DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(241) The NALCN channel regulates metastasis and nonmalignant cell disseminationNature geneticsDecember 1, 2022Rahrmann EP, Shorthouse D, Jassim A, Hu LP, Ortiz M, Mahler-Araujo B, Vogel P, Paez-Ribes M, Fatemi A, Hannon GJ, et al.Thermo Fisher Scientific, 1:500). Sections were counterstained (4,6-diamidino-2-phenylindole (DAPI); catalog number 4083, Cell Signaling, 1:10,000) and images captured using a Zeiss ImagerM2 and Apotome microscope or Zeiss

(242) Albumin alleviated esketamine-induced neuronal apoptosis of rat retina through downregulation of Zn2+-dependent matrix metalloproteinase 9 during the early developmentBMC NeuroscienceNovember 16, 2022Kan Zhang, Ruijing Ma, Luping Feng, Peiwen Liu, Shuang Cai, Chaoyang Tong, Jijian ZhengAlexa Fluor 488 (1:500, Abcam ab150109). Sections were incubated with DAPI (1:1000, Cell Signaling 4083 S) for 15 min. Fluorescent images were visualized using a confocal microscope (Leica TCS SP8). Images

(243) FBXO42 facilitates Notch signaling activation and global chromatin relaxation by promoting K63-linked polyubiquitination of RBPJScience AdvancesSeptember 23, 2022Hua Jiang, Weixiang Bian, Yue Sui, Huanle Li, Han Zhao, Wenqi Wang, Xu LiRRID:AB_2630356) at room temperature for 1 hour, and subjected to 4′,6-diamidino-2-phenylindole (DAPI) staining (4083S, CST). Coverslips were mounted using FluorSave reagent (345789, Millipore). The cells were viewed using

(244) AKR1C1 Protects Corneal Epithelial Cells Against Oxidative Stress-Mediated Ferroptosis in Dry EyeInvestigative Opthalmology & Visual ScienceSeptember 6, 2022Xin Zuo, Hao Zeng, Bowen Wang, Xue Yang, Dalian He, Li Wang, Hong Ouyang, Jin Yuananti–rabbit IgG (#4412), Alexa Fluor 488-labeled donkey anti-mouse IgG (#4409) antibodies, and DAPI (#4083) were purchased from Cell Signaling Technology (Danvers, MA, USA). The following antibodies were purchased

(245) A positive mechanobiological feedback loop controls bistable switching of cardiac fibroblast phenotypeCell discoverySeptember 6, 2022Niu L, Cheng B, Huang G, Nan K, Han S, Ren H, Liu N, Li Y, Genin GM, Xu F.by Rhodamine phalloidin (1:500; Invitrogen, R415, USA). DAPI (0.5 μg/mL; Cell Signaling Technology, 4083, USA) was used to stain cell nuclei. Immunofluorescent staining for YAP/F-actin/DAPI was used to calculate

(246) The Biphasic Effect of Retinoic Acid Signaling Pathway on the Biased Differentiation of Atrial-like and Sinoatrial Node-like Cells from hiPSCInternational journal of stem cellsAugust 30, 2022Liu F, Long D, Huang W, Peng W, Lan H, Zhou Y, Dang X, Zhou R.Invitrogen, USA). The cells were washed 3 times with PBS and then counterstained using 0.5 μg/ml of DAPI (4083, Cell Signaling Technology, USA) for 15 min at RT. The chambers were mounted and visualized under fluorescence microscopy

(247) Quinolizidines as Novel SARS-CoV-2 Entry InhibitorsInternational Journal of Molecular SciencesAugust 25, 2022Li Huang, Lei Zhu, Hua Xie, Jeffery Shawn Goodwin, Tanu Rana, Lan Xie, Chin-Ho Chenwith Prolong® Gold Anti-Fade Reagent with DAPI (Cell Signaling Technology, Danvers, MA, USA, Cat. # 4083S). Confocal images were acquired using a Nikon TE2000-U laser-scanning confocal microscope (Nikon, Tokyo,

(248) Transplantation of insulin-producing cells derived from human mesenchymal stromal/stem cells into diabetic humanized miceStem cell research & therapyJuly 26, 2022Ghoneim MA, Gabr MM, Refaie AF, El-Halawani SM, Al-Issawi MM, Elbassiouny BL, Kader MAAE, Ismail AM, Zidan MF, Karras MS, et al.anti-rabbit IgG (H + L) (# 4413, Cell Signaling Technology). The nuclei were counterstained with DAPI (# 4083, Cell Signaling Technology). For intracellular staining for flow cytometry: Primary monoclonal antibodies

(249) Single-cell RNA sequencing of subcutaneous adipose tissues identifies therapeutic targets for cancer-associated lymphedemaCell discoveryJune 21, 2022Liu X, Yuan M, Xiang Q, Li Z, Xu F, Chen W, Chen J, Huang J, Yu N, Zhou Z, et al.Fluor 488 goat anti-rabbit (A-11008, Invitrogen) secondary antibody at room temperature for 1 h. DAPI (4083S, Cell Signaling Technology) was used for nuclear counterstaining. The cells were imaged using the Axio

(250) miR‐181b regulates vascular endothelial aging by modulating an MAP3K3 signaling pathwayThe FASEB JournalJune 1, 2022Haoyang Zhou, Dafeng Yang, Henry S. Cheng, Michael G. McCoy, Daniel Pérez‐Cremades, Stefan Haemmig, Danny Wong, Lei Chen, Mark W. Feinbergtemperature. Nuclei were stained for 5min at room temperature in PBS containing DAPI (Cell signaling, 4083, 0.5μg/ml). Coverslips were mounted with ProLong Gold antifade reagent (Invitrogen). Images were acquired

(251) A novel frameshift variant in the TSPAN12 gene causes autosomal dominant FEVRMolecular genetics & genomic medicineJune 1, 2022Peng L, Dai E, Xiao H, Zhao R, He Y, Li S, Yang M, Yang Z, Zhao P.Plus 488 secondary antibody (1:300 dilution; CAT# A32723; Invitrogen) and DAPI (1:500 dilution; CAT# 4083; Cell Signaling Technology). Images were captured on an LSM 800 confocal scanning microscope (Zeiss,). 3

(252) miR-181b regulates vascular endothelial aging by modulating a MAP3K3 signaling pathwayFASEB JJune 1, 2022Haoyang Zhou, Dafeng Yang, Henry S. Cheng, Michael G McCoy, Daniel Pérez-Cremades, Stefan Haemmig, Danny Wong, Lei Chen, Mark W. Feinbergtemperature. Nuclei were stained for 5min at room temperature in PBS containing DAPI (Cell signaling, 4083, 0.5μg/ml). Coverslips were mounted with ProLong Gold antifade reagent (Invitrogen). Images were acquired

(253) Ganoderma Lucidum Triterpenoids Improve Maternal Separation-Induced Anxiety- and Depression-like Behaviors in Mice by Mitigating Inflammation in the Periphery and BrainNutrientsMay 28, 2022Xue Mi, Gui-Rong Zeng, Jie-Qing Liu, Zhou-Song Luo, Ling Zhang, Xiao-Man Dai, Wen-Ting Fang, Jing Zhang, Xiao-Chun Chenat 4 °C overnight and at room temperature for 1 h. The brain sections were exposed to DAPI (1 μg/mL; 4083, CST, Danvers, MA, USA) for nuclear staining for 5 min, and then cover slips were mounted on slides

(254) Comparative Metabolomics Analysis Reveals Key Metabolic Mechanisms and Protein Biomarkers in Alzheimer’s DiseaseFrontiers in PharmacologyMay 25, 2022Zhao Dai et al.37°C for 2 h. The slices were stained with DAPI nuclear staining (Cell Signaling Technology, cat. #4083) for 1 min. Use the same antibody as for western blot. 2.5 Network Visualization and Statistical Analysis Statistical

(255) Mapping brain-wide excitatory projectome of primate prefrontal cortex at submicron resolution and comparison with diffusion tractographyeLifeMay 20, 2022Yan M, Yu W, Lv Q, Lv Q, Bo T, Chen X, Liu Y, Zhan Y, Yan S, Shen X, et al.Alexa Fluor-conjugated secondary antibodies at room temperature for 3 hr, and DAPI (Cell signaling Cat# 4083 s) for 30 min at room temperature. The following primary antibodies were used: CaMKIIa (1:200, Abcam,

(256) Brain Extract of Subacute Traumatic Brain Injury Promotes the Neuronal Differentiation of Human Neural Stem Cells via AutophagyJournal of Clinical MedicineMay 11, 2022Zhenghui He et al.MA, USA) were used as secondary antibodies. Nuclei were counterstained with DAPI stain (1:10,000; #4083, Cell Signaling Technology, Danvers, MA, USA). The slides were viewed using the Zeiss Axio observer

(257) Treatment of SARS-CoV-2-induced pneumonia with NAD(+) and NMN in two mouse modelsCell discoveryApril 29, 2022Jiang Y, Deng Y, Pang H, Ma T, Ye Q, Chen Q, Chen H, Hu Z, Qin CF, Xu Z.human IgG (1:2000; Invitrogen). Nuclei were counterstained with DAPI (Cell Signaling Technology, Cat# 4083s). Fluorescent images were captured using a confocal laser-scanning microscope (Carl Zeiss, LSM700 and

(258) Coupled small molecules target RNA interference and JAK/STAT signaling to reduce Zika virus infection in Aedes aegyptiPLOS PathogensApril 4, 2022Chasity E. Trammell, Gabriela Ramirez, Irma Sanchez-Vargas, Laura A. St Clair, Oshani C. Ratnayake, Shirley Luckhart, Rushika Perera, Alan G. Goodmanmembranes for 1 h at room temperature in the dark. Samples were stained with DAPI (1:100; Cell Signaling 4083), mounted onto coverslips using ProLong Diamond Antifade Mountant (Invitrogen P36961), and imaged using

(259) Effectiveness of NLRP3 Inhibitor as a Non-Hormonal Treatment for ovarian endometriosisReproductive biology and endocrinology : RB&EMarch 29, 2022Murakami M, Osuka S, Muraoka A, Hayashi S, Bayasula, Kasahara Y, Sonehara R, Hariyama Y, Shinjo K, Tanaka H, et al.for 1 h at room temperature. Nuclear staining was carried out using 4',6-diamidino-2-phenylindole (4083, 1:1000, Cell Signaling Technology). Visualization was performed using a confocal laser-scanning microscope

(260) The Effect of Ex Vivo Human Serum from Liver Disease Patients on Cellular Protein Synthesis and GrowthCellsMarch 24, 2022Allen SL, Seabright AP, Quinlan JI, Dhaliwal A, Williams FR, Fine NHF, Hodson DJ, Armstrong MJ, Elsharkaway AM, Greig CA, et al.A32731, Thermo Fisher Scientific) as previously described [18]. Nuclei were stained with DAPI (1:5000, 4083, Cell Signaling Technology (CST), Leiden, The Netherlands). Image acquisition was performed using a

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DAPI

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