DdeI from New England Biolabs

Product Specs
ItemDdeI
CompanyNew England Biolabs
Price $308.00 Inquire
Catalog NumberR0175L
Quantity5000 units
Concentration10 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/TNAG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Integrative spatial profiling of 3D genome organization and gene expression in tissueCellAugust 25, 2026Pengfei Guo, Yan Cui, Jincan He, Abraham J. Waldman, Jiaxin Zhu, Yufan Chen, Zhi Huang, Jingtian Zhou, Jennifer E. Phillips-Cremins, Yanxiang Deng,New England Biolabs,B7202S rCutSmart™ Buffer,New England Biolabs,B6004S DpnII,New England Biolabs,R0543S DdeI,New England Biolabs,R0175S HinfI,New England Biolabs,R0155S DPBS,Thermo Fisher Scientific,14190144 Proteinase K,Thermo Fisher Scientific

(2) A reproducible three-dimensional model of human brain tissue to investigate physiological and disease-associated microglia phenotypesNature NeuroscienceAugust 4, 2026Julien Klimmt, Carolina Cardoso Gonçalves, Jessica Valentina Montgomery, Stephan A. Müller, Merle Bublitz, Severin Filser, Lars Paeger, Brigitte Nuscher, Angelika Dannert, Sigrun Roeber, et al.using the enzymes HpyCH4IV (cat. no. R0619S, New England Biolabs) for APP Arctic, and DdeI (cat. no. R0175S, New England Biolabs) for APP Iberian, followed by Sanger sequencing as described elsewhere104. Successfully

(3) Dose-dependent sensitivity of human three-dimensional chromatin to a heart disease–linked transcription factorScienceJuly 23, 2026Zoe L. Grant, Shuzhen Kuang, Shu Zhang, Abraham J. Horrillo, Zhe Chen, Kavitha S. Rao, Cemre Celen, Vasumathi Kameswaran, Carine Joubran, Pik Ki Lau, et al.DNA was digested using 50 U DpnII (New England Biolabs #R0543M) and 50 U DdeI (New England Biolabs #R0175L) restriction enzymes in 1x NEB Buffer 3.1 for 60 min at 37°C. All samples passed Arima-QC1 Quality Control.

(4) First genome-wide centromere map of Trypanosoma cruzi suggests linear and 3D compartment boundaries and spatial clusteringBMC GenomicsJune 17, 2026Natália Karla Bellini, Pedro Gabriel Nachtigall, Pedro Leonardo Carvalho de Lima, Herbert Guimarães de Sousa Silva, David da Silva Pires, Vinicius Carius de Souza, Claudia Rabuffo, Danielle Bruno de Carvalho, Yasmin Morais Feno, Ana Paula Cabral de Araujo Lima, et al.min, at room temperature. Chromatin was digested with 100 U MboI (NEB, R0147M) and 100 U DdeI (NEB, R0175L), overnight at 37 ºC, 400 rpm shaking. Reaction was stopped by incubation at 65ºC for 20 min, followed

(5) Splice-switching ASOs targeting the AURKA 5′ UTR collapse an SRSF1-AURKA-MYC oncogenic circuit in pancreatic cancerMolecular CellJanuary 8, 2026Alexander J. Kral, Lu Jia, GeunYoung Sim, Ledong Wan, Yuma Ishigami, Adrian R. Krainer87786 HindIII-HF,NEB,Cat# R3104S NcoI-HF,NEB,Cat# R3193S EcoR1-HF,NEB,Cat# R3101S AgeI-HF,NEB,Cat# R3552S DdeI,NEB,Cat# R0175S KpnI-HF,NEB,Cat# R3142S NcoI-HF,NEB,Cat# R3193S Polybrene,Sigma,Cat# TR-1003-G NEBuffer 2,NEB,Cat#

(6) Pre-established ATF4 occupancy and chromatin organization instruct selective transcription activation during integrated stress responseNat CommunOctober 28, 2025Peipei Jiang, Qian Bianovernight at 37 °C by adding 50 μL of 10× NEB Buffer 2400 U of DpnII (NEB #R0543M), and 400 U of DdeI (NEB #R0175L). After digestion, restriction enzymes were inactivated by incubating at 62 °C for 20 min. To fill in

(7) Cited4a limits cardiomyocyte dedifferentiation and proliferation during zebrafish heart regenerationDevelopmentOctober 15, 2025Rachel Forman-Rubinsky, Angela Paul, Wei Feng, Brent T. Schlegel, Daniel A. Zuppo, Katarzyna Kedziora, Donna B. Stoltz, Simon C. Watkins, Dhivyaa Rajasundaram, Guang Li, et al.assay (Botstein et al., 1980), and the PCR product was digested using restriction enzyme DdeI (NEB, R0175S). Two independent alleles were established: pt38a (95bp deletion) and pt38b (19bp deletion). Both alleles

(8) Mutual Antagonism Between PRC1 Condensates and SWI/SNF in Chromatin RegulationbioRxivAugust 30, 2025Stefan Niekamp, Sharon K. Marr, Rebecca Sanon, Philipp C. Schneider, Radhika Subramanian, Robert E. Kingston0.2 ml of BsmBI_v2 (NEB, R0739L), then incubated at 55 °C for 48 hours. Next, 0.2 ml of DdeI (NEB, R0175L) was added and the reaction was incubated at 37 °C for an additional 24 hours. Completion of digestion

(9) Brucella infection induces chromatin restructuring in host cells to activate immune responsesFront ImmunolJune 5, 2025Dejian Xie, Heling Xu, Changwei Su, Jingjing Lu, Wenlong Shen, Ping Li, Bingyu Ye, Jiabao Hou, Junwei Deng, Yan Zhang, et al.at 4°C to remove the supernatant. The genome was digested with 200U DdeI (New England Biolabs, Cat# R0175L) and 200U DpnII restriction enzymes (New England Biolabs, Cat# R0543M) for 16 hours. The digestion was

(10) DNA G-quadruplex structures act as functional elements in α- and β-globin enhancersGenome BiolJune 4, 2025Colm Doyle, Krzysztof Herka, Sean M. Flynn, Larry Melidis, Somdutta Dhir, Stefan Schoenfelder, David Tannahill, Shankar Balasubramanianof 1 × TE buffer. For the second restriction enzyme digestion, DNA was incubated with 500 U of DdeI (R0175L, New England Biolabs) in 1 × NEBuffer r3.1 in 500 mL total volume overnight at 37 °C at 500 rpm. Enzyme

(11) Phenotype and genotype analyses of 21 Chinese patients with Dent diseaseThe Journal of Biomedical ResearchMay 27, 2025Ruochen Che, Yuwen Cai, Wei Zhou, Sanlong Zhao, Songming Huangthe template contained DNA digested with the methylation-sensitive restriction enzyme HpaII (Cat. #R0175L, New England Biolabs), whereas the other reaction contained undigested genomic DNA. PCR products were

(12) Phenotype and genotype analyses of 21 Chinese patients with Dent diseaseJ Biomed ResMay 27, 2025Ruochen Che, Yuwen Cai, Wei Zhou, Sanlong Zhao, Songming Huangthe template contained DNA digested with the methylation-sensitive restriction enzyme HpaII (Cat. #R0175L, New England Biolabs), whereas the other reaction contained undigested genomic DNA. PCR products were

(13) The Distribution of Sport Performance Gene Variations Through COVID-19 Disease SeverityDiagnostics (Basel)March 12, 2025Guven Yenmis, Ilayda Kallenci, Mehmet Dokur, Suna Koc, Sila Basak Yalinkilic, Evren Atak, Mahmut Demirbilek, Hulya Arkanas a single band of 460 bp in length (Figure 1b). One unit of the Ddel restriction enzyme (Catalog #R0175L, New England Biolabs, Ipswich, USA) was used to digest the ACTN3 PCR product. The PCR product and restriction

(14) Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3’ processingThe EMBO JournalFebruary 24, 2025Geng-Jen Jang, Alex L Payne-Dwyer, Robert Maple, Zhe Wu, Fuquan Liu, Sergio G Lopez, Yanning Wang, Xiaofeng Fang, Mark C Leake, Caroline Dean,Thermo Fisher Scientific,T7280 proteinase K,Thermo Fisher Scientific,AM2546 DdeI,New England Biolabs,R0111L NdeI,New England Biolabs,R0175 XbaI,New England Biolabs,R0145L RNAse III,Thermo Fisher Scientific,AM2290 Dynabeads Protein G for Immunoprecipitation

(15) Dose-dependent sensitivity of human 3D chromatin to a heart disease-linked transcription factorbioRxivJanuary 12, 2025Zoe L. Grant, Shuzhen Kuang, Shu Zhang, Abraham J. Horrillo, Kavitha S. Rao, Vasumathi Kameswaran, Carine Joubran, Pik Ki Lau, Keyi Dong, Bing Yang, et al.DNA was digested using 50 U DpnII (New England Biolabs #R0543M) and 50 U DdeI (New England Biolabs #R0175L) restriction enzymes in 1x NEB Buffer 3.1 for 60 min at 37°C. All samples passed Arima-QC1 Quality Control.

(16) Cited4a limits cardiomyocyte dedifferentiation and proliferation during zebrafish heart regenerationbioRxivDecember 12, 2024Rachel Forman-Rubinsky, Wei Feng, Brent T. Schlegel, Angela Paul, Daniel Zuppo, Katarzyna Kedziora, Donna Stoltz, Simon Watkins, Dhivyaa Rajasundaram, Guang Li, et al.Length Polymorphism assay [61], and the PCR product was digested using restriction enzyme DdeI (NEB, R0175S). Two independent alleles were established: pt38a (95bp deletion) and pt38b (19bp deletion). Both alleles

(17) Global loss of promoter–enhancer connectivity and rebalancing of gene expression during early colorectal cancer carcinogenesisNature CancerOctober 30, 2024Yizhou Zhu, Hayan Lee, Shannon White, Annika K. Weimer, Emma Monte, Aaron Horning, Stephanie A. Nevins, Edward D. Esplin, Kristina Paul, Gat Krieger, et al.20 μl of Cutsmart buffer (New England Biolabs (NEB)), 3 μl each of HinP1I (NEB, R0124S), DdeI (NEB, R0175L), CviAII (NEB, R0640L) and FspBI (ThermoFisher, ER1762) and 0.6 μl of MseI (NEB, R0525M) were added

(18) The small inhibitor WM-1119 effectively targets KAT6A-rearranged AML, but not KMT2A-rearranged AML, despite shared KAT6 genetic dependencyJournal of Hematology & OncologyOctober 8, 2024Mathew Sheridan, Muhammad Ahmad Maqbool, Anne Largeot, Liam Clayfield, Jingru Xu, Natalia Moncaut, Robert Sellers, Jessica Whittle, Jerome Paggetti, Mudassar Iqbal, et al.TGACTCCTCCTGCATGTTGT) flanking the homology arms followed by DdeI restriction digestion (New England Biolabs, R0175S) and subsequent Sanger sequencing. The conditional Kat6A allele with exon 4 flanked by loxP sites (KAT6AFL)

(19) Genotyping single point mutations in rd1 and rd8 mice using melting curve analysis of qPCR fragmentsScientific ReportsAugust 28, 2024Melanie E. Schwämmle, Felicitas Bucher, Günther Schlunck, Gottfried Martinthe same conditions as described above. After PCR, 1 µl of RE buffer and 1 µl (10 units) of Dde I (R0175S, New England Biolabs, Frankfurt am Main, Germany) were added and the mixture was incubated at 37 °C

(20) TERT upstream promoter methylation regulates TERT expression and acts as a therapeutic target in TERT promoter mutation-negative thyroid cancerCancer Cell InternationalAugust 3, 2024Shiyong Li, Guanghui Hu, Yulu Chen, Ye Sang, Qin Tang, Rengyun LiuSimpleChIP Plus Sonication Chromatin IP Kit (#56383, Cell Signaling Technology) and incubated with DdeI enzyme (#R0175L, NEB) for 24 h at 37℃ in water bath. The digested products were suspended with 1X ChIP buffer and sonicated

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DdeI

DdeI from New England Biolabs

Sequence

New England Biolabs

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DdeI from New England Biolabs

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