cat. no.
5067-1504
;
Agilent Technologies
, Inc.), and the final library loading concentration was 3 ng/µl.
Sequencing
was
(14)
Deep learning-based non-invasive profiling of tumor transcriptomes from cell-free DNA for precision oncology
bioRxiv
February 16, 2026
Robert D. Patton, Alexander Netzley, Thomas W. Persse, Akira Nair, Patricia C. Galipeau, Ilsa M. Coleman, Pushpa Itagi, Pooja Chandra, Mohamed Adil, Manasvita Vashisth,
et al.
Q32851
), and size distribution assessed on a Bioanalyzer using the DNA 1000 or HS DNA 1000 Kit (
Agilent
,
5067–1504
or
5067–4626
). Plasma cfDNA libraries were prepared with either the
NEBNext Ultra II DNA Library Prep Kit for Illumina
(15)
Coevolution and Functional Effects of Endosymbiotic Rickettsia in Leptocybe invasa Fisher & LaSalle (Hymenoptera: Eulophidae) Across China
Ecol Evol
February 10, 2026
Xiu Xu, Leming Zhou, Jinting Xie, Junjue Li, Chunhui Guo, Zhende Yang
sequencing
adapters. Library quality was assessed with the DNA 1000
Assay
Kit (
Agilent Technologies
,
5067‐1504
), and libraries passing quality control were sequenced in a paired‐end format on the Illumina HiSeq
(16)
Integrative functional genomics analysis identifies pleiotropic genes for vascular diseases
Nature Communications
February 5, 2026
Charles U. Solomon, David G. McVey, Catherine Andreadi, Peng Gong, Lenka Turner, Dedrick S. S. Song, Heming Zhang, Dominic P. Lee, Elisavet Karamanavi, Wei Yang,
et al.
Qubit dsDNA HS Assay Kit
(
ThermoFisher Scientific
,
Q32851
) and the quality assessed with DNA 1000 kit (
Agilent
,
5067-1504
) on the
2100 Bioanalyzer
System (
Agilent
). Subsequently, libraries with barcoded Rev-1 to Rev-4 were
(17)
CD4+CXCL13+ exhausted T cells drive immune microenvironment divergence in synchronous double primary lung adenocarcinoma with different degrees of invasiveness
Translational Lung Cancer Research
January 31, 2026
Xueyu Chen, Jiahao Zhang, Tong Lu, Mingyuan Du, Fangyuan Li, Dong Dong, Yuqin Cao, Yajie Zhang, Hecheng Li
(
Thermo Fisher Scientific
). Fragment size was evaluated using the
Agilent
2100 Bioanalyzer
with the DNA HS Kit (cat. No.
5067-1504
/
5067-1511
,
Agilent Technologies
, Santa Clara, CA, USA). DNA was fragmented into 200–250 bp fragments
(18)
Inhibition of SPI1 by ADAP Regulates S100A8/A9 Signaling in Macrophages to Control the Development of Colitis
Inflammation
January 6, 2026
Yanqi Wang, Xiao Li, Xinyue Lv, Pengchao Zhang, Hebin Liu
manufacturer’s protocol. Library quality was assessed using a DNA 1000
Assay
Kit (
Agilent Technologies
,
5067 − 1504
, China).
RNA sequencing
was performed by Guangzhou Genedenove Corp, and bioinformatic analyses were
(19)
Identifying actionable genetic mutations and microsatellite instability in liquid biopsy of colorectal cancer
Journal of Genetic Engineering and Biotechnology
December 1, 2025
Neemat M. Kassem, Mohamed G. Seadawy, Maha Gaafar, Hebatallah A. Kassem, Amira Farouk Ahmed Hussein, Enas M. Ali Rizk, Marwa A. Hassan, Riham H. AbdelAziz, Mentallah M. Abdelradi, Mostafa F. El-Hosseny,
et al.
utilizing the DNA 1000 reagents & Chips (
Agilent Technologies
, Santa Clara, California, Cat. Code:
5067–1504
) with expected
PCR product
186–277 bp. Patients' libraries as well as PhiX control library were normalized
(20)
Bisphenol B Exposure Induces Miscarriage by Suppressing Migration/Invasion and Migrasome Formation
Advanced Science
November 21, 2025
Wenxin Huang, Manli Wang, Yi Sun, Xiaoping Yue, Xun Li, Yanbing Lin, Geng Guo, Depeng Zhao, Qigang Fan, Xiaoling Ma,
et al.
software. The libraries for quality control were built using the DNA 1000
assay
Kit (
Agilent Technologies
,
5067‐1504
) following the criteria: 1) concentration >2 ng µL−1, 2) a single peak shape with a size of 300–600
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Reviews
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(1)
9/12/2016
A Good Method For Quantification Of Semi Quantitative PCR Product
sndp kmr
In semi quantitative PCR method of gene quantification the end product of PCR have to analysed on the Agarose gel. Unfortunately small amplicons of size less the 100bp cannot be visualised on the...
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Protocols
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April 01, 2013
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January 01, 2010
Agilent DNA 1000 Kit from Agilent Technologies
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