Quick LigationKit from New England Biolabs

Product Specs
ItemQuick LigationKit
CompanyNew England Biolabs
Price $413.00 Inquire
Catalog NumberM2200L
Time5 minutes
Quantity150 reactions
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

Specifications/Features

Information:
Ligation performed at room temperature; suitable for all common ligation reactions.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in miceMetabolismMay 1, 2026Jisun So, Jamie Wann, Kyungchan Kim, Solaema Taleb, Hyeong-Geug Kim, Manju Kumari, Alexander S. Banks, X. Charlie Dong, Hyun Cheol Rohfollowed by A-tailing with Klenow Fragment (NEB, M0212) and adaptor ligation with Quick Ligase (NEB, M2200). Libraries were then PCR-amplified for 18 cycles with PfuUltra II Hotstart PCR Master Mix (Agilent,

(2) Technical and biological sources of noise confound multiplexed enhancer AAV screeningNat CommunApril 28, 2026Avery C. Hunker, John K. Mich, Naz Taskin, Amy Torkelson, Trangthanh Cardenas, Jean-Benoît Lalanne, Joseph T. Mahoney, Darren Bertagnolli, Anish Bhaswanth Chakka, Rushil Chakrabarty, et al.then subcloned into the digested AiP11839 using a 2:1 insert:backbone ratio with T4 Quick Ligase (NEB M2200L) for 10 minutes at room temperature. The reactions were then transformed into chemically competent Stbl3

(3) PEX11 mediates intralumenal vesicle formation in peroxisomesNat CommunApril 21, 2026Nathan E. Tharp, Chelsea An, James Hwang, Nayeli S. Shad, Zachary J. Wright, Bonnie BartelThe resultant plasmids were digested with XhoI and religated with Quick Ligase (New England Biolabs, M2200) to remove 35S promoter and then digested with AvrII and NheI and religated to remove pACT2-tdTomato-PTS1

(4) Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunityNat CommunApril 20, 2026Kotaro Chihara, Aa Haeruman Azam, Artyom A. Egorov, Ilya Terenin, Masanori Hashino, Koichi Watashi, Kazuhiro Horiba, Vasili Hauryliuk, Kotaro Kigaend-prepped DNA, annealed Adapter 1, T4 DNA ligase buffer (NEB, #B0202S), and NEB Quick Ligase (NEB, #M2200S) were incubated at 20 °C for 30 min and then at 16 °C overnight. Ligation products were purified using

(5) PALINCODE: Recording cell lineage with ternary palindromic CRISPR bitsbioRxivApril 19, 2026Maryam Fathi, Aidan Cook, Bagheri Bagheri, Tyler Curiel, Aaron McKennaenzymes, NEBuilder HiFi DNA Assembly Master Mix (#E2621) for Gibson assembly, and the Quick Ligation Kit (#M2200) were used, all sourced from New England Biolabs (NEB). Q5® High-Fidelity 2X Master Mix (#M0492) from

(6) A Novel Mechanism for Depot-Specific Leptin Gene Expression Regulation and its Persistence After Weight LossiScienceApril 17, 2026Natalie Taege, Jan Hendric Britsemmer, Andreas Israel, Christin Krause, Sina Junge, Annette Feuchtinger, Siegfried Ussar, Paul Thomas Pfluger, Timo Gemoll, Sonja Charlotte Schriever, et al.fluoride membrane,"Merck Millipore, Ireland",IPVH00010 Quick ligase,"New England Biolabs, US",NEB #M2200 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, DUAL-GLO

(7) Dissecting the homeodomain MAT locus and engineering novel tripolar and bipolar mating systems in Cryptococcus amylolentusmBioApril 8, 2026Liping Xiong, Vikas Yadav, Sheng Sun, Joseph Heitmanreplacing the previous GPD1 promoter and the GPD1 terminator, using the NEB Quick Ligation Kit (NEB M2200S). The resulting plasmid pLX1-Cas9 served as the template to amplify the whole C. amylolentus Cas9 expression

(8) RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1Nucleic Acids ResMarch 17, 2026Parnia Babaei, Alice O Coomer, Kaliya Georgieva, Giulia Guiducci, Elisa Vitiello, Martin Dodel, Eleni Maniati, Hanya Elsayed Eid, Anisha Thind, Sneha Krishnamurthy, et al.704, QIAGEN). Annealed oligonucleotides were ligated into linearized pxR003 with Quick Ligation Kit (M2200S, NEB) for 20 min at RT. NEB5α (C2987H, NEB) cells were transformed with the ligated pxR003 vector according

(9) RNA-seq at different stages of human pancreatic β cell differentiation reveals proliferation dynamics and SMAD9 in directing β cell fateCell Death & DiseaseMarch 10, 2026Euodia Xi Hui Lim, Gabriel Jing Xiang Ong, Daniel Aron Ang, Adrian Kee Keong Teocut with Age1 (NEB #R3552).and EcoRI (NEB #R3101) with the Quick Ligation™ Kit (New England Biolabs, M2200L). Successful ligation was confirmed with Sanger sequencing and plasmids were amplified for further experimental

(10) Massively parallel reporter assay for mapping gene-specific regulatory regions at single-nucleotide resolutioneLifeFebruary 25, 2026Alastair J Tulloch, Ryan Nicholas Delgado, Rinaldo Catta-Preta, Constance L Cepkogel-purified. 500 ng of purified fragment was circularized in a 100 µl Quick Ligation reaction (NEB, cat. #M2200) at RT for 5 min. The ligation product was column-purified and treated with 50 µl exonuclease (Lucigen,

(11) Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cellsScientific ReportsFebruary 20, 2026Nicole M. Jenkinson, Harsh Oza, Kevin J. Yarema, Kristine GlundeCleanup kit (Qiagen #28506). Ligation was performed with NEB Quick Ligation kit (New England Biolabs #M2200S) and plasmids were transformed into NEB Stable Competent E. coli (New England Biolabs #C3040H) using

(12) ACTG1 facilitates proliferation and epithelial-mesenchymal transition-mediated metastasis via COL21A1 in gastric canceriScienceFebruary 20, 2026Shoumin Li, Bingxue Lan, Li Pan, Ming Sun, Miaomiao Cui, Yun Huang, Sixi Wei, Hai HuangBuffer,Fermentas,Cat#B64 lentiCas9-Blast,Addgene,Cat#52962 lentiGuide-Puro,Addgene,Cat#52963 Quick Ligase Buffer,NEB,Cat#B6058S Quick Ligase,NEB,Cat#M2200S T4 Ligation Buffer,NEB,Cat#B0202S T4 PNK,NEB,Cat#M0201S T4 DNA Ligase,NEB,Cat#M0202 ,, Deposited

(13) Integration of ploidy, Orc1/Cdc6 homolog function, and lysine acetylation with phosphate limitation and UV stress responses of Haloferax volcaniiExtremophilesFebruary 10, 2026Daniel Gal, Colton Smith, Kelsey Cooper, Ricardo L. Couto-Rodríguez, Julie A. Maupin-FurlowT1120L). The insert and vector DNA fragments were then ligated using the Quick Ligation Kit (NEB, cat. no. M2200S). The DNA ligation products were used to transform pre-competent E. coli Top10 cells. Transformants

(14) The role of cohesin loading at enhancers in the flux of loop extrusion and long-range transcriptional controlbioRxivFebruary 7, 2026Erika C. Anderson, Hadi Rahmaninejad, Abrar Aljahani, Emily M. Arnold, Annie S. Adachi, Rini Shah, Karissa L. Hansen, Ivana Cavka, Alistair N. Boettiger, Geoffrey Fudenberg, et al.Gibson assembly (NEB HiFi DNA Assembly Master Mix E2621) or restriction-ligation (NEB Quick Ligase M2200). A list of all plasmids used in this study is available in Supplementary Table 1 and 2. Cell culture Parental

(15) TP53/TAU axis regulates microtubule bundling to control alveolar stem cell mediated regenerationJournal of Clinical InvestigationFebruary 5, 2026Satoshi Konishi, Khaliun Enkhbayar, Shuyu Liu, Naoya Miyashita, Yoshihiko Kobayashi, Vera Hutchison, Ashna Sai, Pankaj Agarwal, Jonathan Witonsky, Nathan D. Jackson, et al.from the gel. Finally, the annealed oligo was ligated to backbone plasmid using Quick ligase (NEB, M2200S). AAV production and transduction. AAV production and transduction were performed as previously described

(16) Regulatory grammar in human promoters uncovered by MPRA-based deep learningNatureFebruary 4, 2026Lucía Barbadilla-Martínez, Noud Klaassen, Vinícius H. Franceschini-Santos, Jérémie Breda, Hatice Yücel, Miguel Hernández-Quiles, Tijs van Lieshout, Carlos G. Urzua Traslaviña, Minh Chau Luong Boi, Maryam Akbarzadeh, et al.ends of the fragments were ligated to the fragments using TA-ligation with a Quick Ligation kit (NEB, M2200L; see Supplementary Table 1 for oligonucleotide sequences). These adapters contain overlaps with the

(17) Unraveling the intra-species genomic diversity of sweetpotato-infecting CRESS-DNA and RNA viruses in Burkina Faso using Oxford Nanopore sequencingFrontiers in MicrobiologyFebruary 4, 2026Pakyendou E. Name, Ezechiel B. Tibiri, Fidèle Tiendrébéogo, Seydou Sawadogo, Florencia Djigma, Lassina Traoré, Angela O. Eni, Justin S. Pita(New England Biolabs, MA, USA, Cat. No. B6058S) and Quick T4 DNA Ligase (New England Biolabs, MA, USA, Cat. No. M2200S). The final libraries were loaded onto two Flow cells R10.4.1 (FLO-MIN114) and one Flongle Flow cell

(18) KRASG12D mutation promotes pancreatic tumorigenesis by suppressing sirtuin three via the guanine nucleotide exchange factor RCC1Journal of Biological ChemistryFebruary 1, 2026Taoyi Mai, Mengwen Wang, Ya Qiu, Wenhua Lu, Hongyu Wu, Shuna Chen, Paul J. Chiao, Peng Huangcell transfection The shRNA of SIRT3 and RCC1 were constructed into pLKO.1 vector backbone using Quick Ligation Kit (M2200L, New England Biolabs). Coding sequence of SIRT3 was constructed into pLVX vector backbone, and coding

(19) Neural stem cell quiescence is actively maintained by the epigenomeCell ReportsJanuary 27, 2026Anna Malkowska, Jan Ander, Andrea H. BrandT4 DNA Ligase,NEB,M0202S T4 DNA polymerase (3 U/μL),NEB,M0203S T4 polynucleotide kinase,NEB,M0201S Quick ligase,NEB,M2200S 1MTris pH 8.0,–,– SpeedBead Magnetic Carboxylate,Cytiva,65152105050250 100% ethanol,–,– Deoxynucleotide (dNTP) Solution Set

(20) Genome-wide regulon of NtrC reveals genetic regulation under nitrogen limitation in Methylomonas sp. DH-1iScienceJanuary 16, 2026Sukjae Han, Joon Young Park, Yong Hee Han, Ina Bang, Hyeonwoo Mun, Ok Kyung Lee, Ji Sung Hyung, Jeong-Geol Na, Eun Yeol Lee, Donghyuk Kim, et al.,New England Biolabs,Cat#E6050L NEBNext® dA-Tailing Module,New England Biolabs,Cat#E6053S Quick Ligation™ Kit,New England Biolabs,Cat#M2200S PreCR® Repair Mix,New England Biolabs,Cat#M0309S Lambda Exonuclease,New England Biolabs,Cat#M0262S RecJ

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(1)

4/14/2016Quick and Easy Ligation Kit Benjamin GerovacThis kit is used to ligate digested DNA for cloning. Primarily used to ligate sticky ends together and ligation is completed in 5 minutes.

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