DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(321) Intra-articularly injected mesenchymal stem cells promote cartilage regeneration, but do not permanently engraft in distant organsScientific reportsJuly 12, 2019Satué M, Schüler C, Ginner N, Erben RG.antibodies for 1 h at room temperature, air-dried, and mounted with Fluoromount (21644.01, Serva). DAPI (4083 S, Cell Signaling) was used to visualize nuclei. All immunofluorescence analyses were performed in a

(322) miR‑494‑3p regulates lipopolysaccharide‑induced inflammatory responses in RAW264.7 cells by targeting PTENMolecular medicine reportsMay 1, 2019Zhang S, He K, Zhou W, Cao J, Jin Z.Milbrae, CA, USA) for 1 h at 37°C in the dark. The cells were incubated with DAPI (1:10,000; cat. no. 4083; Cell Signaling Technology, Inc.) for 5 min at room temperature. Images of PTEN expression were acquired

(323) Plexina2 and CRMP2 Signaling Complex Is Activated by Nogo-A-Liganded Ngr1 to Restrict Corticospinal Axon Sprouting after TraumaThe Journal of neuroscience : the official journal of the Society for NeuroscienceApril 24, 2019Sekine Y, Algarate PT, Cafferty WBJ, Strittmatter SM.#R415, Life Technologies). Cell density was visualized using nuclear marker DAPI (0.1 ␮g/ml; catalog #4083, Cell Signaling Technology). Images were taken on a 10⫻ objective in an automated high-throughput imager

(324) Programmed death ligand-1, tumor infiltrating lymphocytes and HLA expression in Chinese extrahepatic cholangiocarcinoma patients: Possible immunotherapy implicationsBioScience TrendsMarch 14, 2019Fei Yu, Lei Gong, Zheng Mo, Wenran Wang, Meilong Wu, Jianghui Yang, Qiongqiong Zhang, Li Li, Jingjing Yao, Jiahong Dongantimouse-TRITC (ab6786, Abcam, Cambridge, UK). Nuclei were stained with 4,6-diamidino-2-phenylindole (#4083, Cell Signaling Technology, Danvers, MA, USA). Fluorescent images were analyzed with a fluorescence microscope

(325) MET mutation causes muscular dysplasia and arthrogryposisEMBO molecular medicineMarch 1, 2019Zhou H, Lian C, Wang T, Yang X, Xu C, Su D, Zheng S, Huang X, Liao Z, Zhou T, et al.Catalog No. #4413S). Nucleus was stained by DAPI in a final concentration of 0.1 μg/ml (CST, Catalog No. #4083). TUNEL assay was performed according to the manufacturer's instructions (MBL, Catalog No. 8445). Quantification

(326) 17β-Estradiol Regulates Glucose Metabolism and Insulin Secretion in Rat Islet β Cells Through GPER and Akt/mTOR/GLUT2 PathwayFrontiers in endocrinologyJanuary 1, 2019Bian C, Bai B, Gao Q, Li S, Zhao Y.Cell Signaling Technology, USA) at room temperature for 1 h. Sections were washed again and then DAPI stained (#4083, Cell Signaling Technology, USA) at room temperature for 10 min. Images of the sections were taken under

(327) Tumor-suppressive function of SIRT4 in neuroblastoma through mitochondrial damageCancer Management and ResearchDecember 7, 2018Yumei Wang, Yinmou Guo, Jianzhi Gao, Xiangdong Yuanwas added for 1 hour at room temperature. Cells were rinsed with PBST three times. Nuclear dye DAPI (4083; Cell Signaling Technology, Cambridge, London, UK) was added and cells were incubated in the dark for

(328) Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2Scientific ReportsDecember 1, 2018Shuqing Liu, Hong Jia, Shaohua Hou, Ting Xin, Xiaoyu Guo, Gaimei Zhang, Xintao Gao, Ming Li, Wuyang Zhu, Hongfei ZhuAfter incubation, the cells were washed once and resuspended in 50 μl of wash buffer containing DAPI (#4083; Cell Signaling) at 0.1 μg/ml. Fluorescent images were obtained (50,000 events per condition) using

(329) A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene TissuesG3 (Bethesda, Md.)November 6, 2018Huynh N, Zeng J, Liu W, King-Jones K.dilution buffer for 1 hr at room temperature, washed in 1x PBS3T and 1:50,000 DAPI (Cell Signaling 4083) for three times. Samples were mounted in Vectashield mounting medium (VECTH1000). Pictures were then

(330) High-Definition Analysis of Host Protein Stability during Human Cytomegalovirus Infection Reveals Antiviral Factors and Viral Evasion MechanismsCell host & microbeSeptember 12, 2018Nightingale K, Lin KM, Ravenhill BJ, Davies C, Nobre L, Fielding CA, Ruckova E, Fletcher-Etherington A, Soday L, Nichols H, et al.DMEM,Gibco,Cat#88364 Dialysed FBS,Gibco,Cat#24600044 Proline,Sigma-Aldrich,Cat#P5607 DAPI,Cell signaling,Cat#4083S ,, Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays ,, BCA Protein Assay Kit

(331) Brainstem Nuclei Associated with Mediating Apnea-Induced Respiratory Motor PlasticityScientific reportsAugust 23, 2018Lui S, Torontali Z, Tadjalli A, Peever J.RRID: AB_2313593). They were then counterstained with DAPI (1:1000 dilution, Cell Signalling, Cat# 4083 S, lot# 0007). Sections with viral vector were incubated in primary rabbit anti-mCherry (1:1000, Novus Biologicals

(332) The AMPK/p27^Kip1 Axis Regulates Autophagy/Apoptosis Decisions in Aged Skeletal Muscle Stem CellsStem cell reportsAugust 14, 2018White JP, Billin AN, Campbell ME, Russell AJ, Huffman KM, Kraus WE.A-21121) was used for detection. The cells were counterstained with DAPI (Cell Signaling, cat. no. 4083) and Phalloidin (Cell Signaling Technology, cat. no. 8953) to visualize nuclei and actin, respectively.

(333) Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammationEMBO molecular medicineAugust 1, 2018Zhao M, Xiong X, Ren K, Xu B, Cheng M, Sahu C, Wu K, Nie Y, Huang Z, Blumberg RS, et al.

(334) Stearic Acid Induces CD11c Expression in Proinflammatory Macrophages via Epidermal Fatty Acid Binding ProteinJournal of immunology (Baltimore, Md. : 1950)May 15, 2018Zeng J, Zhang Y, Hao J, Sun Y, Liu S, Bernlohr DA, Sauter ER, Cleary MP, Suttles J, Li B.anti-mouse CD11c (clone: N418, Biolegend cat#:117310, 1:200 dilution) and DAPI (Cell signaling cat# 4083S, 10mg/mL in H2O, 1:2000 dilution) were applied to the sections for 1h, followed by wash and sealing

(335) Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial TranslocationCell host & microbeApril 11, 2018Drolia R, Tenguria S, Durkes AC, Turner JR, Bhunia AK.BioChem Corp,Cat # LS002007 RNAlater,Thermo Fisher Scientific,Cat # AM7021 DAPI,Cell Signaling,Cat # 4083 FITC-labeled 4 kDa dextran,Sigma-Aldrich,Cat # 46944 PDTC,Sigma-Aldrich,Cat # P8765 ML-9,Sigma-Aldrich,Cat

(336) The nociceptin receptor inhibits axonal regeneration and recovery from spinal cord injuryScience signalingApril 3, 2018Sekine Y, Siegel CS, Sekine-Konno T, Cafferty WBJ, Strittmatter SM.Life Technologies). Cell density was visualized using the nuclear marker 4′,6-diamidino-2-phenylindole (0.1 μg/ml, #4083; Cell Signaling Technology). Images were taken on a 10× objective in an automated high-throughput imager

(337) Embryonic lethality in mice lacking Trim59 due to impaired gastrulation developmentCell death & diseaseFebruary 21, 2018Su X, Wu C, Ye X, Zeng M, Zhang Z, Che Y, Zhang Y, Liu L, Lin Y, Yang R.(ab175212, Abcam), anti-β-actin (SC-81178, Santa), Phalloidin-iFluor 488 (ab176753, Abcam), DAPI (#4083, Cell Signaling Technology), Alexa Fluor 488 Conjugate (#4416, #4408, Cell Signaling Technology), and

(338) bFGF plays a neuroprotective role by suppressing excessive autophagy and apoptosis after transient global cerebral ischemia in ratsCell death & diseaseFebruary 7, 2018Sun D, Wang W, Wang X, Wang Y, Xu X, Ping F, Du Y, Jiang W, Cui D.incubated with secondary antibodies for 2 h at room temperature. the nuclei were stained with DAPI (#4083, Cell Signaling Technology) for 3 min at temperature, and the slides were stored at 4 °C away from light. The

(339) BMSCs ameliorate septic coagulopathy by suppressing inflammation in cecal ligation and puncture-induced sepsisJournal of cell scienceJanuary 30, 2018Xu S, Zhou Z, Li H, Liu Z, Pan X, Wang F, Huang Y, Li X, Xiao Y, Pan J, et al.(1:200; Abcam, Cat# ab6717) and nuclei were stained with DAPI (1:200; Cell Signaling Technology, Cat# 4083) for 5 min. For cell immunofluorescence staining, macrophages were fixed with 4% paraformaldehyde at

(340) The Expression of Transcription Factors Mecp2 and CREB Is Modulated in Inflammatory Pelvic PainFrontiers in systems neuroscienceJanuary 1, 2018Xie AX, Pan XQ, Meacham RB, Malykhina AP.dark and then stained with DRAQ5 (Cell Signaling, #4084), 1:1,000 dilution or DAPI (Cell Signaling #4083), 1 μg/ml in PBS for 5 min at R/T after washed with PBS (Table 1). Sections were mounted in Prolong

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DAPI

DAPI from Cell Signaling Technology

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