DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(261) Ferroptosis in oligodendrocyte progenitor cells mediates white matter injury after hemorrhagic strokeCell death & diseaseMarch 23, 2022Shen D, Wu W, Liu J, Lan T, Xiao Z, Gai K, Hu L, Luo Z, Wei C, Wang X, et al.secondary antibodies for 1 h. Nuclei were stained with 1 μg/ml 4’,6-diamidino-2-phenylindole (DAPI, 4083 S, CST) for 10 min. Images were acquired by fluorescence microscope (Nikon ECLIPSE Ci) or confocal microscope

(262) Somatostatin-evoked Aβ catabolism in the brain: Mechanistic involvement of α-endosulfine-K(ATP) channel pathwayMolecular psychiatryMarch 1, 2022Watamura N, Kakiya N, Nilsson P, Tsubuki S, Kamano N, Takahashi M, Hashimoto S, Sasaguri H, Saito T, Saido TC.tyramide-enhanced FITC or rhodamine for 10 min. Finally, sections were treated with DAPI (Cell Signaling Technology 4083 S) diluted in TNB buffer before mounting with PermaFluor (Thermo Fisher Scientific TA-030-FM). Sections

(263) Diverse Macrophages Constituted the Glioma Microenvironment and Influenced by PTEN StatusFrontiers in ImmunologyFebruary 21, 2022Fengqi Zhou et al.for 1 h at room temperature, counterstained with 4,6-dianmino-2-phenylindole (DAPI) (Cell Signaling, 4083, 1:5,000), and sealed with mounting medium (Sigma-Aldrich, F4680). Statistical Analysis The differences

(264) DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3JCI insightFebruary 8, 2022Borza CM, Bolas G, Bock F, Zhang X, Akabogu FC, Zhang MZ, de Caestecker M, Yang M, Yang H, Lee E, et al.antibody (1:1000, Abcam, ab225225) for 12 hours at 4°C, then incubated with DAPI (1:200, Cell Signaling, 4083) in PBS for 10 minutes at room temperature. Slides were mounted with Prolong Gold Antifade reagent (Invitrogen,

(265) Exogenous melatonin prevents type 1 diabetes mellitus-induced bone loss, probably by inhibiting senescenceOsteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USAFebruary 1, 2022Gong Z, Da W, Tian Y, Zhao R, Qiu S, Wu Q, Wen K, Shen L, Zhou R, Tao L, et al.at room temperature for 1 h. After washing with cold PBS 3 times, the cells were stained with DAPI (4083, 0.1 μg/ml, CST, America) for 5 min. After washing with cold PBS 3 times, images were captured with

(266) Microengineered Multi-Organoid System from hiPSCs to Recapitulate Human Liver-Islet Axis in Normal and Type 2 DiabetesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)February 1, 2022Tao T, Deng P, Wang Y, Zhang X, Guo Y, Chen W, Qin J.secondary antibodies (1:500, CST) at room temperature for 1 h. Nuclei were stained with DAPI (1:4000, CST 4083). Real‐Time Quantitative PCR To quantify the gene expression levels in liver and islet organoids,

(267) Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosisNatureFebruary 1, 2022Deng W, Bai Y, Deng F, Pan Y, Mei S, Zheng Z, Min R, Wu Z, Li W, Miao R, et al.ImmunoResearch). Nuclei were counterstained with DAPI (4,6-diamidino-2-phenylindole) (Cell Signaling Technology, #4083). Slides were mounted using Aqua-Poly/Mount (Dako). Images were captured using a laser scanning confocal

(268) The Effects of Mechanical Loading Variations on the Hypertrophic, Anti-Apoptotic, and Anti-Inflammatory Responses of Differentiated Cardiomyocyte-like H9C2 CellsCellsJanuary 29, 2022Zevolis E, Philippou A, Moustogiannis A, Chatzigeorgiou A, Koutsilieris M.conjugated with CF 488A (1:2000, Biotium, Fremont CA, USA), and after staining also with DAPI (1 µg/mL; 4083; Cell Signaling), they were viewed under a microscope (Olympus BX40; Olympus Corporation, Tokyo, Japan).

(269) Mechanical force promotes dimethylarginine dimethylaminohydrolase 1-mediated hydrolysis of the metabolite asymmetric dimethylarginine to enhance bone formationNature communicationsJanuary 10, 2022Xie Z, Hou L, Shen S, Wu Y, Wang J, Jie Z, Zhao X, Li X, Zhang X, Chen J, et al.(1:1000; Molecular Probes, A21206) were used as secondary antibodies. DAPI (Cell Signaling Technology, #4083) and DyLight™ 594 Phalloidin (Cell Signaling Technology, #12877) were used for counterstaining. All

(270) PDIA2 Bridges Endoplasmic Reticulum Stress and Metabolic Reprogramming During Malignant Transformation of Chronic ColitisFrontiers in oncologyJanuary 1, 2022Tao J, Yin L, Wu A, Zhang J, Zhang J, Shi H, Liu S, Niu L, Xu L, Feng Y, et al.anti-rabbit IgG from Vector Laboratories (Burlingame, CA, USA), DAPI from Cell Signaling Technology (CST) (4083S, USA), and Annexin-FITC/PI from Shanghai Yisheng Biotechnology Co., Ltd (Shanghai, China). D (+)-glucose

(271) Primary Tumor Suppression and Systemic Immune Activation of Macrophages through the Sting Pathway in Metastatic Skin TumorYonsei medical journalJanuary 1, 2022Synn CB, Kim DK, Kim JH, Byeon Y, Kim YS, Yun MR, Lee JM, Lee W, Lee EJ, Lee S, et al.(PerkinElmer) according to the manufacturer’s protocol. The cell nuclei were stained with DAPI (cat no. #4083). The pIRF3 (cat no. #3033) and NF-κB p65 (cat no. #8242) antibodies were purchased from Cell Signaling Technology

(272) Three-dimensional analyses of vascular network morphology in a murine lymph node by X-ray phase-contrast tomography with a 2D Talbot arrayFrontiers in immunologyJanuary 1, 2022Schwarzenberg FL, Schütz P, Hammel JU, Riedel M, Bartl J, Bordbari S, Frank SC, Walkenfort B, Busse M, Herzen J, et al.10 min in 0.1% PBT and counterstained with DAPI as a nuclear counterstain (Cell Signaling, Cat.Nr.: 4083S, Cambridge, UK) 1:5000 in 0.1% PBT for 30 min. In slides where unconjugated antibodies were used, secondary

(273) miR-154-5p Affects the TGFβ1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1Oxidative medicine and cellular longevityJanuary 1, 2022Bian C, Luan Z, Zhang H, Zhang R, Gao J, Wang Y, Li J, Ren H.antibody,"Mouse antibody isotype control, #5415, for IP, Cell Signaling Technology" DAPI,"For nucleus, #4083, for IF, Cell Signaling Technology" Anti-rabbit IgG,"Anti-rabbit, #3678, for WB, Cell Signaling Technology" Anti-mouse

(274) Spontaneous formation of neutrophil extracellular traps is associated with autophagyScientific reportsDecember 14, 2021Guo Y, Gao F, Wang X, Pan Z, Wang Q, Xu S, Pan S, Li L, Zhao D, Qian J.min and exposed to ProLong Gold Antifade Reagent (Cell Signaling Technology, Beverly, USA, catalog # 4083S and catalog # 9071S, respectively). Cells were visualized using a confocal TCS SP8 microscope (Leica Microsystems

(275) Bioengineered miR-124-3p prodrug selectively alters the proteome of human carcinoma cells to control multiple cellular components and lung metastasis in vivoActa pharmaceutica Sinica. BDecember 1, 2021Deng L, Petrek H, Tu MJ, Batra N, Yu AX, Yu AM.anti-rabbit Alexa Fluor® 488-conjugated secondary antibody and then 4ʹ,6-diamidino-2-phenylindole (DAPI; #4083, Cell signaling Technology) for staining nuclei or Alexa Fluor® 594 Phalloidin (#12877, Cell signaling Technology

(276) Haem relieves hyperoxia-mediated inhibition of HMEC-1 cell proliferation, migration and angiogenesis by inhibiting BACH1 expressionBMC OphthalmologyDecember 1, 2021Lan Jian, Yang Mei, Chen Xing, Yuan Rongdito the instructions. Finally, the cells were washed with PBS for 5 min, stained with DAPI (1:1000, 4083, CST, USA) for 5 min, washed with PBS and sealed with an anti-fluorescence quenching solution [18].

(277) Meiotic Cas9 expression mediates gene conversion in the male and female mouse germlinePLoS biologyDecember 1, 2021Weitzel AJ, Grunwald HA, Weber C, Levina R, Gantz VM, Hedrick SM, Bier E, Cooper KL.anti-rabbit, 1:250 each] in block with 300 nM DAPI [Cell Signaling Technology (MA, USA), catalog # 4083S] and incubated at 4°C for 18 hours. We then washed 3× for 30′ in PBST and mounted under coverslips in

(278) p53 Promotes Differentiation of Cardiomyocytes from hiPSC through Wnt Signaling-Mediated Mesendodermal DifferentiationInternational journal of stem cellsNovember 30, 2021Liu Y, Zhang P, Huang W, Liu F, Long D, Peng W, Dang X, Zeng X, Zhou R.anti-mouse IgG (A-11005, Invitrogen, USA). The slides were then counterstained using 0.5 μg/ml of DAPI (#4083, Cell Signaling Technology, USA) for 15 min at room temperature. After rinsing with PBS, the chambers

(279) hPMSCs-Derived Exosomal miRNA-21 Protects Against Aging-Related Oxidative Damage of CD4+ T Cells by Targeting the PTEN/PI3K-Nrf2 AxisFrontiers in ImmunologyNovember 23, 2021Yanlian Xiong et al.dysfunction, thereby clinically alleviating immunosenescence. Materials And Methods Reagents DAPI(#4083) and antibodies against Histon H3 (#4499), Nrf2 (#12721), PI3K (#4249), Akt (#9272), β-actin (#4970),

(280) Schisandrin A protects against isoproterenol‑induced chronic heart failure via miR‑155Molecular Medicine ReportsNovember 22, 2021Lijing Gao, Ting Li, Shufen Li, Zhuohui Song, Yongli Chang, Li Yuanadded and incubated at 37°C for 1 h. The subsequent steps were performed in the dark. DAPI (cat. no. 4083; Cell Signaling Technology, Inc.) was used to incubate at room temperature for 5 min for nuclear counterstaining.

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DAPI

DAPI from Cell Signaling Technology

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