DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(181) Mitophagy Regulates the Circadian Rhythms by Degrading NR1D1 in Simulated Microgravity and Isolation EnvironmentsInternational Journal of Molecular SciencesApril 29, 2024Sihai Zhou, Xiaopeng Li, Fengji Liang, Guohua Ji, Ke Lv, Yanhong Yuan, Yujie Zhao, Na Yan, Chuanjie Zhang, Shiou Cai, et al.another three washes with PBS, the cells were incubated with DAPI (1:4000; cell signaling Technology; 4083, Danvers, MA, USA) in PBS for 5 min, followed by a final wash with PBS. Cell images were acquired using

(182) Cultivation, cryopreservation, and transcriptomic studies of host-adapted Cryptosporidium parvum and Cryptosporidium hominis using enteroidsiScienceApril 19, 2024Miner Deng, Tianyi Hou, Jie Zhang, Xinjie Mao, Fuxian Yang, Yanting Wei, Yongping Tang, Wanting Zeng, Wanyi Huang, Na Li, et al.CS10,STEMCELL,Cat#07930 BSA,Biofroxx,Cat#4240GR100 4% paraformaldehyde,Leagene,Cat#DF0135 Triton X-100,Sigma,Cat#9002-93-1 DAPI,CST,Cat# 4083S Advanced DMEM/F-12,Gibco,Cat#12634-010 DMEM,Gibco,Cat#c11995500bt DMEM/F-12,Gibco,Cat#11320033 ,, Critical

(183) Identification of Novel FZD4 Mutations in Familial Exudative Vitreoretinopathy and Investigating the Pathogenic Mechanisms of FZD4 MutationsInvestigative Opthalmology & Visual ScienceApril 1, 2024Erkuan Dai, Min Liu, Shujin Li, Xiang Zhang, Shiyuan Wang, Rulian Zhao, Yunqi He, Li Peng, Liting Lv, Haodong Xiao, et al.washed with PBS three times and then incubated with fluorescently labeled secondary antibodies and DAPI (4083; Cell Signaling Technology, Danvers, MA, USA) for one hour at room temperature. Fluorescence was observed

(184) Amyloid β oligomer induces cerebral vasculopathy via pericyte-mediated endothelial dysfunctionAlzheimer's research & therapyMarch 12, 2024Chen S, Guo D, Zhu Y, Xiao S, Xie J, Zhang Z, Hu Y, Huang J, Ma X, Ning Z, et al.Appropriate secondary antibodies (1:1000, Jackson, USA), 4,6-diamidino-2-phenylindole (DAPI, 1:1000, CST, 4083S, USA) and Lectin with DyLight™ 649 Lycopersicon Esculentum (Tomato) (Vector, DL-1178–1) were used for

(185) Investigating the Impact of Dimer Interface Mutations on Norrin's Secretion and Norrin/β-Catenin Pathway ActivationInvestigative ophthalmology & visual scienceMarch 5, 2024Liu M, Dai E, Yang M, Li S, Fan L, Liu Y, Xiao H, Zhao P, Yang Z.coverslips were washed with PBS 3 times and incubated with fluorescent secondary antibodies and DAPI (4083; CST) for 1 hour at room temperature. Fluorescence was visualized by LSM900 confocal microscope (Zeiss)

(186) Hapln1 promotes dedifferentiation and proliferation of iPSC-derived cardiomyocytes by promoting versican-based GDF11 trappingJournal of pharmaceutical analysisMarch 1, 2024Hao DJ, Qin Y, Zhou SJ, Dong BH, Yang JS, Zou P, Wang LP, Zhao YT.antibodies. The cells were then counterstained with 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (4083; Cell Signaling Technology) for 15 min at room temperature. After being washed with phosphate-buffered

(187) A mRNA Vaccine for Crimean–Congo Hemorrhagic Fever Virus Expressing Non-Fusion GnGc Using NSm Linker Elicits Unexpected Immune Responses in MiceVirusesFebruary 28, 2024Tong Chen, Zhe Ding, Xuejie Li, Yingwen Li, Jiaming Lan, Gary WongChina), diluted 1:1000 with PBS, were incubated for 1 h in darkness. After three washes with PBS, DAPI (4083, Cell Signaling Technology), diluted 1:10,000 with PBS, was incubated for 8 min in darkness. After two

(188) Tumor-derived extracellular vesicle drug delivery system for chemo-photothermal-immune combination cancer treatmentiScienceFebruary 16, 2024Yanghui Bi, Jieya Chen, Qing Li, Yan Li, Ling Zhang, Liu Zhida, Fajia Yuan, Ruiping Zhangproteins" ,, Albumin-bound paclitaxel,Hunan Kelun Pharmaceutical,H20203443 DAPI,Cell Signaling Technology,Cat#4083S Dil,Solarbio,Cat#D8700-10mg Dir,Solarbio,Cat#IC6110 Amiloride HCl dihydrate,Solarbio,Cat#A0080 Chlorpromazine

(189) Disease-associated AIOLOS variants lead to immune deficiency/dysregulation by haploinsufficiency and redefine AIOLOS functional domainsJ Clin InvestFebruary 1, 2024Hye Sun Kuehn, Inga S. Sakovich, Julie E. Niemela, Agustin A. Gil Silva, Jennifer L. Stoddard, Ekaterina A. Polyakova, Ana Esteve Sole, Svetlana N. Aleshkevich, Tatjana A. Uglova, Mikhail V. Belevtsev, et al.times in between each step. Cells were stained with DAPI (1 μg/mL, Cell signaling technology, Cat. 4083S) for 5 minutes, washed, mounted on slides using VECTASHIELD Mounting Medium (Vector Laboratories), and

(190) Reduced miR‐513a‐5p expression in COPD may regulate airway mucous cell hyperplasia through TFR1‐dependent signalingThe Kaohsiung Journal of Medical SciencesFebruary 1, 2024Jia Zhou, Jun‐Yi Du, Rui Xu, Xiao‐Juan Wu, Guo‐Yue ZhangMA) at a 1:500 dilution. After washing with PBS, the cell nuclei were stained with 0.5 μg/mL DAPI (#4083, Cell Signaling, Danvers, MA). After washing three times, the slides were embedded in 50% glycerol.

(191) FAM222A, Part of the BET-Regulated Basal Endothelial Transcriptome, Is a Novel Determinant of Endothelial Biology and Angiogenesis—Brief ReportArteriosclerosis, Thrombosis, and Vascular BiologyJanuary 1, 2024Aspasia Tzani, Stefan Haemmig, Henry S. Cheng, Daniel Pérez-Cremades, Marina Augusto Augusto Heuschkel, Anurag Jamaiyar, Sasha A. Singh, Masanori Aikawa, Paul B. Yu, Tianxi Wang, et al.Thermo Fisher). Cell nuclei were stained with 4′,6-diamidino- 2-phenylindole (DAPI, 0.5 ug/ml, Cat#: 4083, Cell Signaling). Retinas were imaged using a Zeiss AxioObserver.Z1 microscope and AxioCam MRm camera.

(192) Distinguished biomimetic dECM system facilitates early detection of metastatic breast cancer cellsBioengineering & Translational MedicineJanuary 1, 2024Bowen Weng, Mei Li, Weilai Zhu, Jing Peng, Xufeng Mao, Yanan Zheng, Chi Zhang, Senhao Pan, Haijiao Mao, Jiyuan Zhaostaining, the samples were fixed with 4% formaldehyde for 15 min and rinsed with PBS. DAPI solution (#4083, Cell Signaling Technology) at the concentration of 1 μg/mL was added, and the samples were incubated

(193) Wilms' tumor 1 (WT1) antigen is overexpressed in Kaposi Sarcoma and is regulated by KSHV vFLIPPLoS pathogensJanuary 1, 2024Morales AE, Gumenick R, Genovese CM, Jang YY, Ouedraogo A, Ibáñez de Garayo M, Pannellini T, Patel S, Bott ME, Alvarez J, et al.apoptosis detection, cells were stained using Annexin V (Biolegend 640920) and DAPI (Cell Signaling Technology, 4083S). Statistics Statistical analyses were performed using GraphPad Prism 9.4.1 (GraphPad Software) and

(194) Identification of a novel aromatic-turmerone analog that activates chaperone-mediated autophagy through the persistent activation of p38Frontiers in cell and developmental biologyJanuary 1, 2024Motomura K, Ueda E, Boateng A, Sugiura M, Kadoyama K, Hitora-Imamura N, Kurauchi Y, Katsuki H, Seki T.from Biotex Laboratories (München, Germany). 4′, 6-Diamidino-2-phenylindole, dihydrochloride (DAPI, #4083) was purchased from Cell Signaling Technology (Danvers, MA, United States). 2.2 Synthesis of ar-turmerone

(195) Chemokine‑like receptor 1‑positive cells are present in the odontoblast layer in tooth tissue in rats and humansExperimental and Therapeutic MedicineDecember 27, 2023Daisuke Yokokawa, Naoki Umemura, Yuka Miyamoto, Nobuo Kondoh, Satoshi KawanoCell Signaling Technology, Inc.) were added, incubated in the dark for 1 h and counterstained with DAPI (cat. no. 4083, 1:2,000; Cell Signaling Technology, Inc.) for 5 min in the dark at room temperature. After dehydration

(196) AMPK activation induces immunogenic cell death in AMLBlood advancesDecember 26, 2023Mondesir J, Ghisi M, Poillet L, Bossong RA, Kepp O, Kroemer G, Sarry JE, Tamburini J, Lane AA.(ThermoFisher Scientific, no. BDB556463); 4′,6-diamidino-2-phenylindole (Cell Signaling Technology, no. 4083S); Zombie Green (BioLegend, no. 423111); Zombie Aqua (BioLegend, no. 423101); anti-CALR antibody, ER

(197) Differential LRRK2 Signalling and Gene Expression in WT-LRRK2 and G2019S-LRRK2 Mouse Microglia Treated with Zymosan and MLi2CellsDecember 26, 2023Iqra Nazish, Adamantios Mamais, Anna Mallach, Conceicao Bettencourt, Alice Kaganovich, Thomas Warner, John Hardy, Patrick A. Lewis, Jennifer Pocock, Mark R. Cookson, et al.Clara, USA,#GA524), and anti- 4′,6-diamidino-2-phenylindole (DAPI) (Cell Signaling, Danfoss, MA, USA, #4083). The primary microglial yield was approximately 5 × 105 cells/flask with minimal astrocyte contamination.

(198) Natural Fatty Acid Guards against Brain Endothelial Cell Death and Microvascular Pathology following Ischemic Insult in the Presence of Acute HyperglycemiaBiomedicinesDecember 18, 2023Zaib Ali Shaheryar, Mahtab Ahmad Khan, Huma Hameed, Muhammad Naveed Mushtaq, Sajjad Muhammad, Gamal A. Shazly, Ali Irfan, Yousef A. Bin JardanMA, USA; and Goat anti-rabbit (1:1000), Proteintech, respectively. To stain the nuclei, we used DAPI #4083; 1:500; Cell Signaling Technology (Cambridge, UK). For 4-HNE staining, we incubated brain slices with

(199) Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modelingStem Cell ReportsDecember 12, 2023Atefeh Namipashaki, Kealan Pugsley, Xiaodong Liu, Kirra Abrehart, Sue Mei Lim, Guizhi Sun, Marco J. Herold, Jose M. Polo, Mark A. Bellgrove, Ziarih Hawipenicillin-streptomycin (Gibco, cat. no. 15140122), 5 μL of DAPI (Cell Signaling Technology, cat. no. 4083S), and 2 μL of 0.5 M EDTA (Invitrogen, cat. no. 15575020) and passed through a Falcon 5 mL tube with

(200) Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosisActa Pharmaceutica Sinica BDecember 1, 2023Wenjing Yu, Yuehuai Hu, Zhiping Liu, Kaiteng Guo, Dinghu Ma, Mingxia Peng, Yuemei Wang, Jing Zhang, Xiaolei Zhang, Panxia Wang, et al.with fluorescent secondary antibodies (4412S, 8890S; CST) for 1 h at room temperature. Finally, DAPI (4083; CST) was used to label the nuclei. Fluorescence was detected below the FV3000 fluorescent microscope

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DAPI

DAPI from Cell Signaling Technology

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DAPI from Cell Signaling Technology

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