DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(321) Fibrinogen Is a Specific Trigger for Cytolytic Eosinophil DegranulationJournal of immunology (Baltimore, Md. : 1950)January 15, 2020Coden ME, Loffredo LF, Walker MT, Jeong BM, Nam K, Bochner BS, Abdala-Valencia H, Berdnikovs S.in at a 1:200 dilution in 1x PBS for 15 minutes prior to assay. DAPI (Cell Signaling Technologies, 4083) staining was performed on fixed cells at 1 μg/mL in 1x PBS for 5 minutes. For TEM, samples were fixed

(322) Treatment-Induced Tumor Dormancy through YAP-Mediated Transcriptional Reprogramming of the Apoptotic PathwayCancer cellJanuary 13, 2020Kurppa KJ, Liu Y, To C, Zhang T, Fan M, Vajdi A, Knelson EH, Xie Y, Lim K, Cejas P, et al.minutes, and washed 3 times with PBS. The nuclei were counterstained with 1 μg/ml DAPI (Cell Signaling #4083). The coverslips were mounted using Immu-Mount reagent (Thermo Scientific). Images from H3K9Me3-stainings

(323) TRPC6 Attenuates Cortical Astrocytic Apoptosis and Inflammation in Cerebral Ischemic/Reperfusion InjuryFrontiers in cell and developmental biologyJanuary 1, 2020Liu L, Chen M, Lin K, Xiang X, Yang J, Zheng Y, Xiong X, Zhu S.h at room temperature. In addition, 4′,6-iamidino-2-phenylindole, dihydrochloride (DAPI; 1 μg/mL; #4083; Cell Signaling Technology) staining was used to visualize the nuclei. Images were acquired using a

(324) Inhibition of Ctsk modulates periodontitis with arthritis via downregulation of TLR9 and autophagyCell ProliferationJanuary 1, 2020Wei Wei, Jie Ren, Wuwei Yin, Handong Ding, Qiuyu Lu, Liangyu Tan, Shibing Deng, Jie Liu, Qin Yang, Jiajia Wang, et al.antibody according to the manufacturer's instructions. The sections of IF were then stained with DAPI (Cat#4083, Cell Signaling Technology) to identify the nuclei.30 All samples in vitro were fixed by 4% paraformaldehyde

(325) Dysfunction of estrogen-related receptor alpha-dependent hepatic VLDL secretion contributes to sex disparity in NAFLD/NASH developmentTheranosticsJanuary 1, 2020Yang M, Liu Q, Huang T, Tan W, Qu L, Chen T, Pan H, Chen L, Liu J, Wong CW, et al.(1μg/mL, Sigma, N3013) for 10 min at room temperature. Nuclei were stained using DAPI (Cell signaling, 4083) and mounted with prolong gold anti-fade reagent (Invitrogen, P10144) followed by washing in PBS for

(326) Inhibiting Caspase-12 Mediated Inflammasome Activation protects against Oxygen-Glucose Deprivation Injury in Primary AstrocytesInternational journal of medical sciencesJanuary 1, 2020Liu L, Chen M, Lin K, Xiang X, Zheng Y, Zhu S.SA00013-4) were added for 1 h. After three washes with PBS, 1 μg/ml DAPI (Cell Signaling Technology, #4083) solution was added to cover the cells for 5 min in the dark followed by three washes with PBS. Fluorescence

(327) MicroRNA-17-92 Regulates Beta-Cell Restoration After Streptozotocin TreatmentFrontiers in endocrinologyJanuary 1, 2020Wan S, Zhang J, Chen X, Lang J, Li L, Chen F, Tian L, Meng Y, Yu X.anti-insulin and anti-BrdU (cat# sc-32323, Santa Cruz) antibodies then counterstained with DAPI (cat# 4083, Cell Signaling Technology) (38). The TUNEL assay kit (cat# G3250, Promega) was used to detect the apoptosis

(328) Naringin Protects Against Interleukin 1β (IL-1β)-Induced Human Nucleus Pulposus Cells Degeneration via Downregulation Nuclear Factor kappa B (NF-κB) Pathway and p53 ExpressionMedical Science MonitorDecember 25, 2019Gang Gao, Feng Chang, Ting Zhang, Xinhu Huang, Chen Yu, Zhaolin Hu, Mingming Ji, Yufen Duan(#7074, 1: 2000, CST, USA) for 1 hour at 37°C the next day. The NP cells nuclei were stained by DAPI (#4083, CST, USA) and the images were observed and captured by a fluorescence microscope (Nikon, Japan). Statistical

(329) miRNA-384-5p regulates the progression of Parkinson's disease by targeting SIRT1 in mice and SH-SY5Y cellsInternational Journal of Molecular MedicineDecember 12, 2019Hongli Tao, Yan Liu, Yingjuan Houwas incubated at room temperature for 2 h. Subsequently, the cells were incubated with DAPI (cat. no. 4083; 0.5 µg/ml) for 10 min at room temperature for cell nuclei staining. The above-mentioned antibodies

(330) Palmitate acid promotes gastric cancer metastasis via FABP5/SP1/UCA1 pathwayCancer Cell InternationalDecember 1, 2019Jiaomeng Pan, Qingqiang Dai, Tianqi Zhang, Chen LiSample sections were then incubated with Alexa Fluor 488 dye-conjugated secondary antibody and DAPI (Cat#4083S, CST). Pictures were taken using an Olympus BX50 microscope (Olympus, Tokyo, Japan). Statistical analysis All

(331) CD36 mediates palmitate acid-induced metastasis of gastric cancer via AKT/GSK-3β/β-catenin pathwayJournal of Experimental & Clinical Cancer ResearchDecember 1, 2019Jiaomeng Pan, Zhiyuan Fan, Zhenqiang Wang, Qingqiang Dai, Zhen Xiang, Fei Yuan, Min Yan, Zhenggang Zhu, Bingya Liu, Chen LiSample sections were then incubated with Alexa Fluor 594-conjugated secondary antibody and DAPI (Cat. # 4083S, CST). Photos were taken using an Olympus BX50 microscope (Olympus, Tokyo, Japan). Plasmid construction

(332) Glycogen branching enzyme controls cellular iron homeostasis via Iron Regulatory Protein 1 and mitoNEETNature communicationsNovember 29, 2019Huynh N, Ou Q, Cox P, Lill R, King-Jones K.secondary antibody dilution buffer for 1 h at RT, washed in 1× PBS3T and 1:50,000 DAPI (Cell Signaling #4083) for three times. Samples were mounted in Vectashield mounting medium (#VECTH1000). Pictures were taken

(333) Increased Tim-3 expression alleviates liver injury by regulating macrophage activation in MCD-induced NASH miceCellular & molecular immunologyNovember 1, 2019Du X, Wu Z, Xu Y, Liu Y, Liu W, Wang T, Li C, Zhang C, Yi F, Gao L, et al.Boston, USA), anti-CD68 (dilution: 1:100, mAb, ab955, Abcam, Southampton, UK), DAPI (dilution: 1:1000, #4083, CST), and Tyramide (TSA)conjugated ﬂuorophore (dilution: 1:100, NEL791001KT, PerkinElmer, T20950, Life Technologies

(334) Neuropeptide CGRP Limits Group 2 Innate Lymphoid Cell Responses and Constrains Type 2 InflammationImmunityOctober 15, 2019Nagashima H, Mahlakõiv T, Shih HY, Davis FP, Meylan F, Huang Y, Harrison OJ, Yao C, Mikami Y, Urban JF Jr, et al.50 cycles (paired-end reads) on a HiSeq 3000 (Illumina). Imagestream Cells stained for DAPI (CST, #4083), anti-NFAT1-AF488, anti-NF-kB p65-AF647, and anti-CD45-PE/Cy7 were run on an ImageStreamXMKII (Amnis,

(335) Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa CellsBiomoleculesSeptember 16, 2019Phattharachanok Khumkhrong et al.DyLight® 594 conjugated (ab96901; Abcam, Cambridge, UK), washed with PBS, and counterstained with DAPI (#4083; Cell Signaling Technology). Cell images were taken with a FV10i confocal laser scanning microscope

(336) A Systems Pharmacology Approach Uncovers Wogonoside as an Angiogenesis Inhibitor of Triple-Negative Breast Cancer by Targeting Hedgehog SignalingCell chemical biologyAugust 15, 2019Huang Y, Fang J, Lu W, Wang Z, Wang Q, Hou Y, Jiang X, Reizes O, Lathia J, Nussinov R, et al.Ltd. Thrombin,T4393,Sigma Fibrinogen,F4883,Sigma "4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)",#4083,Cell Signaling Technology Triton X-100,T8787,Sigma MG132,474791,EMD Millipore NH4Cl (Ammonium chloride),A9434,Sigma Dual Luciferase Reporter Assay Kit,DL101

(337) Intra-articularly injected mesenchymal stem cells promote cartilage regeneration, but do not permanently engraft in distant organsScientific reportsJuly 12, 2019Satué M, Schüler C, Ginner N, Erben RG.antibodies for 1 h at room temperature, air-dried, and mounted with Fluoromount (21644.01, Serva). DAPI (4083 S, Cell Signaling) was used to visualize nuclei. All immunofluorescence analyses were performed in a

(338) miR‑494‑3p regulates lipopolysaccharide‑induced inflammatory responses in RAW264.7 cells by targeting PTENMolecular medicine reportsMay 1, 2019Zhang S, He K, Zhou W, Cao J, Jin Z.Milbrae, CA, USA) for 1 h at 37°C in the dark. The cells were incubated with DAPI (1:10,000; cat. no. 4083; Cell Signaling Technology, Inc.) for 5 min at room temperature. Images of PTEN expression were acquired

(339) Plexina2 and CRMP2 Signaling Complex Is Activated by Nogo-A-Liganded Ngr1 to Restrict Corticospinal Axon Sprouting after TraumaThe Journal of neuroscience : the official journal of the Society for NeuroscienceApril 24, 2019Sekine Y, Algarate PT, Cafferty WBJ, Strittmatter SM.#R415, Life Technologies). Cell density was visualized using nuclear marker DAPI (0.1 ␮g/ml; catalog #4083, Cell Signaling Technology). Images were taken on a 10⫻ objective in an automated high-throughput imager

(340) Programmed death ligand-1, tumor infiltrating lymphocytes and HLA expression in Chinese extrahepatic cholangiocarcinoma patients: Possible immunotherapy implicationsBioScience TrendsMarch 14, 2019Fei Yu, Lei Gong, Zheng Mo, Wenran Wang, Meilong Wu, Jianghui Yang, Qiongqiong Zhang, Li Li, Jingjing Yao, Jiahong Dongantimouse-TRITC (ab6786, Abcam, Cambridge, UK). Nuclei were stained with 4,6-diamidino-2-phenylindole (#4083, Cell Signaling Technology, Danvers, MA, USA). Fluorescent images were analyzed with a fluorescence microscope

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DAPI

DAPI from Cell Signaling Technology

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DAPI from Cell Signaling Technology

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