DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(341) Polypharmacological Profiles Underlying the Antitumor Property of Salvia miltiorrhiza Root (Danshen) Interfering with NOX-Dependent Neutrophil Extracellular TrapsOxidative medicine and cellular longevityJanuary 1, 2018Tao L, Xu M, Dai X, Ni T, Li D, Jin F, Wang H, Tao L, Pan B, Woodgett JR, et al.1 : 200, Abcam, Cat number ab150113). Nuclei were visualized with DAPI (0.4 μg/mL in PBS, CST, Cat number 4083). The coverslips were mounted onto glass slides with Prolong Gold mounting agent (Molecular Probes,

(342) Trypanosoma cruzi Exploits Wnt Signaling Pathway to Promote Its Intracellular Replication in MacrophagesFrontiers in immunologyJanuary 1, 2018Volpini X, Ambrosio LF, Fozzatti L, Insfran C, Stempin CC, Cervi L, Motran CC.counterstaining, the cells were incubated with 4′,6-diamidino-2-phenylindone (Cell Signaling Technology, #4083) for 10 min, washed in 1× PBS, and the slides were set with FluorSave™ reagent (EMD Millipore). Expression

(343) Cytosolic Internalization of Anti-DNA Antibodies by Human Monocytes Induces Production of Pro-inflammatory Cytokines Independently of the Tripartite Motif-Containing 21 (TRIM21)-Mediated PathwayFrontiers in immunologyJanuary 1, 2018Park H, Kim M, Seo Y, Ham Y, Cho MY, Kwon MH.

(344) ACTB Loss-of-Function Mutations Result in a Pleiotropic Developmental DisorderAmerican journal of human geneticsDecember 7, 2017Cuvertino S, Stuart HM, Chandler KE, Roberts NA, Armstrong R, Bernardini L, Bhaskar S, Callewaert B, Clayton-Smith J, Davalillo CH, et al.Life Technologies) was applied for 1 hr at room temperature in the dark. Samples were stained with DAPI (4083S, Cell Signaling Technology) for 5 min. Representative pictures show marked difference in the morphology

(345) Tracking mesenchymal stem cell contributions to regeneration in an immunocompetent cartilage regeneration modelJCI insightOctober 19, 2017Zwolanek D, Satué M, Proell V, Godoy JR, Odörfer KI, Flicker M, Hoffmann SC, Rülicke T, Erben RG.(ab150169, Abcam) for 1 hour at room temperature and mounted using Mowiol 4-88 (MilliporeSigma). DAPI (4083S, Cell Signaling) was used for nuclear staining. COL2 staining within the defect was quantified as percent

(346) MicroRNA-93 Regulates Hypoxia-Induced Autophagy by Targeting ULK1Oxidative Medicine and Cellular LongevityOctober 3, 2017Wen Li et al.anti-ATG7 (Sigma, A2856), anti-Beclin1 (CST, 3738), anti-Tom20 (BD Biosciences, 612278), DAPI (CST, 4083), HRP Affinipure goat anti-mouse IgG (Earthox, E030110), and HRP Affinipure goat anti-rabbit IgG (Earthox,

(347) High accuracy label-free classification of single-cell kinetic states from holographic cytometry of human melanoma cellsScientific reportsSeptember 20, 2017Hejna M, Jorapur A, Song JS, Judson RL.

(348) Use of confocal laser endomicroscopy with a fluorescently labeled fatty acid to diagnose colorectal neoplasmsOncotargetAugust 29, 2017Feihong Deng et al.paraformaldehyde for 30 minutes, and the nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 4083, Cell Signaling Technology, Boston, MA, USA). Following fixation, cells were washed three times with

(349) A novel epigenetic AML1-ETO/THAP10/miR-383 mini-circuitry contributes to t(8;21) leukaemogenesisEMBO molecular medicineJuly 1, 2017Li Y, Ning Q, Shi J, Chen Y, Jiang M, Gao L, Huang W, Jing Y, Huang S, Liu A, et al.USA) diluted in PBA‐BSA. After three PBS washes, nuclei were counterstained with DAPI (0.2 μg/ml, #4083, Cell Signalling Technology, USA). Following extensive washing in PBS, samples were air‐dried and mounted

(350) Resistin promotes the abnormal Type I collagen phenotype of subchondral bone in obese patients with end stage hip osteoarthritisScientific ReportsJune 22, 2017Ashleigh M. Philp, Rebecca L. Collier, Liam M. Grover, Edward T. Davis, Simon W. Jonesand secondary antibody (α-Rabbit H+L, Alexa Fluor 555, Pierce, UK) for 1 h with the addition of DAPI (4083, CST, USA). Cells were washed before mounting with Prolong® Diamond Antifade Moutant (Thermofisher). Statistical

(351) Unraveling the expression of the oncogene YAP1, a Wnt/beta-catenin target, in adrenocortical tumors and its association with poor outcome in pediatric patientsOncotargetDecember 20, 2016Rafael H. Abduch et al.1:250; sc_2078, Santa Cruz Biotechnology). Nuclear staining was made using DAPi (dilution: 1:25000 #4083, Cell Signaling Technology) and slides were set with Fluoromount (Sigma-Aldrich). Fluorescence was acquired

(352) Inhibition of Poly-ADP-Ribosylation Fails to Increase Axonal Regeneration or Improve Functional Recovery after Adult Mammalian CNS InjuryeNeuroNovember 1, 2016Wang X, Sekine Y, Byrne AB, Cafferty WB, Hammarlund M, Strittmatter SM.Cell density was visualized using nuclear marker 4′,6-diamidino-2-phenylindole (DAPI; 0.1 μg/mL, cat. #4083; Cell Signaling Technology). Images were taken on a 10× objective in an automated high-throughput imager

(353) Self-organization of human embryonic stem cells on micropatternsNature ProtocolsNovember 1, 2016Alessia Deglincerti et al.017-000-121). Tween-20 (Sigma-Aldrich, cat. no. P1379-500ML). DAPI (Cell Signaling Technologies, cat. no. 4083S). Primary antibodies: AntigenCommercial informationDilutionPOU5F1BD Biosciences 6112031:400SOX2Cell

(354) Inhibiting poly(ADP-ribosylation) improves axon regenerationeLifeOctober 4, 2016Alexandra B Byrne, Rebecca D McWhirter, Yuichi Sekine, Stephen M Strittmatter, David M Miller, Marc Hammarlund#R415, Life Technologies). Cell density was visualized using nuclear marker DAPI (0.1 μg/mL, catalog #4083, Cell Signaling Technology). Images were taken on a 10X objective in an automated high-throughput imager

(355) HPV16 E6-E7 induces cancer stem-like cells phenotypes in esophageal squamous cell carcinoma through the activation of PI3K/Akt signaling pathway in vitro and in vivoOncotargetAugust 30, 2016Ruxing Xi et al.secondary antibody was incubated for 1h at room temperature after three times PBS washing and DAPI (#4083, CST, USA) was applied to counterstain nucleus. All the primary antibodies were the same with western blotting

(356) MicroRNA-17-92 cluster regulates pancreatic beta-cell proliferation and adaptationMolecular and Cellular EndocrinologyAugust 23, 2016Yaxi Chen, Li Tian, Shan Wan, Ying Xie, Xiang Chen, Xiao Ji, Qian Zhao, Chunyu Wang, Kun Zhang, Janet M. Hock, Haoming Tian, Xijie Yu

(357) Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient miceAmerican journal of physiology. Heart and circulatory physiologyAugust 1, 2016Li W, Jin D, Hata M, Takai S, Yamanishi K, Shen W, El-Darawish Y, Yamanishi H, Okamura H.(8558), lysosome-associated membrane protein 1 (Lamp1; C54H11, 3243), and 4,6-diamidino-2phenylindole (4083) were purchased from Cell Signaling Technology (Beverly, MA). Anti-claudin 5 and anti-CD31 Ab were purchased from

(358) Highly variable cancer subpopulations that exhibit enhanced transcriptome variability and metastatic fitnessNature communicationsMay 3, 2016Nguyen A, Yoshida M, Goodarzi H, Tavazoie SF.

(359) Therapeutic efficacy of apelin on transplanted mesenchymal stem cells in hindlimb ischemic mice via regulation of autophagyScientific ReportsApril 1, 2016Dong Liang et al.Santa Cruz, CA) for 1 h at 37 °C. Nuclei were counterstained with 49, 6-diamidino-2-phenylindole (DAPI, 4083S, Cell Signaling Technology). Slides were photographed by confocal microscope (FluoView-FV1000, Olympus,

(360) Defective Angiogenesis and Intraretinal Bleeding in Mouse Models With Disrupted Inner Retinal LaminationInvestigative ophthalmology & visual scienceApril 1, 2016Simmons AB, Merrill MM, Reed JC, Deans MR, Edwards MM, Fuerst PG.Alexa 568 (I21412, 1:200; Invitrogen, Grand Island, NY, USA), 4′,6-diamidino-2-phenylindole (DAPI) (4083, 1:50,000 from 1 mg/mL stock; Cell Signaling Technology), DRAQ5 (62251, 1:2000; Thermo Scientific, Waltham,

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DAPI

DAPI from Cell Signaling Technology

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DAPI from Cell Signaling Technology

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