NEBNext Quick Ligation Module from New England Biolabs

Product Specs
ItemNEBNext Quick Ligation Module
CompanyNew England Biolabs
Price $344.00 Inquire
Catalog NumberE6056S
Quantity20 reactions
ApplicationsDNA Analysis, Ligation, NGS
Temperature-20 C

Specifications/Features

Information:
The Module Includes:

The volumes provided are sufficient for preparation of up to 20 reactions (NEB #E6056S) and 100 reactions (NEB #E6056L). (All reagents should be stored at -20 Degrees C).

Quick T4 DNA Ligase

#E6057A: 0.1 ml

#E6057AA: 0.5 ml

NEBNext Quick Ligation Reaction Buffer (5X)

#E6058A: 0.2 ml

#E6058AA: 1.0 ml

1X NEBNext Quick Ligation Reaction Buffer:

66 mM Tris-HCl

10 mM MgCl₂

1 mM dithiothreitol

1 mM ATP

6% Polyethylene glycol (PEG 6000)

pH 7.6 @ 25 Degrees C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Nested mobile genetic elements mediating antimicrobial resistance genes mobility within and between Acinetobacter isolatesiScienceMay 15, 2026Kenneth Bongulto, Ngure Kagia, Hisamichi Tauchi, Satoru Suzuki, Kozo WatanabeNEBNext® Ultra II End repair/dA-tailing Module,New England BioLabs,E7546S NEBNext® Quick Ligation Module,New England BioLabs,E6056S Oxford Nanopore Technologies Ligation Sequencing Kit,Oxford Nanopore Technologies,SQK-LSK109 Flow

(2) Phage-assisted evolution of allosteric protein switchesNat CommunApril 14, 2026Nicholas T. Southern, Anna von Bachmann, Alisa Hovsepyan, Marielouise Griebl, Benedict Wolf, Nina Lemmen, Ann-Sophie Kroell, Simon Westermann, Jan Mathony, Dominik Niopekligated to sequencing adapters using the NEBNext Quick Ligation Module (New England Biolabs, cat. no. E6056S). Final libraries were purified using the Short Fragment Buffer provided in the ONT kit. Sequencing

(3) Pathogenic CGG expansions in oculopharyngodistal myopathy exhibit distinct characteristics of each causative gene on the flanking sequences as well as methylation statusGenome MedMarch 27, 2026Nobuyuki Eura, Satoru Noguchi, Megumu Ogawa, Kyuto Sonehara, Ai Yamanaka, Takashi Kurashige, Shinichiro Hayashi, Yukinori Okada, Kazuma Sugie, Ichizo NishinoAMII adapters were subsequently ligated to the barcoded genomic DNA using Quick T4 DNA ligase (NEB, #E6056S) at room temperature for 10 min, followed by purification on a magnet with AMPure XP Beads. The prepared

(4) Multi-omics analysis of associations between host demographics and saliva metabolome, sugar profiles, and microbiome profilesSci RepMarch 25, 2026Stefania Noerman, Anders Esberg, Carina I. Mack, Hany Ahmed, Björn Egert, Elise Nordin, Carl Brunius, Kati Hanhineva, Ingegerd Johansson, Rikard Landbergcleaning using 0.4x AMPure XP Beads, and finally fusing of the Native Adapter using T4 DNA Ligase (NEB, E6056). After additional cleaning and quantification, samples were loaded on a pre-primed R10.4.1 flow cell

(5) Optimizing CRISPR precision in mouse embryos via microhomology-mediated end joining-dominant targetingCommun BiolMarch 23, 2026Khanui Lkhagvadorj, Eiichi Okamura, Taito Taki, Hayate Suzuki, Akihiro Kuno, Yasushi Itoh, Seiya Mizuno, Knut Woltjen, Masatsugu EmaOxford Nanopore Technologies® Ligation Sequencing (NEB, E7180S) and NEBNext® Quick Ligation Module (NEB, E6056S) in accordance with the manufacturer’s instructions. The prepared library was loaded into the Flow Cell

(6) MASTR-seq enables multiplexed analysis of short tandem repeats with sequencingCell Rep MethodsMarch 23, 2026Chuanbin Su, Han-Seul Ryu, Keerthivasan Raanin Chandradoss, Thomas Malachowski, Ravi Boya, Linda Zhou, Hoa Emma Nguyen, Esteban O. Mazzoni, Kristen J. Brennand, Jennifer E. Phillips-Cremins(Thermo Fisher Scientific). We ligated adapters by first adding 20 μL NEBNext Quick Ligation Buffer (NEB#E6056S), 10 μL NEBNext Quick T4 DNA ligase (NEB#E6056S), and 5 μL Native Adapter (NA) at room temperature in

(7) MinION Sequencer Demonstrates Strong DNA Sequencing Accuracy and IdentityMicroPubl BiolMarch 22, 2026Andrea Gallagher, Pranav Illendula, Rongsun Pupreparation workflows, including NEBNext FFPE Repair Mix (cat# M6630), NEBNext Quick Ligation Module (cat# E6056), and NEBNext Ultra II End Repair/dA-tailing Module (cat# E7546) (Loman et al., 2015). When these reagents

(8) Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon PanelCurr ProtocFebruary 28, 2026Oluwaremilekun Grace Ajakaye, Michael E. GriggNEBNext Ultra II End Repair/dA‐Tailing module (NEB, E7546)NEB Blunt/TA Ligase master mix (NEB, M0367)NEBNext Quick Ligation module (NEB, E6056)200 fmol (130 ng for 1 kb amplicons) DNA per sample to be barcodedQubit dsDNA HS assay kit (Thermo Fisher,

(9) MinION Nanopore‐Enabled Identification and Genomic Characterization of Pseudomonas syringae Complex Infecting Blueberry Using Symptomatic Plant Samples and Pathogen Pure CultureCurr ProtocFebruary 25, 2026Rishi R. Burlakoti, Sanjib Sapkota, Pragyan Burlakoti, Mark Lubberts, Amy Novinscak, Harvinder BennypaulBarcoding Kit 24 V14 (Oxford Nanopore, cat. no. SQK‐NBD114.24)NEBNext Quick Ligation module (NEB, cat. no. E6056)50 mg/ml bovine serum albumin (BSA) (Invitrogen, cat. no. AM2616) PPE including laboratory coat, nitrile

(10) Antibody escape drives emergence of diverse spike haplotypes resembling variants of concern in persistent SARS-CoV-2 infectionsCell Reports MedicineFebruary 17, 2026Luke B. Snell, Suzanne Pickering, Adela Alcolea-Medina, Helena Winstone, Jeffrey Seow, Carl Graham, Lorcan O’Connell, Rahul Batra, Michael H. Malim, Katie J. Doores, et al.NEBNext® Ultra™ II End Repair/dA-Tailing Module,New England Biolabs,Cat #E7546S NEBNext® Quick Ligation Module,New England Biolabs,Cat#E6056S SARS-CoV-2 N gene standard,TIB MOLBIOL,Cat#30-7454-71 Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit,New England Biolabs

(11) Comparative gut microbiome analysis of Rohu fish from Halda River and Kaptai Lake using 16S rRNA sequencingScientific ReportsFebruary 13, 2026Mohammad Sharif Uddin, Kazi Chamonara, Maksudur Rahman Nayem, Afifa Siddiqua, Salma Chowdhury, Imam Hossain, A. S. M. Lutful Ahasan, Md. Habib Ullah MasumBarcoding Kit 24 V14 (SQK-NBD114.24) from Oxford Nanopore. The NEBNext® Quick Ligation Module (NEB, E6056) was used to ligate the sequencing adaptor, and the resultant DNA libraries were consolidated. The fully

(12) Integration of bioinformatic tools for the detection of SARS-CoV-2 co-infection casesMicrobial GenomicsJanuary 30, 2026Adeliza Mae L. Realingo, Francisco Gerardo M. Polotan, Miguel Francisco B. Abulencia, Roslind Anne R. Pantoni, Jessel Babe G. Capin, Gerald Ivan Sotelo, Maria Carmen A. Corpuz, Neil Tristan M. Yabut, Saul M. Rojas, Ma. Angelica Tujan, et al.cleaned up with 0.4X AMPure XP beads, and the adapters ligated using the NEBNext Quick Ligation Module (E6056, NEB). The final libraries were prepared using the ONT Ligation Sequencing Kit (SQK-LSK109) and loaded

(13) Efficient whole-genome sequencing of Monkeypox virus using a novel nuclease-multiple displacement amplification enrichment methodnpj VirusesJanuary 21, 2026Masayasu Misu, Takeshi Kurosu, Tomoki Yoshikawa, Madoka Kawahara, Kohei Oishi, Masayuki Shimojima, Hideki EbiharaAdapter (NA), 4 μL of 5× NEBNext Quick Ligation Buffer, and 2 μL of Quick T4 DNA Ligase (catalog No. E6056S; NEB). The mixture was incubated at room temperature for 20 min. Adapter-ligated DNA was purified using

(14) Expression of ultralong complementarity determining region 3 and development of IgM and IgG B cell receptor repertoires in Holstein heifer calvesImmunoHorizonsJanuary 14, 2026Tess E Altvater-Hughes, Harold P Hodgins, Douglas C Hodgins, Cathy A Bauman, Bonnie A Mallardwater. Adapters were added using the NEBNext Quick Ligation Module (New England Biolabs, catalog no. E6056S) and the ONT Ligation Sequencing Kit V14 (SQK-LSK114), and then samples were washed and eluted in nuclease-free

(15) Bacterial chromatin remodeling associated with transcription-induced domains at pathogenicity IslandsNature CommunicationsJanuary 8, 2026Mounia Kortebi, Mickaël Bourge, Romain Le Bars, Erwin Van Dijk, Charles J. Dorman, Stéphanie Bury-Moné, Frédéric Boccard, Virginia S. LioyIllumina sequencing were ligated using the NEBNext Quick Ligation Module (New England Biolabs, Cat# E6056S) for 15 min at RT. The ligation reaction was then purified using AMPure XP beads. First, the sample

(16) Oral microbiota in an aging Swedish population with high dental disease burden: an observational registry-based studyFrontiers in Oral HealthJanuary 6, 2026Anders Esberg, Simon Haworth, Ingegerd Johanssonpooled and cleaned using 0.4× AMPure XP Beads, and a Native Adapter was fused using T4 DNA Ligase (NEB, E6056). Following additional cleaning with 0.4× AMPure XP Beads (Beckman Coulter), libraries were quantified

(17) Impact of bovine lactoferrin supplementation and reduced iron in formula on infant oral microbiome: a randomized controlled trialJournal of Oral MicrobiologyDecember 31, 2025Cynthia Anticona, Anders Esberg, Staffan K. Berglund, Maria Björmsjö, Olle Hernell, Bo Lönnerdal, Ingegerd Johansson, Pernilla Lif HolgersonThe purified and pooled samples were finally fused to the Native Adapter using T4 DNA Ligase (NEB, E6056) and finally cleaned with 0.4x AMPure XP Beads (Beckman Coulter, Brea, CA). Libraries were quantified

(18) Gut and oral microbiome profiles in patients with obesity and ischemic heart diseaseFront Cell Infect MicrobiolDecember 12, 2025Antonina Starodubova, Georgy Leonov, Nataliya Shaposhnikova, Yurgita Varaeva, Elena Livantsova, Denis Fotin, Tatiana Kirichenko, Mariam Bagheri Ekta, Yuliya Markina, Stanislav Koshechkin, et al.“NEBNext End repair / dA-tailing Module” (E7546), and adapter ligation “NEBNext Quick Ligation Module” (E6056). All enzyme-dependent (intermediate) stages of library preparation were accompanied by the necessary

(19) Genome-Wide Identification of Tissue-Specific Fitness Genes in Murine Models of Staphylococcus aureus InfectioniScienceNovember 28, 2025Sally W. Yousief, Nader Abdelmalek, Martin S. Bojer, Yibing Ma, Priscila R. Guerra, Sajid Nisar, John E. Olsen, Bianca PagliettiNEBNext dA-Tailing Module,New England Biolab,Cat# E6053 NEBNext Quick Ligation Module,New England Biolab,Cat# E6056 KAPA HiFi HotStart ReadyMix,KAPA Biosystems (Roche),Cat# KK2601 "KAPA Library Quantification Kit (Illumina,

(20) Metagenomics of the MAST-3 stramenopile, Incisomonas, and its associated microbiome reveals unexpected metabolic attributes and extensive nutrient dependenciesMicrobial GenomicsNovember 26, 2025Dominic E. Absolon, Victoria L. N. Jackson, Adam Monier, Alison G. Smith, Katherine E. HelliwellNew England Biolabs), NEBNext Ultra II end repair/dA-tailing module (E7546; NEB) and NEBNext quick ligation module (E6056; NEB), following the ONT protocol (version GDE_9063_V109_REVV_14AUG2019) with a modified incubation

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