Mo-DC Differentiation Medium, human from Miltenyi Biotec

(1) PARP1 trapping activates cGAS-STING pathway to induce immunogenic cell death in multiple myelomaCancer Cell InternationalJanuary 8, 2026Giada Juli, Domenica Ronchetti, Stefania Signorelli, Sara Squillacioti, Nicoletta Polerà, Anna Maienza, Michelangelo Vocaturo, Antonio Galvano, Elisa Taiana, Pierosandro Tagliaferri, et al.beads (Miltenyi Biotec), cultured for 6 days in Mo-DC differentiation medium containing GM-CSF and IL4 (130–094–812; Miltenyi Biotec). LP1 and KMS26 cells lines, were stained with CellTrace Far Red (Thermo Fisher Scientific),

(2) A BCMA-mRNA vaccine is a promising therapeutic for multiple myeloma∗BloodNovember 6, 2025Debasmita Dutta, Jiye Liu, Kenneth Wen, Arghya Ray, Alessandro Salatino, Xiangdong Liu, Annamaria Gulla, Teru Hideshima, Yan Song, Kenneth C. Andersondifferentiation medium, containing granulocyte-macrophage colony-stimulating factor and interleukin-4 (130-094-812; Miltenyi Biotec).22 The MoDCs were incubated with treatment groups: control, free cyanine-5 (Cy5)–tagged

(3) ATOR-4066, a Bispecific Antibody Targeting CD40 and CEACAM5, Induces Strong Myeloid and T Cell–Dependent Tumor Immunity and Synergizes with PD-1 BlockadeCancer Immunol ResSeptember 10, 2025Hampus Andersson, Ida Uddbäck, Tova Hermodsson, Mona Celander, Amulya Krishna Shetty, Lill Ljung, Anneli Nilsson, Anette Sundstedt, Laura von Schantz, Laura A. Varas, et al.differentiating the CD14+ monocytes for 1 week in Mo-DC Differentiation Medium supplemented with GM-CSF and IL-4 (130-094-812, Miltenyi Biotec). After 3 and 6 days, new media were added. On day 7, mature MoDCs were harvested for

(4) The tetravalent, bispecific properties of FS118, an anti-LAG-3/PD-L1 antibody, mediate LAG-3 shedding from CD4 + and CD8 + tumor-infiltrating lymphocytesAnti-Cancer DrugsJuly 1, 2025Claire S. Reader, Wenjia Liao, Beatrice J. Potter-Landua, Christel Séguy Veyssier, Claire J. Seal, Neil Brewis, Michelle Morrowdynabeads (1131D; Thermo Fisher Scientific), and monocytes were cultured in Mo-DC differentiation medium (130-094-812; Miltenyi Biotec) and differentiated into immature dendritic cells (iDC) for 7 days; 1 × 105 expanded

(5) The tetravalent, bispecific properties of FS118, an anti-LAG-3/PD-L1 antibody, mediate LAG-3 shedding from CD4+ and CD8+ tumor-infiltrating lymphocytesAnticancer DrugsMarch 3, 2025Claire S. Reader, Wenjia Liao, Beatrice J. Potter-Landua, Christel Séguy Veyssier, Claire J. Seal, Neil Brewis, Michelle Morrowdynabeads (1131D; Thermo Fisher Scientific), and monocytes were cultured in Mo-DC differentiation medium (130-094-812; Miltenyi Biotec) and differentiated into immature dendritic cells (iDC) for 7 days; 1 × 105 expanded

(6) A conserved pilin from uncultured gut bacterial clade TANB77 enhances cancer immunotherapyNature CommunicationsDecember 27, 2024Chan Yeong Kim, Dong Jin Park, Beung Chul Ahn, Seungbyn Baek, Min Hee Hong, Linh Thanh Nguyen, Sun Ha Hwang, Nayeon Kim, Daniel Podlesny, Askarbek Orakov, et al.cells were seeded in non-tissue culture-treated 24-well plates in 1 mL of Mo-DC differentiation medium (130-094-812, Miltenyi Biotec). On the second day of culture, 1 mL of fresh Mo-DC differentiation medium was added.

(7) BCMA-targeted bortezomib nanotherapy improves therapeutic efficacy, overcomes resistance, and modulates the immune microenvironment in multiple myelomaBlood Cancer JDecember 11, 2023Debasmita Dutta, Jiye Liu, Kenneth Wen, Keiji Kurata, Mariateresa Fulciniti, Annamaria Gulla, Teru Hideshima, Kenneth C. Andersonisolated monocytes were cultured for 6 days with Mo-DC differentiation medium containing GMCSF and IL4 (130-094-812; Miltenyi Biotec) to develop dendritic cells. Naive T cells were isolated using the Pan T Cell Isolation

(8) Systems serology-based comparison of antibody effector functions induced by adjuvanted vaccines to guide vaccine designNPJ vaccinesMarch 8, 2023Loos C, Coccia M, Didierlaurent AM, Essaghir A, Fallon JK, Lauffenburger D, Luedemann C, Michell A, van der Most R, Zhu AL, et al.differentiation medium containing granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (Miltenyi, #130-094-812). Red fluorescent 1-μm beads (Thermo Fisher, #F8775) were coupled to HBsAg, blocked with PBSA, and then

(9) The New Microtubule-Targeting Agent SIX2G Induces Immunogenic Cell Death in Multiple MyelomaInternational journal of molecular sciencesSeptember 6, 2022Grillone K, Riillo C, Rocca R, Ascrizzi S, Spanò V, Scionti F, Polerà N, Maruca A, Barreca M, Juli G, et al.manufacturer instructions. CD14+ cells were seeded in RPMI-1640 medium containing GM-CSF and IL-4 (130-094-812; Miltenyi Biotec, Bergisch Gladbach, Germany) in 5% CO2 at 37 °C. 4.5. Cell Viability, Apoptosis and

(10) Bortezomib induces anti-multiple myeloma immune response mediated by cGAS/STING pathway activationBlood cancer discoverySeptember 1, 2021Gulla A, Morelli E, Samur MK, Botta C, Hideshima T, Bianchi G, Fulciniti M, Malvestiti S, Prabhala RH, Talluri S, et al.(Miltenyi Biotec), cultured for 6 days in mo-DC differentiation medium containing GM-CSF and IL-4 (130-094-812; Miltenyi Biotec). AMO1 and NCI-H929 cells lines, as well as patient MM cells, were stained with CellTrace™

(11) Japanese Encephalitis Virus Infected Human Monocyte-Derived Dendritic Cells Activate a Transcriptional Network Leading to an Antiviral Inflammatory ResponseFrontiers in ImmunologyJune 17, 2021Shailendra Chauhan, Deepak Kumar Rathore, Shilpa Sachan, Sebastien Lacroix-Desmazes, Nimesh Gupta, Amit Awasthi, Sudhanshu Vrati, Manjula Kalia(Figure S1). The cells were differentiated in vitro, by incubating in Mo-DC differentiation medium (130-094-812, Miltenyi Biotec), and by day 5 immature DCs were obtained (CD14- CD209+). The quality of the prep was

(12) Chemoradiation induces upregulation of immunogenic cell death-related molecules together with increased expression of PD-L1 and galectin-9 in gastric cancerScientific reportsJune 10, 2021Petersen SH, Kua LF, Nakajima S, Yong WP, Kono K.130050201). Purified monocytes were cultured for 7 days in Mo-DC Differentiation Medium (Miltenyi Biotec, 130094812) to generate immature Mo-DCs. Immature Mo-DCs were cultured in Mo-DC Maturation Medium (Miltenyi Biotec,

(13) Genomic Multiple Sclerosis Risk Variants Modulate the Expression of the ANKRD55-IL6ST Gene Region in Immature Dendritic CellsFrontiers in immunologyJanuary 1, 2021Mena J, Alloza I, Tulloch Navarro R, Aldekoa A, Díez García J, Villanueva Etxebarria A, Lindskog C, Antigüedad A, Boyero S, Mendibe-Bilbao MDM, et al.density of 106 cells/ml in moDC differentiation medium (containing IL-4/GM-CSF; Miltenyi Biotec, Ref. 130-094-812) for 6 days, with fresh medium added on day 3. Maturation of moDCs was induced using combinations of

(14) The biologically functional identification of a novel TIM3-binding peptide P26 in vitro and in vivoCancer chemotherapy and pharmacologyDecember 1, 2020Zhong T, Zhao C, Wang S, Tao D, Ma S, Shou C.plus 10% FBS (Gbico) and hygromycin B (100 μg/mL) at 37 ℃, 5% C O2. Mo-DC Differentiation Medium (130-094-812) and Mo-DC Maturation Medium (130-094-813) were purchased from Miltenyi Biotech. Western blot analysis Cells

(15) Pharmacological Targeting of the ER-Resident Chaperones GRP94 or Cyclophilin B Induces Secretion of IL-22 Binding Protein Isoform-1 (IL-22BPi1)International journal of molecular sciencesMay 17, 2019Gómez-Fernández P, Urtasun A, Astobiza I, Mena J, Alloza I, Vandenbroeck K.Germany) following manufacturer’s instruction. Monocytes were cultured in Mo-DC differentiation medium (130-094-812, Miltenyi) at a density of 1 × 106 cells/mL for three days; at this point, an equal volume of fresh

(16) Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein ResponseFrontiers in immunologyJanuary 1, 2018Gómez-Fernández P, Urtasun A, Paton AW, Paton JC, Borrego F, Dersh D, Argon Y, Alloza I, Vandenbroeck K.differentiation medium based on RPMI 1640 medium containing FBS, L-glutamine, IL-4 and GM-CSF (Miltenyi, 130-094-812), for 6 days, or as indicated, and an equal volume of fresh Mo-DC medium was added on day 3. Maturation

(17) The Contact Allergen NiSO₄ Triggers a Distinct Molecular Response in Primary Human Dendritic Cells Compared to Bacterial LPSFrontiers in immunologyJanuary 1, 0001"Höper T, Siewert K, Dumit VI, von Bergen M, Schubert K, Haase A."ready-to-use MoDC differentiation medium containing 400 IU/ml IL-4 and 500 IU/ml GM-CSF (Miltenyi Biotec, 130-094-812) at 37°C with 5% CO2. Every two days, half of the culture medium was replaced with fresh medium. On

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Mo-DC Differentiation Medium, human from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Mo-DC Differentiation Medium, human from Miltenyi Biotec

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